Abstract：
:The mechanisms that control the insidiously invasive nature of malignant gliomas are poorly understood, and their study would be facilitated by an in vivo model that is easy to manipulate and inexpensive. The developing chick embryo brain was assessed as a new xenograft model for the production, growth, and study of human and rat glioma cell lines. Three established glioma lines (U-87 MG, C6, and 9L) were injected into chick embryo brain ventricles on embryonic day (E) 5 and brains were examined after several days to two weeks after injection. All glioma lines survived, produced vascularized intraventricular tumors, and invaded the brain in a manner similar to that in rodents. Rat C6 glioma cells spread along vasculature and also invaded the neural tissue. Human U-87 glioma cells migrated along vasculature and exhibited slight invasion of neural tissue. Rat 9L gliosarcoma cells were highly motile, but migrated only along the vasculature. A derivative of 9L cells that stably expressed the cell surface adhesion molecule NgCAM/L1 was produced and also injected into chick embryo brain ventricles to see if this protein could facilitate tumor cell migration away from the vasculature into areas such as axonal tracts. 9L/NgCAM cells, however, did not migrate away from the vasculature and, thus, this protein alone cannot be responsible for diffuse invasiveness of some gliomas. 9L/NgCAM cell motility was assessed in vitro using sophisticated time-lapse microscopy and quantitative analysis, and was significantly altered compared to parental 9L cells. These studies demonstrate that the chick embryo brain is a successful and novel xenograft model for mammalian gliomas and demonstrate the potential usefulness of this new model for studying glioma tumor cell growth, vascularization, and invasiveness.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Cretu A,Fotos JS,Little BW,Galileo DSdoi
10.1007/s10585-005-7889-xsubject
Has Abstractpub_date
2005-01-01 00:00:00pages
225-36issue
3eissn
0262-0898issn
1573-7276journal_volume
22pub_type
杂志文章abstract：:Signal peptide-CUB-EGF-like domain-containing protein 3 (SCUBE3) is strongly expressed in extremely invasive lung carcinoma. We showed in our previous study that SCUBE3 triggers the transforming growth factor-β pathway and subsequently promotes tumor angiogenesis and the epithelial-mesenchymal transition (EMT). Howeve...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9575-8
更新日期：2013-08-01 00:00:00
abstract：:The chemokine CXCL12/SDF-1 and its receptors CXCR4 and CXCR7 play a major role in tumor invasion, proliferation and metastasis. Since both receptors are overexpressed on distinct tumor cells and on the tumor vasculature, we evaluated their potential as targets for detection of cancers by molecular imaging. We synthesi...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-011-9403-y
更新日期：2011-12-01 00:00:00
abstract：:Elevated osteopontin (OPN) transcription often correlates with increased metastatic potential of transformed cells, and in several model systems OPN--whether produced by the tumor cells or by stromal cells - has been shown to enhance metastatic ability. Sequence elements in the OPN promoter have been identified on the...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1023/a:1022550721404
更新日期：2003-01-01 00:00:00
abstract：:Circulating microRNAs have been described as head and neck cancer biomarkers in multiple anatomical subsites including the oral cavity, nasopharynx, larynx, salivary glands and the skin. While there is an expanding volume of published literature showing the significance of individual or panels of microRNAs, the clinic...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-019-09961-6
更新日期：2019-06-01 00:00:00
abstract：:It is recognised that stromal cells determine cancer progression. We have previously shown that active TGFbeta produced by rat colon carcinoma cells modulated NO production in rat endothelial cells. To elucidate the role of TGFbeta and NO in the mechanisms of interaction of colon carcinoma cells with stromal cells and...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-005-0431-3
更新日期：2005-01-01 00:00:00
abstract：:We have previously isolated a series of MCF-7 human breast cancer cell variants which no longer require estrogen-supplementation for tumor growth in nude mice (Clarke et al. Proc Natl Acad Sci USA 86: 3649-3653, 1989). We now report that these hormone-independent and hormone-responsive variants (MIII, MCF7/LCC1) can i...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00880062
更新日期：1993-01-01 00:00:00
abstract：:Electrochemotherapy (ECT) is a novel treatment for recurrent or in-transit unresectable melanoma metastases based on the administration of anti-neoplastic drugs followed by cancer cell electroporation. Whether ECT can also induce anti-tumour immunity is unclear. We addressed this issue investigating the presence of de...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9505-1
更新日期：2013-01-01 00:00:00
abstract：:MACC1 (metastasis associated in colon cancer 1) is a key driver that induces metastasis in colon cancer. However, the mechanisms by which MACC1 expression is transcriptionally regulated and the factors enriched at the MACC1 promoter remain largely unknown. The binding of proteins to specific DNA sites in the genome is...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-020-10045-z
更新日期：2020-08-01 00:00:00
abstract：:Insulin-like growth factor I receptor (IGF-IR) is critical to cell survival and growth and altered IGF-IR expression is found in many human cancers. However, its expression and potential role in gastric cancer development and progression has not been explored. The IGF-IR expression level was determined via immunohisto...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-005-1198-2
更新日期：2004-01-01 00:00:00
abstract：:Development of central nervous system (CNS) metastases in breast cancer (BC) is associated with poor prognosis. The incidence of CNS metastases in metastatic BC is reported to be about 10-16 %, but different subtypes of BC are associated with different risk of developing CNS metastases. We retrospectively analysed the...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9594-5
更新日期：2013-12-01 00:00:00
abstract：:Lung cancer often metastasizes to bone in patients with advanced disease. Identification of the factors involved in the interactions between lung cancer cells and bone will improve the prevention and treatment of bone metastases. We identified changes in metastasis-related gene expression of human HARA lung squamous c...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9059-9
更新日期：2007-01-01 00:00:00
abstract：:MicroRNAs have been identified as key players in the development and progression of osteosarcoma, which is the most common primary malignancy of bone. Sequencing-based miR-omic and quantitative real-time PCR analyses suggested that the expression of miR-193a-3p and miR-193a-5p was decreased by DNA methylation at their...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9783-0
更新日期：2016-04-01 00:00:00
abstract：:Platelet-derived growth factor BB (PDGF BB) and the PDGF receptor beta are expressed on mesothelioma cells, but their biological function has not yet been defined. In the present study we used Boyden chambers fitted with filters coated with the adhesive matrix proteins fibronectin, laminin, collagen type IV or the non...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006542301794
更新日期：1998-08-01 00:00:00
abstract：:Immunotherapy by vaccination represents a novel method for treatment of cancer. In this regard, vaccines with the broadest possible menu of relevant antigens stand the greatest chance of success. Tissue vaccines are composed of material harvested directly from tumors and contain not only antigens associated with neopl...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-008-9213-z
更新日期：2008-01-01 00:00:00
abstract：:To aid in therapy selection for patients with spinal bone metastases (SBM), predictive models have been developed. These models consider SBM from breast cancer a positive predictive factor, but do not take phenotypes based on estrogen (ER), progesterone (PR) and human epidermal growth factor 2 (HER2) receptors into ac...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,多中心研究
doi：10.1007/s10585-014-9685-y
更新日期：2015-01-01 00:00:00
abstract：:Experimental hepatic metastasis of colorectal tumors is frequently studied by local intrahepatic tumor cell implantation. However, although a variety of factors of the implantation procedure may markedly influence tumor growth characteristics, standards are not defined yet. Herein, we studied the effect of different m...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-004-2696-3
更新日期：2004-01-01 00:00:00
abstract：:Anti-resorptive bisphosphonates (BPs) have been clinically used to prevent cancer-bone metastasis and cancer-induced bone pathologies despite the fact that the phenotypic response of the cancer-bone interactions to BP exposure is "uncharted territory". This study offers unique insights into the interplay between cance...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9798-6
更新日期：2016-08-01 00:00:00
abstract：:To provide an investigative tool for the study of osteosarcoma (OSA) biology we have developed a syngeneic (balb/c) murine model of OSA, using cell lines derived from a spontaneously occurring murine OSA (Schmidt et al. Differentiation 1988; 39: 151-60). This model is characterized by orthotopic primary tumor growth, ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006767007547
更新日期：2000-01-01 00:00:00
abstract：:Extrathyroidal extension (ETE) of papillary thyroid carcinoma (PTC) is common and clinical presentation can vary from minimal to extensive locoregional involvement. Although PTC is generally considered the most benign among all thyroid carcinomas, it may present with local invasion with poor prognosis. Our retrospecti...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9800-3
更新日期：2016-08-01 00:00:00
abstract：:A murine mammary carcinoma, which had a high potential for metastasis to the lungs, was established in culture, and from the parent line several clonally derived variants were isolated, showing different characteristics including metastatic potential. C1, a high metastatic clone, and C2, a low one, were selected for f...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01784377
更新日期：1988-11-01 00:00:00
abstract：:The concurrent administration of Metrazol (60 mg/kg, i.v.) to anaesthetized rats enhances the cerebral penetration of the anticancer agent razoxane. Such an enhancement leads to an increase in the therapeutic efficacy of razoxane against intracerebrally sequestered L1210 leukaemia cells in mice. The combination of Met...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00132306
更新日期：1984-01-01 00:00:00
abstract：:Considering recent findings that cyclooxygensase-2 (COX-2) is involved in the progression of colorectal carcinoma (CRC), the role of COX-2 in promoting invasion and angiogenesis was investigated by evaluating the relationship of COX-2 expression to various clinicopathological variables, including plasminogen activatin...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1020392309715
更新日期：2002-01-01 00:00:00
abstract：:To evaluate the local control (LC), progression free survival (PFS), out-field PFS, overall survival (OS), toxicity and failure predictors of SRT in a series of various sites oligometastatic CRC patients. Patients with oligometastatic CRC disease were analyzed retrospectively. The SRT prescribed dose was dependent on ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-019-09976-z
更新日期：2019-08-01 00:00:00
abstract：:Tumour endothelial markers (TEMs) are a newly discovered family of endothelial markers associated with tumour specific angiogenesis. This study sought to examine the levels of expression for TEMs in human breast cancer. Breast cancer tissues (n = 120) together with normal background tissues (n = 33) were obtained afte...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/b:clin.0000017168.83616.d0
更新日期：2004-01-01 00:00:00
abstract：:Radiation therapy is an effective means of achieving local control in a wide range of primary tumours, with the reduction in the size of the tumour(s) thought to mediate the observed reductions in metastatic spread in clinical trials. However, there is evidence to suggest that the complex changes induced by radiation ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-017-9867-5
更新日期：2018-04-01 00:00:00
abstract：:Hypermethylation of the promoter region of tumor suppressor genes is associated with carcinogenesis in lung cancer (LC). Endobronchial ultrasound with needle aspiration (EBUS-NA) is a semi-invasive method for obtaining cell blocks from lymph nodes, which can be used for epigenetic analyses. To establish the relationsh...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-015-9733-2
更新日期：2015-10-01 00:00:00
abstract：:Accumulating evidences indicate that long non-coding RNAs (lncRNAs) play important roles in several biological processes and dysregulated lncRNAs are involved in different kinds of cancer and are associated with carcinogenesis, metastasis, and prognosis of cancer. The role of a new lncRNA LOC100130476 in gastric cardi...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9794-x
更新日期：2016-06-01 00:00:00
abstract：:We recently developed a method for the isolation and purification of tumour-derived endothelium. In this study the phenotypic and functional properties of human tumour-derived microvascular endothelial cells (TdMEC) were examined. Endothelium obtained from human adrenal gland specimens (HAMEC) was used as a reference ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006738901839
更新日期：1999-01-01 00:00:00
abstract：:Recent studies have indicated that KHT fibrosarcoma or B16 melanoma cell variants capable of forming experimental metastases in the lungs of mice after i.v. injection are created stochastically at high rates (approximately 10(-5)/cell/generation). Expression of this phenotype is unstable and hence expanding population...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00117930
更新日期：1986-07-01 00:00:00
abstract：:To analyze the prognostic value of the extent of peritumoral brain edema in patients operated for single brain metastases (BM), we retrospectively evaluated pre-operative magnetic resonance images in a discovery cohort of 129 patients and a validation cohort of 118 patients, who underwent neurosurgical resection of a ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9542-9
更新日期：2013-04-01 00:00:00
