Abstract：
:Although the loss of sulfomucins was known as an indicator of carcinogenesis and malignant progression of colonic epithelia, it was not known whether the loss was directly related to the malignant behavior of colon carcinoma cells. We have studied the biological properties of LS174T human colon carcinoma cells before and after suppression of sulfomucin production. Incorporation of [35S]-sulfate into high molecular weight mucins decreased after carcinoma cell treatment with 1.5% dimethylsulfoxide (DMSO) for 8 days. The amounts of sulfomucin determined using a sulfomucin-specific monoclonal antibody (mAb 91.9H), in Western blot and flowcytometric analyses, also decreased. In addition, the levels of MUC2 and MUC5B mucin gene expression measured by RT-PCR were reduced after DMSO-treatment, whereas the levels of MUC1, MUC5AC, and MUC6 mucin gene expression were not. The DMSO-treated cells were tested in vitro and in vivo for their properties. Differences were not detected in their anchorage-independent growth, anchorage-dependent growth, E-selectin-dependent cell adhesion or sensitivity to interleukin (IL)-2-activated lymphocyte cytolysis. When untreated or DMSO-treated LS174T cells were injected intrasplenically into nude mice, the treated cells lacking certain cell surface sulfomucins formed fewer metastatic colonies in the liver. These results suggest that the loss of sulfomucins by colonic epithelial cells during progression is not directly related to the enhanced malignant behavior.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Tsuiji H,Nakatsugawa S,Ishigaki T,Irimura Tdoi
10.1023/a:1006654027742subject
Has Abstractpub_date
1999-03-01 00:00:00pages
97-104issue
2eissn
0262-0898issn
1573-7276journal_volume
17pub_type
杂志文章abstract：:For more effective oncological management of disseminated colorectal cancer, therapies must be devised that target the different individual stages of metastasis development. Recent work showed that integrin subunits alpha2, alpha6 and beta4 are involved in the colorectal cancer cell extravasation process. By means of ...
journal_title：Clinical & experimental metastasis
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journal_title：Clinical & experimental metastasis
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journal_title：Clinical & experimental metastasis
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journal_title：Clinical & experimental metastasis
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journal_title：Clinical & experimental metastasis
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journal_title：Clinical & experimental metastasis
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
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journal_title：Clinical & experimental metastasis
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journal_title：Clinical & experimental metastasis
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
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更新日期：1998-11-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00128962
更新日期：1996-10-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
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更新日期：2004-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9482-4
更新日期：2012-12-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
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更新日期：2000-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01753663
更新日期：1989-07-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00058058
更新日期：1987-04-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
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更新日期：2015-02-01 00:00:00
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journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
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更新日期：2004-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
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journal_title：Clinical & experimental metastasis
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journal_title：Clinical & experimental metastasis
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journal_title：Clinical & experimental metastasis
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更新日期：1999-03-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2005-01-01 00:00:00
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journal_title：Clinical & experimental metastasis
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更新日期：2012-10-01 00:00:00
