Abstract:
:Basement membrane forms widespread barriers to tumor invasion. It has been shown that tumor-secreted, basement membrane-degrading enzymes, namely metalloproteinases (MMPs) play an important role in tumor invasion and metastasis. In this study, we determined the enzymatic activity, content, and mRNA of both the 72 kDa (MMP-2) and 92 kDa (MMP-9) MMPs in primary cultures of human giant-cell tumor of bone (GCT) in vitro and in tissue extracts (in vivo). Gelatin zymography showed the presence of lytic bands at M(r) 121,000, 92,000, and 72,000, and these enzymatic activities were inhibited by EDTA, an inhibitor of MMPs. Western blots with antibodies specific for MMP-2 and MMP-9 confirmed the presence of MMP-2 and MMP-9 both in vitro and in vivo, but GCT cells at late passage showed only MMP-2. Northern blots using labeled cDNA probes specific for these molecules revealed the presence of 3.1 kb transcript for MMP-2 and a 2.9 kb transcript for MMP-9. Using specific antibodies to 72 kDa and 92 kDa type IV collagenases, we studied their cellular distribution by immunohistochemical means. Stronger immunoreactivity was found for 92 kDa type IV collagenase than 72 kDa type IV collagenase in the giant cells. It appears, therefore, that MMP-9 may play an important role in the malignant behavior of GCTs and suggests a potential therapeutic role for protease inhibitors in attempting to minimize the invasive behavior of GCTs.
journal_name
Clin Exp Metastasisjournal_title
Clinical & experimental metastasisauthors
Rao VH,Bridge JA,Neff JR,Schaefer GB,Buehler BA,Vishwanatha JK,Pollock RE,Nicolson GL,Yamamoto M,Gokaslam ZLdoi
10.1007/BF00118181subject
Has Abstract,Author List Incompletepub_date
1995-11-01 00:00:00pages
420-6issue
6eissn
0262-0898issn
1573-7276journal_volume
13pub_type
杂志文章abstract::Two new compounds, 4-[4-[bis-(2-chloroethyl)-amino-]phenyl]-1- hydroxybutane-1,1-bisphosphonic acid (BAD) and aminotris-(methylenephosphonato)-diamminoplatinum(II) (ADP) that both have cytostatic and osteotropic properties, have shown good therapeutic efficacy against an osteosarcoma which metastasizes and kills by lu...
journal_title:Clinical & experimental metastasis
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更新日期:1990-07-01 00:00:00
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journal_title:Clinical & experimental metastasis
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journal_title:Clinical & experimental metastasis
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journal_title:Clinical & experimental metastasis
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journal_title:Clinical & experimental metastasis
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更新日期:1989-07-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF01753679
更新日期:1989-05-01 00:00:00
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journal_title:Clinical & experimental metastasis
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journal_title:Clinical & experimental metastasis
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更新日期:2004-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF00123395
更新日期:1996-09-01 00:00:00
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更新日期:2013-06-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章,评审
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1018470709523
更新日期:1997-09-01 00:00:00
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journal_title:Clinical & experimental metastasis
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更新日期:2009-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
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更新日期:2005-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF01753983
更新日期:1994-03-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-008-9203-1
更新日期:2008-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
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更新日期:2019-08-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF01784377
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journal_title:Clinical & experimental metastasis
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journal_title:Clinical & experimental metastasis
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF00132927
更新日期:1984-07-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/s10585-006-9016-z
更新日期:2006-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
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doi:10.1007/s10585-013-9590-9
更新日期:2013-10-01 00:00:00
abstract::Metastatic variant sublines of the murine RAW117 large cell lymphoma or lymphosarcoma have been established in vitro by sequential cycles of harvesting of liver tumor nodules after intravenous inoculation of tumor cell suspensions into syngeneic BALB/c mice. After five and ten in vivo selections for liver colonization...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF00132928
更新日期:1984-07-01 00:00:00
abstract::A previous study from our laboratory suggested that prostate cancer metastasis to bone may be mediated, in part, by preferential adhesion to human bone marrow endothelial (HBME) cells. Tumor cell adhesion to endothelial cells may be modulated by the effect of cytokines on cell adhesion molecules (CAMs). Tumor necrosis...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1023/a:1013849123736
更新日期:2002-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
doi:10.1007/BF01784333
更新日期:1994-01-01 00:00:00
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journal_title:Clinical & experimental metastasis
pub_type: 杂志文章,评审
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更新日期:2002-01-01 00:00:00
abstract::MicroRNAs have been identified as key players in the development and progression of osteosarcoma, which is the most common primary malignancy of bone. Sequencing-based miR-omic and quantitative real-time PCR analyses suggested that the expression of miR-193a-3p and miR-193a-5p was decreased by DNA methylation at their...
journal_title:Clinical & experimental metastasis
pub_type: 杂志文章
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更新日期:2016-04-01 00:00:00