Potentiation of cell invasion and matrix metalloproteinase production by alpha3beta1 integrin-mediated adhesion of gastric carcinoma cells to laminin-5.


:We previously reported that the adhesion of gastric carcinoma cells to the peritoneum mediated by the alpha3beta1 integrin-laminin interaction is a key step in the initial process of peritoneal metastatic dissemination. Carcinoma cells subsequently invade through the intercellular gaps of mesothelial linings. In this study, we examined the role of the interaction of carcinoma cells with laminin-5, which is a major component of submesothelial basement membranes and serves as a high-affinity ligand for alpha3beta1 integrin, in carcinoma cell invasion. Human gastric carcinoma cell lines (MKN1, GT3TKB, and NUGC-4) adhered in an alpha3beta1 integrin-dependent manner to the extracellular matrix deposited by peritoneal mesothelial cells. An in vitro invasion assay using the Boyden chamber system revealed that MKN1 cell migration through the membranes increased when the membranes were coated with matrices produced by mesothelial cells or with laminin-5-containing Matrigel as compared to Matrigel alone. The cell migration promoted by laminin-5-containing Matrigel was inhibited by the presence of anti-alpha3 integrin antibody. When MKN1 cells were cultured in a laminin-5-coated plate, these cells were promoted to produce matrix metalloproteinase (MMP)-9, as assessed by gelatin zymography, enzyme-linked immunosorbent assay, and reverse transcription-polymerase chain reaction. These results suggest that the production of MMP-9 by MKN1 cells was potentiated by the alpha3beta1 integrin-laminin-5 interaction, which facilitated their invasion via degradation of the matrix.


Clin Exp Metastasis


Saito Y,Sekine W,Sano R,Komatsu S,Mizuno H,Katabami K,Shimada K,Oku T,Tsuji T




Has Abstract


2010-04-01 00:00:00












  • Intracellular esterase activity in living cells may distinguish between metastatic and tumor-free lymph nodes.

    abstract:BACKGROUND:One of the major clinical problems in breast cancer detection is the relatively high incidence of occult lymph node metastases undetectable by standard procedures. Since the ascertainment of breast cancer stage determines the following treatment, such a "hypo-diagnosis" leads to inadequate therapy, and hence...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Afrimzon E,Deutsch A,Shafran Y,Zurgil N,Sandbank J,Pappo I,Deutsch M

    更新日期:2008-01-01 00:00:00

  • Expression of activated gelatinase in human invasive breast carcinoma.

    abstract::The expression of both 92- and 72-kDa gelatinases has been studied in 20 samples of human breast carcinoma by the technique of gelatin zymography. This technique allowed the relative amount of each gelatinase to be determined in small samples of tissue (< 10 mg). More importantly, active and latent forms of the two ge...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Brown PD,Bloxidge RE,Anderson E,Howell A

    更新日期:1993-03-01 00:00:00

  • The role of intraperitoneal chemotherapy in the surgical management of pancreatic ductal adenocarcinoma: a systematic review.

    abstract::Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy with poor prognosis, particularly for patients with metastatic disease. Treatment for oligometastatic presentation has been reported in recent literature, but the role of intraperitoneal chemotherapy for patients with peritoneal metastases (PM) remain...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Brind'Amour A,Webb M,Parapini M,Sidéris L,Segedi M,Chung SW,Chartier-Plante S,Dubé P,Scudamore CH,Kim PTW

    更新日期:2021-01-24 00:00:00

  • Predicting brain metastases for non-small cell lung cancer based on magnetic resonance imaging.

    abstract::In this study the relationship between brain structure and brain metastases (BM) occurrence was analyzed. A model for predicting the time of BM onset in patients with non-small cell lung cancer (NSCLC) was proposed. Twenty patients were used to develop the model, whereas the remaining 69 were used for independent vali...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Yin G,Li C,Chen H,Luo Y,Orlandini LC,Wang P,Lang J

    更新日期:2017-02-01 00:00:00

  • Distinctive alterations of invasiveness, drug resistance and cell-cell organization in 3D-cultures of MCF-7, a human breast cancer cell line, and its multidrug resistant variant.

    abstract::Growth of human tumor cells as three-dimensional (3D) multicellular spheroids modifies their invasive properties. Here we study the differences in the biological features of MCF-7, a human breast cancer cell line, and its multidrug resistant variant (MDR-MCF-7) cultured as spheroids or as monolayers. Three-dimensional...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: dit Faute MA,Laurent L,Ploton D,Poupon MF,Jardillier JC,Bobichon H

    更新日期:2002-01-01 00:00:00

  • GPR56 plays varying roles in endogenous cancer progression.

    abstract::GPR56, a non-classical adhesion receptor, was previously reported to suppress tumor growth and metastasis in xenograft models using human melanoma cell lines. To understand whether GPR56 plays similar roles in the development of endogenous tumors, we analyzed cancer progression in Gpr56 (-/-) mice using a variety of t...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Xu L,Begum S,Barry M,Crowley D,Yang L,Bronson RT,Hynes RO

    更新日期:2010-04-01 00:00:00

  • Matrix metalloproteinase 9 is induced by the Epstein-Barr virus BZLF1 transactivator.

    abstract::Type IV collagenases, matrix metalloproteinase (MMP) 2 and MMP9 are implicated in tumor invasion and metastasis. In patients with nasopharyngeal carcinoma (NPC), poor prognosis due to development of local and distant metastasis has been reported to be predicted by antibody titers against the Z protein which is an AP-1...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Yoshizaki T,Sato H,Murono S,Pagano JS,Furukawa M

    更新日期:1999-07-01 00:00:00

  • Automated CT-based analysis to detect changes in the prevalence of lytic bone metastases from breast cancer.

    abstract::The spinal column is the most frequent site of bone metastasis in patients with breast cancer. It is important to understand how the pattern of vertebral lesions may be affected by the introduction of modern cancer therapies. The purpose of this study was to characterize changes in the radiological appearance of spina...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Skrinskas T,Clemons M,Freedman O,Weller I,Whyne CM

    更新日期:2009-01-01 00:00:00

  • A novel in vitro assay system for transendothelial tumor cell invasion: significance of E-selectin and alpha 3 integrin in the transendothelial invasion by HT1080 fibrosarcoma cells.

    abstract::The interaction of tumor cells with endothelial cells is a key event in tumor metastasis. We established an in vitro invasion assay system, in which the invasion of tumor cells after interaction with endothelial cells can be examined. Two chamber culture wells separated by porous membrane were used. Human umbilical ve...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Okada T,Okuno H,Mitsui Y

    更新日期:1994-07-01 00:00:00

  • Mitogen activated protein kinase pathway is involved in RhoC GTPase induced motility, invasion and angiogenesis in inflammatory breast cancer.

    abstract::Inflammatory breast cancer (IBC) is the most lethal form of locally advanced breast cancer known. IBC carries a guarded prognosis primarily due to rapid onset of disease, typically within six months, and the propensity of tumor emboli to invade the dermal lymphatics and spread systemically. Although the clinical manif...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: van Golen KL,Bao LW,Pan Q,Miller FR,Wu ZF,Merajver SD

    更新日期:2002-01-01 00:00:00

  • Angiogenesis and the role of epigenetics in metastasis.

    abstract::The major obstacle to devising effective ways to treat cancer is its heterogeneity and genetic instability. It was originally postulated that targeting the process of tumor angiogenesis could circumvent this problem, as it involves genetically stable epigenetically controlled host stroma. Thus, anti-angiogenic approac...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Coomber BL,Yu JL,Fathers KE,Plumb C,Rak JW

    更新日期:2003-01-01 00:00:00

  • The expression of neurokinin-1 and preprotachykinin-1 in breast cancer cells depends on the relative degree of invasive and metastatic potential.

    abstract::Breast cancer has a predilection for metastasis to the bone marrow. The preprotachykinin-I (PPT-I) gene has a central role in the early migration of breast cancer cells into the bone marrow, making this organ a latent repository of the cancer cells. This study investigated whether the invasive and metastatic potential...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Castro TA,Cohen MC,Rameshwar P

    更新日期:2005-01-01 00:00:00

  • Thymosin beta15 expression in tumor cell lines with varying metastatic potential.

    abstract::We previously isolated thymosin beta15 from highly metastatic Dunning rat prostatic carcinoma cells. Immunohistochemical study of human prostate cancer specimens revealed a general correlation between Gleason grade and thymosin beta15 expression, with high-grade (more malignant) tumors showing increased staining compa...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Bao L,Loda M,Zetter BR

    更新日期:1998-04-01 00:00:00

  • Ras suppressor-1 promotes apoptosis in breast cancer cells by inhibiting PINCH-1 and activating p53-upregulated-modulator of apoptosis (PUMA); verification from metastatic breast cancer human samples.

    abstract::Metastasis, responsible for most deaths from breast cancer (BC), is a multistep process leading to cancer cell spread. Extracellular matrix (ECM)-related adhesion and apoptosis resistance play pivotal role in metastasis. Ras suppressor-1 (RSU-1) localizes to cell-ECM adhesions and binds to pro-survival adhesion protei...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Giotopoulou N,Valiakou V,Papanikolaou V,Dubos S,Athanassiou E,Tsezou A,Zacharia LC,Gkretsi V

    更新日期:2015-03-01 00:00:00

  • SCUBE3 regulation of early lung cancer angiogenesis and metastatic progression.

    abstract::Signal peptide-CUB-EGF-like domain-containing protein 3 (SCUBE3) is strongly expressed in extremely invasive lung carcinoma. We showed in our previous study that SCUBE3 triggers the transforming growth factor-β pathway and subsequently promotes tumor angiogenesis and the epithelial-mesenchymal transition (EMT). Howeve...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Chou CH,Cheng YF,Siow TY,Kumar A,Peck K,Chang C

    更新日期:2013-08-01 00:00:00

  • Plasma basic fibroblast growth factor levels in colorectal cancer: a clinically useful assay?

    abstract::Angiogenic cytokines in the plasma and serum of cancer patients may serve as 'surrogate' markers of tumour neoangiogenesis. Serum VEGF correlates with disease stage in colorectal cancer (CRC), but the role of bFGF in CRC is uncertain. This study aimed to assess plasma bFGF levels in CRC patients before treatment, duri...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: George ML,Tutton MG,Abulafi AM,Eccles SA,Swift RI

    更新日期:2002-01-01 00:00:00

  • Human and rat glioma growth, invasion, and vascularization in a novel chick embryo brain tumor model.

    abstract::The mechanisms that control the insidiously invasive nature of malignant gliomas are poorly understood, and their study would be facilitated by an in vivo model that is easy to manipulate and inexpensive. The developing chick embryo brain was assessed as a new xenograft model for the production, growth, and study of h...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Cretu A,Fotos JS,Little BW,Galileo DS

    更新日期:2005-01-01 00:00:00

  • Functional blocking of specific integrins inhibit colonic cancer migration.

    abstract::For more effective oncological management of disseminated colorectal cancer, therapies must be devised that target the different individual stages of metastasis development. Recent work showed that integrin subunits alpha2, alpha6 and beta4 are involved in the colorectal cancer cell extravasation process. By means of ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Robertson JH,Yang SY,Winslet MC,Seifalian AM

    更新日期:2009-01-01 00:00:00

  • Establishment of highly metastatic tumor clones derived from a skin squamous cell carcinoma (SqC-NH), D-1 and F-3, with distinct features of pulmonary metastases.

    abstract::We have established two highly metastatic tumor clones, D-1 and F-3, which have the distinct features of undergoing pulmonary metastases. Both clones were derived from a skin squamous cell carcinoma (SqC-NH), which had spontaneously occurred in DS-Nh mice. F-3 was morphologically spindle-shaped in tumor mass and adher...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Maekawa R,Kishimoto Y,Sato K,Homma M

    更新日期:1993-07-01 00:00:00

  • Association of SIBA treatment and a Met-depleted diet inhibits in vitro growth and in vivo metastatic spread of experimental tumor cell lines.

    abstract::We have used 5'-deoxy-5'-S isobutyl-thioadenosine (SIBA), an analog of S-adenosylhomocysteine, alone or in association with a methionine-depleted diet in order to obtain an antitumoral effect in two different tumor models: a transplantable rat rhabdomyosarcoma (RMS-J1) induced by i.m. injection of nickel and the well-...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Breillout F,Poupon MF,Blanchard P,Lascaux V,Echinard-Garin P,Robert-Gero M

    更新日期:1988-01-01 00:00:00

  • The role of the spleen in the organ-specific metastasis of murine BW 5147 T lymphomas.

    abstract::Organ-specific metastasis of tumour cells may result from selective invasion and growth or from selective host cell responses. The present study demonstrates how selective interactions with the host affect the metastatic pattern of two murine T cell hybridoma lines, derived from the BW 5147 thymoma. Upon intravenous i...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Schmidt C,Verschueren H,Toussaint-Demylle D,van den Berg T,Kraal G,De Baetselier P

    更新日期:1994-03-01 00:00:00

  • The meaning of the c-kit proto-oncogene product in malignant transformation in human mammary epithelium.

    abstract::To evaluate the relationship between the c-kit proto-oncogene product and malignant transformation of human breast tissue, we examined the immunohistochemical expression of the c-kit proto-oncogene product in both malignant and non-malignant breast tissues. The immunohistochemical expression of the c-kit proto-oncogen...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Ko CD,Kim JS,Ko BG,Son BH,Kang HJ,Yoon HS,Cho EY,Gong G,Ahn SH

    更新日期:2003-01-01 00:00:00

  • Methylation-mediated downregulation of long noncoding RNA LOC100130476 in gastric cardia adenocarcinoma.

    abstract::Accumulating evidences indicate that long non-coding RNAs (lncRNAs) play important roles in several biological processes and dysregulated lncRNAs are involved in different kinds of cancer and are associated with carcinogenesis, metastasis, and prognosis of cancer. The role of a new lncRNA LOC100130476 in gastric cardi...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Guo W,Dong Z,Shi Y,Liu S,Liang J,Guo Y,Guo X,Shen S,Wang G

    更新日期:2016-06-01 00:00:00

  • Evaluation of the interaction between TGF beta and nitric oxide in the mechanisms of progression of colon carcinoma.

    abstract::It is recognised that stromal cells determine cancer progression. We have previously shown that active TGFbeta produced by rat colon carcinoma cells modulated NO production in rat endothelial cells. To elucidate the role of TGFbeta and NO in the mechanisms of interaction of colon carcinoma cells with stromal cells and...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Lohm S,Peduto-Eberl L,Lagadec P,Renggli-Zulliger N,Dudler J,Jeannin JF,Juillerat-Jeanneret L

    更新日期:2005-01-01 00:00:00

  • Soft tissue invasion of papillary thyroid carcinoma.

    abstract::Extrathyroidal extension (ETE) of papillary thyroid carcinoma (PTC) is common and clinical presentation can vary from minimal to extensive locoregional involvement. Although PTC is generally considered the most benign among all thyroid carcinomas, it may present with local invasion with poor prognosis. Our retrospecti...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Lin JD,Hsueh C,Chao TC

    更新日期:2016-08-01 00:00:00

  • Neurosurgical management of brain metastases.

    abstract::Brain metastases present a significant public health issue, affecting more than 100,000 patients per year in the U.S. and result in significant morbidity. Brain metastases can occur in a variety of clinical situations ranging from multiple brain metastases with uncontrolled systemic disease to a solitary metastasis in...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Ferguson SD,Wagner KM,Prabhu SS,McAleer MF,McCutcheon IE,Sawaya R

    更新日期:2017-10-01 00:00:00

  • Meeting the biologic challenge of colorectal metastases.

    abstract::An overview of colorectal cancer discussed (Philip Paty) the good outcome after primary management with local control in 90-95 % of colon and 85 % in rectal cancer patients with major progression to metastases and to death related to hematogenous dissemination. The major disease pathways include the APC, aneuploid pat...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章,评审


    authors: Wanebo HJ,LeGolvan M,Paty PB,Saha S,Zuber M,D'Angelica MI,Kemeny NE

    更新日期:2012-10-01 00:00:00

  • Leading malignant cells initiate collective epithelial cell invasion in a three-dimensional heterotypic tumor spheroid model.

    abstract::Solid tumors consist of genetically and phenotypically diverse subpopulations of cancer cells with unique capacities for growth, differentiation, and invasion. While the molecular and microenvironmental bases for heterogeneity are increasingly appreciated, the outcomes of such intratumor heterogeneity, particularly in...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Carey SP,Starchenko A,McGregor AL,Reinhart-King CA

    更新日期:2013-06-01 00:00:00

  • MiR-193a-3p and miR-193a-5p suppress the metastasis of human osteosarcoma cells by down-regulating Rab27B and SRR, respectively.

    abstract::MicroRNAs have been identified as key players in the development and progression of osteosarcoma, which is the most common primary malignancy of bone. Sequencing-based miR-omic and quantitative real-time PCR analyses suggested that the expression of miR-193a-3p and miR-193a-5p was decreased by DNA methylation at their...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Pu Y,Zhao F,Cai W,Meng X,Li Y,Cai S

    更新日期:2016-04-01 00:00:00

  • Influence of adoptively transferred thioglycollate-elicited peritoneal macrophages on metastasis formation in mice with depressed or stimulated NK activity.

    abstract::The effect of thioglycollate-elicited macrophages (TG-M phi) on natural killer (NK)-cell activity and metastases formation in mice was investigated. Intravenously (i.v.) inoculated TG-M phi inhibited spleen NK activity of normal mice and abrogated polyinosinic: polycytidylic (poly I:C) induced augmentation of NK cell ...

    journal_title:Clinical & experimental metastasis

    pub_type: 杂志文章


    authors: Gorelik E,Wiltrout RH,Brunda MJ,Bere WE,Herberman RB

    更新日期:1985-04-01 00:00:00