Abstract:
:Although fluorescence microscopy has proven to be one of the most powerful tools in biology, its application to the intact animal has been limited to imaging several hundred micrometers below the surface. The rest of the animal has eluded investigation at the microscopic level without excising tissue or performing extensive surgery. However, the ability to image with subcellular resolution in the intact animal enables a contextual setting that may be critical for understanding proper function. Clinical applications such as disease diagnosis and optical biopsy may benefit from minimally invasive in vivo approaches. Gradient index (GRIN) lenses with needle-like dimensions can transfer high-quality images many centimeters from the object plane. Here, we show that multiphoton microscopy through GRIN lenses enables minimally invasive, subcellular resolution several millimeters in the anesthetized, intact animal, and we present in vivo images of cortical layer V and hippocampus in the anesthetized Thy1-YFP line H mouse. Microangiographies from deep capillaries and blood vessels containing fluorescein-dextran and quantum dot-labeled serum in wild-type mouse brain are also demonstrated.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Levene MJ,Dombeck DA,Kasischke KA,Molloy RP,Webb WWdoi
10.1152/jn.01007.2003subject
Has Abstractpub_date
2004-04-01 00:00:00pages
1908-12issue
4eissn
0022-3077issn
1522-1598pii
01007.2003journal_volume
91pub_type
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