CD133(+) single cell-derived progenies of colorectal cancer cell line SW480 with different invasive and metastatic potential.


:Single cell progenies (SCPs) inherit biological properties from their isogenetic mother cells. If a single cancer cell can give rise to progenies, which can be passaged sustainably in vitro and produce tumor in xenotransplantation, the cell should be cancer initiating cell. CD133 (Prominin-1, Prom1) is the marker of human colorectal cancer (CRC) stem cells and probably a marker of metastatic cancer stem cells (CSCs). Thirty-three SCPs of CRC cell line SW480 were isolated by limited dilution methods, thirty of which are CD133 positive and three negative. All of the CD133(+) SCPs are tumorigenic, and the subcutaneous tumors expanded rapidly, while only 1 of 3 CD133(-) SCPs developed a minimal tumor in nude mice. Orthotopic transplantation experiments showed that CD133(+) SCPs possessed heterogeneity in intestinal wall invasion, lymph node and liver metastases. CD133(+) SCPs varied in cell growth, invasive ability, epithelial-mesenchymal-transition and expression of CSCs markers (CD133, CD44, and CXCR4) associated with metastatic potential. CD133(-) SCPs did not produce secondary transplanted tumor, intestinal invasion and metastasis. The results indicated CD133(+) subpopulation of SW480 SCPs bear heterogeneous invasive and metastatic ability, and CRC-CSCs might be a heterogeous subpopulation.


Clin Exp Metastasis


Li G,Liu C,Yuan J,Xiao X,Tang N,Hao J,Wang H,Bian X,Deng Y,Ding Y




Has Abstract


2010-10-01 00:00:00












  • Histopathologic analysis of bone marrow and bone metastasis in murine neuroblastoma.

    abstract::To elucidate the development of bone metastasis in human neuroblastoma, bone marrow and bone metastases were analysed histologically in a hematogeneous metastasis model of murine neuroblastoma. The bone marrow metastasis occurred initially in the bone marrow sinusoid where tumor cells adhered and extravasated to bone ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Yoshino K,Tanabe M,Ohnuma N,Takahashi H

    更新日期:1996-10-01 00:00:00

  • Augmented autocrine bone morphogenic protein (BMP) 7 signaling increases the metastatic potential of mouse breast cancer cells.

    abstract::As malignant breast cancers progress, they acquire the ability to spread to other regions of the body, including bone and lung, but the molecular mechanism underlying the increase in metastatic potential is not fully understood. Here we studied murine 4T1E/M3 highly bone marrow metastatic breast cancer cells, which we...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Sakai H,Furihata M,Matsuda C,Takahashi M,Miyazaki H,Konakahara T,Imamura T,Okada T

    更新日期:2012-04-01 00:00:00

  • GPR56 plays varying roles in endogenous cancer progression.

    abstract::GPR56, a non-classical adhesion receptor, was previously reported to suppress tumor growth and metastasis in xenograft models using human melanoma cell lines. To understand whether GPR56 plays similar roles in the development of endogenous tumors, we analyzed cancer progression in Gpr56 (-/-) mice using a variety of t...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Xu L,Begum S,Barry M,Crowley D,Yang L,Bronson RT,Hynes RO

    更新日期:2010-04-01 00:00:00

  • Investigation of the role of signal transduction in attachment of ocular melanoma cells to matrix proteins: inhibition of attachment by calmodulin antagonists including tamoxifen.

    abstract::We investigated the role of signal transduction systems in the attachment of human uveal melanoma cells to matrix proteins. Ocular melanoma cells established from primary tumours attached rapidly to all substrates examined. Preferred substrates of attachment were collagens type I, III and IV and fibronectin rather tha...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Mac Neil S,Wagner M,Rennie IG

    更新日期:1994-11-01 00:00:00

  • The histological growth pattern of colorectal cancer liver metastases has prognostic value.

    abstract::Little is known about the biological characteristics that determine the prognosis of colorectal cancer (CRC) liver metastases. In previous work we reported three different histological patterns of the tumour-liver interface of CRC liver metastases, termed the pushing, replacement and desmoplastic growth pattern (GP). ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Van den Eynden GG,Bird NC,Majeed AW,Van Laere S,Dirix LY,Vermeulen PB

    更新日期:2012-08-01 00:00:00

  • Association of SIBA treatment and a Met-depleted diet inhibits in vitro growth and in vivo metastatic spread of experimental tumor cell lines.

    abstract::We have used 5'-deoxy-5'-S isobutyl-thioadenosine (SIBA), an analog of S-adenosylhomocysteine, alone or in association with a methionine-depleted diet in order to obtain an antitumoral effect in two different tumor models: a transplantable rat rhabdomyosarcoma (RMS-J1) induced by i.m. injection of nickel and the well-...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Breillout F,Poupon MF,Blanchard P,Lascaux V,Echinard-Garin P,Robert-Gero M

    更新日期:1988-01-01 00:00:00

  • Effects of gamma irradiation on cultured rat and mouse microvessel endothelial cells: metastatic tumor cell adhesion, subendothelial matrix degradation, and secretion of tumor cell growth factors.

    abstract::The effects of gamma-irradiation on the properties of microvessel endothelial cells were studied in vitro. After incubating confluent endothelial cell monolayers in low serum-containing medium for 24 h, the monolayers were irradiated with 137Cs. Survival of rat lung microvessel endothelial (RLE) and mouse brain microv...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Nicolson GL,Custead SE,Dulski KM,Milas L

    更新日期:1991-09-01 00:00:00

  • Assessment of methylation status of locoregional lymph nodes in lung cancer using EBUS-NA.

    abstract::Hypermethylation of the promoter region of tumor suppressor genes is associated with carcinogenesis in lung cancer (LC). Endobronchial ultrasound with needle aspiration (EBUS-NA) is a semi-invasive method for obtaining cell blocks from lymph nodes, which can be used for epigenetic analyses. To establish the relationsh...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Millares L,Serra M,Andreo F,Sanz-Santos J,Montón C,Grimau C,Gallego M,Setó L,Combalia N,Llatjos M,Escoda R,Castellà E,Monsó E

    更新日期:2015-10-01 00:00:00

  • Dual X-ray absorptiometry detects disease- and treatment-related alterations of bone density in prostate cancer patients.

    abstract::Metastatic bone disease is an important clinical problem which has proven difficult to study because of a lack of noninvasive investigative modalities. Here we show that dual-energy X-ray absorptiometry (DXA) scanning provides clinically useful information about the status of metastatic bone lesions in cancer patients...

    journal_title:Clinical & experimental metastasis

    pub_type: 临床试验,杂志文章


    authors: Smith GL,Doherty AP,Banks LM,Dutton J,Hanham LW,Christmas TJ,Epstein RJ

    更新日期:2000-01-01 00:00:00

  • Editorial: Therapy-induced metastasis.

    abstract::The idea for this Special Issue originated from our recent review in Nature Reviews Clinical Oncology entitled "Does the mobilization of circulating tumour cells during cancer therapy cause metastasis?" Martin et al. (Nat Rev Clin Oncol 14:32-44, 2017). While preparing this review, it became evident that an overwhelmi...

    journal_title:Clinical & experimental metastasis

    pub_type: 社论


    authors: Martin OA,Anderson RL

    更新日期:2018-04-01 00:00:00

  • CA9 gene expression in conventional renal cell carcinoma: a potential marker for prediction of early metastasis after nephrectomy.

    abstract::About 30-40% of patients with renal cell carcinoma (RCC) will develop metastasis after curative nephrectomy. There is a strong need to identify the early metastasis with conventional and molecular risk factors. The present study aimed to test if analysis of the CA9 gene can provide useful information to predict early ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Li G,Feng G,Gentil-Perret A,Genin C,Tostain J

    更新日期:2007-01-01 00:00:00

  • Control pathways of the 67 kDa laminin binding protein: surface expression and activity of a new ligand binding domain.

    abstract::A number of papers have been published on the clinical correlation of the expression of the 67 kDa laminin binding protein (LBP) with the metastatic potential of solid tumors. Both mRNA and protein expression levels have been reported, but both the relationship between them and the molecular nature of the 67 kDa surfa...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Landowski TH,Uthayakumar S,Starkey JR

    更新日期:1995-09-01 00:00:00

  • Analyses of the role of endogenous SPARC in mouse models of prostate and breast cancer.

    abstract::Secreted protein, acidic and rich in cysteine (SPARC, also known as osteonectin or BM-40) is a glycoprotein component of the extracellular matrix that has been reported to be involved with a variety of cellular processes. Although SPARC expression levels are frequently altered in a variety of tumor types, the exact im...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Wong SY,Crowley D,Bronson RT,Hynes RO

    更新日期:2008-01-01 00:00:00

  • MMP-9 secretion and MMP-2 activation distinguish invasive and metastatic sublines of a mouse mammary carcinoma system showing epithelial-mesenchymal transition traits.

    abstract::We have investigated the gelatinase profiles and invasiveness of clonal tumour sublines derived from a spontaneously arising mammary tumour in a Balb/cfC3H mouse. The 67NR. 66c14 and 4T1.2 sublines have low, intermediate and high metastatic potential respectively. In Boyden chamber studies, Matrigel invasion was seen ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Tester AM,Ruangpanit N,Anderson RL,Thompson EW

    更新日期:2000-01-01 00:00:00

  • Circulating microRNAs in head and neck cancer: a scoping review of methods.

    abstract::Circulating microRNAs have been described as head and neck cancer biomarkers in multiple anatomical subsites including the oral cavity, nasopharynx, larynx, salivary glands and the skin. While there is an expanding volume of published literature showing the significance of individual or panels of microRNAs, the clinic...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Dharmawardana N,Ooi EH,Woods C,Hussey D

    更新日期:2019-06-01 00:00:00

  • Ubiquitous Brms1 expression is critical for mammary carcinoma metastasis suppression via promotion of apoptosis.

    abstract::Morbidity and mortality of breast cancer patients are drastically increased when primary tumor cells are able to spread to distant sites and proliferate to become secondary lesions. Effective treatment of metastatic disease has been limited; therefore, an increased molecular understanding to identify biomarkers and th...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Cook LM,Cao X,Dowell AE,Debies MT,Edmonds MD,Beck BH,Kesterson RA,Desmond RA,Frost AR,Hurst DR,Welch DR

    更新日期:2012-04-01 00:00:00

  • A convenient clinically relevant model of human breast cancer bone metastasis.

    abstract::Breast cancer patients with advanced disease exhibit bone metastases, leading to the formation of osteolytic lesions for which the only currently available treatments are palliative. Here, we describe how we refined a mouse model of human breast cancer metastasis into bone, characterized its transcriptome and demonstr...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Garcia T,Jackson A,Bachelier R,Clément-Lacroix P,Baron R,Clézardin P,Pujuguet P

    更新日期:2008-01-01 00:00:00

  • Comparison of metastatic cell lines derived from a murine mammary tumour, and reduction of metastasis by heparin.

    abstract::A murine mammary carcinoma, which had a high potential for metastasis to the lungs, was established in culture, and from the parent line several clonally derived variants were isolated, showing different characteristics including metastatic potential. C1, a high metastatic clone, and C2, a low one, were selected for f...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Lee AE,Rogers LA,Jeffery RE,Longcroft JM

    更新日期:1988-11-01 00:00:00

  • Scatter factor influences the formation of prostate epithelial cell colonies on bone marrow stroma in vitro.

    abstract::Prostate cancer metastases form selectively in the bone marrow. Previously we demonstrated motility was important for the formation of primary prostatic epithelial cell colonies in bone marrow stroma (BMS) co-culture. In this study we looked at the influence of motility factors on the colony formation of epithelial ce...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Lang SH,Clarke NW,George NJ,Testa NG

    更新日期:1999-06-01 00:00:00

  • Growth and metastasis of hypermotile, hyperinvasive cancer cells selected in vitro by rapid locomotion under various conditions.

    abstract::Cancer cells selected from a cultured murine fibrosarcoma by rapid migration through micropore membranes moved considerably faster through such membranes and invaded biological tissues much more efficiently than did the unselected parent cells. The present data show that populations of cells selected by unstimulated m...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Grimstad IA

    更新日期:1988-07-01 00:00:00

  • Tumor cell surface beta 1-6 branched oligosaccharides and lung metastasis.

    abstract::NIH3T3 cells transfected with an activated Ha-ras oncogene were treated with L-PHA, the leukoagglutinin from red kidney beans. Cell lines resistant to L-PHA-mediated cytotoxicity were isolated and found to contain reduced levels of L-PHA-binding oligosaccharides. The levels of N-acetylglucosaminyltransferase V, the en...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Lu Y,Pelling JC,Chaney WG

    更新日期:1994-01-01 00:00:00

  • An improved method to study NK-independent mechanisms of MTLn3 breast cancer lung metastasis.

    abstract::To study the tumor cell autonomous processes of metastasis, an in vivo tumor metastasis model is required that excludes the involvement of the innate immune system. For this purpose we used the established syngeneic MTLn3 cell - Fischer 344 tumor model. MTLn3 cells are efficiently eradicated by NK cells in vivo. Using...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: van Nimwegen MJ,Verkoeijen S,Kuppen PJ,Velthuis JH,van de Water B

    更新日期:2007-01-01 00:00:00

  • Mitogen activated protein kinase pathway is involved in RhoC GTPase induced motility, invasion and angiogenesis in inflammatory breast cancer.

    abstract::Inflammatory breast cancer (IBC) is the most lethal form of locally advanced breast cancer known. IBC carries a guarded prognosis primarily due to rapid onset of disease, typically within six months, and the propensity of tumor emboli to invade the dermal lymphatics and spread systemically. Although the clinical manif...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: van Golen KL,Bao LW,Pan Q,Miller FR,Wu ZF,Merajver SD

    更新日期:2002-01-01 00:00:00

  • Impact of decompressive laminectomy on the functional outcome of patients with metastatic spinal cord compression and neurological impairment.

    abstract::Metastatic spinal cord compression (MSCC) is a frequent phenomenon in advanced tumor diseases with often severe neurological impairments. Affected patients are often treated by decompressive laminectomy. To assess the impact of this procedure on Karnofsky Performance Index (KPI) and Frankel Grade (FG) at discharge, a ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Younsi A,Riemann L,Scherer M,Unterberg A,Zweckberger K

    更新日期:2020-04-01 00:00:00

  • Alterations in Cx43 and OB-cadherin affect breast cancer cell metastatic potential.

    abstract::Emerging evidence suggests that gap junctional intercellular communication (GJIC) and expression of connexins (Cx) contribute to the metastatic potential of breast cancer cells. To more directly address this, an aggressive bone metastasis breast cancer cell line, MDA-MET (MET), was stably transfected with human Cx43 c...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Li Z,Zhou Z,Donahue HJ

    更新日期:2008-01-01 00:00:00

  • Trks are novel oncogenes involved in the induction of neovascularization, tumor progression, and nodal metastasis in oral squamous cell carcinoma.

    abstract::The function of tropomyosin receptor kinase (Trk) family including TrkA, TrkB, and TrkC in cancer remains unknown. The role of Trks in oral squamous cell carcinoma (OSCC) was examined. Knockdown of Trks provided inhibition of growth or invasion and decrease of apoptosis in OSCC cells, which expressed Trks at high leve...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Sasahira T,Ueda N,Yamamoto K,Bhawal UK,Kurihara M,Kirita T,Kuniyasu H

    更新日期:2013-02-01 00:00:00

  • Human-specific RNA analysis shows uncoupled epithelial-mesenchymal plasticity in circulating and disseminated tumour cells from human breast cancer xenografts.

    abstract::Blood samples, bone marrow, tumours and metastases where possible were collected from SCID mice bearing orthotopic xenografts of the triple-negative MDA-MB-468 cell line or a transplantable ER-positive patient derived xenograft (ED-03), and assessed using human-specific, tandem-nested RT-qPCR for markers relating to d...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Tachtsidis A,Le AV,Blick T,Gunasinghe D,De Sousa E,Waltham M,Dobrovic A,Thompson EW

    更新日期:2019-08-01 00:00:00

  • Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis.

    abstract::Metastasis is key to cancer mortality. Understanding its biology is vital for developing strategies to prevent and treat metastasis. Phenotypic assays to either study metastasis or evaluate anti-metastatic drugs are widely used in preclinical research. This technical note discusses the adherence of reporting essential...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Boiy R,Steenbrugge J,Van Deun J,Hendrix A,Meyer E,De Wever O

    更新日期:2018-12-01 00:00:00

  • Metastasis predictive signature profiles pre-exist in normal tissues.

    abstract::Previous studies from our laboratory have demonstrated that metastatic propensity is significantly influenced by the genetic background upon which tumors arise. We have also established that human gene expression profiles predictive of metastasis are not only present in mouse tumors with both high and low metastatic c...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Yang H,Crawford N,Lukes L,Finney R,Lancaster M,Hunter KW

    更新日期:2005-01-01 00:00:00

  • Metastatic potential of human CX-1 colon adenocarcinoma cells is dependent on the expression of sialosyl Le(a) antigen.

    abstract::Several lines of evidence indicate that sialosyl Le(a), tumor-associated carbohydrate antigen present on human colon carcinoma cells, is involved in formation of metastases. To study the role of this carbohydrate structure in development of metastases, we have used the clone of human colon carcinoma CX-1 cells transfe...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Opolski A,Laskowska A,Madej J,Wietrzyk J,Kłopocki A,Radzikowski C,Ugorski M

    更新日期:1998-11-01 00:00:00