Abstract:
:Metastasis, responsible for most deaths from breast cancer (BC), is a multistep process leading to cancer cell spread. Extracellular matrix (ECM)-related adhesion and apoptosis resistance play pivotal role in metastasis. Ras suppressor-1 (RSU-1) localizes to cell-ECM adhesions and binds to pro-survival adhesion protein PINCH-1. Little is known about the role of RSU-1 in BC. In the present study, we investigated the role of RSU-1 in BC metastasis using two BC cell lines that differ in terms of their metastatic potential and a set of 32 human BC samples from patients with or without lymph node metastasis. We show that RSU-1 is upregulated in the aggressive MDA-MB-231 cells compared to MCF-7 and that its silencing by siRNA leads to upregulation of PINCH-1, induction of proliferation and reduction of apoptosis through downregulation of the pro-apoptotic gene p53-upregulated-modulator-of-apoptosis (PUMA). Our findings in the cell lines were further validated in the human BC tissues where normal adjacent tissues were used as controls. We demonstrate for the first time, that RSU-1 expression is upregulated in metastatic BC samples and downregulated in non-metastatic while it is negatively correlated with PINCH-1 and positively correlated with PUMA expression, suggesting that a pro-apoptotic mechanism is in place in metastatic BC samples and identifying RSU-1 as a potentially interesting molecule that needs to be evaluated further as a novel BC metastasis biomarker.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Giotopoulou N,Valiakou V,Papanikolaou V,Dubos S,Athanassiou E,Tsezou A,Zacharia LC,Gkretsi Vdoi
10.1007/s10585-015-9701-xsubject
Has Abstractpub_date
2015-03-01 00:00:00pages
255-65issue
3eissn
0262-0898issn
1573-7276journal_volume
32pub_type
杂志文章abstract::Blood samples, bone marrow, tumours and metastases where possible were collected from SCID mice bearing orthotopic xenografts of the triple-negative MDA-MB-468 cell line or a transplantable ER-positive patient derived xenograft (ED-03), and assessed using human-specific, tandem-nested RT-qPCR for markers relating to d...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-019-09977-y
更新日期:2019-08-01 00:00:00
abstract::Little is known about the biological characteristics that determine the prognosis of colorectal cancer (CRC) liver metastases. In previous work we reported three different histological patterns of the tumour-liver interface of CRC liver metastases, termed the pushing, replacement and desmoplastic growth pattern (GP). ...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-012-9469-1
更新日期:2012-08-01 00:00:00
abstract::Tumor metastasis often confers poor prognosis for cancer patients due to lack of comprehensive strategy in dealing with cells growing in different environment. Current anticancer therapies have incomplete effectiveness because they were designed assuming metastatic tumors behave similarly in different organs. We hypot...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-015-9761-y
更新日期:2016-02-01 00:00:00
abstract::Breast cancer metastasis to the brain develops after a clinical latency of years to even decades, suggesting that colonization of the brain is the most challenging step of the metastatic cascade. However, the underlying mechanisms used by breast cancer cells to successfully colonize the brain's microenvironment remain...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-017-9839-9
更新日期:2017-02-01 00:00:00
abstract::ECA109 human oesophageal carcinoma cells were injected either subcutaneously or intraperitoneally into BALB/CATc 1-nu/nu mice. After 23 weeks tumours were examined histologically and by scanning electron microscopy. Subcutaneous ECA109 tumours were well-delineated without signs of invasion. By contrast, intra-abdomina...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF00132927
更新日期:1984-07-01 00:00:00
abstract::Metastatic variant sublines of the murine RAW117 large cell lymphoma or lymphosarcoma have been established in vitro by sequential cycles of harvesting of liver tumor nodules after intravenous inoculation of tumor cell suspensions into syngeneic BALB/c mice. After five and ten in vivo selections for liver colonization...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF00132928
更新日期:1984-07-01 00:00:00
abstract::Numerous cancer patients fail standard chemotherapy or develop resistance to chemotherapy during the course of treatment. The purpose of this study is to elucidate the overall response of cells obtained from cancer patients and from normal individuals to chemotherapeutic agents. We analysed the chemosensitivity of can...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-006-9034-x
更新日期:2006-01-01 00:00:00
abstract::We have examined the role of platelet-endothelial cell adhesion molecule-1 (PECAM-1/CD31) during the transendothelial migration of melanoma cells using a novel in vitro system. Comparable studies have suggested the involvement of PECAM-1 in leukocyte transendothelial migration. Such studies have been confirmed using i...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1011884807746
更新日期:2000-01-01 00:00:00
abstract::Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy with poor prognosis, particularly for patients with metastatic disease. Treatment for oligometastatic presentation has been reported in recent literature, but the role of intraperitoneal chemotherapy for patients with peritoneal metastases (PM) remain...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章,评审
doi:10.1007/s10585-021-10074-2
更新日期:2021-01-24 00:00:00
abstract::Mouse mammary carcinoma cells were exposed in vitro to increasing concentrations of doxorubicin hydrochloride [adriamycin (ADR)] or 5-fluorouracil (5-FU). Uptake of [75Se]selenomethionine (75SeM) in a methionine-deficient medium measured the resulting inhibition of protein synthesis by the tumour cells. This was compa...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF01753663
更新日期:1989-07-01 00:00:00
abstract::Intravenous infusions of perfluorochemical emulsions, combined with administration of inspired oxygen or carbogen have been found to improve tumor oxygenation and increase the response of solid tumors in animals to radiotherapy. Fluosol-DA 20 per cent, the only perfluorochemical emulsion currently approved for testing...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF00053472
更新日期:1986-01-01 00:00:00
abstract::Metastatic spinal cord compression (MSCC) is a frequent phenomenon in advanced tumor diseases with often severe neurological impairments. Affected patients are often treated by decompressive laminectomy. To assess the impact of this procedure on Karnofsky Performance Index (KPI) and Frankel Grade (FG) at discharge, a ...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-019-10016-z
更新日期:2020-04-01 00:00:00
abstract::Tumor metastases are extremely rare in striated muscles. This is surprising given the fact that this tissue constitutes 60% of body weight. The present study focuses on small molecules produced and secreted by muscle cells which possess anti-cancer activity in vivo. Recently we have shown that a low molecular weight f...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1006649617918
更新日期:1999-01-01 00:00:00
abstract::In breast tumors, high levels of leptin have been associated with increased incidence of breast cancer metastasis. Breast cancer metastasis is directly associated with breast cancer cell invasion. However, whether leptin could augment breast cancer cell invasion is not known. Here we showed that leptin increased the i...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-008-9231-x
更新日期:2009-01-01 00:00:00
abstract::Lymphangiogenesis plays a crucial role in promoting cancer metastasis to sentinel lymph nodes (LNs) and beyond. Increasing data have shown that simvastatin, a cholesterol-lowering medication for the prevention of cardiovascular diseases, is involved in tumor growth and dissemination, and endothelial functions. This st...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-018-9940-8
更新日期:2018-12-01 00:00:00
abstract::Electrochemotherapy (ECT) is a novel treatment for recurrent or in-transit unresectable melanoma metastases based on the administration of anti-neoplastic drugs followed by cancer cell electroporation. Whether ECT can also induce anti-tumour immunity is unclear. We addressed this issue investigating the presence of de...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-012-9505-1
更新日期:2013-01-01 00:00:00
abstract::Human fibrosarcoma HT1080 cell surface phenotype analysis revealed the expression of "cluster of differentiation 15" (CD15) antigen and to a lesser extent, of "very late antigen-4" (VLA-4). Expression of "endothelial-leukocyte adhesion molecule-1" (ELAM-1) was negligible on resting human umbilical vascular endothelial...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1018487213157
更新日期:1997-11-01 00:00:00
abstract::The chemokine CXCL12/SDF-1 and its receptors CXCR4 and CXCR7 play a major role in tumor invasion, proliferation and metastasis. Since both receptors are overexpressed on distinct tumor cells and on the tumor vasculature, we evaluated their potential as targets for detection of cancers by molecular imaging. We synthesi...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-011-9403-y
更新日期:2011-12-01 00:00:00
abstract::Metastasis is a vital target for cancer treatment, since the majority of cancer patients die from metastatic, rather than the primary disease. KiSS1 has been identified as a metastasis suppressor gene in melanoma and breast carcinomas. We show here that KiSS1 is also a metastasis suppressor in human ovarian cancer. Ov...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-005-8186-4
更新日期:2005-01-01 00:00:00
abstract::We have previously reported that transfection of mouse interferon gamma (IFN-gamma) in H-2Kk-positive BW variants (BW-Sp3) abolishes tumorigenicity and reduces metastatic capacity. To further increase the immunogenicity of BW-Sp3 cells, the gene for human interleukin 2 (huIL-2) was transfected in these cancer cells. S...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1006585525399
更新日期:1998-07-01 00:00:00
abstract::Literature data show that butyric acid derivatives bear a dose-dependent differentiative anti-proliferative activity on cancer cell lines and that apoptosis induction may play a major role. Although it was recently shown that solid lipid nanospheres (SLNs) are a suitable tool for several in vivo drug administration ro...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1013186331662
更新日期:2000-01-01 00:00:00
abstract::Angiogenic cytokines in the plasma and serum of cancer patients may serve as 'surrogate' markers of tumour neoangiogenesis. Serum VEGF correlates with disease stage in colorectal cancer (CRC), but the role of bFGF in CRC is uncertain. This study aimed to assess plasma bFGF levels in CRC patients before treatment, duri...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1021322201816
更新日期:2002-01-01 00:00:00
abstract::Radiation therapy is an effective means of achieving local control in a wide range of primary tumours, with the reduction in the size of the tumour(s) thought to mediate the observed reductions in metastatic spread in clinical trials. However, there is evidence to suggest that the complex changes induced by radiation ...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章,评审
doi:10.1007/s10585-017-9867-5
更新日期:2018-04-01 00:00:00
abstract::We have previously isolated a series of MCF-7 human breast cancer cell variants which no longer require estrogen-supplementation for tumor growth in nude mice (Clarke et al. Proc Natl Acad Sci USA 86: 3649-3653, 1989). We now report that these hormone-independent and hormone-responsive variants (MIII, MCF7/LCC1) can i...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF00880062
更新日期:1993-01-01 00:00:00
abstract::In this study the relationship between brain structure and brain metastases (BM) occurrence was analyzed. A model for predicting the time of BM onset in patients with non-small cell lung cancer (NSCLC) was proposed. Twenty patients were used to develop the model, whereas the remaining 69 were used for independent vali...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-016-9833-7
更新日期:2017-02-01 00:00:00
abstract::The colon carcinoma cell line HT-29 was used to explore the potential of interleukin-4 (IL-4) and tumor necrosis factor alpha (TNF-alpha) to modify integrin expression and adhesive functions of tumor cells in vitro and to examine corresponding metastatic effects in vivo. Preincubation of HT-29 cells with 100 U/ml of I...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF00121213
更新日期:1996-03-01 00:00:00
abstract::Cancer cells with the surface marker profile CD44+/CD24- have previously been described to possess cancer stem cell-like properties. This manuscript evaluates those properties in ovarian cancer cell lines. The proportion of CD44+/CD24- cells corresponded to the clinical aggressiveness of each ovarian cancer cell line ...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-012-9482-4
更新日期:2012-12-01 00:00:00
abstract::Cancer cells selected from a cultured murine fibrosarcoma by rapid migration through micropore membranes moved considerably faster through such membranes and invaded biological tissues much more efficiently than did the unselected parent cells. The present data show that populations of cells selected by unstimulated m...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF01753573
更新日期:1988-07-01 00:00:00
abstract::We examined the in vivo anti-tumor activity of the benzoic acid derivative, TAC-101 (4-[3,5-bis(trimethylsilyl)benzamido] benzoic acid), for intrahepatic spread of JHH-7 human hepatocellular carcinoma (HCC) cells and its mechanism of action. Oral administration of TAC-101 markedly inhibited liver tumor of JHH-7 cells ...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1006567229929
更新日期:1998-10-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide with increasing incidence. Despite curative surgical resection and advanced chemotherapy, its survival rate remains low. The presence of microvascular invasion and occult metastasis is one of the major causes for this poor outcome. MDM2 Binding...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-015-9706-5
更新日期:2015-04-01 00:00:00