Different sialyltransferase activities in human colorectal carcinoma cells from surgical specimens detected by specific glycoprotein and glycolipid acceptors.


:The amount and type of sialylation of tumor cell membranes depends on the activity of a number of different sialyltransferase enzymes. For the detection of specific activities in human colorectal carcinoma tissue several glycoprotein and glycolipid acceptors were used: desialylated fetuin, alpha 1-acid glycoprotein, beta 2-glycoprotein I, ovine submaxillaris mucin, and the gangliosides GM1, GM2, GM3 and GD1a. Because of their possible relevance for metastasis, precursors of Le(a) and Le(x) antigens, too, were employed, namely neoglycolipids produced by coupling LcOse4 or NeoLcOse4 oligosaccharides to L-alpha-phosphatidyl-ethanol-amine-dipalmitoyl. Our data indicate that human colorectal tumor tissue contains two highly active sialyltransferase enzymes, which are only weakly expressed in normal mucosa. These are a N-glycan-specific alpha 2,6-sialyltransferase, which was significantly increased in metastasizing tumors, and a Gal beta 1,3Gal-NAc-specific sialyltransferase, which was increased in tumors of early stages. A shift to enhanced alpha 2,6-sialylation of membrane glycoproteins during carcinogenesis was demonstrated by lectin ELISA analysis of magneto-bead separated tumor cells. Quantitative determination of specific sialyltransferase activities may be a sensitive tool for detection and monitoring of colon carcinoma.


Clin Exp Metastasis


Kemmner W,Krück D,Schlag P




Has Abstract


1994-05-01 00:00:00












  • RANK expression on breast cancer cells promotes skeletal metastasis.

    abstract::RANK ligand (RANKL), acting through its cognate receptor RANK, is a key factor for bone remodeling and metastasis by regulating the differentiation, survival and activation of osteoclasts. RANKL is also crucial for the development of mouse mammary glands during pregnancy and has been recently linked to the etiology of...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Blake ML,Tometsko M,Miller R,Jones JC,Dougall WC

    更新日期:2014-02-01 00:00:00

  • Investigation of the role of signal transduction in attachment of ocular melanoma cells to matrix proteins: inhibition of attachment by calmodulin antagonists including tamoxifen.

    abstract::We investigated the role of signal transduction systems in the attachment of human uveal melanoma cells to matrix proteins. Ocular melanoma cells established from primary tumours attached rapidly to all substrates examined. Preferred substrates of attachment were collagens type I, III and IV and fibronectin rather tha...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Mac Neil S,Wagner M,Rennie IG

    更新日期:1994-11-01 00:00:00

  • The lack of correlation between experimental metastatic potential and platelet aggregating activity of B16 melanoma clones viewed in relation to tumor cell heterogeneity.

    abstract::Two widely used B16 melanoma cell lines of low and high lung colonizing potential (B16-F1 and B16-F10) were compared in their ability to induce platelet aggregation. The results of these experiments showed a reproducible difference in platelet aggregating activity of these two cell lines which directly correlated with...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Kimura AK,Mehta P,Xiang JH,Lawson D,Dugger D,Kao KJ,Lee-Ambrose L

    更新日期:1987-04-01 00:00:00

  • Estradiol increases ER-negative breast cancer metastasis in an experimental model.

    abstract::Breast cancer (BC) is the most common cancer affecting women in the United States and metastatic breast cancer is the leading cause of death. The role estradiol plays in ER-positive BC is well-documented, but the way it contributes to ER-negative BC remains unclear. In the present study, we utilized an experimental mo...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Yang X,Belosay A,Du M,Fan TM,Turner RT,Iwaniec UT,Helferich WG

    更新日期:2013-08-01 00:00:00

  • Bladder metastasis from renal cell carcinoma: retrospective analysis of 65 reported cases.

    abstract::This study was carried out to clarify the presentation, treatment options, and prognosis of renal cell carcinoma (RCC) metastasis to the bladder in which we do not yet have a comprehensive understanding. A systematic Medline, Web of Science, Embase, Google, and Ichushi Web search was performed to identify articles des...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Matsumoto K,Hayakawa N,Nakamura S,Oya M

    更新日期:2015-02-01 00:00:00

  • Silencing of skeletal metastasis-associated genes impairs migration of breast cancer cells and reduces osteolytic bone lesions.

    abstract::Bone sialoprotein (BSP) and osteopontin (OPN) are important factors in the metastasis of breast cancer, which were examined as targets for antineoplastic therapy by siRNA. In addition, the effect of gene silencing on their transcription factor Runx2 and their interaction partners integrin β(3) and matrix metalloprotei...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Reufsteck C,Lifshitz-Shovali R,Zepp M,Bäuerle T,Kübler D,Golomb G,Berger MR

    更新日期:2012-06-01 00:00:00

  • Therapeutic efficacy of two different cytostatic-linked phosphonates in combination with razoxane in the transplantable osteosarcoma of the rat.

    abstract::Two new compounds, 4-[4-[bis-(2-chloroethyl)-amino-]phenyl]-1- hydroxybutane-1,1-bisphosphonic acid (BAD) and aminotris-(methylenephosphonato)-diamminoplatinum(II) (ADP) that both have cytostatic and osteotropic properties, have shown good therapeutic efficacy against an osteosarcoma which metastasizes and kills by lu...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Klenner T,Wingen F,Keppler B,Valenzuela-Paz P,Amelung F,Schmähl D

    更新日期:1990-07-01 00:00:00

  • Lymphatic metastasis in breast cancer: importance and new insights into cellular and molecular mechanisms.

    abstract::Lymph node metastasis is the main prognosis factor in a number of malignancies, including breast carcinomas. The means by which lymph node metastases arise is not fully understood, and many questions remain about their importance in the further spread of breast cancer. Nevertheless, a number of key cellular and molecu...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Eccles S,Paon L,Sleeman J

    更新日期:2007-01-01 00:00:00

  • Role of simvastatin in tumor lymphangiogenesis and lymph node metastasis.

    abstract::Lymphangiogenesis plays a crucial role in promoting cancer metastasis to sentinel lymph nodes (LNs) and beyond. Increasing data have shown that simvastatin, a cholesterol-lowering medication for the prevention of cardiovascular diseases, is involved in tumor growth and dissemination, and endothelial functions. This st...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Ji RC,Eshita Y,Kobayashi T,Hidano S,Kamiyama N,Onishi Y

    更新日期:2018-12-01 00:00:00

  • Expression of 72 kDa and 92 kDa type IV collagenases from human giant-cell tumor of bone.

    abstract::Basement membrane forms widespread barriers to tumor invasion. It has been shown that tumor-secreted, basement membrane-degrading enzymes, namely metalloproteinases (MMPs) play an important role in tumor invasion and metastasis. In this study, we determined the enzymatic activity, content, and mRNA of both the 72 kDa ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Rao VH,Bridge JA,Neff JR,Schaefer GB,Buehler BA,Vishwanatha JK,Pollock RE,Nicolson GL,Yamamoto M,Gokaslam ZL

    更新日期:1995-11-01 00:00:00

  • Potentiation of antitumor effect of NKT cell ligand, alpha-galactosylceramide by combination with IL-12 on lung metastasis of malignant melanoma cells.

    abstract::The combined therapeutic effect of natural killer T (NKT) cell ligand alpha-galactosylceramide (alpha-GalCer) and IL-12 against highly metastatic B16-BL6-HM melanoma cells was investigated. In comparison with a single administration of alpha-GalCer or IL-12, the combined treatment of tumor-bearing mice with alpha-GalC...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Nakui M,Ohta A,Sekimoto M,Sato M,Iwakabe K,Yahata T,Kitamura H,Koda T,Kawano T,Makuuchi H,Taniguchi M,Nishimura T

    更新日期:2000-01-01 00:00:00

  • Potentiation of cell invasion and matrix metalloproteinase production by alpha3beta1 integrin-mediated adhesion of gastric carcinoma cells to laminin-5.

    abstract::We previously reported that the adhesion of gastric carcinoma cells to the peritoneum mediated by the alpha3beta1 integrin-laminin interaction is a key step in the initial process of peritoneal metastatic dissemination. Carcinoma cells subsequently invade through the intercellular gaps of mesothelial linings. In this ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Saito Y,Sekine W,Sano R,Komatsu S,Mizuno H,Katabami K,Shimada K,Oku T,Tsuji T

    更新日期:2010-04-01 00:00:00

  • An improved method to study NK-independent mechanisms of MTLn3 breast cancer lung metastasis.

    abstract::To study the tumor cell autonomous processes of metastasis, an in vivo tumor metastasis model is required that excludes the involvement of the innate immune system. For this purpose we used the established syngeneic MTLn3 cell - Fischer 344 tumor model. MTLn3 cells are efficiently eradicated by NK cells in vivo. Using...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: van Nimwegen MJ,Verkoeijen S,Kuppen PJ,Velthuis JH,van de Water B

    更新日期:2007-01-01 00:00:00

  • Skeletal impact of 17β-estradiol in T cell-deficient mice: age-dependent bone effects and osteosarcoma formation.

    abstract::Estrogen (E2)-dependent ER+ breast cancer, the most common breast cancer subtype, is also the most likely to metastasize to bone and form osteolytic lesions. However, ER+ breast cancer bone metastasis human xenograft models in nude mice are rarely studied due to complexities associated with distinguishing possible tum...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Cheng JN,Frye JB,Whitman SA,Funk JL

    更新日期:2020-04-01 00:00:00

  • A chimeric antibody targeting CD147 inhibits hepatocellular carcinoma cell motility via FAK-PI3K-Akt-Girdin signaling pathway.

    abstract::CD147 is expressed at low levels in normal tissues but frequently highly expressed in a wide range of tumor types such as lung, breast, and liver and therefore it is a potentially unique therapeutic target for these diverse tumor types. We previously generated a murine antibody HAb18 which suppresses matrix met al.lop...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Wang Y,Yuan L,Yang XM,Wei D,Wang B,Sun XX,Feng F,Nan G,Wang Y,Chen ZN,Bian H

    更新日期:2015-01-01 00:00:00

  • Analysis of c-fos, c-jun, c-erbB1, c-erbB2 and c-myc in primary lung carcinomas and their lymph node metastases.

    abstract::The purpose of this study was to identify possible alterations in proto-oncogenes (c-fos, c-jun, c-erbB1, c-erbB2 and c-myc) at the protein level in primary lung carcinomas and simultaneous metastatic lymph nodes of 21 patients. The analysis showed that proteins of c-jun and c-myc were expressed in a significantly hig...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Volm M,van Kaick G,Mattern J

    更新日期:1994-07-01 00:00:00

  • miR-200c inhibits invasion and migration in human colon cancer cells SW480/620 by targeting ZEB1.

    abstract::MicroRNAs are a class of ≈22-nt noncoding single-strand RNAs regulating gene expression postscriptionally. Metastasis caused poor prognosis in colorectal cancer patients and half of the patients developed metastatic lesions when admission. Here we investigated the possible roles of microRNAs in regulating metastasis i...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Chen ML,Liang LS,Wang XK

    更新日期:2012-06-01 00:00:00

  • High-dose ionizing radiation-induced hematotoxicity and metastasis in mice model.

    abstract::Radiotherapy induces untargeted effects on normal tissues such as bone marrow. So alteration of microenvironment by ionizing irradiation is supposed to influence dynamic host-cancer ecosystem affecting cancer behavior including metastasis. Herein, the incidence of lung metastasis after high-dose irradiation has been i...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Shin JW,Son JY,Raghavendran HR,Chung WK,Kim HG,Park HJ,Jang SS,Son CG

    更新日期:2011-12-01 00:00:00

  • Effect of a perfluorochemical emulsion on the development of artificial lung metastases in mice.

    abstract::Intravenous infusions of perfluorochemical emulsions, combined with administration of inspired oxygen or carbogen have been found to improve tumor oxygenation and increase the response of solid tumors in animals to radiotherapy. Fluosol-DA 20 per cent, the only perfluorochemical emulsion currently approved for testing...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Rockwell S,Irvin CG,Nierenburg M

    更新日期:1986-01-01 00:00:00

  • The regulation of prostate cancer cell adhesion to human bone marrow endothelial cell monolayers by androgen dihydrotestosterone and cytokines.

    abstract::A previous study from our laboratory suggested that prostate cancer metastasis to bone may be mediated, in part, by preferential adhesion to human bone marrow endothelial (HBME) cells. Tumor cell adhesion to endothelial cells may be modulated by the effect of cytokines on cell adhesion molecules (CAMs). Tumor necrosis...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Cooper CR,Bhatia JK,Muenchen HJ,McLean L,Hayasaka S,Taylor J,Poncza PJ,Pienta KJ

    更新日期:2002-01-01 00:00:00

  • Adriamycin-induced inhibition of melanoma cell invasion is correlated with decreases in tumor cell motility and increases in focal contact formation.

    abstract::Tumor cell adhesion to the extracellular matrix (ECM) is closely linked with tumor cell invasion and metastasis. In this study, we demonstrate that low levels of adriamycin, a widely used anticancer drug, can inhibit the invasion of highly metastatic K1735-M2 mouse melanoma cells in vitro through a reconstituted basem...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Repesh LA,Drake SR,Warner MC,Downing SW,Jyring R,Seftor EA,Hendrix MJ,McCarthy JB

    更新日期:1993-01-01 00:00:00

  • Resistance of the embryonic notochord to invasion by malignant neurogenic tumor cells in vitro.

    abstract::Very few tissues in vivo are resistant to invasion by malignant cells. In this paper, confrontation cultures consisting of chick embryonic notochord fragments and cell aggregates from three malignant neurogenic cell lines showed a resistance of the embryonic organ to malignant invasion by tumor cells. The mechanism of...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Schroyens W,Camon J,De Ridder L

    更新日期:1991-07-01 00:00:00

  • The challenge of drug resistance in cancer treatment: a current overview.

    abstract::It is generally accepted that recent advances in anticancer agents have contributed significantly to the improvement of both the disease-free survival and quality of life in cancer patients. However, in many instances, a favorable initial response to treatment changes afterwards, thereby leading to cancer relapse and ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Nikolaou M,Pavlopoulou A,Georgakilas AG,Kyrodimos E

    更新日期:2018-04-01 00:00:00

  • Dendritic cells recruitment in melanoma metastasis treated by electrochemotherapy.

    abstract::Electrochemotherapy (ECT) is a novel treatment for recurrent or in-transit unresectable melanoma metastases based on the administration of anti-neoplastic drugs followed by cancer cell electroporation. Whether ECT can also induce anti-tumour immunity is unclear. We addressed this issue investigating the presence of de...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Gerlini G,Sestini S,Di Gennaro P,Urso C,Pimpinelli N,Borgognoni L

    更新日期:2013-01-01 00:00:00

  • The meaning of the c-kit proto-oncogene product in malignant transformation in human mammary epithelium.

    abstract::To evaluate the relationship between the c-kit proto-oncogene product and malignant transformation of human breast tissue, we examined the immunohistochemical expression of the c-kit proto-oncogene product in both malignant and non-malignant breast tissues. The immunohistochemical expression of the c-kit proto-oncogen...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Ko CD,Kim JS,Ko BG,Son BH,Kang HJ,Yoon HS,Cho EY,Gong G,Ahn SH

    更新日期:2003-01-01 00:00:00

  • Inhibition of liver tumor cell colonization in two animal tumor models by lectin blocking with D-galactose or arabinogalactan.

    abstract::Repeated administration of the hepatic lectin blocking agents D-galactose or arabinogalactan completely prevented the settling of metastatic cells of sarcoma L-1 tumor in the liver of Balb/c mice and greatly reduced the colonization process of highly metastatic ESb lymphoma cells of the liver of DBA/2 mice. Therefore,...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Beuth J,Ko HL,Schirrmacher V,Uhlenbruck G,Pulverer G

    更新日期:1988-03-01 00:00:00

  • Modulation of clonal progression in B16F1 melanoma cells.

    abstract::We have examined the effects of the microenvironment on the frequency and generation of metastatic variant cells in both parental B16F1 melanoma cells and nascent clones. The metastatic abilities of cultured B16F1 cells were tested after a period of growth in the presence or absence of a second cell population separat...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Harris JF,Chambers AF,Tam AS

    更新日期:1991-03-01 00:00:00

  • Salvage treatment for recurrences after first resection of colorectal liver metastases: the impact of histopathological growth patterns.

    abstract::The majority of patients recur after resection of colorectal liver metastases (CRLM). Patients with CRLM displaying a desmoplastic histopathological growth pattern (dHGP) have a better prognosis and lower probability of recurrence than patients with non-dHGP CRLM. The current study evaluates the impact of HGP type on ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Nierop PMH,Galjart B,Höppener DJ,van der Stok EP,Coebergh van den Braak RRJ,Vermeulen PB,Grünhagen DJ,Verhoef C

    更新日期:2019-04-01 00:00:00

  • The protein kinase C inhibitor, H7, inhibits tumor cell invasion and metastasis in mouse melanoma via suppression of ERK1/2.

    abstract::Protein kinase C (PKC) has been shown to be a signal transducer during tumorigenesis, tumor cell invasion, and metastasis. Recent studies have reported that the PKC inhibitor, 7-hydroxystaurosporine, inhibits tumor cell invasion. However, the molecular mechanisms of this inhibition of invasion and metastasis are not w...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Tsubaki M,Matsuoka H,Yamamoto C,Kato C,Ogaki M,Satou T,Itoh T,Kusunoki T,Tanimori Y,Nishida S

    更新日期:2007-01-01 00:00:00

  • Expression of activated gelatinase in human invasive breast carcinoma.

    abstract::The expression of both 92- and 72-kDa gelatinases has been studied in 20 samples of human breast carcinoma by the technique of gelatin zymography. This technique allowed the relative amount of each gelatinase to be determined in small samples of tissue (< 10 mg). More importantly, active and latent forms of the two ge...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Brown PD,Bloxidge RE,Anderson E,Howell A

    更新日期:1993-03-01 00:00:00