Abstract:
:It is recognised that stromal cells determine cancer progression. We have previously shown that active TGFbeta produced by rat colon carcinoma cells modulated NO production in rat endothelial cells. To elucidate the role of TGFbeta and NO in the mechanisms of interaction of colon carcinoma cells with stromal cells and in cancer progression, we transfected REGb cells, a regressive colon carcinoma clone secreting latent TGFbeta, with a cDNA encoding for a constitutively-secreted active TGFbeta. Out of 20 injected rats only one tumour progressed, which was resected and sub-cultured (ReBeta cells). ReBeta cells secreted high levels of active TGFbeta. The adhesive properties of REGb and Rebeta cells to endothelial cells were similar, showing that the secretion of active TGFbeta is not involved in tumour cell adhesion to endothelial cells. ReBeta, but not REGb, cell culture supernatants inhibited cytokine-dependent NO secretion by endothelial cells, but inhibition of NO production was similar in co-cultures of REGb or ReBeta cells with endothelial cells. Therefore, secretion of active TGFbeta regulated endothelial NO synthase activity when tumour cells were distant from, but not in direct contact with, endothelial cells. However, only ReBeta cells inhibited cytokine-dependent secretion of NO in coculture with macrophages, indicating that the active-TGFbeta-NO axis confers an advantage for tumour cells in their interaction with macrophages rather than endothelial cells in cancer progression.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Lohm S,Peduto-Eberl L,Lagadec P,Renggli-Zulliger N,Dudler J,Jeannin JF,Juillerat-Jeanneret Ldoi
10.1007/s10585-005-0431-3subject
Has Abstractpub_date
2005-01-01 00:00:00pages
341-9issue
4eissn
0262-0898issn
1573-7276journal_volume
22pub_type
杂志文章abstract::We have examined the role of platelet-endothelial cell adhesion molecule-1 (PECAM-1/CD31) during the transendothelial migration of melanoma cells using a novel in vitro system. Comparable studies have suggested the involvement of PECAM-1 in leukocyte transendothelial migration. Such studies have been confirmed using i...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
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journal_title:Clinical & experimental metastasis
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journal_title:Clinical & experimental metastasis
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-004-2696-3
更新日期:2004-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
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journal_title:Clinical & experimental metastasis
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更新日期:2013-04-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章,meta分析,评审
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journal_title:Clinical & experimental metastasis
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journal_title:Clinical & experimental metastasis
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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pub_type: 杂志文章
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journal_title:Clinical & experimental metastasis
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更新日期:2013-04-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
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更新日期:2000-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 临床试验,杂志文章
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更新日期:1997-07-01 00:00:00
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doi:10.1023/a:1013849123736
更新日期:2002-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1021322201816
更新日期:2002-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
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更新日期:2011-04-01 00:00:00
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journal_title:Clinical & experimental metastasis
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更新日期:2008-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1006649617918
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1006540824969
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章,评审
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更新日期:2008-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF02057179
更新日期:1989-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF00123395
更新日期:1996-09-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-006-9034-x
更新日期:2006-01-01 00:00:00
abstract::About 30-40% of patients with renal cell carcinoma (RCC) will develop metastasis after curative nephrectomy. There is a strong need to identify the early metastasis with conventional and molecular risk factors. The present study aimed to test if analysis of the CA9 gene can provide useful information to predict early ...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-007-9064-z
更新日期:2007-01-01 00:00:00
abstract::The effects of the cyclooxygenase (COX)-2 inhibitor nimesulide on bombesin-enhanced peritoneal metastasis of azoxymethane (AOM)-induced intestinal adenocarcinomas were investigated in male Wistar rats. From the beginning of the study, the rats were given 10 weekly s.c. injections of AOM (7.4 mg/kg body weight) and s.c...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1025883129932
更新日期:2003-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 临床试验,杂志文章
doi:10.1023/a:1010991213842
更新日期:2000-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-008-9213-z
更新日期:2008-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-009-9235-1
更新日期:2009-01-01 00:00:00