Abstract:
:MACC1 (metastasis associated in colon cancer 1) is a key driver that induces metastasis in colon cancer. However, the mechanisms by which MACC1 expression is transcriptionally regulated and the factors enriched at the MACC1 promoter remain largely unknown. The binding of proteins to specific DNA sites in the genome is a major determinant of genomic maintenance and the regulation of specific genes. The study herein utilized two methods to study the binding proteins of the MACC1 promoter region in colon cancer. Specifically, we adopted CRISPR-based chromatin affinity purification with mass spectrometry (CRISPR-ChAP-MS) and a biotin-streptavidin pulldown assay coupled with MS to identify the specific proteome bound to the MACC1 promoter in two colon cell lines with different metastatic potential. A total of 24 proteins were identified by CRISPR-ChAP-MS as binding to the MACC1 promoter, among which c-JUN was validated by ChIP-PCR. A total of 739 binding protein candidates were identified by biotin-streptavidin pulldown assays coupled with MS, of which HNF4G and PAX6 were validated and compared for their binding to the same promoter sites in the two cell lines. Our studies suggest distinctive proteomic factors associated with the MACC1 promoter in colon cells with different metastatic potential. The dynamic regulatory factors accumulated at the promoter of MACC1 may provide novel insights into the regulatory mechanisms of MACC1 transcription.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Huang Y,Xiang Y,Xie Z,Cai Y,Yang Q,Huang H,Chen Z,Xiao Z,He Qdoi
10.1007/s10585-020-10045-zsubject
Has Abstractpub_date
2020-08-01 00:00:00pages
477-487issue
4eissn
0262-0898issn
1573-7276pii
10.1007/s10585-020-10045-zjournal_volume
37pub_type
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journal_title:Clinical & experimental metastasis
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journal_title:Clinical & experimental metastasis
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章,评审
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更新日期:2018-08-01 00:00:00
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journal_title:Clinical & experimental metastasis
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doi:10.1007/s10585-012-9559-0
更新日期:2013-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
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doi:10.1007/BF00053899
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