当前位置： SCI文献检索 > CLINICAL & EXPERIMENTAL METASTASIS期刊下所有文献 > Repetitive and site-specific molecular staging of prostate cancer using nested reverse transcriptase polymerase chain reaction for prostate specific antigen and prostate specific membrane antigen.

Repetitive and site-specific molecular staging of prostate cancer using nested reverse transcriptase polymerase chain reaction for prostate specific antigen and prostate specific membrane antigen.

Abstract：

:We performed repetitive molecular staging using, nested rt-PCR for PSA and PSM at the peripheral blood (PB) and bone marrow (BM) of patients with prostate cancer (Pr.Ca) and benign prostate hyperplasia (BPH) after transrectal ultrasonography-guided biopsy (TRUS-B; 6-9 biopsies/patient), Pr.Ca patients after radical prostatectomy (RP), and Pr.Ca patients with diffuse bony metastases. All BPH patients (N = 20) tested negative at BM. Of the 2 who tested positive at PB 2 weeks after TRUS-B tested negative 8 weeks after TRUS-B. Of the 17 Pr.Ca, 7 (41.2%) tested positive at PB for PSA and PSM 2 weeks after TRUS-B while only 4 (23.5%) of them tested positive at repetitive analysis 8 weeks after TRUS-B. Two (11.8%) of the 17 Pr.Ca patients had positive analysis at BM for PSA and PSM 2 and 8 weeks after TRUS-B. Of 12 Pr.Ca patients with negative pre-operative molecular staging, 7 (58.3%) tested positive at PB for PSA and PSM 2 months post-RP but only 3 (25%) of them re-tested positive 12 months post-RP. Of these 12 Pr.Ca, 4 (33.3%) tested positive at BM for PSA and PSM 2 months post-RP while none re-tested positive 12 months post-RP. All Pr.Ca (N = 20) with diffuse bony lesions tested positive at BM. At PB, 6 of them (30%) tested negative for both PSA and PSM. Our data suggest that nested rt-PCR for PSA and PSM at PB is affected by TRUS-B and RP, while such analysis at BM concerted diffuse bony disease.

journal_name

Clin Exp Metastasis

journal_title

Clinical & experimental metastasis

authors

Koutsilieris M,Lembessis P,Luu-The V,Sourla A

doi

10.1023/a:1006783330996

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

823-30

issue

10

eissn

0262-0898

issn

1573-7276

journal_volume

17

pub_type

杂志文章

相关文献

CLINICAL & EXPERIMENTAL METASTASIS文献大全
  • Improvements in population-based survival of patients presenting with metastatic rectal cancer in the south of the Netherlands, 1992-2008.

    abstract：:We analysed population-based treatment and survival data of patients who presented with metastatic rectal cancer. All patients diagnosed with primary synchronous metastatic rectal cancer between 1992 and 2008 in the Eindhoven Cancer Registry area were included. Date of diagnosis was divided into three periods (1992-19...

    journal_title：Clinical & experimental metastasis

    pub_type： 历史文章,杂志文章

    doi：10.1007/s10585-010-9370-8

    authors： Lemmens VE,de Haan N,Rutten HJ,Martijn H,Loosveld OJ,Roumen RM,Creemers GJ

    更新日期：2011-03-01 00:00:00

  • Molecular phenotype is associated with survival in breast cancer patients with spinal bone metastases.

    abstract：:To aid in therapy selection for patients with spinal bone metastases (SBM), predictive models have been developed. These models consider SBM from breast cancer a positive predictive factor, but do not take phenotypes based on estrogen (ER), progesterone (PR) and human epidermal growth factor 2 (HER2) receptors into ac...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章,多中心研究

    doi：10.1007/s10585-014-9685-y

    authors： Bollen L,Wibmer C,Wang M,van der Linden YM,Leithner A,Bünger CE,Jensen AB,Fiocco M,Bratschitsch G,Pondaag W,Bovée JV,Dijkstra PD

    更新日期：2015-01-01 00:00:00

  • Identification and characterization of motility stimulating factor secreted from pancreatic cancer cells: role in tumor invasion and metastasis.

    abstract：:Cell motility is an important factor in the process of invasion and metastasis of tumor. In this study, the relationship between cell motility and experimental metastatic potential was examined using two human pancreatic cancer cell lines, SW1990 and PANC-1. Serum-free conditioned medium from the highly metastatic cel...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1018429600437

    authors： Sakurai Y,Sawada T,Chung YS,Funae Y,Sowa M

    更新日期：1997-05-01 00:00:00

  • Mapping protein signal pathway interaction in sarcoma bone metastasis: linkage between rank, metalloproteinases turnover and growth factor signaling pathways.

    abstract：:We applied reverse phase protein microarrays technology to map signal pathway interactions in a discovery set of 34 soft tissue sarcoma (STS) bone metastases compared to healthy bone. Proteins associated with matrix remodeling (MMP), adhesion (FAK Y576/577, Syndecan-1), and growth/survival (IGF1R Y1135/1136, PI3K, EGF...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-013-9605-6

    authors： Conti A,Espina V,Chiechi A,Magagnoli G,Novello C,Pazzaglia L,Quattrini I,Picci P,Liotta LA,Benassi MS

    更新日期：2014-01-01 00:00:00

  • The expression of neurokinin-1 and preprotachykinin-1 in breast cancer cells depends on the relative degree of invasive and metastatic potential.

    abstract：:Breast cancer has a predilection for metastasis to the bone marrow. The preprotachykinin-I (PPT-I) gene has a central role in the early migration of breast cancer cells into the bone marrow, making this organ a latent repository of the cancer cells. This study investigated whether the invasive and metastatic potential...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-006-9001-6

    authors： Castro TA,Cohen MC,Rameshwar P

    更新日期：2005-01-01 00:00:00

  • Association of SIBA treatment and a Met-depleted diet inhibits in vitro growth and in vivo metastatic spread of experimental tumor cell lines.

    abstract：:We have used 5'-deoxy-5'-S isobutyl-thioadenosine (SIBA), an analog of S-adenosylhomocysteine, alone or in association with a methionine-depleted diet in order to obtain an antitumoral effect in two different tumor models: a transplantable rat rhabdomyosarcoma (RMS-J1) induced by i.m. injection of nickel and the well-...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/BF01580402

    authors： Breillout F,Poupon MF,Blanchard P,Lascaux V,Echinard-Garin P,Robert-Gero M

    更新日期：1988-01-01 00:00:00

  • Gene transduction of NK4, HGF antagonist, inhibits in vitro invasion and in vivo growth of human pancreatic cancer.

    abstract：:In this study, we investigated the therapeutic effects of adenovirally-mediated transfer of the sequence of NK4, an antagonist for hepatocyte growth factor (HGF), against human pancreatic carcinoma. HGF has been implicated to play an important role in invasion and metastasis of various human cancers through tumor-stro...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1016395316362

    authors： Maehara N,Nagai E,Mizumoto K,Sato N,Matsumoto K,Nakamura T,Narumi K,Nukiwa T,Tanaka M

    更新日期：2002-01-01 00:00:00

  • Effects of dietary fat on spontaneous metastasis of Lewis lung carcinoma in mice.

    abstract：:The present study assessed the effects of dietary fat on spontaneous metastasis of Lewis lung carcinoma in mice. Three-week old male C57BL/6 mice were fed the AIN-93G diet or a 45% fat diet (% kcal.) for 7 weeks before they were subcutaneously injected with 2.5 × 10⁵ viable carcinoma cells. The primary tumor was resec...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-010-9347-7

    authors： Yan L,DeMars LC

    更新日期：2010-12-01 00:00:00

  • The meaning of the c-kit proto-oncogene product in malignant transformation in human mammary epithelium.

    abstract：:To evaluate the relationship between the c-kit proto-oncogene product and malignant transformation of human breast tissue, we examined the immunohistochemical expression of the c-kit proto-oncogene product in both malignant and non-malignant breast tissues. The immunohistochemical expression of the c-kit proto-oncogen...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1027323210736

    authors： Ko CD,Kim JS,Ko BG,Son BH,Kang HJ,Yoon HS,Cho EY,Gong G,Ahn SH

    更新日期：2003-01-01 00:00:00

  • Expression of 72 kDa and 92 kDa type IV collagenases from human giant-cell tumor of bone.

    abstract：:Basement membrane forms widespread barriers to tumor invasion. It has been shown that tumor-secreted, basement membrane-degrading enzymes, namely metalloproteinases (MMPs) play an important role in tumor invasion and metastasis. In this study, we determined the enzymatic activity, content, and mRNA of both the 72 kDa ...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/BF00118181

    authors： Rao VH,Bridge JA,Neff JR,Schaefer GB,Buehler BA,Vishwanatha JK,Pollock RE,Nicolson GL,Yamamoto M,Gokaslam ZL

    更新日期：1995-11-01 00:00:00

  • MMP-9 secretion and MMP-2 activation distinguish invasive and metastatic sublines of a mouse mammary carcinoma system showing epithelial-mesenchymal transition traits.

    abstract：:We have investigated the gelatinase profiles and invasiveness of clonal tumour sublines derived from a spontaneously arising mammary tumour in a Balb/cfC3H mouse. The 67NR. 66c14 and 4T1.2 sublines have low, intermediate and high metastatic potential respectively. In Boyden chamber studies, Matrigel invasion was seen ...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1011953118186

    authors： Tester AM,Ruangpanit N,Anderson RL,Thompson EW

    更新日期：2000-01-01 00:00:00

  • The invasive and metastatic properties of hormone-independent but hormone-responsive variants of MCF-7 human breast cancer cells.

    abstract：:We have previously isolated a series of MCF-7 human breast cancer cell variants which no longer require estrogen-supplementation for tumor growth in nude mice (Clarke et al. Proc Natl Acad Sci USA 86: 3649-3653, 1989). We now report that these hormone-independent and hormone-responsive variants (MIII, MCF7/LCC1) can i...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/BF00880062

    authors： Thompson EW,Brünner N,Torri J,Johnson MD,Boulay V,Wright A,Lippman ME,Steeg PS,Clarke R

    更新日期：1993-01-01 00:00:00

  • Inhibitory effect of non-anticoagulant heparin (S-NACH) on pancreatic cancer cell adhesion and metastasis in human umbilical cord vessel segment and in mouse model.

    abstract：:Metastasis is the most devastating aspect of cancer and it is the main cause of morbidity and mortality in cancer patients. Tumor cell adhesion to the vascular endothelial cell lining is an important step in metastatic progression and is prompted by platelets. Mucin 1 is over-expressed and aberrantly glycosylated in m...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-012-9461-9

    authors： Sudha T,Phillips P,Kanaan C,Linhardt RJ,Borsig L,Mousa SA

    更新日期：2012-06-01 00:00:00

  • Transcriptional profiling identifies genes induced by hepatocyte-derived extracellular matrix in metastatic human colorectal cancer cell lines.

    abstract：:The milieu of the liver, and in particular hepatocyte-derived extracellular matrix (hECM), is a critical factor regulating development of liver metastases of colorectal cancer (CRC) cells. The present study has investigated genes altered by hECM in CRC cells and particularly by heparan sulfate chains of hepatocyte pro...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-012-9527-8

    authors： Zvibel I,Wagner A,Pasmanik-Chor M,Varol C,Oron-Karni V,Santo EM,Halpern Z,Kariv R

    更新日期：2013-02-01 00:00:00

  • Radiobiology and radiotherapy of brain metastases.

    abstract：:Brain metastases are the most common intracranial tumors in adults, accounting for more than 50% of all such cases. The approach to and management of brain metastases have evolved significantly in recent years due to several reasons. These include advances in neurosurgical and radiotherapeutic techniques, improved sys...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章,评审

    doi：10.1007/s10585-017-9865-7

    authors： Thiagarajan A,Yamada Y

    更新日期：2017-10-01 00:00:00

  • Dunning rat prostate adenocarcinomas and alternative splicing reporters: powerful tools to study epithelial plasticity in prostate tumors in vivo.

    abstract：:Using alternative splicing reporters we have previously observed mesenchymal epithelial transitions in Dunning AT3 rat prostate tumors. We demonstrate here that the Dunning DT and AT3 cells, which express epithelial and mesenchymal markers, respectively, represent an excellent model to study epithelial transitions sin...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-008-9186-y

    authors： Oltean S,Febbo PG,Garcia-Blanco MA

    更新日期：2008-01-01 00:00:00

  • Impact of decompressive laminectomy on the functional outcome of patients with metastatic spinal cord compression and neurological impairment.

    abstract：:Metastatic spinal cord compression (MSCC) is a frequent phenomenon in advanced tumor diseases with often severe neurological impairments. Affected patients are often treated by decompressive laminectomy. To assess the impact of this procedure on Karnofsky Performance Index (KPI) and Frankel Grade (FG) at discharge, a ...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-019-10016-z

    authors： Younsi A,Riemann L,Scherer M,Unterberg A,Zweckberger K

    更新日期：2020-04-01 00:00:00

  • Phenotypic and functional characteristics of tumour-derived microvascular endothelial cells.

    abstract：:We recently developed a method for the isolation and purification of tumour-derived endothelium. In this study the phenotypic and functional properties of human tumour-derived microvascular endothelial cells (TdMEC) were examined. Endothelium obtained from human adrenal gland specimens (HAMEC) was used as a reference ...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1006738901839

    authors： Alessandri G,Chirivi RG,Fiorentini S,Dossi R,Bonardelli S,Giulini SM,Zanetta G,Landoni F,Graziotti PP,Turano A,Caruso A,Zardi L,Giavazzi R,Bani MR

    更新日期：1999-01-01 00:00:00

  • Enhanced cell surface CD44 variant (v6, v9) expression by osteopontin in breast cancer epithelial cells facilitates tumor cell migration: novel post-transcriptional, post-translational regulation.

    abstract：:Osteopontin (OPN) is a glycosylated, secreted phosphoprotein that functions both as a cell attachment and chemotactic factor. Elevated expression of OPN confers enhanced metastatic ability on transformed cells, suggesting that OPN may contribute to the malignant progression of tumors. Migration of mammary carcinoma ce...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-006-9007-0

    authors： Khan SA,Cook AC,Kappil M,Günthert U,Chambers AF,Tuck AB,Denhardt DT

    更新日期：2005-01-01 00:00:00

  • An orthotopic model of murine osteosarcoma with clonally related variants differing in pulmonary metastatic potential.

    abstract：:To provide an investigative tool for the study of osteosarcoma (OSA) biology we have developed a syngeneic (balb/c) murine model of OSA, using cell lines derived from a spontaneously occurring murine OSA (Schmidt et al. Differentiation 1988; 39: 151-60). This model is characterized by orthotopic primary tumor growth, ...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1006767007547

    authors： Khanna C,Prehn J,Yeung C,Caylor J,Tsokos M,Helman L

    更新日期：2000-01-01 00:00:00

  • Different sialyltransferase activities in human colorectal carcinoma cells from surgical specimens detected by specific glycoprotein and glycolipid acceptors.

    abstract：:The amount and type of sialylation of tumor cell membranes depends on the activity of a number of different sialyltransferase enzymes. For the detection of specific activities in human colorectal carcinoma tissue several glycoprotein and glycolipid acceptors were used: desialylated fetuin, alpha 1-acid glycoprotein, b...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/BF01753893

    authors： Kemmner W,Krück D,Schlag P

    更新日期：1994-05-01 00:00:00

  • Control pathways of the 67 kDa laminin binding protein: surface expression and activity of a new ligand binding domain.

    abstract：:A number of papers have been published on the clinical correlation of the expression of the 67 kDa laminin binding protein (LBP) with the metastatic potential of solid tumors. Both mRNA and protein expression levels have been reported, but both the relationship between them and the molecular nature of the 67 kDa surfa...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/BF00121912

    authors： Landowski TH,Uthayakumar S,Starkey JR

    更新日期：1995-09-01 00:00:00

  • Experimental liver metastasis: standards for local cell implantation to study isolated tumor growth in mice.

    abstract：:Experimental hepatic metastasis of colorectal tumors is frequently studied by local intrahepatic tumor cell implantation. However, although a variety of factors of the implantation procedure may markedly influence tumor growth characteristics, standards are not defined yet. Herein, we studied the effect of different m...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-004-2696-3

    authors： Kollmar O,Schilling MK,Menger MD

    更新日期：2004-01-01 00:00:00

  • Differential adhesion of metastatic RAW117 large-cell lymphoma cells under static or hydrodynamic conditions: role of integrin alpha(v) beta3.

    abstract：:RGD-containing substrates were used to study static and hydrodynamic adhesion of murine RAW117 large-cell lymphoma sublines with differential liver-metastatic potentials. Highly liver-metastatic RAW117-H10 cells had higher rates of static adhesion to vitronectin, fibronectin and (GRGDS)4 than poorly metastatic RAW117-...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1018451616309

    authors： Yun Z,Smith TW,Menter DG,McIntire LV,Nicolson GL

    更新日期：1997-01-01 00:00:00

  • Preoperative sentinel lymph node mapping of the prostate using PET/CT fusion imaging and Ga-68-labeled tilmanocept in an animal model.

    abstract：:Sentinel lymph node (SLN) identification with preoperative image guidance may improve pathological staging of prostate cancer by identifying nodes outside the standard template of dissection. Four anesthetized male dogs received an intra-prostatic injection of Ga-68-labeled tilmanocept. Every 20 min the pelvic lymph n...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-012-9498-9

    authors： Stroup SP,Kane CJ,Farchshchi-Heydari S,James CM,Davis CH,Wallace AM,Hoh CK,Vera DR

    更新日期：2012-10-01 00:00:00

  • An examination of the effects of hypoxia, acidosis, and glucose starvation on the expression of metastasis-associated genes in murine tumor cells.

    abstract：:Tumor cells exposed to a growth stress such as low pH, glucose starvation and hypoxia have been shown to exhibit a transient increase in experimental metastatic potential, particularly when allowed to recover under normal growth conditions for a period of 24-48 h. In this study we examined whether this increase in met...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1023/a:1018470709523

    authors： Jang A,Hill RP

    更新日期：1997-09-01 00:00:00

  • Circulating microRNAs in head and neck cancer: a scoping review of methods.

    abstract：:Circulating microRNAs have been described as head and neck cancer biomarkers in multiple anatomical subsites including the oral cavity, nasopharynx, larynx, salivary glands and the skin. While there is an expanding volume of published literature showing the significance of individual or panels of microRNAs, the clinic...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章,评审

    doi：10.1007/s10585-019-09961-6

    authors： Dharmawardana N,Ooi EH,Woods C,Hussey D

    更新日期：2019-06-01 00:00:00

  • Site-dependence of invasiveness of ECA109 human oesophageal carcinoma cells in nude mice.

    abstract：:ECA109 human oesophageal carcinoma cells were injected either subcutaneously or intraperitoneally into BALB/CATc 1-nu/nu mice. After 23 weeks tumours were examined histologically and by scanning electron microscopy. Subcutaneous ECA109 tumours were well-delineated without signs of invasion. By contrast, intra-abdomina...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/BF00132927

    authors： Gao J,Xue KX,Li BG,Dong HY,Wei LS,Zhang ZH,Tian S,Xu ZG

    更新日期：1984-07-01 00:00:00

  • Metastasis of mammary tumours in mice: relationship with morphology of primary tumour, and reproductive background of host.

    abstract：:The lungs of BR6 breeding female mice which had developed spontaneous mammary tumours, were examined histologically for the presence of metastases. Metastatic nodules, or tumour cell emboli within blood vessels, were found in the lungs of 3 out of 42 (7 per cent) mice with pregnancy-dependent tumours, and in 33 out of...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/BF00736406

    authors： Lee AE,Pang LS,Rogers LA,Jeffery RE

    更新日期：1983-07-01 00:00:00

  • Automated CT-based analysis to detect changes in the prevalence of lytic bone metastases from breast cancer.

    abstract：:The spinal column is the most frequent site of bone metastasis in patients with breast cancer. It is important to understand how the pattern of vertebral lesions may be affected by the introduction of modern cancer therapies. The purpose of this study was to characterize changes in the radiological appearance of spina...

    journal_title：Clinical & experimental metastasis

    pub_type： 杂志文章

    doi：10.1007/s10585-008-9219-6

    authors： Skrinskas T,Clemons M,Freedman O,Weller I,Whyne CM

    更新日期：2009-01-01 00:00:00