Abstract：
:Pancreatic cancer demonstrates a strong resistance to anticancer drugs, presumably due to its resistance to drug induced apoptosis. Although gemcitabine (GEM) might be partially effective for treating advanced pancreatic cancer, its efficacy is still less than satisfactory. Galectin-3 (gal-3), a member of the β-galactoside-binding protein family, is a multifunctional protein with roles in tumor cell adhesion, proliferation, differentiation, angiogenesis, metastasis, and apoptosis. We have utilized gal-3 small interfering RNA (siRNA) to probe whether gal-3 regulates anticancer drug-induced apoptosis in pancreatic cancer cells. We found that Gal-3 siRNA augmented GEM- and cisplatin-induced apoptosis in pancreatic cancer cell lines in vitro. Mitochondrial depolarization induction was increased in gal-3-silenced cells after GEM treatment, resulting in activation of caspase-9, but not caspase-8. Akt phosphorylation was significantly downregulated in gal-3- silenced cells in association with apoptosis. Moreover, intratumoral administration of gal-3 siRNA increased the GEM sensitivity of tumor xenografts produced by subcutaneous inoculation of pancreatic cancer cells into nude mice. These results suggest that gal-3 might provide a novel therapeutic target in pancreatic cancer.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Kobayashi T,Shimura T,Yajima T,Kubo N,Araki K,Wada W,Tsutsumi S,Suzuki H,Kuwano H,Raz Adoi
10.1007/s10585-011-9376-xsubject
Has Abstractpub_date
2011-04-01 00:00:00pages
367-76issue
4eissn
0262-0898issn
1573-7276journal_volume
28pub_type
杂志文章abstract：:We previously isolated thymosin beta15 from highly metastatic Dunning rat prostatic carcinoma cells. Immunohistochemical study of human prostate cancer specimens revealed a general correlation between Gleason grade and thymosin beta15 expression, with high-grade (more malignant) tumors showing increased staining compa...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006540824969
更新日期：1998-04-01 00:00:00
abstract：:Extrathyroidal extension (ETE) of papillary thyroid carcinoma (PTC) is common and clinical presentation can vary from minimal to extensive locoregional involvement. Although PTC is generally considered the most benign among all thyroid carcinomas, it may present with local invasion with poor prognosis. Our retrospecti...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9800-3
更新日期：2016-08-01 00:00:00
abstract：:A previous study from our laboratory suggested that prostate cancer metastasis to bone may be mediated, in part, by preferential adhesion to human bone marrow endothelial (HBME) cells. Tumor cell adhesion to endothelial cells may be modulated by the effect of cytokines on cell adhesion molecules (CAMs). Tumor necrosis...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1013849123736
更新日期：2002-01-01 00:00:00
abstract：:The interaction between circulating tumor cells and blood components, mainly platelets, plays an important role during metastasis. In this study, we prepared liposomes containing the platelet aggregation inhibitor Cilostazol (Cil-L). The objective of this study was to investigate the effect of this Cil-L on platelet a...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-009-9299-y
更新日期：2010-01-01 00:00:00
abstract：:This article discusses the role of carcinoembryonic antigen (CEA) as a facilitator of the inflammatory response and its effect on colorectal cancer hepatic metastasis. Colorectal cancer accounts for 11% of all cancers in the United States and the majority of deaths are associated with liver metastasis. If left untreat...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-011-9419-3
更新日期：2011-12-01 00:00:00
abstract：:Vascularization of solid tumors is thought to occur by sprouting or intussusceptive angiogenesis, co-option of existing vessels, and vasculogenic mimicry after the onset of tumor hypoxia, when the tumor radius exceeds the oxygen diffusion distance. In contrast, here we show that individual endothelial cells that are i...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-009-9257-8
更新日期：2009-01-01 00:00:00
abstract：:The present study assessed the effects of dietary fat on spontaneous metastasis of Lewis lung carcinoma in mice. Three-week old male C57BL/6 mice were fed the AIN-93G diet or a 45% fat diet (% kcal.) for 7 weeks before they were subcutaneously injected with 2.5 × 10⁵ viable carcinoma cells. The primary tumor was resec...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-010-9347-7
更新日期：2010-12-01 00:00:00
abstract：:We have used 5'-deoxy-5'-S isobutyl-thioadenosine (SIBA), an analog of S-adenosylhomocysteine, alone or in association with a methionine-depleted diet in order to obtain an antitumoral effect in two different tumor models: a transplantable rat rhabdomyosarcoma (RMS-J1) induced by i.m. injection of nickel and the well-...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01580402
更新日期：1988-01-01 00:00:00
abstract：:Cancer cells with the surface marker profile CD44+/CD24- have previously been described to possess cancer stem cell-like properties. This manuscript evaluates those properties in ovarian cancer cell lines. The proportion of CD44+/CD24- cells corresponded to the clinical aggressiveness of each ovarian cancer cell line ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9482-4
更新日期：2012-12-01 00:00:00
abstract：:Cancer stem cells drive the metastatic cascade by undergoing epithelial to mesenchymal transition (EMT) and again mesenchymal to epithelial transition (MET). Using multiple breast cancer cell lines including cigarette smoke induced breast cancer cells and tumor derived primary cells from patient sample; we developed a...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9809-7
更新日期：2016-10-01 00:00:00
abstract：:Use of heparin derivatives in several cancer types revealed that anticoagulant therapies have a beneficiary side effect: delay of tumor progression. Since there are no data on human melanoma, we have analyzed the effect of heparins in preclinical models. Neither unfractionated heparin (UFH), nor its low molecular weig...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-005-3859-6
更新日期：2005-01-01 00:00:00
abstract：:Human CD44 standard isoform (hCD44s) cDNA regulated by a high-expressing promoter was transfected into Balb/c 3T3 cells and the tumorigenic and metastatic capacities of the transfectants were investigated in nude mice at the subcutaneous site. One of three transfectants was tumorigenic. hCD44s expression was lost in t...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006568103588
更新日期：1998-01-01 00:00:00
abstract：:Metastatic variant sublines of the murine RAW117 large cell lymphoma or lymphosarcoma have been established in vitro by sequential cycles of harvesting of liver tumor nodules after intravenous inoculation of tumor cell suspensions into syngeneic BALB/c mice. After five and ten in vivo selections for liver colonization...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00132928
更新日期：1984-07-01 00:00:00
abstract：:Repeated administration of the hepatic lectin blocking agents D-galactose or arabinogalactan completely prevented the settling of metastatic cells of sarcoma L-1 tumor in the liver of Balb/c mice and greatly reduced the colonization process of highly metastatic ESb lymphoma cells of the liver of DBA/2 mice. Therefore,...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01784842
更新日期：1988-03-01 00:00:00
abstract：:The BRMS1 metastasis suppressor was recently shown to negatively regulate NF-kappaB signaling and down regulate NF-kappaB-dependent uPA expression. Here we confirm that BRMS1 expression correlates with reduced NF-kappaB DNA binding activity in independently derived human melanoma C8161.9 cells stably expressing BRMS1....
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-009-9235-1
更新日期：2009-01-01 00:00:00
abstract：:Insulin-like growth factor I receptor (IGF-IR) is critical to cell survival and growth and altered IGF-IR expression is found in many human cancers. However, its expression and potential role in gastric cancer development and progression has not been explored. The IGF-IR expression level was determined via immunohisto...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-005-1198-2
更新日期：2004-01-01 00:00:00
abstract：:Tumor metastasis often confers poor prognosis for cancer patients due to lack of comprehensive strategy in dealing with cells growing in different environment. Current anticancer therapies have incomplete effectiveness because they were designed assuming metastatic tumors behave similarly in different organs. We hypot...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-015-9761-y
更新日期：2016-02-01 00:00:00
abstract：:Despite an initial response to antihormonal therapies, the development of resistance will occur in a significant number of breast cancer patients. The mechanisms that underlie acquired resistance are not yet clear. Using a previously established in vitro cell model of tamoxifen resistance in MCF7 cells, shown to displ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/b:clin.0000037697.76011.1d
更新日期：2004-01-01 00:00:00
abstract：:About 30-40% of patients with renal cell carcinoma (RCC) will develop metastasis after curative nephrectomy. There is a strong need to identify the early metastasis with conventional and molecular risk factors. The present study aimed to test if analysis of the CA9 gene can provide useful information to predict early ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9064-z
更新日期：2007-01-01 00:00:00
abstract：:We have previously reported that induction of MMP-2 activation by Concanavalin A (ConA) in MDA-MB-231 human breast cancer cells involves both transcriptional and post-transcriptional mechanisms, and that the continuous presence of ConA is required for MMP-2 activation (Yu et al. Cancer Res, 55, 3272-7, 1995). In an ef...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006580406314
更新日期：1998-02-01 00:00:00
abstract：:Activation of nuclear factor-κB (NF-κB) has been implicated in metastasis of pancreatic cancer. We investigated the effects of the novel NF-κB inhibitor dehydroxymethylepoxyquinomicin (DHMEQ) on the inhibition of liver metastasis of pancreatic cancer in a mouse model of clinical liver metastasis. Nude mice were xenogr...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9544-7
更新日期：2013-04-01 00:00:00
abstract：:Very few tissues in vivo are resistant to invasion by malignant cells. In this paper, confrontation cultures consisting of chick embryonic notochord fragments and cell aggregates from three malignant neurogenic cell lines showed a resistance of the embryonic organ to malignant invasion by tumor cells. The mechanism of...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01769359
更新日期：1991-07-01 00:00:00
abstract：:Metastasis is the most devastating aspect of cancer and it is the main cause of morbidity and mortality in cancer patients. Tumor cell adhesion to the vascular endothelial cell lining is an important step in metastatic progression and is prompted by platelets. Mucin 1 is over-expressed and aberrantly glycosylated in m...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9461-9
更新日期：2012-06-01 00:00:00
abstract：:Circulating microRNAs have been described as head and neck cancer biomarkers in multiple anatomical subsites including the oral cavity, nasopharynx, larynx, salivary glands and the skin. While there is an expanding volume of published literature showing the significance of individual or panels of microRNAs, the clinic...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-019-09961-6
更新日期：2019-06-01 00:00:00
abstract：:Treating metastatic osteosarcoma (OS) remains a challenge in oncology. Current treatment strategies target the primary tumour rather than metastases and have a limited efficacy in the treatment of metastatic disease. Metastatic cells have specific features that render them less sensitive to therapy and targeting these...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-011-9384-x
更新日期：2011-06-01 00:00:00
abstract：:The spinal column is the most frequent site of bone metastasis in patients with breast cancer. It is important to understand how the pattern of vertebral lesions may be affected by the introduction of modern cancer therapies. The purpose of this study was to characterize changes in the radiological appearance of spina...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-008-9219-6
更新日期：2009-01-01 00:00:00
abstract：:Development of central nervous system (CNS) metastases in breast cancer (BC) is associated with poor prognosis. The incidence of CNS metastases in metastatic BC is reported to be about 10-16 %, but different subtypes of BC are associated with different risk of developing CNS metastases. We retrospectively analysed the...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9594-5
更新日期：2013-12-01 00:00:00
abstract：:Accumulating evidences indicate that long non-coding RNAs (lncRNAs) play important roles in several biological processes and dysregulated lncRNAs are involved in different kinds of cancer and are associated with carcinogenesis, metastasis, and prognosis of cancer. The role of a new lncRNA LOC100130476 in gastric cardi...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-016-9794-x
更新日期：2016-06-01 00:00:00
abstract：:Recent studies have indicated that KHT fibrosarcoma or B16 melanoma cell variants capable of forming experimental metastases in the lungs of mice after i.v. injection are created stochastically at high rates (approximately 10(-5)/cell/generation). Expression of this phenotype is unstable and hence expanding population...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00117930
更新日期：1986-07-01 00:00:00
abstract：:Single cell progenies (SCPs) inherit biological properties from their isogenetic mother cells. If a single cancer cell can give rise to progenies, which can be passaged sustainably in vitro and produce tumor in xenotransplantation, the cell should be cancer initiating cell. CD133 (Prominin-1, Prom1) is the marker of h...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-010-9341-0
更新日期：2010-10-01 00:00:00
