Abstract：
:We investigated the role of signal transduction systems in the attachment of human uveal melanoma cells to matrix proteins. Ocular melanoma cells established from primary tumours attached rapidly to all substrates examined. Preferred substrates of attachment were collagens type I, III and IV and fibronectin rather than laminin, gelatin, arginine-glycine-aspartine, vitronectin, poly-L-lysine or plastic. All cells showed rapid attachment to the preferred substrates (80% within 10 min). Manipulation of intracellular cyclic AMP or protein kinase C activity had relatively little effect on cell attachment. In contrast, attachment was significantly reduced by manipulating either intracellular calcium or calmodulin. After 15 min at 37 degrees C, the calcium ionophore ionomycin (5 microM) reduced attachment to 25%, and TMB8 (50 microM), which can reduce intracellular calcium, reduced attachment to 60%. The experimental calmodulin antagonist J8 (25 microM), a substituted naphthalene sulphonamide, reduced attachment to 40%. Similarly tamoxifen (25 microM), which has calmodulin antagonist activity in vitro, reduced attachment to 55%. Both J8 and tamoxifen inhibited cell attachment to a wide range of matrix proteins, suggesting that this effect on attachment is not dependent on the presence of specific adhesion receptors. Reduction of ocular melanoma tumour cell/matrix interactions through manipulation of intracellular calcium or calmodulin may therefore merit further investigation as a possible approach to reducing metastatic spread.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Mac Neil S,Wagner M,Rennie IGdoi
10.1007/BF01755881subject
Has Abstractpub_date
1994-11-01 00:00:00pages
375-84issue
6eissn
0262-0898issn
1573-7276journal_volume
12pub_type
杂志文章abstract：:Mouse mammary carcinoma cells were exposed in vitro to increasing concentrations of doxorubicin hydrochloride [adriamycin (ADR)] or 5-fluorouracil (5-FU). Uptake of [75Se]selenomethionine (75SeM) in a methionine-deficient medium measured the resulting inhibition of protein synthesis by the tumour cells. This was compa...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01753663
更新日期：1989-07-01 00:00:00
abstract：:Smad interacting protein 1 (SIP1) is an epithelial-mesenchymal transition (EMT)-inducible gene that plays a key role in tumor progression in various cancers. This study seeks to clarify the clinical and biological significance of SIP1 expression, especially in intestinal type gastric cancer. We analyzed the mRNA level...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9547-4
更新日期：2013-04-01 00:00:00
abstract：:Cancer cells selected from a cultured murine fibrosarcoma by rapid migration through micropore membranes moved considerably faster through such membranes and invaded biological tissues much more efficiently than did the unselected parent cells. The present data show that populations of cells selected by unstimulated m...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01753573
更新日期：1988-07-01 00:00:00
abstract：:Emerging evidence suggests that gap junctional intercellular communication (GJIC) and expression of connexins (Cx) contribute to the metastatic potential of breast cancer cells. To more directly address this, an aggressive bone metastasis breast cancer cell line, MDA-MET (MET), was stably transfected with human Cx43 c...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9140-4
更新日期：2008-01-01 00:00:00
abstract：:Metastatic spinal cord compression (MSCC) is a frequent phenomenon in advanced tumor diseases with often severe neurological impairments. Affected patients are often treated by decompressive laminectomy. To assess the impact of this procedure on Karnofsky Performance Index (KPI) and Frankel Grade (FG) at discharge, a ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-019-10016-z
更新日期：2020-04-01 00:00:00
abstract：:Breast cancer metastasizes to bone with high frequency and incidence. However, studies of breast cancer metastasis to bone have been limited by two factors. First, the number of models that colonize bone are limited. Second, detection of bone metastases is too insensitive or too laborious for routine, large-scale stud...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1024062911144
更新日期：2003-01-01 00:00:00
abstract：:Integrins expressed on endothelial cells modulate cell migration and survival during angiogenesis. Integrins expressed on carcinoma cells potentiate metastasis by facilitating invasion and movement across blood vessels. We describe the activities of two synthetic low-molecular-weight peptidomimetics of the ligand amin...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/b:clin.0000024764.93092.5f
更新日期：2004-01-01 00:00:00
abstract：:Tumor cell metastasis can be suppressed by the attenuation of proteolytic and angiogenic events that are mediated by tumor and endothelial cells. Combinations of specific inhibitors directed to separate stages of the metastatic cascade may improve the potential for adjuvant therapies. Amiloride is an effective plasmin...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/b:clin.0000046140.19131.19
更新日期：2004-01-01 00:00:00
abstract：:Melanoma is an aggressive cutaneous malignancy with rapidly rising incidence. Diagnosis of controversial melanocytic lesions, correct prognostication of patients, selection of appropriate adjuvant and systemic therapies, and prediction of response to a given therapy remain very real challenges. Despite these challenge...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-018-9892-z
更新日期：2018-08-01 00:00:00
abstract：:Human CD44 standard isoform (hCD44s) cDNA regulated by a high-expressing promoter was transfected into Balb/c 3T3 cells and the tumorigenic and metastatic capacities of the transfectants were investigated in nude mice at the subcutaneous site. One of three transfectants was tumorigenic. hCD44s expression was lost in t...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006568103588
更新日期：1998-01-01 00:00:00
abstract：:Solid tumors consist of genetically and phenotypically diverse subpopulations of cancer cells with unique capacities for growth, differentiation, and invasion. While the molecular and microenvironmental bases for heterogeneity are increasingly appreciated, the outcomes of such intratumor heterogeneity, particularly in...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9565-x
更新日期：2013-06-01 00:00:00
abstract：:MACC1 (metastasis associated in colon cancer 1) is a key driver that induces metastasis in colon cancer. However, the mechanisms by which MACC1 expression is transcriptionally regulated and the factors enriched at the MACC1 promoter remain largely unknown. The binding of proteins to specific DNA sites in the genome is...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-020-10045-z
更新日期：2020-08-01 00:00:00
abstract：:About 30-40% of patients with renal cell carcinoma (RCC) will develop metastasis after curative nephrectomy. There is a strong need to identify the early metastasis with conventional and molecular risk factors. The present study aimed to test if analysis of the CA9 gene can provide useful information to predict early ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9064-z
更新日期：2007-01-01 00:00:00
abstract：:The present study assessed the effects of dietary fat on spontaneous metastasis of Lewis lung carcinoma in mice. Three-week old male C57BL/6 mice were fed the AIN-93G diet or a 45% fat diet (% kcal.) for 7 weeks before they were subcutaneously injected with 2.5 × 10⁵ viable carcinoma cells. The primary tumor was resec...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-010-9347-7
更新日期：2010-12-01 00:00:00
abstract：:The effect of thioglycollate-elicited macrophages (TG-M phi) on natural killer (NK)-cell activity and metastases formation in mice was investigated. Intravenously (i.v.) inoculated TG-M phi inhibited spleen NK activity of normal mice and abrogated polyinosinic: polycytidylic (poly I:C) induced augmentation of NK cell ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01758960
更新日期：1985-04-01 00:00:00
abstract：:Breast cancer (BC) is the most common cancer affecting women in the United States and metastatic breast cancer is the leading cause of death. The role estradiol plays in ER-positive BC is well-documented, but the way it contributes to ER-negative BC remains unclear. In the present study, we utilized an experimental mo...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9559-0
更新日期：2013-08-01 00:00:00
abstract：:The amount and type of sialylation of tumor cell membranes depends on the activity of a number of different sialyltransferase enzymes. For the detection of specific activities in human colorectal carcinoma tissue several glycoprotein and glycolipid acceptors were used: desialylated fetuin, alpha 1-acid glycoprotein, b...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01753893
更新日期：1994-05-01 00:00:00
abstract：:The spinal column is the most frequent site of bone metastasis in patients with breast cancer. It is important to understand how the pattern of vertebral lesions may be affected by the introduction of modern cancer therapies. The purpose of this study was to characterize changes in the radiological appearance of spina...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-008-9219-6
更新日期：2009-01-01 00:00:00
abstract：:Metastatic bone disease is an important clinical problem which has proven difficult to study because of a lack of noninvasive investigative modalities. Here we show that dual-energy X-ray absorptiometry (DXA) scanning provides clinically useful information about the status of metastatic bone lesions in cancer patients...
journal_title：Clinical & experimental metastasis
pub_type： 临床试验,杂志文章
doi：10.1023/a:1010991213842
更新日期：2000-01-01 00:00:00
abstract：:Brain metastases present a significant public health issue, affecting more than 100,000 patients per year in the U.S. and result in significant morbidity. Brain metastases can occur in a variety of clinical situations ranging from multiple brain metastases with uncontrolled systemic disease to a solitary metastasis in...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-017-9860-z
更新日期：2017-10-01 00:00:00
abstract：:Considering recent findings that cyclooxygensase-2 (COX-2) is involved in the progression of colorectal carcinoma (CRC), the role of COX-2 in promoting invasion and angiogenesis was investigated by evaluating the relationship of COX-2 expression to various clinicopathological variables, including plasminogen activatin...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1020392309715
更新日期：2002-01-01 00:00:00
abstract：:To aid in therapy selection for patients with spinal bone metastases (SBM), predictive models have been developed. These models consider SBM from breast cancer a positive predictive factor, but do not take phenotypes based on estrogen (ER), progesterone (PR) and human epidermal growth factor 2 (HER2) receptors into ac...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,多中心研究
doi：10.1007/s10585-014-9685-y
更新日期：2015-01-01 00:00:00
abstract：:Intravenous infusions of perfluorochemical emulsions, combined with administration of inspired oxygen or carbogen have been found to improve tumor oxygenation and increase the response of solid tumors in animals to radiotherapy. Fluosol-DA 20 per cent, the only perfluorochemical emulsion currently approved for testing...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00053472
更新日期：1986-01-01 00:00:00
abstract：:For more effective oncological management of disseminated colorectal cancer, therapies must be devised that target the different individual stages of metastasis development. Recent work showed that integrin subunits alpha2, alpha6 and beta4 are involved in the colorectal cancer cell extravasation process. By means of ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-009-9276-5
更新日期：2009-01-01 00:00:00
abstract：:We previously isolated thymosin beta15 from highly metastatic Dunning rat prostatic carcinoma cells. Immunohistochemical study of human prostate cancer specimens revealed a general correlation between Gleason grade and thymosin beta15 expression, with high-grade (more malignant) tumors showing increased staining compa...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006540824969
更新日期：1998-04-01 00:00:00
abstract：:We applied reverse phase protein microarrays technology to map signal pathway interactions in a discovery set of 34 soft tissue sarcoma (STS) bone metastases compared to healthy bone. Proteins associated with matrix remodeling (MMP), adhesion (FAK Y576/577, Syndecan-1), and growth/survival (IGF1R Y1135/1136, PI3K, EGF...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9605-6
更新日期：2014-01-01 00:00:00
abstract：:A number of papers have been published on the clinical correlation of the expression of the 67 kDa laminin binding protein (LBP) with the metastatic potential of solid tumors. Both mRNA and protein expression levels have been reported, but both the relationship between them and the molecular nature of the 67 kDa surfa...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00121912
更新日期：1995-09-01 00:00:00
abstract：:Although in the past little attention has been paid to the influence of osteosarcoma cells in osteoclast function, recent studies suggest a close relationship between osteosarcoma aggressiveness and osteoclastic activity. The present study addresses the paracrine effects of MG63 cells, a human osteosarcoma-derived cel...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-011-9387-7
更新日期：2011-08-01 00:00:00
abstract：BACKGROUND:One of the major clinical problems in breast cancer detection is the relatively high incidence of occult lymph node metastases undetectable by standard procedures. Since the ascertainment of breast cancer stage determines the following treatment, such a "hypo-diagnosis" leads to inadequate therapy, and hence...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9135-1
更新日期：2008-01-01 00:00:00
abstract：:A new model for the study of spontaneous bone metastasis has been developed which allows for the quantification of metastatic tumor burden and cancer cell growth rate, and which describes the progressive changes in bone morphology. Walker 256 (W256) cells or vehicle were injected into the left upper thigh muscle of ma...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00133469
更新日期：1992-11-01 00:00:00
