当前位置： SCI文献检索 > CLINICAL & EXPERIMENTAL METASTASIS期刊下所有文献 > A novel in vitro assay system for transendothelial tumor cell invasion: significance of E-selectin and alpha 3 integrin in the transendothelial invasion by HT1080 fibrosarcoma cells.

A novel in vitro assay system for transendothelial tumor cell invasion: significance of E-selectin and alpha 3 integrin in the transendothelial invasion by HT1080 fibrosarcoma cells.

Abstract：

:The interaction of tumor cells with endothelial cells is a key event in tumor metastasis. We established an in vitro invasion assay system, in which the invasion of tumor cells after interaction with endothelial cells can be examined. Two chamber culture wells separated by porous membrane were used. Human umbilical vein endothelial cells (HUVEC) were placed on porous membranes coated with matrix components. The invasion by HT1080 fibrosarcoma cells was determined in this system by counting the number of cells that moved through the membranes from upper to lower chambers. HUVEC cells did not migrate through the membranes as judged by the staining with UEA-I. Observation by scanning electron microscopy revealed that HT1080 cells bound to HUVEC surfaces and migrated underneath the HUVEC monolayer. Effects of antibodies specific for cell surface adhesion molecules on the migration of HT1080 cells were examined. Invasion of uncoated membranes and membranes coated with HUVEC cells was compared. Antibody against E-selectin significantly suppressed an increase of HT1080 cell invasion of HUVEC monolayers stimulated by IL-1 beta or TNF alpha. Antibody against integrin alpha 3 subunit remarkably inhibited the invasion of HUVEC cell-coated membranes, suggesting that integrins with the alpha 3 subunit may play an important role in the transendothelial invasion by HT1080 cells.

journal_name

Clin Exp Metastasis

journal_title

Clinical & experimental metastasis

authors

Okada T,Okuno H,Mitsui Y

doi

10.1007/BF01753837

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

305-14

issue

4

eissn

0262-0898

issn

1573-7276

journal_volume

12

pub_type

杂志文章

相关文献

CLINICAL & EXPERIMENTAL METASTASIS文献大全
  • Levels of expression of endothelial markers specific to tumour-associated endothelial cells and their correlation with prognosis in patients with breast cancer.

    abstract：:Tumour endothelial markers (TEMs) are a newly discovered family of endothelial markers associated with tumour specific angiogenesis. This study sought to examine the levels of expression for TEMs in human breast cancer. Breast cancer tissues (n = 120) together with normal background tissues (n = 33) were obtained afte...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/b:clin.0000017168.83616.d0

    authors： Davies G,Cunnick GH,Mansel RE,Mason MD,Jiang WG

    更新日期：2004-01-01 00:00:00

  • The expression of neurokinin-1 and preprotachykinin-1 in breast cancer cells depends on the relative degree of invasive and metastatic potential.

    abstract：:Breast cancer has a predilection for metastasis to the bone marrow. The preprotachykinin-I (PPT-I) gene has a central role in the early migration of breast cancer cells into the bone marrow, making this organ a latent repository of the cancer cells. This study investigated whether the invasive and metastatic potential...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-006-9001-6

    authors： Castro TA,Cohen MC,Rameshwar P

    更新日期：2005-01-01 00:00:00

  • Metastasis of mammary tumours in mice: relationship with morphology of primary tumour, and reproductive background of host.

    abstract：:The lungs of BR6 breeding female mice which had developed spontaneous mammary tumours, were examined histologically for the presence of metastases. Metastatic nodules, or tumour cell emboli within blood vessels, were found in the lungs of 3 out of 42 (7 per cent) mice with pregnancy-dependent tumours, and in 33 out of...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/BF00736406

    authors： Lee AE,Pang LS,Rogers LA,Jeffery RE

    更新日期：1983-07-01 00:00:00

  • Multiple effects of transfection with interleukin 2 and/or interferon gamma on the behavior of mouse T lymphoma cells.

    abstract：:We have previously reported that transfection of mouse interferon gamma (IFN-gamma) in H-2Kk-positive BW variants (BW-Sp3) abolishes tumorigenicity and reduces metastatic capacity. To further increase the immunogenicity of BW-Sp3 cells, the gene for human interleukin 2 (huIL-2) was transfected in these cancer cells. S...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1006585525399

    authors： Geldhof AB,Van Ginderachter J,Vandersteen P,Raes G,De Baetselier P

    更新日期：1998-07-01 00:00:00

  • Early incorporated endothelial cells as origin of metastatic tumor vasculogenesis.

    abstract：:Vascularization of solid tumors is thought to occur by sprouting or intussusceptive angiogenesis, co-option of existing vessels, and vasculogenic mimicry after the onset of tumor hypoxia, when the tumor radius exceeds the oxygen diffusion distance. In contrast, here we show that individual endothelial cells that are i...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-009-9257-8

    authors： Elzarrad K,Haroon A,Reed D,Al-Mehdi AB

    更新日期：2009-01-01 00:00:00

  • Evidence for the causal role of endogenous interferon-alpha/beta in the regulation of angiogenesis, tumorigenicity, and metastasis of cutaneous neoplasms.

    abstract：:Primary tumor growth and metastasis depend on angiogenesis, which is determined by the balance between proangiogenic and antiangiogenic molecules. Interferon (IFN)-alpha and -beta inhibit angiogenesis through downregulation of interleukin-8, matrix metalloproteinase-9, and basic fibroblast growth factor. To provide ev...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1020923326441

    authors： McCarty MF,Bielenberg D,Donawho C,Bucana CD,Fidler IJ

    更新日期：2002-01-01 00:00:00

  • Distinctive alterations of invasiveness, drug resistance and cell-cell organization in 3D-cultures of MCF-7, a human breast cancer cell line, and its multidrug resistant variant.

    abstract：:Growth of human tumor cells as three-dimensional (3D) multicellular spheroids modifies their invasive properties. Here we study the differences in the biological features of MCF-7, a human breast cancer cell line, and its multidrug resistant variant (MDR-MCF-7) cultured as spheroids or as monolayers. Three-dimensional...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1014594825502

    authors： dit Faute MA,Laurent L,Ploton D,Poupon MF,Jardillier JC,Bobichon H

    更新日期：2002-01-01 00:00:00

  • The meaning of the c-kit proto-oncogene product in malignant transformation in human mammary epithelium.

    abstract：:To evaluate the relationship between the c-kit proto-oncogene product and malignant transformation of human breast tissue, we examined the immunohistochemical expression of the c-kit proto-oncogene product in both malignant and non-malignant breast tissues. The immunohistochemical expression of the c-kit proto-oncogen...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1027323210736

    authors： Ko CD,Kim JS,Ko BG,Son BH,Kang HJ,Yoon HS,Cho EY,Gong G,Ahn SH

    更新日期：2003-01-01 00:00:00

  • Control of pulmonary metastases of rat mammary cancer by inhibition of uPA and COX-2, singly and in combination.

    abstract：:Tumor cell metastasis can be suppressed by the attenuation of proteolytic and angiogenic events that are mediated by tumor and endothelial cells. Combinations of specific inhibitors directed to separate stages of the metastatic cascade may improve the potential for adjuvant therapies. Amiloride is an effective plasmin...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/b:clin.0000046140.19131.19

    authors： Evans DM,Sloan Stakleff KD

    更新日期：2004-01-01 00:00:00

  • MiR-193a-3p and miR-193a-5p suppress the metastasis of human osteosarcoma cells by down-regulating Rab27B and SRR, respectively.

    abstract：:MicroRNAs have been identified as key players in the development and progression of osteosarcoma, which is the most common primary malignancy of bone. Sequencing-based miR-omic and quantitative real-time PCR analyses suggested that the expression of miR-193a-3p and miR-193a-5p was decreased by DNA methylation at their...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-016-9783-0

    authors： Pu Y,Zhao F,Cai W,Meng X,Li Y,Cai S

    更新日期：2016-04-01 00:00:00

  • Solitary bone metastasis in the tibia as a presenting sign of endometrial adenocarcinoma: a case report and the review of the literature.

    abstract：BACKGROUND:Metastasis to bone from endometrial adenocarcinoma is rare, when metastasises it usually locates in axial skeleton. Metastasis to extremities is extremely rare. Additionally the detection of the bone metastasis as a presenting feature is uncommon. In the present study we report the 10th cases of bone metasta...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-007-9061-2

    authors： Kaya A,Olmezoglu A,Eren CS,Bayol U,Altay T,Karapinar L,Ozturk H,Oztekin D,Guvenli Y,Karadogan I

    更新日期：2007-01-01 00:00:00

  • Automated CT-based analysis to detect changes in the prevalence of lytic bone metastases from breast cancer.

    abstract：:The spinal column is the most frequent site of bone metastasis in patients with breast cancer. It is important to understand how the pattern of vertebral lesions may be affected by the introduction of modern cancer therapies. The purpose of this study was to characterize changes in the radiological appearance of spina...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-008-9219-6

    authors： Skrinskas T,Clemons M,Freedman O,Weller I,Whyne CM

    更新日期：2009-01-01 00:00:00

  • Trks are novel oncogenes involved in the induction of neovascularization, tumor progression, and nodal metastasis in oral squamous cell carcinoma.

    abstract：:The function of tropomyosin receptor kinase (Trk) family including TrkA, TrkB, and TrkC in cancer remains unknown. The role of Trks in oral squamous cell carcinoma (OSCC) was examined. Knockdown of Trks provided inhibition of growth or invasion and decrease of apoptosis in OSCC cells, which expressed Trks at high leve...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-012-9525-x

    authors： Sasahira T,Ueda N,Yamamoto K,Bhawal UK,Kurihara M,Kirita T,Kuniyasu H

    更新日期：2013-02-01 00:00:00

  • Impact of decompressive laminectomy on the functional outcome of patients with metastatic spinal cord compression and neurological impairment.

    abstract：:Metastatic spinal cord compression (MSCC) is a frequent phenomenon in advanced tumor diseases with often severe neurological impairments. Affected patients are often treated by decompressive laminectomy. To assess the impact of this procedure on Karnofsky Performance Index (KPI) and Frankel Grade (FG) at discharge, a ...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-019-10016-z

    authors： Younsi A,Riemann L,Scherer M,Unterberg A,Zweckberger K

    更新日期：2020-04-01 00:00:00

  • An improved method to study NK-independent mechanisms of MTLn3 breast cancer lung metastasis.

    abstract：:To study the tumor cell autonomous processes of metastasis, an in vivo tumor metastasis model is required that excludes the involvement of the innate immune system. For this purpose we used the established syngeneic MTLn3 cell - Fischer 344 tumor model. MTLn3 cells are efficiently eradicated by NK cells in vivo. Using...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-007-9075-9

    authors： van Nimwegen MJ,Verkoeijen S,Kuppen PJ,Velthuis JH,van de Water B

    更新日期：2007-01-01 00:00:00

  • Augmented autocrine bone morphogenic protein (BMP) 7 signaling increases the metastatic potential of mouse breast cancer cells.

    abstract：:As malignant breast cancers progress, they acquire the ability to spread to other regions of the body, including bone and lung, but the molecular mechanism underlying the increase in metastatic potential is not fully understood. Here we studied murine 4T1E/M3 highly bone marrow metastatic breast cancer cells, which we...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-012-9453-9

    authors： Sakai H,Furihata M,Matsuda C,Takahashi M,Miyazaki H,Konakahara T,Imamura T,Okada T

    更新日期：2012-04-01 00:00:00

  • IL-4 and TNF-alpha induce changes in integrin expression and adhesive properties and decrease the lung-colonizing potential of HT-29 colon carcinoma cells.

    abstract：:The colon carcinoma cell line HT-29 was used to explore the potential of interleukin-4 (IL-4) and tumor necrosis factor alpha (TNF-alpha) to modify integrin expression and adhesive functions of tumor cells in vitro and to examine corresponding metastatic effects in vivo. Preincubation of HT-29 cells with 100 U/ml of I...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/BF00121213

    authors： Herzberg F,Schöning M,Schirner M,Topp M,Thiel E,Kreuser ED

    更新日期：1996-03-01 00:00:00

  • GPR56 plays varying roles in endogenous cancer progression.

    abstract：:GPR56, a non-classical adhesion receptor, was previously reported to suppress tumor growth and metastasis in xenograft models using human melanoma cell lines. To understand whether GPR56 plays similar roles in the development of endogenous tumors, we analyzed cancer progression in Gpr56 (-/-) mice using a variety of t...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-010-9322-3

    authors： Xu L,Begum S,Barry M,Crowley D,Yang L,Bronson RT,Hynes RO

    更新日期：2010-04-01 00:00:00

  • 4-[3,5-Bis(trimethylsilyl)benzamido] benzoic acid (TAC-101) inhibits the intrahepatic spread of hepatocellular carcinoma and prolongs the life-span of tumor-bearing animals.

    abstract：:We examined the in vivo anti-tumor activity of the benzoic acid derivative, TAC-101 (4-[3,5-bis(trimethylsilyl)benzamido] benzoic acid), for intrahepatic spread of JHH-7 human hepatocellular carcinoma (HCC) cells and its mechanism of action. Oral administration of TAC-101 markedly inhibited liver tumor of JHH-7 cells ...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1006567229929

    authors： Murakami K,Matsuura T,Sano M,Hashimoto A,Yonekura K,Sakukawa R,Yamada Y,Saiki I

    更新日期：1998-10-01 00:00:00

  • Ascites-induced shift along epithelial-mesenchymal spectrum in ovarian cancer cells: enhancement of their invasive behavior partly dependant on αv integrins.

    abstract：:At least one-third of patients with epithelial ovarian cancer (OC) present ascites at diagnosis and almost all have ascites at recurrence. The presence of ascites, which acts as a dynamic reservoir of active molecules and cellular components, correlates with the OC peritoneal metastasis and is associated with poor pro...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-014-9658-1

    authors： Carduner L,Leroy-Dudal J,Picot CR,Gallet O,Carreiras F,Kellouche S

    更新日期：2014-08-01 00:00:00

  • Nitric oxide induced by tumor cells activates tumor cell adhesion to endothelial cells and permeability of the endothelium in vitro.

    abstract：:Human fibrosarcoma HT1080 cell surface phenotype analysis revealed the expression of "cluster of differentiation 15" (CD15) antigen and to a lesser extent, of "very late antigen-4" (VLA-4). Expression of "endothelial-leukocyte adhesion molecule-1" (ELAM-1) was negligible on resting human umbilical vascular endothelial...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1018487213157

    authors： Yudoh K,Matsui H,Tsuji H

    更新日期：1997-11-01 00:00:00

  • Stimulation of tumor cell growth in vitro by a monoclonal antibody to a tumor specific protein (TSP-180) present on the cell surface of 3LL cells.

    abstract：:Proliferation capacity and MHC class I antigen expression of two Lewis lung carcinoma (3LL) metastatic variants (C87, BC215) grown under defined experimental conditions (serum-free defined medium or 10 per cent serum) have been studied following exposure to MoAb 135-13C which recognizes on these cells a tumor surface ...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/BF02057180

    authors： Sacchi A,Piaggio G,Rizzo MA,Falcioni R,Kennel SJ

    更新日期：1989-01-01 00:00:00

  • Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis.

    abstract：:Metastasis is key to cancer mortality. Understanding its biology is vital for developing strategies to prevent and treat metastasis. Phenotypic assays to either study metastasis or evaluate anti-metastatic drugs are widely used in preclinical research. This technical note discusses the adherence of reporting essential...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-018-9944-4

    authors： Boiy R,Steenbrugge J,Van Deun J,Hendrix A,Meyer E,De Wever O

    更新日期：2018-12-01 00:00:00

  • Treatment with human recombinant leukocyte interferons inhibits in vitro invasive ability of human lung carcinoma cells.

    abstract：:The effects of treatment of human lung carcinoma cell line A 549 with recombinant DNA-derived human leukocyte interferons A (rIFN-alpha A) or D (rIFN-alpha D), and human lymphoblastoid interferon (Wellferon) on in vitro cell invasion were investigated in a quantitative invasion assay using human amnion. The A 549 cell...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/BF00117932

    authors： Ravine TJ,Ledinko N

    更新日期：1986-07-01 00:00:00

  • Graded hypoxia modulates the invasive potential of HT1080 fibrosarcoma and MDA MB231 carcinoma cells.

    abstract：:Spatial and temporal oxygen heterogeneity exists in most solid tumour microenvironments due to an inadequate vascular network supplying a dense population of tumour cells. An imbalance between oxygen supply and demand leads to hypoxia within a significant proportion of a tumour, which has been correlated to the likeli...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-007-9139-x

    authors： Subarsky P,Hill RP

    更新日期：2008-01-01 00:00:00

  • KiSS1 suppresses metastasis in human ovarian cancer via inhibition of protein kinase C alpha.

    abstract：:Metastasis is a vital target for cancer treatment, since the majority of cancer patients die from metastatic, rather than the primary disease. KiSS1 has been identified as a metastasis suppressor gene in melanoma and breast carcinomas. We show here that KiSS1 is also a metastasis suppressor in human ovarian cancer. Ov...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-005-8186-4

    authors： Jiang Y,Berk M,Singh LS,Tan H,Yin L,Powell CT,Xu Y

    更新日期：2005-01-01 00:00:00

  • Anti-metastatic properties of RGD-peptidomimetic agents S137 and S247.

    abstract：:Integrins expressed on endothelial cells modulate cell migration and survival during angiogenesis. Integrins expressed on carcinoma cells potentiate metastasis by facilitating invasion and movement across blood vessels. We describe the activities of two synthetic low-molecular-weight peptidomimetics of the ligand amin...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/b:clin.0000024764.93092.5f

    authors： Shannon KE,Keene JL,Settle SL,Duffin TD,Nickols MA,Westlin M,Schroeter S,Ruminski PG,Griggs DW

    更新日期：2004-01-01 00:00:00

  • Inhibition of colony formation in agarose of metastatic human breast carcinoma and melanoma cells by synthetic glycoamine analogs.

    abstract：:We studied the influence of 10 synthetic glycoamine analogs on colony formation in 0.3 and 0.9% agarose by metastatic human breast carcinoma (MDA-MB-435) and melanoma (TXM-13) cells. Nine synthetic analogs significantly inhibited the colony formation in 0.9% agarose of MDA-MB-435 human breast carcinoma cells; five com...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/BF00053899

    authors： Glinsky GV,Mossine VV,Price JE,Bielenberg D,Glinsky VV,Ananthaswamy HN,Feather MS

    更新日期：1996-05-01 00:00:00

  • Mapping protein signal pathway interaction in sarcoma bone metastasis: linkage between rank, metalloproteinases turnover and growth factor signaling pathways.

    abstract：:We applied reverse phase protein microarrays technology to map signal pathway interactions in a discovery set of 34 soft tissue sarcoma (STS) bone metastases compared to healthy bone. Proteins associated with matrix remodeling (MMP), adhesion (FAK Y576/577, Syndecan-1), and growth/survival (IGF1R Y1135/1136, PI3K, EGF...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-013-9605-6

    authors： Conti A,Espina V,Chiechi A,Magagnoli G,Novello C,Pazzaglia L,Quattrini I,Picci P,Liotta LA,Benassi MS

    更新日期：2014-01-01 00:00:00

  • Expression and functional analysis of the WAP four disulfide core domain 1 gene in human melanoma.

    abstract：:The exact cellular and molecular mechanisms involved in melanoma tumorigenesis remain obscure. Previous gene expression profiling analyses performed upon NHEM and human melanoma samples identified WFDC1 as one of the most frequently down-regulated genes. Here we further showed that NHEM readily express WFDC1 but expre...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-009-9273-8

    authors： Liu S,Howell P,Ren S,Fodstad O,Zhang G,Samant R,Shevde L,Xi Y,Pannell LK,Riker AI

    更新日期：2009-01-01 00:00:00