Transient silencing of galectin-3 expression promotes both in vitro and in vivo drug-induced apoptosis of human pancreatic carcinoma cells.


:Pancreatic cancer demonstrates a strong resistance to anticancer drugs, presumably due to its resistance to drug induced apoptosis. Although gemcitabine (GEM) might be partially effective for treating advanced pancreatic cancer, its efficacy is still less than satisfactory. Galectin-3 (gal-3), a member of the β-galactoside-binding protein family, is a multifunctional protein with roles in tumor cell adhesion, proliferation, differentiation, angiogenesis, metastasis, and apoptosis. We have utilized gal-3 small interfering RNA (siRNA) to probe whether gal-3 regulates anticancer drug-induced apoptosis in pancreatic cancer cells. We found that Gal-3 siRNA augmented GEM- and cisplatin-induced apoptosis in pancreatic cancer cell lines in vitro. Mitochondrial depolarization induction was increased in gal-3-silenced cells after GEM treatment, resulting in activation of caspase-9, but not caspase-8. Akt phosphorylation was significantly downregulated in gal-3- silenced cells in association with apoptosis. Moreover, intratumoral administration of gal-3 siRNA increased the GEM sensitivity of tumor xenografts produced by subcutaneous inoculation of pancreatic cancer cells into nude mice. These results suggest that gal-3 might provide a novel therapeutic target in pancreatic cancer.


Clin Exp Metastasis


Kobayashi T,Shimura T,Yajima T,Kubo N,Araki K,Wada W,Tsutsumi S,Suzuki H,Kuwano H,Raz A




Has Abstract


2011-04-01 00:00:00












  • Anti-metastatic efficacy and safety of MMI-166, a selective matrix metalloproteinase inhibitor.

    abstract::The anti-metastatic efficacy and safety of a newly-developed matrix metalloproteinase (MMP) inhibitor were examined. MMI-166, a N-sulfonylamino acid derivative, inhibited the enzyme activity of MMP-2, 9, and 14 but not MMP-1, 3 or 7. Daily oral administration of MMI-166 resulted in potent inhibition of metastatic lung...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Maekawa R,Maki H,Wada T,Yoshida H,Nishida-Nishimoto K,Okamoto H,Matsumoto Y,Tsuzuki H,Yoshioka T

    更新日期:2000-01-01 00:00:00

  • Impact of decompressive laminectomy on the functional outcome of patients with metastatic spinal cord compression and neurological impairment.

    abstract::Metastatic spinal cord compression (MSCC) is a frequent phenomenon in advanced tumor diseases with often severe neurological impairments. Affected patients are often treated by decompressive laminectomy. To assess the impact of this procedure on Karnofsky Performance Index (KPI) and Frankel Grade (FG) at discharge, a ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Younsi A,Riemann L,Scherer M,Unterberg A,Zweckberger K

    更新日期:2020-04-01 00:00:00

  • Extent of peritumoral brain edema correlates with prognosis, tumoral growth pattern, HIF1a expression and angiogenic activity in patients with single brain metastases.

    abstract::To analyze the prognostic value of the extent of peritumoral brain edema in patients operated for single brain metastases (BM), we retrospectively evaluated pre-operative magnetic resonance images in a discovery cohort of 129 patients and a validation cohort of 118 patients, who underwent neurosurgical resection of a ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Spanberger T,Berghoff AS,Dinhof C,Ilhan-Mutlu A,Magerle M,Hutterer M,Pichler J,Wöhrer A,Hackl M,Widhalm G,Hainfellner JA,Dieckmann K,Marosi C,Birner P,Prayer D,Preusser M

    更新日期:2013-04-01 00:00:00

  • Growth and metastasis of hypermotile, hyperinvasive cancer cells selected in vitro by rapid locomotion under various conditions.

    abstract::Cancer cells selected from a cultured murine fibrosarcoma by rapid migration through micropore membranes moved considerably faster through such membranes and invaded biological tissues much more efficiently than did the unselected parent cells. The present data show that populations of cells selected by unstimulated m...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Grimstad IA

    更新日期:1988-07-01 00:00:00

  • Bosentan inhibits tumor vascularization and bone metastasis in an immunocompetent skin-fold chamber model of breast carcinoma cell metastasis.

    abstract::Angiogenic factors including endothelin-1 (ET-1) play a key role in the progression of breast metastases to bone. We investigated the impact of ET-1 on the development of bone metastases in an immunocompetent murine skin-fold chamber model. Murine mammary carcinoma 4T1 was injected in a skin-fold chamber implanted on ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Dréau D,Karaa A,Culberson C,Wyan H,McKillop IH,Clemens MG

    更新日期:2006-01-01 00:00:00

  • Syndecan-1: a dynamic regulator of the myeloma microenvironment.

    abstract::Emerging data in myeloma and other cancers indicates that heparan sulfate proteoglycans promote tumor progression by enhancing their growth and metastasis. By acting as key regulators of cell signaling via their interactions with multiple growth and angiogenic factors, heparan sulfates mediate a shift in the microenvi...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Sanderson RD,Yang Y

    更新日期:2008-01-01 00:00:00

  • Bladder metastasis from renal cell carcinoma: retrospective analysis of 65 reported cases.

    abstract::This study was carried out to clarify the presentation, treatment options, and prognosis of renal cell carcinoma (RCC) metastasis to the bladder in which we do not yet have a comprehensive understanding. A systematic Medline, Web of Science, Embase, Google, and Ichushi Web search was performed to identify articles des...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Matsumoto K,Hayakawa N,Nakamura S,Oya M

    更新日期:2015-02-01 00:00:00

  • A novel in vitro assay system for transendothelial tumor cell invasion: significance of E-selectin and alpha 3 integrin in the transendothelial invasion by HT1080 fibrosarcoma cells.

    abstract::The interaction of tumor cells with endothelial cells is a key event in tumor metastasis. We established an in vitro invasion assay system, in which the invasion of tumor cells after interaction with endothelial cells can be examined. Two chamber culture wells separated by porous membrane were used. Human umbilical ve...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Okada T,Okuno H,Mitsui Y

    更新日期:1994-07-01 00:00:00

  • Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis.

    abstract::Metastasis is key to cancer mortality. Understanding its biology is vital for developing strategies to prevent and treat metastasis. Phenotypic assays to either study metastasis or evaluate anti-metastatic drugs are widely used in preclinical research. This technical note discusses the adherence of reporting essential...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Boiy R,Steenbrugge J,Van Deun J,Hendrix A,Meyer E,De Wever O

    更新日期:2018-12-01 00:00:00

  • S100A11 is a potential prognostic marker for clear cell renal cell carcinoma.

    abstract::Clear cell renal cell carcinoma (ccRCC) is one of few cancers with rising incidence in North America. The prognosis of ccRCC is variable and difficult to predict. Stratification of patients according to disease aggressiveness can significantly improve patient management. We investigated the expression of the S100A11 p...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Gabril M,Girgis H,Scorilas A,Rotondo F,Wala S,Bjarnason GA,Ding Q,Evans A,Tawedrous E,Pasic M,Finelli A,Al-Haddad S,Yousef GM

    更新日期:2016-01-01 00:00:00

  • Editorial: Therapy-induced metastasis.

    abstract::The idea for this Special Issue originated from our recent review in Nature Reviews Clinical Oncology entitled "Does the mobilization of circulating tumour cells during cancer therapy cause metastasis?" Martin et al. (Nat Rev Clin Oncol 14:32-44, 2017). While preparing this review, it became evident that an overwhelmi...

    journal_title:Clinical & experimental metastasis

    pub_type: 社论


    authors: Martin OA,Anderson RL

    更新日期:2018-04-01 00:00:00

  • Review of diagnostic, prognostic, and predictive biomarkers in melanoma.

    abstract::Melanoma is an aggressive cutaneous malignancy with rapidly rising incidence. Diagnosis of controversial melanocytic lesions, correct prognostication of patients, selection of appropriate adjuvant and systemic therapies, and prediction of response to a given therapy remain very real challenges. Despite these challenge...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Ankeny JS,Labadie B,Luke J,Hsueh E,Messina J,Zager JS

    更新日期:2018-08-01 00:00:00

  • Carcinoembryonic antigen (CEA) and its receptor hnRNP M are mediators of metastasis and the inflammatory response in the liver.

    abstract::This article discusses the role of carcinoembryonic antigen (CEA) as a facilitator of the inflammatory response and its effect on colorectal cancer hepatic metastasis. Colorectal cancer accounts for 11% of all cancers in the United States and the majority of deaths are associated with liver metastasis. If left untreat...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Thomas P,Forse RA,Bajenova O

    更新日期:2011-12-01 00:00:00

  • Metastasis of mammary tumours in mice: relationship with morphology of primary tumour, and reproductive background of host.

    abstract::The lungs of BR6 breeding female mice which had developed spontaneous mammary tumours, were examined histologically for the presence of metastases. Metastatic nodules, or tumour cell emboli within blood vessels, were found in the lungs of 3 out of 42 (7 per cent) mice with pregnancy-dependent tumours, and in 33 out of...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Lee AE,Pang LS,Rogers LA,Jeffery RE

    更新日期:1983-07-01 00:00:00

  • Inhibition of protein synthesis, pulmonary localization and pulmonary tumour formation by drug-treated tumour cells as a means of predicting their chemosensitivity.

    abstract::Mouse mammary carcinoma cells were exposed in vitro to increasing concentrations of doxorubicin hydrochloride [adriamycin (ADR)] or 5-fluorouracil (5-FU). Uptake of [75Se]selenomethionine (75SeM) in a methionine-deficient medium measured the resulting inhibition of protein synthesis by the tumour cells. This was compa...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Lai T,Stonebridge BR,Black J,Symes MO

    更新日期:1989-07-01 00:00:00

  • Predicting brain metastases for non-small cell lung cancer based on magnetic resonance imaging.

    abstract::In this study the relationship between brain structure and brain metastases (BM) occurrence was analyzed. A model for predicting the time of BM onset in patients with non-small cell lung cancer (NSCLC) was proposed. Twenty patients were used to develop the model, whereas the remaining 69 were used for independent vali...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Yin G,Li C,Chen H,Luo Y,Orlandini LC,Wang P,Lang J

    更新日期:2017-02-01 00:00:00

  • Inhibition of alpha(v)beta3 integrin reduces angiogenesis, bone turnover, and tumor cell proliferation in experimental prostate cancer bone metastases.

    abstract::The growth of metastatic prostate cancer cells in the bone involves an intimate interaction between the tumor cells and various elements of the bone microenvironment, resulting in increased rate of bone turnover and rapid tumor growth. The alpha(v)beta3 integrin has been shown to play an important role in tumor growth...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Nemeth JA,Cher ML,Zhou Z,Mullins C,Bhagat S,Trikha M

    更新日期:2003-01-01 00:00:00

  • Mapping protein signal pathway interaction in sarcoma bone metastasis: linkage between rank, metalloproteinases turnover and growth factor signaling pathways.

    abstract::We applied reverse phase protein microarrays technology to map signal pathway interactions in a discovery set of 34 soft tissue sarcoma (STS) bone metastases compared to healthy bone. Proteins associated with matrix remodeling (MMP), adhesion (FAK Y576/577, Syndecan-1), and growth/survival (IGF1R Y1135/1136, PI3K, EGF...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Conti A,Espina V,Chiechi A,Magagnoli G,Novello C,Pazzaglia L,Quattrini I,Picci P,Liotta LA,Benassi MS

    更新日期:2014-01-01 00:00:00

  • Predictors of prognosis of synchronous brain metastases in small-cell lung cancer patients.

    abstract::Patients with small cell lung cancer (SCLC) are more likely to have synchronous brain metastasis (SBM) at the time of diagnosis than patients with any other extracranial primary malignancy. We sought to identify which factors predicted an increased risk of SBM in SCLC as well as which factors affected the prognosis of...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Reddy SP,Dowell JE,Pan E

    更新日期:2020-08-01 00:00:00

  • Membrane vesicles shed into the extracellular medium by human breast carcinoma cells carry tumor-associated surface antigens.

    abstract::We have compared the pattern of surface antigen expression, as detected by monoclonal antibodies (mAbs), in plasma membranes vs shed membrane vesicles of two human breast carcinoma cell lines, MCF-7 and 8701-BC. Antigen expression was detected on cells by immunofluorescence (IF) analysis, whilst, due to their small di...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Dolo V,Adobati E,Canevari S,Picone MA,Vittorelli ML

    更新日期:1995-07-01 00:00:00

  • miR-200c inhibits invasion and migration in human colon cancer cells SW480/620 by targeting ZEB1.

    abstract::MicroRNAs are a class of ≈22-nt noncoding single-strand RNAs regulating gene expression postscriptionally. Metastasis caused poor prognosis in colorectal cancer patients and half of the patients developed metastatic lesions when admission. Here we investigated the possible roles of microRNAs in regulating metastasis i...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Chen ML,Liang LS,Wang XK

    更新日期:2012-06-01 00:00:00

  • High-dose ionizing radiation-induced hematotoxicity and metastasis in mice model.

    abstract::Radiotherapy induces untargeted effects on normal tissues such as bone marrow. So alteration of microenvironment by ionizing irradiation is supposed to influence dynamic host-cancer ecosystem affecting cancer behavior including metastasis. Herein, the incidence of lung metastasis after high-dose irradiation has been i...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Shin JW,Son JY,Raghavendran HR,Chung WK,Kim HG,Park HJ,Jang SS,Son CG

    更新日期:2011-12-01 00:00:00

  • Gene transduction of NK4, HGF antagonist, inhibits in vitro invasion and in vivo growth of human pancreatic cancer.

    abstract::In this study, we investigated the therapeutic effects of adenovirally-mediated transfer of the sequence of NK4, an antagonist for hepatocyte growth factor (HGF), against human pancreatic carcinoma. HGF has been implicated to play an important role in invasion and metastasis of various human cancers through tumor-stro...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Maehara N,Nagai E,Mizumoto K,Sato N,Matsumoto K,Nakamura T,Narumi K,Nukiwa T,Tanaka M

    更新日期:2002-01-01 00:00:00

  • Is adjuvant treatment with vinblastine effective in reducing the occurrence of distant metastasis in limited squamous cell lung cancer? A randomized study.

    abstract::In order to study the usefulness of treatment with vinblastine (VLB) in the prevention of cancer metastasis in squamous cell lung cancer, 50 patients with locoregional disease were randomized to receive either locoregional RT alone (group A) or a weekly intravenous bolus injection of VLB (6 mg/m2) concurrently with an...

    journal_title:Clinical & experimental metastasis

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Schallier DC,De Neve WJ,De Greve JL,Van Belle SP,De Wasch GJ,Dotremont G,Storme GA

    更新日期:1988-01-01 00:00:00

  • Circulating microRNAs in head and neck cancer: a scoping review of methods.

    abstract::Circulating microRNAs have been described as head and neck cancer biomarkers in multiple anatomical subsites including the oral cavity, nasopharynx, larynx, salivary glands and the skin. While there is an expanding volume of published literature showing the significance of individual or panels of microRNAs, the clinic...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Dharmawardana N,Ooi EH,Woods C,Hussey D

    更新日期:2019-06-01 00:00:00

  • Adhesion properties, intermediate filaments and malignant behaviour of head and neck squamous cell carcinoma cells in vitro.

    abstract::This study compared phenotype and behaviour of seven head and neck squamous cell carcinoma (HNSCC) cell lines and normal epithelial cells. Indications were found that in HNSCC cells: 1) loss of the cell-cell adhesion molecule E-cadherin was correlated with loss of epithelioid cell morphology but not with loss of cell-...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Tomson AM,Scholma J,Meijer B,Koning JG,de Jong KM,van der Werf M

    更新日期:1996-11-01 00:00:00

  • Functional blocking of specific integrins inhibit colonic cancer migration.

    abstract::For more effective oncological management of disseminated colorectal cancer, therapies must be devised that target the different individual stages of metastasis development. Recent work showed that integrin subunits alpha2, alpha6 and beta4 are involved in the colorectal cancer cell extravasation process. By means of ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Robertson JH,Yang SY,Winslet MC,Seifalian AM

    更新日期:2009-01-01 00:00:00

  • Phenotypic and functional characteristics of tumour-derived microvascular endothelial cells.

    abstract::We recently developed a method for the isolation and purification of tumour-derived endothelium. In this study the phenotypic and functional properties of human tumour-derived microvascular endothelial cells (TdMEC) were examined. Endothelium obtained from human adrenal gland specimens (HAMEC) was used as a reference ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Alessandri G,Chirivi RG,Fiorentini S,Dossi R,Bonardelli S,Giulini SM,Zanetta G,Landoni F,Graziotti PP,Turano A,Caruso A,Zardi L,Giavazzi R,Bani MR

    更新日期:1999-01-01 00:00:00

  • Multiple effects of transfection with interleukin 2 and/or interferon gamma on the behavior of mouse T lymphoma cells.

    abstract::We have previously reported that transfection of mouse interferon gamma (IFN-gamma) in H-2Kk-positive BW variants (BW-Sp3) abolishes tumorigenicity and reduces metastatic capacity. To further increase the immunogenicity of BW-Sp3 cells, the gene for human interleukin 2 (huIL-2) was transfected in these cancer cells. S...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Geldhof AB,Van Ginderachter J,Vandersteen P,Raes G,De Baetselier P

    更新日期:1998-07-01 00:00:00

  • Overview and update of the phase III Multicenter Selective Lymphadenectomy Trials (MSLT-I and MSLT-II) in melanoma.

    abstract::This short review offers an update on the first and second Multicenter Selective Lymphadenectomy Trials (MSLT-I and MSLT-II) for patients with melanoma, and briefly traces the development of intraoperative lymphatic mapping and sentinel node biopsy. ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,多中心研究,评审


    authors: Morton DL

    更新日期:2012-10-01 00:00:00