Malignant and other properties of human colon carcinoma cells after suppression of sulfomucin production in vitro.


:Although the loss of sulfomucins was known as an indicator of carcinogenesis and malignant progression of colonic epithelia, it was not known whether the loss was directly related to the malignant behavior of colon carcinoma cells. We have studied the biological properties of LS174T human colon carcinoma cells before and after suppression of sulfomucin production. Incorporation of [35S]-sulfate into high molecular weight mucins decreased after carcinoma cell treatment with 1.5% dimethylsulfoxide (DMSO) for 8 days. The amounts of sulfomucin determined using a sulfomucin-specific monoclonal antibody (mAb 91.9H), in Western blot and flowcytometric analyses, also decreased. In addition, the levels of MUC2 and MUC5B mucin gene expression measured by RT-PCR were reduced after DMSO-treatment, whereas the levels of MUC1, MUC5AC, and MUC6 mucin gene expression were not. The DMSO-treated cells were tested in vitro and in vivo for their properties. Differences were not detected in their anchorage-independent growth, anchorage-dependent growth, E-selectin-dependent cell adhesion or sensitivity to interleukin (IL)-2-activated lymphocyte cytolysis. When untreated or DMSO-treated LS174T cells were injected intrasplenically into nude mice, the treated cells lacking certain cell surface sulfomucins formed fewer metastatic colonies in the liver. These results suggest that the loss of sulfomucins by colonic epithelial cells during progression is not directly related to the enhanced malignant behavior.


Clin Exp Metastasis


Tsuiji H,Nakatsugawa S,Ishigaki T,Irimura T




Has Abstract


1999-03-01 00:00:00












  • Parathyroid hormone-related protein and ezrin are up-regulated in human lung cancer bone metastases.

    abstract::Lung cancer often metastasizes to bone in patients with advanced disease. Identification of the factors involved in the interactions between lung cancer cells and bone will improve the prevention and treatment of bone metastases. We identified changes in metastasis-related gene expression of human HARA lung squamous c...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Deng X,Tannehill-Gregg SH,Nadella MV,He G,Levine A,Cao Y,Rosol TJ

    更新日期:2007-01-01 00:00:00

  • Differential growth and responsiveness to cancer therapy of tumor cells in different environments.

    abstract::Tumor metastasis often confers poor prognosis for cancer patients due to lack of comprehensive strategy in dealing with cells growing in different environment. Current anticancer therapies have incomplete effectiveness because they were designed assuming metastatic tumors behave similarly in different organs. We hypot...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Alsaggar M,Yao Q,Cai H,Liu D

    更新日期:2016-02-01 00:00:00

  • Mitogen activated protein kinase pathway is involved in RhoC GTPase induced motility, invasion and angiogenesis in inflammatory breast cancer.

    abstract::Inflammatory breast cancer (IBC) is the most lethal form of locally advanced breast cancer known. IBC carries a guarded prognosis primarily due to rapid onset of disease, typically within six months, and the propensity of tumor emboli to invade the dermal lymphatics and spread systemically. Although the clinical manif...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: van Golen KL,Bao LW,Pan Q,Miller FR,Wu ZF,Merajver SD

    更新日期:2002-01-01 00:00:00

  • Modulation of clonal progression in B16F1 melanoma cells.

    abstract::We have examined the effects of the microenvironment on the frequency and generation of metastatic variant cells in both parental B16F1 melanoma cells and nascent clones. The metastatic abilities of cultured B16F1 cells were tested after a period of growth in the presence or absence of a second cell population separat...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Harris JF,Chambers AF,Tam AS

    更新日期:1991-03-01 00:00:00

  • Oral administration of muscle derived small molecules inhibits tumor spread while promoting normal cell growth in mice.

    abstract::Tumor metastases are extremely rare in striated muscles. This is surprising given the fact that this tissue constitutes 60% of body weight. The present study focuses on small molecules produced and secreted by muscle cells which possess anti-cancer activity in vivo. Recently we have shown that a low molecular weight f...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Bar-Yehuda S,Farbstein T,Barer F,Ohana G,Fishman P

    更新日期:1999-01-01 00:00:00

  • Inhibitory effect of non-anticoagulant heparin (S-NACH) on pancreatic cancer cell adhesion and metastasis in human umbilical cord vessel segment and in mouse model.

    abstract::Metastasis is the most devastating aspect of cancer and it is the main cause of morbidity and mortality in cancer patients. Tumor cell adhesion to the vascular endothelial cell lining is an important step in metastatic progression and is prompted by platelets. Mucin 1 is over-expressed and aberrantly glycosylated in m...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Sudha T,Phillips P,Kanaan C,Linhardt RJ,Borsig L,Mousa SA

    更新日期:2012-06-01 00:00:00

  • A novel in vitro assay system for transendothelial tumor cell invasion: significance of E-selectin and alpha 3 integrin in the transendothelial invasion by HT1080 fibrosarcoma cells.

    abstract::The interaction of tumor cells with endothelial cells is a key event in tumor metastasis. We established an in vitro invasion assay system, in which the invasion of tumor cells after interaction with endothelial cells can be examined. Two chamber culture wells separated by porous membrane were used. Human umbilical ve...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Okada T,Okuno H,Mitsui Y

    更新日期:1994-07-01 00:00:00

  • Estradiol increases ER-negative breast cancer metastasis in an experimental model.

    abstract::Breast cancer (BC) is the most common cancer affecting women in the United States and metastatic breast cancer is the leading cause of death. The role estradiol plays in ER-positive BC is well-documented, but the way it contributes to ER-negative BC remains unclear. In the present study, we utilized an experimental mo...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Yang X,Belosay A,Du M,Fan TM,Turner RT,Iwaniec UT,Helferich WG

    更新日期:2013-08-01 00:00:00

  • Assessment of methylation status of locoregional lymph nodes in lung cancer using EBUS-NA.

    abstract::Hypermethylation of the promoter region of tumor suppressor genes is associated with carcinogenesis in lung cancer (LC). Endobronchial ultrasound with needle aspiration (EBUS-NA) is a semi-invasive method for obtaining cell blocks from lymph nodes, which can be used for epigenetic analyses. To establish the relationsh...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Millares L,Serra M,Andreo F,Sanz-Santos J,Montón C,Grimau C,Gallego M,Setó L,Combalia N,Llatjos M,Escoda R,Castellà E,Monsó E

    更新日期:2015-10-01 00:00:00

  • Plasma basic fibroblast growth factor levels in colorectal cancer: a clinically useful assay?

    abstract::Angiogenic cytokines in the plasma and serum of cancer patients may serve as 'surrogate' markers of tumour neoangiogenesis. Serum VEGF correlates with disease stage in colorectal cancer (CRC), but the role of bFGF in CRC is uncertain. This study aimed to assess plasma bFGF levels in CRC patients before treatment, duri...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: George ML,Tutton MG,Abulafi AM,Eccles SA,Swift RI

    更新日期:2002-01-01 00:00:00

  • Increased lymphangiogenesis in lung metastases from colorectal cancer is associated with early lymph node recurrence and decreased overall survival.

    abstract::Pulmonary metastasectomy (PM) is an accepted treatment modality in colorectal cancer (CRC) patients with pulmonary tumor spread. Positive intrathoracic lymph nodes at the time of PM are associated with a poor prognosis and 5-year survival rates of <20 %. Increased lymphangiogenesis in pulmonary metastases might repres...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,多中心研究


    authors: Schweiger T,Nikolowsky C,Graeter T,Seebacher G,Laufer J,Glueck O,Glogner C,Birner P,Lang G,Klepetko W,Ankersmit HJ,Hoetzenecker K

    更新日期:2016-02-01 00:00:00

  • Isolation and characterization of a low metastatic variant from EL-4 mouse T-lymphoma.

    abstract::A variant cell line (EL-4ad) which adhered to a tissue culture dish was isolated from highly metastatic EL-4 murine T-lymphoma. The experimental and spontaneous metastatic ability of EL-4ad was lower than that of the EL-4 parent cell line. The cell surface phenotypes of both cell lines were CD2+3+4-8-45+TCR alpha beta...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Ota T,Tanino M,Kohno H,Funamoto H,Odashima S

    更新日期:1992-09-01 00:00:00

  • Sialylation of E-cadherin does not change the spontaneous or ET-18-OMe-mediated aggregation of MCF-7 human breast cancer cells.

    abstract::We have investigated the role of sialylation on cell-cell adhesion mediated by E-cadherin. Two MCF-7 human breast cancer cell variants were studied: MCF-7/AZ cells showed a spontaneous cell-cell adhesion in the fast and slow aggregation assay. whereas the adhesion deficient MCF-7/6 cell variant failed to form larger a...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Steelant WF,Recchi MA,Noë VT,Boilly-Marer Y,Bruyneel EA,Verbert A,Mareel MM,Delannoy P

    更新日期:1999-05-01 00:00:00

  • Transcriptional profiling identifies genes induced by hepatocyte-derived extracellular matrix in metastatic human colorectal cancer cell lines.

    abstract::The milieu of the liver, and in particular hepatocyte-derived extracellular matrix (hECM), is a critical factor regulating development of liver metastases of colorectal cancer (CRC) cells. The present study has investigated genes altered by hECM in CRC cells and particularly by heparan sulfate chains of hepatocyte pro...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Zvibel I,Wagner A,Pasmanik-Chor M,Varol C,Oron-Karni V,Santo EM,Halpern Z,Kariv R

    更新日期:2013-02-01 00:00:00

  • TGF-beta specifically enhances the metastatic attributes of murine lung adenocarcinoma: implications for human non-small cell lung cancer.

    abstract::Lung cancer is the most frequent and one of the most deadly cancer types and is classified into small cell lung cancer and non-small cell lung cancer (NSCLC). Transforming growth factor beta (TGFβ) regulates a wide array of cell functions and plays a major role in lung diseases, including NSCLC. TGFβ signals through t...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Vázquez PF,Carlini MJ,Daroqui MC,Colombo L,Dalurzo ML,Smith DE,Grasselli J,Pallotta MG,Ehrlich M,Bal de Kier Joffé ED,Puricelli L

    更新日期:2013-12-01 00:00:00

  • Identification of S100A14 as a metastasis-promoting molecule in a murine organotropic metastasis model.

    abstract::Cancer metastasis shows great diversity in target organs, routes and molecular mechanisms depending on the type of cancer and even on the individual patients. To identify key molecules involved in metastasis, we constructed a murine model system including multiple sublines with different organotropism and pathways of ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Sugino T,Ichikawa-Tomikawa N,Tanaka M,Shishito N,Miura T,Abe M,Muramatsu K,Oishi T,Kakuda Y,Kawata T,Akiyama Y

    更新日期:2019-08-01 00:00:00

  • Is adjuvant treatment with vinblastine effective in reducing the occurrence of distant metastasis in limited squamous cell lung cancer? A randomized study.

    abstract::In order to study the usefulness of treatment with vinblastine (VLB) in the prevention of cancer metastasis in squamous cell lung cancer, 50 patients with locoregional disease were randomized to receive either locoregional RT alone (group A) or a weekly intravenous bolus injection of VLB (6 mg/m2) concurrently with an...

    journal_title:Clinical & experimental metastasis

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Schallier DC,De Neve WJ,De Greve JL,Van Belle SP,De Wasch GJ,Dotremont G,Storme GA

    更新日期:1988-01-01 00:00:00

  • Molecular phenotype is associated with survival in breast cancer patients with spinal bone metastases.

    abstract::To aid in therapy selection for patients with spinal bone metastases (SBM), predictive models have been developed. These models consider SBM from breast cancer a positive predictive factor, but do not take phenotypes based on estrogen (ER), progesterone (PR) and human epidermal growth factor 2 (HER2) receptors into ac...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,多中心研究


    authors: Bollen L,Wibmer C,Wang M,van der Linden YM,Leithner A,Bünger CE,Jensen AB,Fiocco M,Bratschitsch G,Pondaag W,Bovée JV,Dijkstra PD

    更新日期:2015-01-01 00:00:00

  • Transcriptional regulation of osteopontin and the metastatic phenotype: evidence for a Ras-activated enhancer in the human OPN promoter.

    abstract::Elevated osteopontin (OPN) transcription often correlates with increased metastatic potential of transformed cells, and in several model systems OPN--whether produced by the tumor cells or by stromal cells - has been shown to enhance metastatic ability. Sequence elements in the OPN promoter have been identified on the...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Denhardt DT,Mistretta D,Chambers AF,Krishna S,Porter JF,Raghuram S,Rittling SR

    更新日期:2003-01-01 00:00:00

  • Transient silencing of galectin-3 expression promotes both in vitro and in vivo drug-induced apoptosis of human pancreatic carcinoma cells.

    abstract::Pancreatic cancer demonstrates a strong resistance to anticancer drugs, presumably due to its resistance to drug induced apoptosis. Although gemcitabine (GEM) might be partially effective for treating advanced pancreatic cancer, its efficacy is still less than satisfactory. Galectin-3 (gal-3), a member of the β-galact...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Kobayashi T,Shimura T,Yajima T,Kubo N,Araki K,Wada W,Tsutsumi S,Suzuki H,Kuwano H,Raz A

    更新日期:2011-04-01 00:00:00

  • Expression of activated gelatinase in human invasive breast carcinoma.

    abstract::The expression of both 92- and 72-kDa gelatinases has been studied in 20 samples of human breast carcinoma by the technique of gelatin zymography. This technique allowed the relative amount of each gelatinase to be determined in small samples of tissue (< 10 mg). More importantly, active and latent forms of the two ge...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Brown PD,Bloxidge RE,Anderson E,Howell A

    更新日期:1993-03-01 00:00:00

  • Treatment with human recombinant leukocyte interferons inhibits in vitro invasive ability of human lung carcinoma cells.

    abstract::The effects of treatment of human lung carcinoma cell line A 549 with recombinant DNA-derived human leukocyte interferons A (rIFN-alpha A) or D (rIFN-alpha D), and human lymphoblastoid interferon (Wellferon) on in vitro cell invasion were investigated in a quantitative invasion assay using human amnion. The A 549 cell...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Ravine TJ,Ledinko N

    更新日期:1986-07-01 00:00:00

  • Experimental liver metastasis: standards for local cell implantation to study isolated tumor growth in mice.

    abstract::Experimental hepatic metastasis of colorectal tumors is frequently studied by local intrahepatic tumor cell implantation. However, although a variety of factors of the implantation procedure may markedly influence tumor growth characteristics, standards are not defined yet. Herein, we studied the effect of different m...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Kollmar O,Schilling MK,Menger MD

    更新日期:2004-01-01 00:00:00

  • Control pathways of the 67 kDa laminin binding protein: surface expression and activity of a new ligand binding domain.

    abstract::A number of papers have been published on the clinical correlation of the expression of the 67 kDa laminin binding protein (LBP) with the metastatic potential of solid tumors. Both mRNA and protein expression levels have been reported, but both the relationship between them and the molecular nature of the 67 kDa surfa...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Landowski TH,Uthayakumar S,Starkey JR

    更新日期:1995-09-01 00:00:00

  • Gene transduction of NK4, HGF antagonist, inhibits in vitro invasion and in vivo growth of human pancreatic cancer.

    abstract::In this study, we investigated the therapeutic effects of adenovirally-mediated transfer of the sequence of NK4, an antagonist for hepatocyte growth factor (HGF), against human pancreatic carcinoma. HGF has been implicated to play an important role in invasion and metastasis of various human cancers through tumor-stro...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Maehara N,Nagai E,Mizumoto K,Sato N,Matsumoto K,Nakamura T,Narumi K,Nukiwa T,Tanaka M

    更新日期:2002-01-01 00:00:00

  • Cancer initiation and progression within the cancer microenvironment.

    abstract::Within the cancer microenvironment, the growth and proliferation of cancer cells in the primary site as well as in the metastatic site represent a global biological phenomenon. To understand the growth, proliferation and progression of cancer either by local expansion and/or metastasis, it is important to understand t...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Leong SP,Aktipis A,Maley C

    更新日期:2018-08-01 00:00:00

  • Time to metastatic relapse and breast cancer cells dissemination in bone marrow at metastatic relapse.

    abstract::Breast cancer dissemination can be monitored in patients by detecting circulating and/or disseminated tumor cells. However, bone marrow disseminated tumor cells (BM DTC) may undergo a dormancy during several years before growing (or not) into clinically detectable metastases. We therefore hypothesized that breast canc...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Bidard FC,Vincent-Salomon A,Sigal-Zafrani B,Rodrigues M,Diéras V,Mignot L,Sastre-Garau X,Poupon MF,Pierga JY

    更新日期:2008-01-01 00:00:00

  • Is the Fischer 344/CRJ rat a protein-knock-out model for dipeptidyl peptidase IV-mediated lung metastasis of breast cancer?

    abstract::Fischer 344/CRJ rats harbor a G633R substitution in dipeptidyl peptidase IV (DPP IV) that leads to retention and degradation of the mutant protein in the endoplasmic reticulum (Tsuji E, Misumi Y, Fujiwara T et al. Biochemistry 1992; 31 (47): 11921-7). However, when these rats were used as a 'protein knock-out' model i...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Cheng HC,Abdel-Ghany M,Zhang S,Pauli BU

    更新日期:1999-01-01 00:00:00

  • MTBP inhibits migration and metastasis of hepatocellular carcinoma.

    abstract::Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide with increasing incidence. Despite curative surgical resection and advanced chemotherapy, its survival rate remains low. The presence of microvascular invasion and occult metastasis is one of the major causes for this poor outcome. MDM2 Binding...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Bi Q,Ranjan A,Fan R,Agarwal N,Welch DR,Weinman SA,Ding J,Iwakuma T

    更新日期:2015-04-01 00:00:00

  • Syndecan-1: a dynamic regulator of the myeloma microenvironment.

    abstract::Emerging data in myeloma and other cancers indicates that heparan sulfate proteoglycans promote tumor progression by enhancing their growth and metastasis. By acting as key regulators of cell signaling via their interactions with multiple growth and angiogenic factors, heparan sulfates mediate a shift in the microenvi...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Sanderson RD,Yang Y

    更新日期:2008-01-01 00:00:00