Abstract：
:Electrochemotherapy (ECT) is a novel treatment for recurrent or in-transit unresectable melanoma metastases based on the administration of anti-neoplastic drugs followed by cancer cell electroporation. Whether ECT can also induce anti-tumour immunity is unclear. We addressed this issue investigating the presence of dendritic cells (DCs) in the inflammatory infiltrate of ECT-treated lesions. Biopsies from melanoma patients (n = 9) were taken before ECT (T0), at d7 and d14 after treatment and studied by immunofluorescence with DCs-related antibodies. Epidermal Langerin(+) Langerhans cells (LCs) were the most represented subset before treatment. ECT induced a significant reduction in epidermal LCs number at d7 (p < 0.001), while they were completely replaced at d14. Similarly, the few LCs observed intermingled with metastatic melanoma cells at T0 decreased after treatment (p < 0.001), suggesting an ECT-induced activation of LCs. Consistently, at d1 after ECT (n = 3 patients), LCs were found to express CCR7, which mediates LCs migration to regional lymph nodes, and CD83, the typical DCs maturation marker. In contrast, plasmacytoid DCs (pDCs) were not present at T0, but significantly increased after ECT both in melanoma metastasis (p < 0.001) and perilesionally (p < 0.05). Similarly, CD1c(+) dermal DCs (dDCs), observed in low number before ECT, strongly increased at d7 and even more at d14 (p < 0.05 and p < 0.001, respectively). Notably, some dDCs expressed CD83. These data suggest that ECT promotes LCs migration from the tumour to draining lymph nodes and pDCs and dDCs recruitment at the site of the lesion. These findings may help to design new strategies of in situ DCs vaccination in cancer patients.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Gerlini G,Sestini S,Di Gennaro P,Urso C,Pimpinelli N,Borgognoni Ldoi
10.1007/s10585-012-9505-1subject
Has Abstractpub_date
2013-01-01 00:00:00pages
37-45issue
1eissn
0262-0898issn
1573-7276journal_volume
30pub_type
杂志文章abstract：:In this study, we investigated the therapeutic effects of adenovirally-mediated transfer of the sequence of NK4, an antagonist for hepatocyte growth factor (HGF), against human pancreatic carcinoma. HGF has been implicated to play an important role in invasion and metastasis of various human cancers through tumor-stro...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1016395316362
更新日期：2002-01-01 00:00:00
abstract：:Considering recent findings that cyclooxygensase-2 (COX-2) is involved in the progression of colorectal carcinoma (CRC), the role of COX-2 in promoting invasion and angiogenesis was investigated by evaluating the relationship of COX-2 expression to various clinicopathological variables, including plasminogen activatin...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1020392309715
更新日期：2002-01-01 00:00:00
abstract：:Inflammatory breast cancer (IBC) is the most lethal form of locally advanced breast cancer known. IBC carries a guarded prognosis primarily due to rapid onset of disease, typically within six months, and the propensity of tumor emboli to invade the dermal lymphatics and spread systemically. Although the clinical manif...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1015518114931
更新日期：2002-01-01 00:00:00
abstract：:Literature data show that butyric acid derivatives bear a dose-dependent differentiative anti-proliferative activity on cancer cell lines and that apoptosis induction may play a major role. Although it was recently shown that solid lipid nanospheres (SLNs) are a suitable tool for several in vivo drug administration ro...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1013186331662
更新日期：2000-01-01 00:00:00
abstract：:MUC1 is a transmembrane glycoprotein expressed by normal breast epithelium and virtually all breast cancers. MUC1 is normally restricted to the apical surface of epithelia and loss of this polarized distribution in breast carcinomas is associated with lymph node metastasis. Our previous work found that MUC1 can bind i...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-005-3098-x
更新日期：2005-01-01 00:00:00
abstract：:A variant cell line (EL-4ad) which adhered to a tissue culture dish was isolated from highly metastatic EL-4 murine T-lymphoma. The experimental and spontaneous metastatic ability of EL-4ad was lower than that of the EL-4 parent cell line. The cell surface phenotypes of both cell lines were CD2+3+4-8-45+TCR alpha beta...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00058169
更新日期：1992-09-01 00:00:00
abstract：:Primary tumor growth and metastasis depend on angiogenesis, which is determined by the balance between proangiogenic and antiangiogenic molecules. Interferon (IFN)-alpha and -beta inhibit angiogenesis through downregulation of interleukin-8, matrix metalloproteinase-9, and basic fibroblast growth factor. To provide ev...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1020923326441
更新日期：2002-01-01 00:00:00
abstract：:The mechanisms that control the insidiously invasive nature of malignant gliomas are poorly understood, and their study would be facilitated by an in vivo model that is easy to manipulate and inexpensive. The developing chick embryo brain was assessed as a new xenograft model for the production, growth, and study of h...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-005-7889-x
更新日期：2005-01-01 00:00:00
abstract：:The purpose of this study was to determine the subpopulation dynamics of human colon carcinoma (HCC) cells growing at orthotopic (cecum, liver) or ectopic (subcutis, kidney, spleen) sites in nude mice and to correlate any outgrowth of distinct clones with the differential expression of metastasis-related genes. Low me...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1018400826845
更新日期：1997-03-01 00:00:00
abstract：:Metastasis is key to cancer mortality. Understanding its biology is vital for developing strategies to prevent and treat metastasis. Phenotypic assays to either study metastasis or evaluate anti-metastatic drugs are widely used in preclinical research. This technical note discusses the adherence of reporting essential...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-018-9944-4
更新日期：2018-12-01 00:00:00
abstract：:Radiation therapy is an effective means of achieving local control in a wide range of primary tumours, with the reduction in the size of the tumour(s) thought to mediate the observed reductions in metastatic spread in clinical trials. However, there is evidence to suggest that the complex changes induced by radiation ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-017-9867-5
更新日期：2018-04-01 00:00:00
abstract：PURPOSE:To evaluate lymphangiogenesis in patients with breast carcinoma, explore the underlying mechanism, and study the relationship between lymphangiogenesis and progression of breast carcinoma. METHODS:Sixty-one cases of breast carcinoma with complete clinical and pathological data were analyzed. Using an anti-podo...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-008-9180-4
更新日期：2008-01-01 00:00:00
abstract：:The chemokine CXCL12/SDF-1 and its receptors CXCR4 and CXCR7 play a major role in tumor invasion, proliferation and metastasis. Since both receptors are overexpressed on distinct tumor cells and on the tumor vasculature, we evaluated their potential as targets for detection of cancers by molecular imaging. We synthesi...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-011-9403-y
更新日期：2011-12-01 00:00:00
abstract：:To provide an investigative tool for the study of osteosarcoma (OSA) biology we have developed a syngeneic (balb/c) murine model of OSA, using cell lines derived from a spontaneously occurring murine OSA (Schmidt et al. Differentiation 1988; 39: 151-60). This model is characterized by orthotopic primary tumor growth, ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1006767007547
更新日期：2000-01-01 00:00:00
abstract：:Development of central nervous system (CNS) metastases in breast cancer (BC) is associated with poor prognosis. The incidence of CNS metastases in metastatic BC is reported to be about 10-16 %, but different subtypes of BC are associated with different risk of developing CNS metastases. We retrospectively analysed the...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-013-9594-5
更新日期：2013-12-01 00:00:00
abstract：:Protein kinase C (PKC) has been shown to be a signal transducer during tumorigenesis, tumor cell invasion, and metastasis. Recent studies have reported that the PKC inhibitor, 7-hydroxystaurosporine, inhibits tumor cell invasion. However, the molecular mechanisms of this inhibition of invasion and metastasis are not w...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9080-z
更新日期：2007-01-01 00:00:00
abstract：:Within the cancer microenvironment, the growth and proliferation of cancer cells in the primary site as well as in the metastatic site represent a global biological phenomenon. To understand the growth, proliferation and progression of cancer either by local expansion and/or metastasis, it is important to understand t...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-018-9921-y
更新日期：2018-08-01 00:00:00
abstract：:Treating metastatic osteosarcoma (OS) remains a challenge in oncology. Current treatment strategies target the primary tumour rather than metastases and have a limited efficacy in the treatment of metastatic disease. Metastatic cells have specific features that render them less sensitive to therapy and targeting these...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1007/s10585-011-9384-x
更新日期：2011-06-01 00:00:00
abstract：:The spinal column is the most frequent site of bone metastasis in patients with breast cancer. It is important to understand how the pattern of vertebral lesions may be affected by the introduction of modern cancer therapies. The purpose of this study was to characterize changes in the radiological appearance of spina...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-008-9219-6
更新日期：2009-01-01 00:00:00
abstract：:Elevated osteopontin (OPN) transcription often correlates with increased metastatic potential of transformed cells, and in several model systems OPN--whether produced by the tumor cells or by stromal cells - has been shown to enhance metastatic ability. Sequence elements in the OPN promoter have been identified on the...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1023/a:1022550721404
更新日期：2003-01-01 00:00:00
abstract：:Metastasis is the most devastating aspect of cancer and it is the main cause of morbidity and mortality in cancer patients. Tumor cell adhesion to the vascular endothelial cell lining is an important step in metastatic progression and is prompted by platelets. Mucin 1 is over-expressed and aberrantly glycosylated in m...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-012-9461-9
更新日期：2012-06-01 00:00:00
abstract：:Vascularization of solid tumors is thought to occur by sprouting or intussusceptive angiogenesis, co-option of existing vessels, and vasculogenic mimicry after the onset of tumor hypoxia, when the tumor radius exceeds the oxygen diffusion distance. In contrast, here we show that individual endothelial cells that are i...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-009-9257-8
更新日期：2009-01-01 00:00:00
abstract：:Basement membrane forms widespread barriers to tumor invasion. It has been shown that tumor-secreted, basement membrane-degrading enzymes, namely metalloproteinases (MMPs) play an important role in tumor invasion and metastasis. In this study, we determined the enzymatic activity, content, and mRNA of both the 72 kDa ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00118181
更新日期：1995-11-01 00:00:00
abstract：:We have compared the pattern of surface antigen expression, as detected by monoclonal antibodies (mAbs), in plasma membranes vs shed membrane vesicles of two human breast carcinoma cell lines, MCF-7 and 8701-BC. Antigen expression was detected on cells by immunofluorescence (IF) analysis, whilst, due to their small di...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF00133483
更新日期：1995-07-01 00:00:00
abstract：:Cancer metastasis shows great diversity in target organs, routes and molecular mechanisms depending on the type of cancer and even on the individual patients. To identify key molecules involved in metastasis, we constructed a murine model system including multiple sublines with different organotropism and pathways of ...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-019-09979-w
更新日期：2019-08-01 00:00:00
abstract：:CNTO 95 is a fully human monoclonal antibody that recognizes alphav integrins. Previous studies have shown that CNTO 95 exhibits both anti-tumor and anti-angiogenic activities (Trikha M et al., Int J Cancer 110:326-335, 2004). In this study we investigated the biological activities of CNTO 95 on breast tumor cells bot...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-007-9132-4
更新日期：2008-01-01 00:00:00
abstract：:The interaction of tumor cells with endothelial cells is a key event in tumor metastasis. We established an in vitro invasion assay system, in which the invasion of tumor cells after interaction with endothelial cells can be examined. Two chamber culture wells separated by porous membrane were used. Human umbilical ve...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/BF01753837
更新日期：1994-07-01 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) is a potent angiogenic growth factor with a key role in many physiological and pathological processes. Investigation into the implications of circulating levels of this cytokine is progressing at an exponential rate. However, there are important inconsistencies between reports...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章,评审
doi：10.1023/a:1021379811308
更新日期：2002-01-01 00:00:00
abstract：:RGD-containing substrates were used to study static and hydrodynamic adhesion of murine RAW117 large-cell lymphoma sublines with differential liver-metastatic potentials. Highly liver-metastatic RAW117-H10 cells had higher rates of static adhesion to vitronectin, fibronectin and (GRGDS)4 than poorly metastatic RAW117-...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1023/a:1018451616309
更新日期：1997-01-01 00:00:00
abstract：:Metastasis, responsible for most deaths from breast cancer (BC), is a multistep process leading to cancer cell spread. Extracellular matrix (ECM)-related adhesion and apoptosis resistance play pivotal role in metastasis. Ras suppressor-1 (RSU-1) localizes to cell-ECM adhesions and binds to pro-survival adhesion protei...
journal_title：Clinical & experimental metastasis
pub_type： 杂志文章
doi：10.1007/s10585-015-9701-x
更新日期：2015-03-01 00:00:00
