Abstract：
:Several lines of evidence indicate that sialosyl Le(a), tumor-associated carbohydrate antigen present on human colon carcinoma cells, is involved in formation of metastases. To study the role of this carbohydrate structure in development of metastases, we have used the clone of human colon carcinoma CX-1 cells transfected with antisense expression vector containing fragment of cDNA for alpha1,3/4-fucosyltransferase (FT III), which is involved in synthesis of sialosyl Le(a) tetrasaccharide. It has been reported previously that, in contrast to the parental cells, the antisense-transfected CX-1.1AS5 cells do not express sialosyl Le(a) and do not adhere to E-selectin-expressing CHO cells. In the present work we have studied the formation of liver metastases by CX-1.1AS5 cells after their orthotopic or intrasplenic implantation into athymic nu/nu mice. After orthotopic implantation of sialosyl Le(a)-negative colon carcinoma CX-1.1AS5 cells, the number of mice with liver metastases was markedly lower (21% of mice) in comparison with their number after implantation of the parental CX-1.1 cells (86% of mice). However, no differences in ability to form colonies in liver were observed between parental CX-1.1 cells and antisense-transfected CX-1.1AS5 cells after intrasplenic inoculation. The liver metastases were formed in 89% and 84% of mice, respectively. Our data support the thesis on the importance of sialosyl Le(a) antigen expression in the development of liver metastases by colon cancer cells, and indicate the role of transplantation route and primary tumor localization in formation of metastases.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Opolski A,Laskowska A,Madej J,Wietrzyk J,Kłopocki A,Radzikowski C,Ugorski Mdoi
10.1023/a:1006502009682subject
Has Abstractpub_date
1998-11-01 00:00:00pages
673-81issue
8eissn
0262-0898issn
1573-7276journal_volume
16pub_type
杂志文章abstract：:Although the loss of sulfomucins was known as an indicator of carcinogenesis and malignant progression of colonic epithelia, it was not known whether the loss was directly related to the malignant behavior of colon carcinoma cells. We have studied the biological properties of LS174T human colon carcinoma cells before ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006654027742
更新日期：1999-03-01 00:00:00
abstract：:Breast cancer has a predilection for metastasis to the bone marrow. The preprotachykinin-I (PPT-I) gene has a central role in the early migration of breast cancer cells into the bone marrow, making this organ a latent repository of the cancer cells. This study investigated whether the invasive and metastatic potential...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-006-9001-6
更新日期：2005-01-01 00:00:00
abstract：:We recently developed a method for the isolation and purification of tumour-derived endothelium. In this study the phenotypic and functional properties of human tumour-derived microvascular endothelial cells (TdMEC) were examined. Endothelium obtained from human adrenal gland specimens (HAMEC) was used as a reference ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006738901839
更新日期：1999-01-01 00:00:00
abstract：:The effects of treatment of human lung carcinoma cell line A 549 with recombinant DNA-derived human leukocyte interferons A (rIFN-alpha A) or D (rIFN-alpha D), and human lymphoblastoid interferon (Wellferon) on in vitro cell invasion were investigated in a quantitative invasion assay using human amnion. The A 549 cell...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00117932
更新日期：1986-07-01 00:00:00
abstract：:RGD-containing substrates were used to study static and hydrodynamic adhesion of murine RAW117 large-cell lymphoma sublines with differential liver-metastatic potentials. Highly liver-metastatic RAW117-H10 cells had higher rates of static adhesion to vitronectin, fibronectin and (GRGDS)4 than poorly metastatic RAW117-...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1018451616309
更新日期：1997-01-01 00:00:00
abstract：:The mechanisms leading to rapid invasive growth of malignant gliomas are poorly understood. Expression of the hyaluronic acid (HA) receptor CD44 and adhesion to HA are involved in invasive properties. Our previous studies have shown that malignant glioma cells are able to adhere to extracellular HA. Here we investigat...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1026425519497
更新日期：1999-02-01 00:00:00
abstract：:In breast tumors, high levels of leptin have been associated with increased incidence of breast cancer metastasis. Breast cancer metastasis is directly associated with breast cancer cell invasion. However, whether leptin could augment breast cancer cell invasion is not known. Here we showed that leptin increased the i...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-008-9231-x
更新日期：2009-01-01 00:00:00
abstract：:The major obstacle to devising effective ways to treat cancer is its heterogeneity and genetic instability. It was originally postulated that targeting the process of tumor angiogenesis could circumvent this problem, as it involves genetically stable epigenetically controlled host stroma. Thus, anti-angiogenic approac...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1023/a:1022935117193
更新日期：2003-01-01 00:00:00
abstract：:Primary brain tumors lack the metastatic behavior that is in part believed to be promoted by the extracellular matrix (ECM) components of the basement membrane. This study was intended to examine the influence of the ECM components present in the basement membrane that may act as natural barriers to tumor cell invasio...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00123395
更新日期：1996-09-01 00:00:00
abstract：:We have previously reported that transfection of mouse interferon gamma (IFN-gamma) in H-2Kk-positive BW variants (BW-Sp3) abolishes tumorigenicity and reduces metastatic capacity. To further increase the immunogenicity of BW-Sp3 cells, the gene for human interleukin 2 (huIL-2) was transfected in these cancer cells. S...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006585525399
更新日期：1998-07-01 00:00:00
abstract：PURPOSE:To evaluate lymphangiogenesis in patients with breast carcinoma, explore the underlying mechanism, and study the relationship between lymphangiogenesis and progression of breast carcinoma. METHODS:Sixty-one cases of breast carcinoma with complete clinical and pathological data were analyzed. Using an anti-podo...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-008-9180-4
更新日期：2008-01-01 00:00:00
abstract：:We have developed a novel hyperthermic treatment modality using magnetic materials for metastatic bone tumors. The purpose of this study is to show the results of novel hyperthermia for metastatic bone tumors. This novel hyperthermic treatment modality was used for 15 patients with 16 metastatic bone lesions. In seven...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9068-8
更新日期：2007-01-01 00:00:00
abstract：:Cancer cells with the surface marker profile CD44+/CD24- have previously been described to possess cancer stem cell-like properties. This manuscript evaluates those properties in ovarian cancer cell lines. The proportion of CD44+/CD24- cells corresponded to the clinical aggressiveness of each ovarian cancer cell line ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9482-4
更新日期：2012-12-01 00:00:00
abstract：:Primary tumor growth and metastasis depend on angiogenesis, which is determined by the balance between proangiogenic and antiangiogenic molecules. Interferon (IFN)-alpha and -beta inhibit angiogenesis through downregulation of interleukin-8, matrix metalloproteinase-9, and basic fibroblast growth factor. To provide ev...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1020923326441
更新日期：2002-01-01 00:00:00
abstract：:Circulating microRNAs have been described as head and neck cancer biomarkers in multiple anatomical subsites including the oral cavity, nasopharynx, larynx, salivary glands and the skin. While there is an expanding volume of published literature showing the significance of individual or panels of microRNAs, the clinic...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-019-09961-6
更新日期：2019-06-01 00:00:00
abstract：:Cancer metastasis shows great diversity in target organs, routes and molecular mechanisms depending on the type of cancer and even on the individual patients. To identify key molecules involved in metastasis, we constructed a murine model system including multiple sublines with different organotropism and pathways of ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-019-09979-w
更新日期：2019-08-01 00:00:00
abstract：:Lung cancer is the most frequent and one of the most deadly cancer types and is classified into small cell lung cancer and non-small cell lung cancer (NSCLC). Transforming growth factor beta (TGFβ) regulates a wide array of cell functions and plays a major role in lung diseases, including NSCLC. TGFβ signals through t...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9598-1
更新日期：2013-12-01 00:00:00
abstract：:To analyze the prognostic value of the extent of peritumoral brain edema in patients operated for single brain metastases (BM), we retrospectively evaluated pre-operative magnetic resonance images in a discovery cohort of 129 patients and a validation cohort of 118 patients, who underwent neurosurgical resection of a ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9542-9
更新日期：2013-04-01 00:00:00
abstract：:The interaction between circulating tumor cells and blood components, mainly platelets, plays an important role during metastasis. In this study, we prepared liposomes containing the platelet aggregation inhibitor Cilostazol (Cil-L). The objective of this study was to investigate the effect of this Cil-L on platelet a...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-009-9299-y
更新日期：2010-01-01 00:00:00
abstract：:The idea for this Special Issue originated from our recent review in Nature Reviews Clinical Oncology entitled "Does the mobilization of circulating tumour cells during cancer therapy cause metastasis?" Martin et al. (Nat Rev Clin Oncol 14:32-44, 2017). While preparing this review, it became evident that an overwhelmi...
journal_title：Clinical & experimental metastasis
pub_type： 社论
doi：10.1007/s10585-018-9914-x
更新日期：2018-04-01 00:00:00
abstract：:The cancer preventive properties of grape products such as red wine have been attributed to polyphenols enriched in red wine. However, much of the studies on cancer preventive mechanisms of grape polyphenols have been conducted with individual compounds at concentrations too high to be achieved via dietary consumption...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-009-9250-2
更新日期：2009-01-01 00:00:00
abstract：:Immunotherapy by vaccination represents a novel method for treatment of cancer. In this regard, vaccines with the broadest possible menu of relevant antigens stand the greatest chance of success. Tissue vaccines are composed of material harvested directly from tumors and contain not only antigens associated with neopl...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-008-9213-z
更新日期：2008-01-01 00:00:00
abstract：:Extrathyroidal extension (ETE) of papillary thyroid carcinoma (PTC) is common and clinical presentation can vary from minimal to extensive locoregional involvement. Although PTC is generally considered the most benign among all thyroid carcinomas, it may present with local invasion with poor prognosis. Our retrospecti...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9800-3
更新日期：2016-08-01 00:00:00
abstract：:About 30-40% of patients with renal cell carcinoma (RCC) will develop metastasis after curative nephrectomy. There is a strong need to identify the early metastasis with conventional and molecular risk factors. The present study aimed to test if analysis of the CA9 gene can provide useful information to predict early ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9064-z
更新日期：2007-01-01 00:00:00
abstract：:Human CD44 standard isoform (hCD44s) cDNA regulated by a high-expressing promoter was transfected into Balb/c 3T3 cells and the tumorigenic and metastatic capacities of the transfectants were investigated in nude mice at the subcutaneous site. One of three transfectants was tumorigenic. hCD44s expression was lost in t...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006568103588
更新日期：1998-01-01 00:00:00
abstract：:We have used 5'-deoxy-5'-S isobutyl-thioadenosine (SIBA), an analog of S-adenosylhomocysteine, alone or in association with a methionine-depleted diet in order to obtain an antitumoral effect in two different tumor models: a transplantable rat rhabdomyosarcoma (RMS-J1) induced by i.m. injection of nickel and the well-...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01580402
更新日期：1988-01-01 00:00:00
abstract：:Metastasis is key to cancer mortality. Understanding its biology is vital for developing strategies to prevent and treat metastasis. Phenotypic assays to either study metastasis or evaluate anti-metastatic drugs are widely used in preclinical research. This technical note discusses the adherence of reporting essential...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-018-9944-4
更新日期：2018-12-01 00:00:00
abstract：:Blood samples, bone marrow, tumours and metastases where possible were collected from SCID mice bearing orthotopic xenografts of the triple-negative MDA-MB-468 cell line or a transplantable ER-positive patient derived xenograft (ED-03), and assessed using human-specific, tandem-nested RT-qPCR for markers relating to d...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-019-09977-y
更新日期：2019-08-01 00:00:00
abstract：:Clear cell renal cell carcinoma (ccRCC) is one of few cancers with rising incidence in North America. The prognosis of ccRCC is variable and difficult to predict. Stratification of patients according to disease aggressiveness can significantly improve patient management. We investigated the expression of the S100A11 p...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-015-9758-6
更新日期：2016-01-01 00:00:00
abstract：:Anti-resorptive bisphosphonates (BPs) have been clinically used to prevent cancer-bone metastasis and cancer-induced bone pathologies despite the fact that the phenotypic response of the cancer-bone interactions to BP exposure is "uncharted territory". This study offers unique insights into the interplay between cance...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9798-6
更新日期：2016-08-01 00:00:00
