In vivo measurement of brain metabolites using two-dimensional double-quantum MR spectroscopy--exploration of GABA levels in a ketogenic diet.

Abstract:

:A localized proton 2D double-quantum (DQ) spin-echo spectroscopy technique was implemented on 1.5 T clinical MRI scanners for the detection of gamma-aminobutyrate (GABA) in the brain. The 2D approach facilitates separation of peaks overlapping with GABA in 1D DQ-filtered (DQF) spectra. This technique was applied to four normal adult volunteers and four children with intractable epilepsy. The coefficient of variation of the level of GABA and overlapping macromolecules at F2 = 3.0 ppm and F1 = 4.8 ppm was 0.08 in normal subjects. Three patients received 2D MRS scans before and after initiation of the ketogenic diet (KD): one patient showed a trend of decreasing GABA throughout the study, and two patients showed low initial GABA levels that increased over time. In addition to major metabolites and GABA, low-level metabolites (valine, leucine, and glutathione) were also identified in the 2D spectra.

journal_name

Magn Reson Med

authors

Wang ZJ,Bergqvist C,Hunter JV,Jin D,Wang DJ,Wehrli S,Zimmerman RA

doi

10.1002/mrm.10429

subject

Has Abstract

pub_date

2003-04-01 00:00:00

pages

615-9

issue

4

eissn

0740-3194

issn

1522-2594

journal_volume

49

pub_type

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