Abstract：
:Human CD44 standard isoform (hCD44s) cDNA regulated by a high-expressing promoter was transfected into Balb/c 3T3 cells and the tumorigenic and metastatic capacities of the transfectants were investigated in nude mice at the subcutaneous site. One of three transfectants was tumorigenic. hCD44s expression was lost in the cells of large primary tumors using this tumorigenic clone. These tumors were extremely aggressive giving overt metastases and micrometastases to several sites including mesentery, stomach, liver, diaphragm, pancreas and lung. Micrometastatic cells re-expressed hCD44s, consistent with its importance for early steps in the metastatic cascade. hCD44s was not expressed in overt metastases; most probably the expression was lost during the outgrowth of micrometastases into overt metastatic tumors. Thus hCD44s expression in murine 3T3 cells does induce tumorigenicity in select cases, is not compatible with aggressive outgrowth of primary or secondary tumors, and is advantageous for early steps in metastatic spread. These results suggest that CD44s is an example of a novel type of 'metastasis' molecule that is disadvantageous for tumor growth and is only transiently advantageous during metastatic spreading of tumor cells to distant organs.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Kogerman P,Sy MS,Culp LAdoi
10.1023/a:1006568103588subject
Has Abstractpub_date
1998-01-01 00:00:00pages
83-93issue
1eissn
0262-0898issn
1573-7276journal_volume
16pub_type
杂志文章abstract：:Solid tumors consist of genetically and phenotypically diverse subpopulations of cancer cells with unique capacities for growth, differentiation, and invasion. While the molecular and microenvironmental bases for heterogeneity are increasingly appreciated, the outcomes of such intratumor heterogeneity, particularly in...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9565-x
更新日期：2013-06-01 00:00:00
abstract：:Spatial and temporal oxygen heterogeneity exists in most solid tumour microenvironments due to an inadequate vascular network supplying a dense population of tumour cells. An imbalance between oxygen supply and demand leads to hypoxia within a significant proportion of a tumour, which has been correlated to the likeli...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9139-x
更新日期：2008-01-01 00:00:00
abstract：:Several lines of evidence indicate that sialosyl Le(a), tumor-associated carbohydrate antigen present on human colon carcinoma cells, is involved in formation of metastases. To study the role of this carbohydrate structure in development of metastases, we have used the clone of human colon carcinoma CX-1 cells transfe...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006502009682
更新日期：1998-11-01 00:00:00
abstract：:Sentinel lymph node (SLN) mapping is common, however question remains as to what the ideal imaging agent is and how such an agent might provide reliable and stable localization of SLNs. (99m)Tc-labeled nanocolloid human serum albumin (Nanocoll) is the most commonly used radio-labeled colloid in Europe and remains the ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s10585-012-9497-x
更新日期：2012-10-01 00:00:00
abstract：:Two widely used B16 melanoma cell lines of low and high lung colonizing potential (B16-F1 and B16-F10) were compared in their ability to induce platelet aggregation. The results of these experiments showed a reproducible difference in platelet aggregating activity of these two cell lines which directly correlated with...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00058058
更新日期：1987-04-01 00:00:00
abstract：:The effect of thioglycollate-elicited macrophages (TG-M phi) on natural killer (NK)-cell activity and metastases formation in mice was investigated. Intravenously (i.v.) inoculated TG-M phi inhibited spleen NK activity of normal mice and abrogated polyinosinic: polycytidylic (poly I:C) induced augmentation of NK cell ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01758960
更新日期：1985-04-01 00:00:00
abstract：:To evaluate the relationship between the c-kit proto-oncogene product and malignant transformation of human breast tissue, we examined the immunohistochemical expression of the c-kit proto-oncogene product in both malignant and non-malignant breast tissues. The immunohistochemical expression of the c-kit proto-oncogen...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1027323210736
更新日期：2003-01-01 00:00:00
abstract：:Bone sialoprotein (BSP) and osteopontin (OPN) are important factors in the metastasis of breast cancer, which were examined as targets for antineoplastic therapy by siRNA. In addition, the effect of gene silencing on their transcription factor Runx2 and their interaction partners integrin β(3) and matrix metalloprotei...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9462-8
更新日期：2012-06-01 00:00:00
abstract：:Protein kinase C (PKC) has been shown to be a signal transducer during tumorigenesis, tumor cell invasion, and metastasis. Recent studies have reported that the PKC inhibitor, 7-hydroxystaurosporine, inhibits tumor cell invasion. However, the molecular mechanisms of this inhibition of invasion and metastasis are not w...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9080-z
更新日期：2007-01-01 00:00:00
abstract：:Metastatic bone disease is an important clinical problem which has proven difficult to study because of a lack of noninvasive investigative modalities. Here we show that dual-energy X-ray absorptiometry (DXA) scanning provides clinically useful information about the status of metastatic bone lesions in cancer patients...
journal_title：Clinical & experimental metastasis
pub_type： 临床试验,杂志文章
doi：10.1023/a:1010991213842
更新日期：2000-01-01 00:00:00
abstract：:Bone is the third most common site of cancer metastasis. Over 30 to 40% of lung cancers can develop skeletal metastasis and no effective curative therapy exists in clinic cases. Previously we screened the different expression of proteins between SBC-5 cells and SBC-3 cells by proteomic study methods (MALDI-TOF/TOF-MS)...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-011-9408-6
更新日期：2011-12-01 00:00:00
abstract：:Mouse mammary carcinoma cells were exposed in vitro to increasing concentrations of doxorubicin hydrochloride [adriamycin (ADR)] or 5-fluorouracil (5-FU). Uptake of [75Se]selenomethionine (75SeM) in a methionine-deficient medium measured the resulting inhibition of protein synthesis by the tumour cells. This was compa...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01753663
更新日期：1989-07-01 00:00:00
abstract：:It is recognised that stromal cells determine cancer progression. We have previously shown that active TGFbeta produced by rat colon carcinoma cells modulated NO production in rat endothelial cells. To elucidate the role of TGFbeta and NO in the mechanisms of interaction of colon carcinoma cells with stromal cells and...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-005-0431-3
更新日期：2005-01-01 00:00:00
abstract：:Lung cancer often metastasizes to bone in patients with advanced disease. Identification of the factors involved in the interactions between lung cancer cells and bone will improve the prevention and treatment of bone metastases. We identified changes in metastasis-related gene expression of human HARA lung squamous c...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9059-9
更新日期：2007-01-01 00:00:00
abstract：:Metastasis remains the major cause of therapeutic failure, poor prognosis and high mortality in breast and prostate cancer patients. Aberrant microenvironments including hypoxia and acidic pH are common features of most solid tumors that have been long associated with enhanced metastasis and poor patient outcomes. Nov...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9590-9
更新日期：2013-10-01 00:00:00
abstract：:The function of tropomyosin receptor kinase (Trk) family including TrkA, TrkB, and TrkC in cancer remains unknown. The role of Trks in oral squamous cell carcinoma (OSCC) was examined. Knockdown of Trks provided inhibition of growth or invasion and decrease of apoptosis in OSCC cells, which expressed Trks at high leve...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9525-x
更新日期：2013-02-01 00:00:00
abstract：:Matrilysin is a member of the matrix metalloproteinase gene family which is believed to play an important role in tumor progression. Expression of matrilysin mRNA was examined by reverse transcription-polymerase chain reaction combined with Southern blot analysis in 46 human primary gastric cancers. Overexpression of ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006509312674
更新日期：1998-05-01 00:00:00
abstract：:Circulating microRNAs have been described as head and neck cancer biomarkers in multiple anatomical subsites including the oral cavity, nasopharynx, larynx, salivary glands and the skin. While there is an expanding volume of published literature showing the significance of individual or panels of microRNAs, the clinic...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-019-09961-6
更新日期：2019-06-01 00:00:00
abstract：:We have used 5'-deoxy-5'-S isobutyl-thioadenosine (SIBA), an analog of S-adenosylhomocysteine, alone or in association with a methionine-depleted diet in order to obtain an antitumoral effect in two different tumor models: a transplantable rat rhabdomyosarcoma (RMS-J1) induced by i.m. injection of nickel and the well-...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01580402
更新日期：1988-01-01 00:00:00
abstract：:High grade serous ovarian tumors often metastasize transperitoneally, a process that begins when small tumor nodules de-adhere and are released into the fluid of the abdominal cavity where they float freely to reach new sites on the peritoneal wall. Podocalyxin, a small anti-adhesive sialomucin, has been shown to cont...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-011-9446-0
更新日期：2012-03-01 00:00:00
abstract：:This study compared phenotype and behaviour of seven head and neck squamous cell carcinoma (HNSCC) cell lines and normal epithelial cells. Indications were found that in HNSCC cells: 1) loss of the cell-cell adhesion molecule E-cadherin was correlated with loss of epithelioid cell morphology but not with loss of cell-...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00115110
更新日期：1996-11-01 00:00:00
abstract：:Radiotherapy induces untargeted effects on normal tissues such as bone marrow. So alteration of microenvironment by ionizing irradiation is supposed to influence dynamic host-cancer ecosystem affecting cancer behavior including metastasis. Herein, the incidence of lung metastasis after high-dose irradiation has been i...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-011-9411-y
更新日期：2011-12-01 00:00:00
abstract：:To provide an investigative tool for the study of osteosarcoma (OSA) biology we have developed a syngeneic (balb/c) murine model of OSA, using cell lines derived from a spontaneously occurring murine OSA (Schmidt et al. Differentiation 1988; 39: 151-60). This model is characterized by orthotopic primary tumor growth, ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006767007547
更新日期：2000-01-01 00:00:00
abstract：:CNTO 95 is a fully human monoclonal antibody that recognizes alphav integrins. Previous studies have shown that CNTO 95 exhibits both anti-tumor and anti-angiogenic activities (Trikha M et al., Int J Cancer 110:326-335, 2004). In this study we investigated the biological activities of CNTO 95 on breast tumor cells bot...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9132-4
更新日期：2008-01-01 00:00:00
abstract：:We previously isolated thymosin beta15 from highly metastatic Dunning rat prostatic carcinoma cells. Immunohistochemical study of human prostate cancer specimens revealed a general correlation between Gleason grade and thymosin beta15 expression, with high-grade (more malignant) tumors showing increased staining compa...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006540824969
更新日期：1998-04-01 00:00:00
abstract：:The effects of treatment of human lung carcinoma cell line A 549 with recombinant DNA-derived human leukocyte interferons A (rIFN-alpha A) or D (rIFN-alpha D), and human lymphoblastoid interferon (Wellferon) on in vitro cell invasion were investigated in a quantitative invasion assay using human amnion. The A 549 cell...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00117932
更新日期：1986-07-01 00:00:00
abstract：:Pancreatic cancer demonstrates a strong resistance to anticancer drugs, presumably due to its resistance to drug induced apoptosis. Although gemcitabine (GEM) might be partially effective for treating advanced pancreatic cancer, its efficacy is still less than satisfactory. Galectin-3 (gal-3), a member of the β-galact...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-011-9376-x
更新日期：2011-04-01 00:00:00
abstract：:Tumor cells exposed to a growth stress such as low pH, glucose starvation and hypoxia have been shown to exhibit a transient increase in experimental metastatic potential, particularly when allowed to recover under normal growth conditions for a period of 24-48 h. In this study we examined whether this increase in met...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1018470709523
更新日期：1997-09-01 00:00:00
abstract：:To elucidate the development of bone metastasis in human neuroblastoma, bone marrow and bone metastases were analysed histologically in a hematogeneous metastasis model of murine neuroblastoma. The bone marrow metastasis occurred initially in the bone marrow sinusoid where tumor cells adhered and extravasated to bone ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00128962
更新日期：1996-10-01 00:00:00
abstract：:The effects of gamma-irradiation on the properties of microvessel endothelial cells were studied in vitro. After incubating confluent endothelial cell monolayers in low serum-containing medium for 24 h, the monolayers were irradiated with 137Cs. Survival of rat lung microvessel endothelial (RLE) and mouse brain microv...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01785531
更新日期：1991-09-01 00:00:00
