Alterations in Cx43 and OB-cadherin affect breast cancer cell metastatic potential.


:Emerging evidence suggests that gap junctional intercellular communication (GJIC) and expression of connexins (Cx) contribute to the metastatic potential of breast cancer cells. To more directly address this, an aggressive bone metastasis breast cancer cell line, MDA-MET (MET), was stably transfected with human Cx43 cDNA (MET/Cx43(+)). Focusing on clone 28 of MET/Cx43(+), we demonstrated that GJIC, Cx43 protein and Cx43 mRNA were significantly increased in MET/Cx43(+) cells relative to MET, the plasmid control for the Cx43 transfectants (MET/HY) and a metastatic breast cancer cell that is less metastatic to bone than MET, MDA-MB-231. Cx26 mRNA was also increased in MET/Cx43(+ )clone 28 cells while mRNA for Cx32, Cx37, Cx40 and Cx45 were not detected in any of the breast cancer cell lines examined. MET/Cx43(+ )clone 28 invasiveness was decreased by 33% relative to MET/HY, while their ability to migrate was unchanged. The ability of MET/Cx43(+ )clone 28 cells to adhere to hFOB and HUV-EC-C cells was decreased approximately 30% and 70%, respectively, relative to MET and MET/HY. E-cadherin and N-cadherin proteins were not detected in MET, MDA-MB-231, MET/Cx43(+ )clone 28 and MET/HY cells. However, OB-cadherin protein levels were decreased approximately 43% in MET/Cx43(+ )clone 28 relative to MET/HY cells. These findings suggest that GJIC and Cx43 expression contribute to breast cancer cell adhesion and migration, possibly through a mechanism involving OB-cadherin, and these changes in turn regulate the metastatic potential of breast cancer cells, especially to bone.


Clin Exp Metastasis


Li Z,Zhou Z,Donahue HJ




Has Abstract


2008-01-01 00:00:00












  • Scatter factor influences the formation of prostate epithelial cell colonies on bone marrow stroma in vitro.

    abstract::Prostate cancer metastases form selectively in the bone marrow. Previously we demonstrated motility was important for the formation of primary prostatic epithelial cell colonies in bone marrow stroma (BMS) co-culture. In this study we looked at the influence of motility factors on the colony formation of epithelial ce...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Lang SH,Clarke NW,George NJ,Testa NG

    更新日期:1999-06-01 00:00:00

  • Preoperative sentinel lymph node mapping of the prostate using PET/CT fusion imaging and Ga-68-labeled tilmanocept in an animal model.

    abstract::Sentinel lymph node (SLN) identification with preoperative image guidance may improve pathological staging of prostate cancer by identifying nodes outside the standard template of dissection. Four anesthetized male dogs received an intra-prostatic injection of Ga-68-labeled tilmanocept. Every 20 min the pelvic lymph n...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Stroup SP,Kane CJ,Farchshchi-Heydari S,James CM,Davis CH,Wallace AM,Hoh CK,Vera DR

    更新日期:2012-10-01 00:00:00

  • Potentiation of cell invasion and matrix metalloproteinase production by alpha3beta1 integrin-mediated adhesion of gastric carcinoma cells to laminin-5.

    abstract::We previously reported that the adhesion of gastric carcinoma cells to the peritoneum mediated by the alpha3beta1 integrin-laminin interaction is a key step in the initial process of peritoneal metastatic dissemination. Carcinoma cells subsequently invade through the intercellular gaps of mesothelial linings. In this ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Saito Y,Sekine W,Sano R,Komatsu S,Mizuno H,Katabami K,Shimada K,Oku T,Tsuji T

    更新日期:2010-04-01 00:00:00

  • Inhibition of lung colonization of mouse colon 26 adenocarcinoma by recombinant mouse interferon beta through a modification of platelet function.

    abstract::Recombinant murine interferon beta (MuIFN-beta) given i.v. efficiently inhibited both pulmonary arrest and formation of lung colonies of NL-17, a highly metastatic variant of mouse colon adenocarcinoma 26. NL-17 was rather resistant to MuIFN-beta in vitro and was highly resistant to natural killer cells of mice even t...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Tsuruo T,Saito H,Watanabe M,Sugimoto Y,Yamori T,Oh-Hara T

    更新日期:1990-03-01 00:00:00

  • Impact of decompressive laminectomy on the functional outcome of patients with metastatic spinal cord compression and neurological impairment.

    abstract::Metastatic spinal cord compression (MSCC) is a frequent phenomenon in advanced tumor diseases with often severe neurological impairments. Affected patients are often treated by decompressive laminectomy. To assess the impact of this procedure on Karnofsky Performance Index (KPI) and Frankel Grade (FG) at discharge, a ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Younsi A,Riemann L,Scherer M,Unterberg A,Zweckberger K

    更新日期:2020-04-01 00:00:00

  • Improvements in population-based survival of patients presenting with metastatic rectal cancer in the south of the Netherlands, 1992-2008.

    abstract::We analysed population-based treatment and survival data of patients who presented with metastatic rectal cancer. All patients diagnosed with primary synchronous metastatic rectal cancer between 1992 and 2008 in the Eindhoven Cancer Registry area were included. Date of diagnosis was divided into three periods (1992-19...

    journal_title:Clinical & experimental metastasis

    pub_type: 历史文章,杂志文章


    authors: Lemmens VE,de Haan N,Rutten HJ,Martijn H,Loosveld OJ,Roumen RM,Creemers GJ

    更新日期:2011-03-01 00:00:00

  • Dendritic cells recruitment in melanoma metastasis treated by electrochemotherapy.

    abstract::Electrochemotherapy (ECT) is a novel treatment for recurrent or in-transit unresectable melanoma metastases based on the administration of anti-neoplastic drugs followed by cancer cell electroporation. Whether ECT can also induce anti-tumour immunity is unclear. We addressed this issue investigating the presence of de...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Gerlini G,Sestini S,Di Gennaro P,Urso C,Pimpinelli N,Borgognoni L

    更新日期:2013-01-01 00:00:00

  • Involvement of cell-cell adhesion molecules in liver colonization by metastatic murine lymphoma/lymphosarcoma variants.

    abstract::Metastatic variant sublines of the murine RAW117 large cell lymphoma or lymphosarcoma have been established in vitro by sequential cycles of harvesting of liver tumor nodules after intravenous inoculation of tumor cell suspensions into syngeneic BALB/c mice. After five and ten in vivo selections for liver colonization...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: McGuire EJ,Mascali JJ,Grady SR,Nicolson GL

    更新日期:1984-07-01 00:00:00

  • Urokinase-type plasminogen activator receptor in gastric cancer: tissue expression and prognostic role.

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    journal_title:Clinical & experimental metastasis

    pub_type: 临床试验,杂志文章


    authors: Plebani M,Herszènyi L,Carraro P,De Paoli M,Roveroni G,Cardin R,Tulassay Z,Naccarato R,Farinati F

    更新日期:1997-07-01 00:00:00

  • Paracrine-mediated osteoclastogenesis by the osteosarcoma MG63 cell line: is RANKL/RANK signalling really important?

    abstract::Although in the past little attention has been paid to the influence of osteosarcoma cells in osteoclast function, recent studies suggest a close relationship between osteosarcoma aggressiveness and osteoclastic activity. The present study addresses the paracrine effects of MG63 cells, a human osteosarcoma-derived cel...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Costa-Rodrigues J,Teixeira CA,Fernandes MH

    更新日期:2011-08-01 00:00:00

  • Enhanced cell surface CD44 variant (v6, v9) expression by osteopontin in breast cancer epithelial cells facilitates tumor cell migration: novel post-transcriptional, post-translational regulation.

    abstract::Osteopontin (OPN) is a glycosylated, secreted phosphoprotein that functions both as a cell attachment and chemotactic factor. Elevated expression of OPN confers enhanced metastatic ability on transformed cells, suggesting that OPN may contribute to the malignant progression of tumors. Migration of mammary carcinoma ce...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Khan SA,Cook AC,Kappil M,Günthert U,Chambers AF,Tuck AB,Denhardt DT

    更新日期:2005-01-01 00:00:00

  • Leading malignant cells initiate collective epithelial cell invasion in a three-dimensional heterotypic tumor spheroid model.

    abstract::Solid tumors consist of genetically and phenotypically diverse subpopulations of cancer cells with unique capacities for growth, differentiation, and invasion. While the molecular and microenvironmental bases for heterogeneity are increasingly appreciated, the outcomes of such intratumor heterogeneity, particularly in...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Carey SP,Starchenko A,McGregor AL,Reinhart-King CA

    更新日期:2013-06-01 00:00:00

  • Adhesion properties, intermediate filaments and malignant behaviour of head and neck squamous cell carcinoma cells in vitro.

    abstract::This study compared phenotype and behaviour of seven head and neck squamous cell carcinoma (HNSCC) cell lines and normal epithelial cells. Indications were found that in HNSCC cells: 1) loss of the cell-cell adhesion molecule E-cadherin was correlated with loss of epithelioid cell morphology but not with loss of cell-...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Tomson AM,Scholma J,Meijer B,Koning JG,de Jong KM,van der Werf M

    更新日期:1996-11-01 00:00:00

  • Multiple effects of transfection with interleukin 2 and/or interferon gamma on the behavior of mouse T lymphoma cells.

    abstract::We have previously reported that transfection of mouse interferon gamma (IFN-gamma) in H-2Kk-positive BW variants (BW-Sp3) abolishes tumorigenicity and reduces metastatic capacity. To further increase the immunogenicity of BW-Sp3 cells, the gene for human interleukin 2 (huIL-2) was transfected in these cancer cells. S...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Geldhof AB,Van Ginderachter J,Vandersteen P,Raes G,De Baetselier P

    更新日期:1998-07-01 00:00:00

  • Inhibition of protein synthesis, pulmonary localization and pulmonary tumour formation by drug-treated tumour cells as a means of predicting their chemosensitivity.

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    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Lai T,Stonebridge BR,Black J,Symes MO

    更新日期:1989-07-01 00:00:00

  • Anti-metastatic properties of RGD-peptidomimetic agents S137 and S247.

    abstract::Integrins expressed on endothelial cells modulate cell migration and survival during angiogenesis. Integrins expressed on carcinoma cells potentiate metastasis by facilitating invasion and movement across blood vessels. We describe the activities of two synthetic low-molecular-weight peptidomimetics of the ligand amin...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Shannon KE,Keene JL,Settle SL,Duffin TD,Nickols MA,Westlin M,Schroeter S,Ruminski PG,Griggs DW

    更新日期:2004-01-01 00:00:00

  • Predictors of prognosis of synchronous brain metastases in small-cell lung cancer patients.

    abstract::Patients with small cell lung cancer (SCLC) are more likely to have synchronous brain metastasis (SBM) at the time of diagnosis than patients with any other extracranial primary malignancy. We sought to identify which factors predicted an increased risk of SBM in SCLC as well as which factors affected the prognosis of...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Reddy SP,Dowell JE,Pan E

    更新日期:2020-08-01 00:00:00

  • Bone microenvironment-mediated resistance of cancer cells to bisphosphonates and impact on bone osteocytes/stem cells.

    abstract::Anti-resorptive bisphosphonates (BPs) have been clinically used to prevent cancer-bone metastasis and cancer-induced bone pathologies despite the fact that the phenotypic response of the cancer-bone interactions to BP exposure is "uncharted territory". This study offers unique insights into the interplay between cance...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Alasmari A,Lin SC,Dibart S,Salih E

    更新日期:2016-08-01 00:00:00

  • Automated CT-based analysis to detect changes in the prevalence of lytic bone metastases from breast cancer.

    abstract::The spinal column is the most frequent site of bone metastasis in patients with breast cancer. It is important to understand how the pattern of vertebral lesions may be affected by the introduction of modern cancer therapies. The purpose of this study was to characterize changes in the radiological appearance of spina...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Skrinskas T,Clemons M,Freedman O,Weller I,Whyne CM

    更新日期:2009-01-01 00:00:00

  • Risk factors and survival outcomes in patients with brain metastases from breast cancer.

    abstract::Development of central nervous system (CNS) metastases in breast cancer (BC) is associated with poor prognosis. The incidence of CNS metastases in metastatic BC is reported to be about 10-16 %, but different subtypes of BC are associated with different risk of developing CNS metastases. We retrospectively analysed the...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Minisini AM,Moroso S,Gerratana L,Giangreco M,Iacono D,Poletto E,Guardascione M,Fontanella C,Fasola G,Puglisi F

    更新日期:2013-12-01 00:00:00

  • Naturally occurring resistance of bone marrow mononuclear and metastatic cancer cells to anticancer agents.

    abstract::Numerous cancer patients fail standard chemotherapy or develop resistance to chemotherapy during the course of treatment. The purpose of this study is to elucidate the overall response of cells obtained from cancer patients and from normal individuals to chemotherapeutic agents. We analysed the chemosensitivity of can...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Richard C,Yau J,Th'ng JP,Duivenvoorden WC

    更新日期:2006-01-01 00:00:00

  • Parathyroid hormone-related protein and ezrin are up-regulated in human lung cancer bone metastases.

    abstract::Lung cancer often metastasizes to bone in patients with advanced disease. Identification of the factors involved in the interactions between lung cancer cells and bone will improve the prevention and treatment of bone metastases. We identified changes in metastasis-related gene expression of human HARA lung squamous c...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Deng X,Tannehill-Gregg SH,Nadella MV,He G,Levine A,Cao Y,Rosol TJ

    更新日期:2007-01-01 00:00:00

  • A high expression level of insulin-like growth factor I receptor is associated with increased expression of transcription factor Sp1 and regional lymph node metastasis of human gastric cancer.

    abstract::Insulin-like growth factor I receptor (IGF-IR) is critical to cell survival and growth and altered IGF-IR expression is found in many human cancers. However, its expression and potential role in gastric cancer development and progression has not been explored. The IGF-IR expression level was determined via immunohisto...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Jiang Y,Wang L,Gong W,Wei D,Le X,Yao J,Ajani J,Abbruzzese JL,Huang S,Xie K

    更新日期:2004-01-01 00:00:00

  • Analyses of the role of endogenous SPARC in mouse models of prostate and breast cancer.

    abstract::Secreted protein, acidic and rich in cysteine (SPARC, also known as osteonectin or BM-40) is a glycoprotein component of the extracellular matrix that has been reported to be involved with a variety of cellular processes. Although SPARC expression levels are frequently altered in a variety of tumor types, the exact im...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Wong SY,Crowley D,Bronson RT,Hynes RO

    更新日期:2008-01-01 00:00:00

  • Differential growth and responsiveness to cancer therapy of tumor cells in different environments.

    abstract::Tumor metastasis often confers poor prognosis for cancer patients due to lack of comprehensive strategy in dealing with cells growing in different environment. Current anticancer therapies have incomplete effectiveness because they were designed assuming metastatic tumors behave similarly in different organs. We hypot...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Alsaggar M,Yao Q,Cai H,Liu D

    更新日期:2016-02-01 00:00:00

  • Inhibition of liver tumor cell colonization in two animal tumor models by lectin blocking with D-galactose or arabinogalactan.

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    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Beuth J,Ko HL,Schirrmacher V,Uhlenbruck G,Pulverer G

    更新日期:1988-03-01 00:00:00

  • Comparative study on Lewis lung tumour lines with 'low' and 'high' metastatic capacity. III. Glycosaminoglycan synthesis, transport and degradation in cell lines.

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    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Pogány G,Moczar E,Jeney A,Timár J,Timár F,Ditrói K,Lapis K

    更新日期:1989-11-01 00:00:00

  • SCUBE3 regulation of early lung cancer angiogenesis and metastatic progression.

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    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Chou CH,Cheng YF,Siow TY,Kumar A,Peck K,Chang C

    更新日期:2013-08-01 00:00:00

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    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Suarez-Cuervo C,Merrell MA,Watson L,Harris KW,Rosenthal EL,Väänänen HK,Selander KS

    更新日期:2004-01-01 00:00:00

  • Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis.

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    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Boiy R,Steenbrugge J,Van Deun J,Hendrix A,Meyer E,De Wever O

    更新日期:2018-12-01 00:00:00