Abstract:
:The effects of treatment of human lung carcinoma cell line A 549 with recombinant DNA-derived human leukocyte interferons A (rIFN-alpha A) or D (rIFN-alpha D), and human lymphoblastoid interferon (Wellferon) on in vitro cell invasion were investigated in a quantitative invasion assay using human amnion. The A 549 cells treated with IFN for one day were incubated on the denuded basement membrane of the amnion in the absence of IFN, and cells which penetrated the full thickness of the connective tissue barrier were measured after 4 days. A dose-dependent inhibition of cell invasion was produced by the recombinant IFNs. The one day treatment of cells with 2.4 X 10(3) to 1.8 X 10(4) units/ml of rIFN-alpha A resulted in a 60-80 per cent inhibition of invasiveness compared to untreated cells. After a one day exposure of cells to 2.2 X 10(4) units/ml of rIFN-alpha D, cell invasion was reduced by approximately 70 per cent; a concentration of 4.4 X 10(3) units/ml had no apparent effect. Similar treatment with lymphoblastoid IFN (6 X 10(4) units/ml) had no significant effect on cell invasion. Accompanying the one day exposure to rIFN-alpha A (1.8 X 10(4) units/ml) or rIFN-alpha D (2.2 X 10(4) units/ml), (2',5') oligo (A) synthetase activity was induced approximately 20-fold; a 4-fold induction of enzyme activity was found in cells exposed to lymphoblastoid IFN (6 X 10(4) units/ml). After exposure of A 549 cells to the three IFNs at these concentrations, no significant alteration of the ability of the cells to attach to the basement membrane was found. Moreover, none of the one day IFN treatment regimens were cytocidal, and cell proliferation ability was not affected. This model system may be useful for investigating anti-invasive activity of other IFN types and subtypes.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Ravine TJ,Ledinko Ndoi
10.1007/BF00117932subject
Has Abstractpub_date
1986-07-01 00:00:00pages
191-203issue
3eissn
0262-0898issn
1573-7276journal_volume
4pub_type
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journal_title:Clinical & experimental metastasis
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pub_type: 临床试验,杂志文章,随机对照试验
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pub_type: 杂志文章,评审
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