Abstract：
:We applied reverse phase protein microarrays technology to map signal pathway interactions in a discovery set of 34 soft tissue sarcoma (STS) bone metastases compared to healthy bone. Proteins associated with matrix remodeling (MMP), adhesion (FAK Y576/577, Syndecan-1), and growth/survival (IGF1R Y1135/1136, PI3K, EGFR) were elevated in metastasis compared to normal bone. Linkage between Syndecan-1, FAK Y576/577, Shc Y317, and EGFR, IGF Y1135/1136, PI3K/AKT was a prominent feature of STS bone metastasis. Elevated linkage between RANKL and 4EBP1 T37/46, EGFR, IGF1R Y1135/1136, Src Y41, Shc Y317, PI3Kp110γ was associated with short survival. Finally, we tested the hypothesis that signal pathway proteins augmented in the STS bone metastasis may provide clues to understand the subset of primary STS that metastasize. The most representative molecules identified in the discovery set were validated on an independent series of 82 primary STS by immunohistochemistry applied to a tissue microarray. The goal was to correlate the molecular profile in the primary tumors with a higher likelihood of metastasis. Elevation of activated kinase substrate endpoints IRS1 S612, 4EBP1 T37/46, FAK Y576/577 and loss of Fibronectin, were associated with a higher likelihood of metastases. These data indicate that the linkage between matrix remodeling, adhesion, and growth signaling may drive STS metastasis and can be the basis for prognostic and therapeutic strategies.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Conti A,Espina V,Chiechi A,Magagnoli G,Novello C,Pazzaglia L,Quattrini I,Picci P,Liotta LA,Benassi MSdoi
10.1007/s10585-013-9605-6subject
Has Abstractpub_date
2014-01-01 00:00:00pages
15-24issue
1eissn
0262-0898issn
1573-7276journal_volume
31pub_type
杂志文章abstract：:In order to study the usefulness of treatment with vinblastine (VLB) in the prevention of cancer metastasis in squamous cell lung cancer, 50 patients with locoregional disease were randomized to receive either locoregional RT alone (group A) or a weekly intravenous bolus injection of VLB (6 mg/m2) concurrently with an...
journal_title：Clinical & experimental metastasis
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF01580405
更新日期：1988-01-01 00:00:00
abstract：:Intravenous infusions of perfluorochemical emulsions, combined with administration of inspired oxygen or carbogen have been found to improve tumor oxygenation and increase the response of solid tumors in animals to radiotherapy. Fluosol-DA 20 per cent, the only perfluorochemical emulsion currently approved for testing...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00053472
更新日期：1986-01-01 00:00:00
abstract：:Use of heparin derivatives in several cancer types revealed that anticoagulant therapies have a beneficiary side effect: delay of tumor progression. Since there are no data on human melanoma, we have analyzed the effect of heparins in preclinical models. Neither unfractionated heparin (UFH), nor its low molecular weig...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-005-3859-6
更新日期：2005-01-01 00:00:00
abstract：:Proliferation capacity and MHC class I antigen expression of two Lewis lung carcinoma (3LL) metastatic variants (C87, BC215) grown under defined experimental conditions (serum-free defined medium or 10 per cent serum) have been studied following exposure to MoAb 135-13C which recognizes on these cells a tumor surface ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF02057180
更新日期：1989-01-01 00:00:00
abstract：:Metastatic castrate resistant prostate cancer (mCRPC) is responsible for the majority of prostate cancer deaths with the median survival after diagnosis being 2 years. The metastatic lesions often arise in the skeleton, and current treatment options are primarily palliative. Using guidelines set forth by the National ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-014-9674-1
更新日期：2014-12-01 00:00:00
abstract：:Metastatic spinal cord compression (MSCC) is a frequent phenomenon in advanced tumor diseases with often severe neurological impairments. Affected patients are often treated by decompressive laminectomy. To assess the impact of this procedure on Karnofsky Performance Index (KPI) and Frankel Grade (FG) at discharge, a ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-019-10016-z
更新日期：2020-04-01 00:00:00
abstract：:The expression of both 92- and 72-kDa gelatinases has been studied in 20 samples of human breast carcinoma by the technique of gelatin zymography. This technique allowed the relative amount of each gelatinase to be determined in small samples of tissue (< 10 mg). More importantly, active and latent forms of the two ge...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00114976
更新日期：1993-03-01 00:00:00
abstract：:Breast cancer has a predilection for metastasis to the bone marrow. The preprotachykinin-I (PPT-I) gene has a central role in the early migration of breast cancer cells into the bone marrow, making this organ a latent repository of the cancer cells. This study investigated whether the invasive and metastatic potential...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-006-9001-6
更新日期：2005-01-01 00:00:00
abstract：:For more effective oncological management of disseminated colorectal cancer, therapies must be devised that target the different individual stages of metastasis development. Recent work showed that integrin subunits alpha2, alpha6 and beta4 are involved in the colorectal cancer cell extravasation process. By means of ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-009-9276-5
更新日期：2009-01-01 00:00:00
abstract：:Several lines of evidence indicate that sialosyl Le(a), tumor-associated carbohydrate antigen present on human colon carcinoma cells, is involved in formation of metastases. To study the role of this carbohydrate structure in development of metastases, we have used the clone of human colon carcinoma CX-1 cells transfe...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006502009682
更新日期：1998-11-01 00:00:00
abstract：:Cancer stem cells drive the metastatic cascade by undergoing epithelial to mesenchymal transition (EMT) and again mesenchymal to epithelial transition (MET). Using multiple breast cancer cell lines including cigarette smoke induced breast cancer cells and tumor derived primary cells from patient sample; we developed a...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9809-7
更新日期：2016-10-01 00:00:00
abstract：BACKGROUND:One of the major clinical problems in breast cancer detection is the relatively high incidence of occult lymph node metastases undetectable by standard procedures. Since the ascertainment of breast cancer stage determines the following treatment, such a "hypo-diagnosis" leads to inadequate therapy, and hence...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9135-1
更新日期：2008-01-01 00:00:00
abstract：:Tumor metastases are extremely rare in striated muscles. This is surprising given the fact that this tissue constitutes 60% of body weight. The present study focuses on small molecules produced and secreted by muscle cells which possess anti-cancer activity in vivo. Recently we have shown that a low molecular weight f...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006649617918
更新日期：1999-01-01 00:00:00
abstract：:The spinal column is the most frequent site of bone metastasis in patients with breast cancer. It is important to understand how the pattern of vertebral lesions may be affected by the introduction of modern cancer therapies. The purpose of this study was to characterize changes in the radiological appearance of spina...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-008-9219-6
更新日期：2009-01-01 00:00:00
abstract：:Osteopontin (OPN) is a glycosylated, secreted phosphoprotein that functions both as a cell attachment and chemotactic factor. Elevated expression of OPN confers enhanced metastatic ability on transformed cells, suggesting that OPN may contribute to the malignant progression of tumors. Migration of mammary carcinoma ce...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-006-9007-0
更新日期：2005-01-01 00:00:00
abstract：:MicroRNAs have been identified as key players in the development and progression of osteosarcoma, which is the most common primary malignancy of bone. Sequencing-based miR-omic and quantitative real-time PCR analyses suggested that the expression of miR-193a-3p and miR-193a-5p was decreased by DNA methylation at their...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9783-0
更新日期：2016-04-01 00:00:00
abstract：:Prostate cancer metastases form selectively in the bone marrow. Previously we demonstrated motility was important for the formation of primary prostatic epithelial cell colonies in bone marrow stroma (BMS) co-culture. In this study we looked at the influence of motility factors on the colony formation of epithelial ce...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006696002497
更新日期：1999-06-01 00:00:00
abstract：:Breast cancer metastasizes to bone with high frequency and incidence. However, studies of breast cancer metastasis to bone have been limited by two factors. First, the number of models that colonize bone are limited. Second, detection of bone metastases is too insensitive or too laborious for routine, large-scale stud...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1024062911144
更新日期：2003-01-01 00:00:00
abstract：:Lymphangiogenesis plays a crucial role in promoting cancer metastasis to sentinel lymph nodes (LNs) and beyond. Increasing data have shown that simvastatin, a cholesterol-lowering medication for the prevention of cardiovascular diseases, is involved in tumor growth and dissemination, and endothelial functions. This st...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-018-9940-8
更新日期：2018-12-01 00:00:00
abstract：:Patients with small cell lung cancer (SCLC) are more likely to have synchronous brain metastasis (SBM) at the time of diagnosis than patients with any other extracranial primary malignancy. We sought to identify which factors predicted an increased risk of SBM in SCLC as well as which factors affected the prognosis of...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-020-10040-4
更新日期：2020-08-01 00:00:00
abstract：:This study examined the relationship between the expression of E-cadherin or beta-catenin in murine adenocarcinomas and their hematogenous metastatic propensity, assessed by both spontaneous and artificial lung metastasis. Seven different carcinomas, syngeneic to C3Hf/Kam mice were used: 4 mammary carcinomas (MCa-4, M...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006670918848
更新日期：1999-03-01 00:00:00
abstract：:Numerous cancer patients fail standard chemotherapy or develop resistance to chemotherapy during the course of treatment. The purpose of this study is to elucidate the overall response of cells obtained from cancer patients and from normal individuals to chemotherapeutic agents. We analysed the chemosensitivity of can...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-006-9034-x
更新日期：2006-01-01 00:00:00
abstract：:We studied the influence of 10 synthetic glycoamine analogs on colony formation in 0.3 and 0.9% agarose by metastatic human breast carcinoma (MDA-MB-435) and melanoma (TXM-13) cells. Nine synthetic analogs significantly inhibited the colony formation in 0.9% agarose of MDA-MB-435 human breast carcinoma cells; five com...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00053899
更新日期：1996-05-01 00:00:00
abstract：:Secreted protein, acidic and rich in cysteine (SPARC, also known as osteonectin or BM-40) is a glycoprotein component of the extracellular matrix that has been reported to be involved with a variety of cellular processes. Although SPARC expression levels are frequently altered in a variety of tumor types, the exact im...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9126-2
更新日期：2008-01-01 00:00:00
abstract：:Metastatic variant sublines of the murine RAW117 large cell lymphoma or lymphosarcoma have been established in vitro by sequential cycles of harvesting of liver tumor nodules after intravenous inoculation of tumor cell suspensions into syngeneic BALB/c mice. After five and ten in vivo selections for liver colonization...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00132928
更新日期：1984-07-01 00:00:00
abstract：:Immunotherapy by vaccination represents a novel method for treatment of cancer. In this regard, vaccines with the broadest possible menu of relevant antigens stand the greatest chance of success. Tissue vaccines are composed of material harvested directly from tumors and contain not only antigens associated with neopl...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-008-9213-z
更新日期：2008-01-01 00:00:00
abstract：:We previously isolated thymosin beta15 from highly metastatic Dunning rat prostatic carcinoma cells. Immunohistochemical study of human prostate cancer specimens revealed a general correlation between Gleason grade and thymosin beta15 expression, with high-grade (more malignant) tumors showing increased staining compa...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006540824969
更新日期：1998-04-01 00:00:00
abstract：:A number of papers have been published on the clinical correlation of the expression of the 67 kDa laminin binding protein (LBP) with the metastatic potential of solid tumors. Both mRNA and protein expression levels have been reported, but both the relationship between them and the molecular nature of the 67 kDa surfa...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00121912
更新日期：1995-09-01 00:00:00
abstract：:Tumor cell adhesion to the extracellular matrix (ECM) is closely linked with tumor cell invasion and metastasis. In this study, we demonstrate that low levels of adriamycin, a widely used anticancer drug, can inhibit the invasion of highly metastatic K1735-M2 mouse melanoma cells in vitro through a reconstituted basem...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00880070
更新日期：1993-01-01 00:00:00
abstract：:Treating metastatic osteosarcoma (OS) remains a challenge in oncology. Current treatment strategies target the primary tumour rather than metastases and have a limited efficacy in the treatment of metastatic disease. Metastatic cells have specific features that render them less sensitive to therapy and targeting these...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-011-9384-x
更新日期：2011-06-01 00:00:00
