Abstract：
:Emerging evidence suggests that gap junctional intercellular communication (GJIC) and expression of connexins (Cx) contribute to the metastatic potential of breast cancer cells. To more directly address this, an aggressive bone metastasis breast cancer cell line, MDA-MET (MET), was stably transfected with human Cx43 cDNA (MET/Cx43(+)). Focusing on clone 28 of MET/Cx43(+), we demonstrated that GJIC, Cx43 protein and Cx43 mRNA were significantly increased in MET/Cx43(+) cells relative to MET, the plasmid control for the Cx43 transfectants (MET/HY) and a metastatic breast cancer cell that is less metastatic to bone than MET, MDA-MB-231. Cx26 mRNA was also increased in MET/Cx43(+ )clone 28 cells while mRNA for Cx32, Cx37, Cx40 and Cx45 were not detected in any of the breast cancer cell lines examined. MET/Cx43(+ )clone 28 invasiveness was decreased by 33% relative to MET/HY, while their ability to migrate was unchanged. The ability of MET/Cx43(+ )clone 28 cells to adhere to hFOB and HUV-EC-C cells was decreased approximately 30% and 70%, respectively, relative to MET and MET/HY. E-cadherin and N-cadherin proteins were not detected in MET, MDA-MB-231, MET/Cx43(+ )clone 28 and MET/HY cells. However, OB-cadherin protein levels were decreased approximately 43% in MET/Cx43(+ )clone 28 relative to MET/HY cells. These findings suggest that GJIC and Cx43 expression contribute to breast cancer cell adhesion and migration, possibly through a mechanism involving OB-cadherin, and these changes in turn regulate the metastatic potential of breast cancer cells, especially to bone.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Li Z,Zhou Z,Donahue HJdoi
10.1007/s10585-007-9140-4subject
Has Abstractpub_date
2008-01-01 00:00:00pages
265-72issue
3eissn
0262-0898issn
1573-7276journal_volume
25pub_type
杂志文章abstract：:We applied reverse phase protein microarrays technology to map signal pathway interactions in a discovery set of 34 soft tissue sarcoma (STS) bone metastases compared to healthy bone. Proteins associated with matrix remodeling (MMP), adhesion (FAK Y576/577, Syndecan-1), and growth/survival (IGF1R Y1135/1136, PI3K, EGF...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9605-6
更新日期：2014-01-01 00:00:00
abstract：:MicroRNAs are a class of ≈22-nt noncoding single-strand RNAs regulating gene expression postscriptionally. Metastasis caused poor prognosis in colorectal cancer patients and half of the patients developed metastatic lesions when admission. Here we investigated the possible roles of microRNAs in regulating metastasis i...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9463-7
更新日期：2012-06-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1013849123736
更新日期：2002-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006783330996
更新日期：1999-01-01 00:00:00
abstract：:Numerous cancer patients fail standard chemotherapy or develop resistance to chemotherapy during the course of treatment. The purpose of this study is to elucidate the overall response of cells obtained from cancer patients and from normal individuals to chemotherapeutic agents. We analysed the chemosensitivity of can...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-006-9034-x
更新日期：2006-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006757525190
更新日期：1999-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-009-9299-y
更新日期：2010-01-01 00:00:00
abstract：:Emerging data in myeloma and other cancers indicates that heparan sulfate proteoglycans promote tumor progression by enhancing their growth and metastasis. By acting as key regulators of cell signaling via their interactions with multiple growth and angiogenic factors, heparan sulfates mediate a shift in the microenvi...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-007-9125-3
更新日期：2008-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006715221088
更新日期：2000-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9783-0
更新日期：2016-04-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9598-1
更新日期：2013-12-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-018-9892-z
更新日期：2018-08-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1023/a:1022550721404
更新日期：2003-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006696002497
更新日期：1999-06-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01753663
更新日期：1989-07-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-004-2696-3
更新日期：2004-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9565-x
更新日期：2013-06-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/b:clin.0000046140.19131.19
更新日期：2004-01-01 00:00:00
abstract：:The effect of thioglycollate-elicited macrophages (TG-M phi) on natural killer (NK)-cell activity and metastases formation in mice was investigated. Intravenously (i.v.) inoculated TG-M phi inhibited spleen NK activity of normal mice and abrogated polyinosinic: polycytidylic (poly I:C) induced augmentation of NK cell ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01758960
更新日期：1985-04-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00058169
更新日期：1992-09-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01753893
更新日期：1994-05-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01753676
更新日期：1989-11-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01784842
更新日期：1988-03-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-005-1198-2
更新日期：2004-01-01 00:00:00
abstract：:Recently the concept that gap junctions play a role in cancer cell metastasis has emerged. However, the mechanism by which this might occur is unknown. To examine this issue a metastatic breast cancer cell line, MDA-MB-435, was stably transfected with human Cx43 cDNA. Four clones of 435 transfectants (435/Cx43(+) c1, ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-008-9208-9
更新日期：2008-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-004-3503-x
更新日期：2004-01-01 00:00:00
abstract：:Despite an initial response to antihormonal therapies, the development of resistance will occur in a significant number of breast cancer patients. The mechanisms that underlie acquired resistance are not yet clear. Using a previously established in vitro cell model of tamoxifen resistance in MCF7 cells, shown to displ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/b:clin.0000037697.76011.1d
更新日期：2004-01-01 00:00:00
abstract：:The lungs of BR6 breeding female mice which had developed spontaneous mammary tumours, were examined histologically for the presence of metastases. Metastatic nodules, or tumour cell emboli within blood vessels, were found in the lungs of 3 out of 42 (7 per cent) mice with pregnancy-dependent tumours, and in 33 out of...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00736406
更新日期：1983-07-01 00:00:00
abstract：:Vascularization of solid tumors is thought to occur by sprouting or intussusceptive angiogenesis, co-option of existing vessels, and vasculogenic mimicry after the onset of tumor hypoxia, when the tumor radius exceeds the oxygen diffusion distance. In contrast, here we show that individual endothelial cells that are i...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-009-9257-8
更新日期：2009-01-01 00:00:00
abstract：:Previous studies have shown that thrombospondin (TSP) is an adhesion factor for some human tumor cells. The previous studies have shown further that tumor cells which utilize TSP as an adhesion factor also synthesize it. This study continues the effort to understand how TSP production and expression are regulated in h...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01753679
更新日期：1989-05-01 00:00:00
