Abstract：
:Breast cancer patients with advanced disease exhibit bone metastases, leading to the formation of osteolytic lesions for which the only currently available treatments are palliative. Here, we describe how we refined a mouse model of human breast cancer metastasis into bone, characterized its transcriptome and demonstrated its clinical relevance. Cells were selected from bone metastases caused by MDA-MB-231 cells after several in vivo passages, and engineered to express luciferase. Whole body bioluminescence live imaging indicated that the selected isogenic B02 clone was unique in its ability to form rapidly growing osteolytic bone metastases. B02 cells were detected as early as 10 days after tail vein injection, as opposed to 1 month after cardiac injection in other haematogenous models. Whole transcriptomic analysis identified 114 upregulated and 247 downregulated genes in B02 cells compared to the parental cells, several of which represent novel targets. In addition, there was a 50% overlap between the B02 signature and a recently described signature obtained from human breast cancer bone metastases. Consistent with the plasticity of an aggressive metastatic variant, 10% of the regulated genes are involved in proliferation, migration, invasion and angiogenesis. Strikingly, B02 cells also express osteoblast-specific genes, thus mimicking a process referred to as osteomimicry in the clinic. The B02 cells "human bone metastatic signature", the expression of bone-specific genes, as well as the live imaging of this convenient model highlight its clinical relevance and usefulness during drug development.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Garcia T,Jackson A,Bachelier R,Clément-Lacroix P,Baron R,Clézardin P,Pujuguet Pdoi
10.1007/s10585-007-9099-1subject
Has Abstractpub_date
2008-01-01 00:00:00pages
33-42issue
1eissn
0262-0898issn
1573-7276journal_volume
25pub_type
杂志文章abstract：:Vascularization of solid tumors is thought to occur by sprouting or intussusceptive angiogenesis, co-option of existing vessels, and vasculogenic mimicry after the onset of tumor hypoxia, when the tumor radius exceeds the oxygen diffusion distance. In contrast, here we show that individual endothelial cells that are i...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-009-9257-8
更新日期：2009-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9527-8
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1011085820250
更新日期：2000-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9547-4
更新日期：2013-04-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-009-9276-5
更新日期：2009-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01580402
更新日期：1988-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01753685
更新日期：1989-05-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-008-9213-z
更新日期：2008-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-018-9944-4
更新日期：2018-12-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/b:clin.0000046140.19131.19
更新日期：2004-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-006-9007-0
更新日期：2005-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9452-x
更新日期：2012-04-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9624-3
更新日期：2014-02-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-005-7889-x
更新日期：2005-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00736406
更新日期：1983-07-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-005-1198-2
更新日期：2004-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-020-10040-4
更新日期：2020-08-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-019-09960-7
更新日期：2019-04-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01784377
更新日期：1988-11-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,多中心研究
doi：10.1007/s10585-015-9763-9
更新日期：2016-02-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006542301794
更新日期：1998-08-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9605-6
更新日期：2014-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-018-9892-z
更新日期：2018-08-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1013849123736
更新日期：2002-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1018429600437
更新日期：1997-05-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00132927
更新日期：1984-07-01 00:00:00
abstract：:Cancer cells with the surface marker profile CD44+/CD24- have previously been described to possess cancer stem cell-like properties. This manuscript evaluates those properties in ovarian cancer cell lines. The proportion of CD44+/CD24- cells corresponded to the clinical aggressiveness of each ovarian cancer cell line ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9482-4
更新日期：2012-12-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-014-9689-7
更新日期：2015-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1011884807746
更新日期：2000-01-01 00:00:00
abstract：:Signal peptide-CUB-EGF-like domain-containing protein 3 (SCUBE3) is strongly expressed in extremely invasive lung carcinoma. We showed in our previous study that SCUBE3 triggers the transforming growth factor-β pathway and subsequently promotes tumor angiogenesis and the epithelial-mesenchymal transition (EMT). Howeve...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9575-8
更新日期：2013-08-01 00:00:00
