Abstract：
:Recently the concept that gap junctions play a role in cancer cell metastasis has emerged. However, the mechanism by which this might occur is unknown. To examine this issue a metastatic breast cancer cell line, MDA-MB-435, was stably transfected with human Cx43 cDNA. Four clones of 435 transfectants (435/Cx43(+) c1, c6, c8, c14) and two clones of plasmid control (435/hy) were isolated and examined in this study. We found that expressing Cx43 in MDA-MB-435 cells decreased their expression of Cx32 but did not affect gap junctional intercellular communication, migration or invasion through Matrigel((R)). However, forced expression of Cx43 decreased the growth of MDA-MB-435 cells, decreased expression of N-cadherin, which is frequently associated with an aggressive phenotype, and increased MDA-MB-435 sensitivity to apoptosis. More importantly, there were fewer lung metastases in mice injected with 435/Cx43(+) cells relative to mice injected with 435/hy. These results suggest that expressing Cx43 in breast cancer cells decreases their metastatic potential through a mechanism independent of gap junctional communication but, rather, related to N-cadherin expression and apoptosis.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Li Z,Zhou Z,Welch DR,Donahue HJdoi
10.1007/s10585-008-9208-9subject
Has Abstractpub_date
2008-01-01 00:00:00pages
893-901issue
8eissn
0262-0898issn
1573-7276journal_volume
25pub_type
杂志文章abstract：BACKGROUND:One of the major clinical problems in breast cancer detection is the relatively high incidence of occult lymph node metastases undetectable by standard procedures. Since the ascertainment of breast cancer stage determines the following treatment, such a "hypo-diagnosis" leads to inadequate therapy, and hence...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9135-1
更新日期：2008-01-01 00:00:00
abstract：:The effect of thioglycollate-elicited macrophages (TG-M phi) on natural killer (NK)-cell activity and metastases formation in mice was investigated. Intravenously (i.v.) inoculated TG-M phi inhibited spleen NK activity of normal mice and abrogated polyinosinic: polycytidylic (poly I:C) induced augmentation of NK cell ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01758960
更新日期：1985-04-01 00:00:00
abstract：:Breast cancer has a predilection for metastasis to the bone marrow. The preprotachykinin-I (PPT-I) gene has a central role in the early migration of breast cancer cells into the bone marrow, making this organ a latent repository of the cancer cells. This study investigated whether the invasive and metastatic potential...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-006-9001-6
更新日期：2005-01-01 00:00:00
abstract：:The effects of the cyclooxygenase (COX)-2 inhibitor nimesulide on bombesin-enhanced peritoneal metastasis of azoxymethane (AOM)-induced intestinal adenocarcinomas were investigated in male Wistar rats. From the beginning of the study, the rats were given 10 weekly s.c. injections of AOM (7.4 mg/kg body weight) and s.c...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1025883129932
更新日期：2003-01-01 00:00:00
abstract：:Evidence suggests that multiple tumors, including pancreatic adenocarcinoma, display heterogeneity in parameters that are critical for tumor formation, progression and metastasis. Understanding heterogeneity in solid tumors is increasingly providing a plethora of new diagnostic and therapeutic approaches. In this stud...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-009-9260-0
更新日期：2009-01-01 00:00:00
abstract：:Secreted protein, acidic and rich in cysteine (SPARC, also known as osteonectin or BM-40) is a glycoprotein component of the extracellular matrix that has been reported to be involved with a variety of cellular processes. Although SPARC expression levels are frequently altered in a variety of tumor types, the exact im...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9126-2
更新日期：2008-01-01 00:00:00
abstract：:This study was carried out to clarify the presentation, treatment options, and prognosis of renal cell carcinoma (RCC) metastasis to the bladder in which we do not yet have a comprehensive understanding. A systematic Medline, Web of Science, Embase, Google, and Ichushi Web search was performed to identify articles des...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-015-9698-1
更新日期：2015-02-01 00:00:00
abstract：:Primary brain tumors lack the metastatic behavior that is in part believed to be promoted by the extracellular matrix (ECM) components of the basement membrane. This study was intended to examine the influence of the ECM components present in the basement membrane that may act as natural barriers to tumor cell invasio...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00123395
更新日期：1996-09-01 00:00:00
abstract：:In this study the relationship between brain structure and brain metastases (BM) occurrence was analyzed. A model for predicting the time of BM onset in patients with non-small cell lung cancer (NSCLC) was proposed. Twenty patients were used to develop the model, whereas the remaining 69 were used for independent vali...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9833-7
更新日期：2017-02-01 00:00:00
abstract：:It is generally accepted that recent advances in anticancer agents have contributed significantly to the improvement of both the disease-free survival and quality of life in cancer patients. However, in many instances, a favorable initial response to treatment changes afterwards, thereby leading to cancer relapse and ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-018-9903-0
更新日期：2018-04-01 00:00:00
abstract：:Sentinel lymph node (SLN) identification with preoperative image guidance may improve pathological staging of prostate cancer by identifying nodes outside the standard template of dissection. Four anesthetized male dogs received an intra-prostatic injection of Ga-68-labeled tilmanocept. Every 20 min the pelvic lymph n...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9498-9
更新日期：2012-10-01 00:00:00
abstract：:The effects of gamma-irradiation on the properties of microvessel endothelial cells were studied in vitro. After incubating confluent endothelial cell monolayers in low serum-containing medium for 24 h, the monolayers were irradiated with 137Cs. Survival of rat lung microvessel endothelial (RLE) and mouse brain microv...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01785531
更新日期：1991-09-01 00:00:00
abstract：:Two widely used B16 melanoma cell lines of low and high lung colonizing potential (B16-F1 and B16-F10) were compared in their ability to induce platelet aggregation. The results of these experiments showed a reproducible difference in platelet aggregating activity of these two cell lines which directly correlated with...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00058058
更新日期：1987-04-01 00:00:00
abstract：:Tumor cells exposed to a growth stress such as low pH, glucose starvation and hypoxia have been shown to exhibit a transient increase in experimental metastatic potential, particularly when allowed to recover under normal growth conditions for a period of 24-48 h. In this study we examined whether this increase in met...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1018470709523
更新日期：1997-09-01 00:00:00
abstract：:This short review offers an update on the first and second Multicenter Selective Lymphadenectomy Trials (MSLT-I and MSLT-II) for patients with melanoma, and briefly traces the development of intraoperative lymphatic mapping and sentinel node biopsy. ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,多中心研究,评审
doi：10.1007/s10585-012-9503-3
更新日期：2012-10-01 00:00:00
abstract：:The urokinase-type plasminogen activator (UPA) and its inhibitor PAI-1 are thought to play an important part in gastric cancer (GC) invasion and metastasis. Little is known about the behavior and prognostic impact of the receptor for UPA (UPAR). The aims of the present study were: (1) to measure UPAR, UPA and PAI-1 le...
journal_title：Clinical & experimental metastasis
pub_type： 临床试验,杂志文章
doi：10.1023/a:1018454305889
更新日期：1997-07-01 00:00:00
abstract：:Extrathyroidal extension (ETE) of papillary thyroid carcinoma (PTC) is common and clinical presentation can vary from minimal to extensive locoregional involvement. Although PTC is generally considered the most benign among all thyroid carcinomas, it may present with local invasion with poor prognosis. Our retrospecti...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9800-3
更新日期：2016-08-01 00:00:00
abstract：:Human fibrosarcoma HT1080 cell surface phenotype analysis revealed the expression of "cluster of differentiation 15" (CD15) antigen and to a lesser extent, of "very late antigen-4" (VLA-4). Expression of "endothelial-leukocyte adhesion molecule-1" (ELAM-1) was negligible on resting human umbilical vascular endothelial...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1018487213157
更新日期：1997-11-01 00:00:00
abstract：:As a reliable model for osteosarcoma is lacking, three human cell lines (SaOS-2, U2OS and 143B) were evaluated in cell-based assays for proliferation, adhesion, migration, invasion, anchorage-independent growth, angiogenesis, mineralised nodule formation, plasmid transfection and oligonucleotide transfection. Tumor ta...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-006-9046-6
更新日期：2006-01-01 00:00:00
abstract：:Anti-resorptive bisphosphonates (BPs) have been clinically used to prevent cancer-bone metastasis and cancer-induced bone pathologies despite the fact that the phenotypic response of the cancer-bone interactions to BP exposure is "uncharted territory". This study offers unique insights into the interplay between cance...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9798-6
更新日期：2016-08-01 00:00:00
abstract：:We investigated the role of signal transduction systems in the attachment of human uveal melanoma cells to matrix proteins. Ocular melanoma cells established from primary tumours attached rapidly to all substrates examined. Preferred substrates of attachment were collagens type I, III and IV and fibronectin rather tha...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01755881
更新日期：1994-11-01 00:00:00
abstract：:Primary tumor growth and metastasis depend on angiogenesis, which is determined by the balance between proangiogenic and antiangiogenic molecules. Interferon (IFN)-alpha and -beta inhibit angiogenesis through downregulation of interleukin-8, matrix metalloproteinase-9, and basic fibroblast growth factor. To provide ev...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1020923326441
更新日期：2002-01-01 00:00:00
abstract：:This study examined the relationship between the expression of E-cadherin or beta-catenin in murine adenocarcinomas and their hematogenous metastatic propensity, assessed by both spontaneous and artificial lung metastasis. Seven different carcinomas, syngeneic to C3Hf/Kam mice were used: 4 mammary carcinomas (MCa-4, M...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006670918848
更新日期：1999-03-01 00:00:00
abstract：:High grade serous ovarian tumors often metastasize transperitoneally, a process that begins when small tumor nodules de-adhere and are released into the fluid of the abdominal cavity where they float freely to reach new sites on the peritoneal wall. Podocalyxin, a small anti-adhesive sialomucin, has been shown to cont...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-011-9446-0
更新日期：2012-03-01 00:00:00
abstract：:Clear cell renal cell carcinoma (ccRCC) is one of few cancers with rising incidence in North America. The prognosis of ccRCC is variable and difficult to predict. Stratification of patients according to disease aggressiveness can significantly improve patient management. We investigated the expression of the S100A11 p...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-015-9758-6
更新日期：2016-01-01 00:00:00
abstract：:RGD-containing substrates were used to study static and hydrodynamic adhesion of murine RAW117 large-cell lymphoma sublines with differential liver-metastatic potentials. Highly liver-metastatic RAW117-H10 cells had higher rates of static adhesion to vitronectin, fibronectin and (GRGDS)4 than poorly metastatic RAW117-...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1018451616309
更新日期：1997-01-01 00:00:00
abstract：:We have compared the pattern of surface antigen expression, as detected by monoclonal antibodies (mAbs), in plasma membranes vs shed membrane vesicles of two human breast carcinoma cell lines, MCF-7 and 8701-BC. Antigen expression was detected on cells by immunofluorescence (IF) analysis, whilst, due to their small di...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00133483
更新日期：1995-07-01 00:00:00
abstract：:Lung cancer often metastasizes to bone in patients with advanced disease. Identification of the factors involved in the interactions between lung cancer cells and bone will improve the prevention and treatment of bone metastases. We identified changes in metastasis-related gene expression of human HARA lung squamous c...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9059-9
更新日期：2007-01-01 00:00:00
abstract：:Brain metastases present a significant public health issue, affecting more than 100,000 patients per year in the U.S. and result in significant morbidity. Brain metastases can occur in a variety of clinical situations ranging from multiple brain metastases with uncontrolled systemic disease to a solitary metastasis in...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-017-9860-z
更新日期：2017-10-01 00:00:00
abstract：:Signal peptide-CUB-EGF-like domain-containing protein 3 (SCUBE3) is strongly expressed in extremely invasive lung carcinoma. We showed in our previous study that SCUBE3 triggers the transforming growth factor-β pathway and subsequently promotes tumor angiogenesis and the epithelial-mesenchymal transition (EMT). Howeve...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9575-8
更新日期：2013-08-01 00:00:00
