Abstract:
:1. Voltage-dependent Na+ conductances were studied in small (18-25 microns diam) adult rat dorsal root ganglion (DRG) neurons with the use of the whole cell patch-clamp technique. Na+ currents were also recorded from larger (44-50 microns diam) neurons and compared with those of the small neurons. 2. The predominant Na+ conductance in the small neurons was selective over tetramethylammonium by at least 10-fold and was resistant to 1 microM external tetrodotoxin (TTX). Na+ conductances in many larger DRG neurons were kinetically faster and, in contrast, were blocked by 1 microM TTX. 3. The Na+ conductance in the small neurons was kinetically slow. Activation half-times were voltage dependent and ranged from 2 ms at -20 mV to 0.7 ms at +50 mV. Approximately 50% of the activation half-time was comprised of an initial delay. Inactivation half-times were voltage dependent and ranged from 11 ms at -20 mV to 2 ms at +50 mV. 4. Peak slow Na+ conductances were near maximal with conditioning potentials negative to -120 mV and were significantly reduced or eliminated with conditioning potentials positive to -40 mV. The slow Na+ conductance increased gradually with test potentials extending from -40 to +40 mV. In some cells the conductance could be saturated at +10 mV. Peak conductance/voltage relationships, although stable in a given neuron, revealed marked variability among neurons, spanning > 20- and 50-mV domains for steady-state activation and inactivation (current availability), respectively. 5. Kinetics remained stable within a given neuron over the course of an experiment. However, considerable kinetic variation was exhibited from neuron to neuron, such that the half-times of activation and of inactivation spanned an order of magnitude. In all small neurons studied there appeared to be a singular kinetic component of the current, based on sensitivity to the conditioning potential, voltage dependence of activation, and inactivation half-time. 6. Unique closing properties were exhibited by Na+ channels of the small neurons. Hyperpolarization following a depolarization-induced fully inactivated state resulted in tail currents that appeared to be the consequence of reactivation of the slow Na+ conductance. Tail currents recorded at various times during a fixed level of depolarization revealed that the underlying channels accumulated into a volatile inactivated state over the course of the preceding depolarization.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Rizzo MA,Kocsis JD,Waxman SGdoi
10.1152/jn.1994.72.6.2796subject
Has Abstractpub_date
1994-12-01 00:00:00pages
2796-815issue
6eissn
0022-3077issn
1522-1598journal_volume
72pub_type
杂志文章abstract::The responses of cortical neurons to repeated presentation of a stimulus are highly variable, yet correlated. These "noise correlations" reflect a low-dimensional structure of population dynamics. Here, we examine noise correlations in 22,705 pairs of neurons in primary visual cortex (V1) of anesthetized cats, during ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00094.2015
更新日期:2015-08-01 00:00:00
abstract::One-hertz repetitive transcranial magnetic stimulation (1Hz-rTMS) over ipsilateral motor cortex is able to modify up to 30 min the motor performance of repetitive finger opposition movements paced with a metronome at 2 Hz. We investigated whether the long-lasting rTMS effect on motor behavior can be modulated by subse...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.90428.2008
更新日期:2009-03-01 00:00:00
abstract::1. Opaline release in Aplysia provides a simple model system for examining the biochemical and electrophysiological mechanisms underlying glandular secretion and its modulation. The opaline gland is a large multivesicular structure, which is innervated by at least three large identified motor neurons located within th...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1982.48.6.1347
更新日期:1982-12-01 00:00:00
abstract::Mutual inhibition between the GABAergic cells of the thalamic reticular nucleus (RTN) is important in regulating oscillations in the thalamocortical network, promoting those in the spindle range of frequencies over those at lower frequencies. Excitatory inputs to the RTN from the cerebral cortex are numerically large ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00624.2003
更新日期:2004-02-01 00:00:00
abstract::The ability of a spatial population of cutaneous, Adelta, and C mechano-nociceptors to encode the location and intensity of a noxious, cutaneous indentation was examined using an isolated preparation in a rat model. Skin and its intact innervation were harvested from the medial thigh of the rat hindlimb and placed in ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.83.5.3049
更新日期:2000-05-01 00:00:00
abstract::1. Adult rats and rats with a postnatal age of 3-29 days (PN 3-29) were used for the preparation of in vitro slices of the frontal neocortex. Epileptiform activity was induced by bath application of the gamma-aminobutyric acid-A (GABAA) receptor antagonists bicuculline or picrotoxin. 2. The voltage-sensitive dye RH 41...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.72.4.1756
更新日期:1994-10-01 00:00:00
abstract::Using intrinsic signal optical imaging (ISI), we have shown previously that the point spread of evoked activity in the rat barrel cortex in response to single-whisker stimulation encompasses a surprisingly large area. Given that our typical stimulation consists of five deflections at 5 Hz, the large area of evoked act...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1999.81.2.692
更新日期:1999-02-01 00:00:00
abstract::Responses to tones, clicks, and noise were recorded from chinchilla auditory-nerve fibers (ANFs). The responses to noise were analyzed by computing the zeroth-, first-, and second-order Wiener kernels (h0, h1, and h2). The h1s correctly predicted the frequency tuning and phases of responses to tones of ANFs with low c...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00885.2004
更新日期:2005-06-01 00:00:00
abstract::Monoamines are strong modulators and/or activators of spinal locomotor networks. Thus monoaminergic fibers likely contact neurons involved in generating locomotion. The aim of the present study was to investigate the serotonergic innervation of locomotor-activated neurons within the thoraco-lumbar spinal cord followin...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.91179.2008
更新日期:2009-09-01 00:00:00
abstract::Successful negotiation of obstacles during walking relies on the integration of visual information about the environment with ongoing locomotor commands. When information about the body and the environment is removed through occlusion of the lower visual field, individuals increase downward head pitch angle, reduce fo...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00931.2017
更新日期:2018-08-01 00:00:00
abstract::1. The effects of dorsal raphe (DR) stimulation on neural activity in the rat lateral hypothalamic area (LHA), including specific glucose-sensitive neurons, were investigated by extracellular and intracellular recording in vivo, and the neurotransmitters involved were determined. 2. In 67 adult male anesthetized rats,...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1988.60.2.524
更新日期:1988-08-01 00:00:00
abstract::Vision in natural situations is different from the paradigms generally used to study vision in the laboratory. In natural vision, stimuli usually appear in a receptive field as the result of saccadic eye movements rather than suddenly flashing into view. The stimuli themselves are rich with meaningful and recognizable...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00733.2011
更新日期:2012-07-01 00:00:00
abstract::We examined the activation kinetics of the delayed rectifier K+ current of bullfrog sympathetic neurons, primarily using whole cell recording. On depolarization, currents activated with a sigmoid delay but did not show a Cole-Moore shift. The time course of activation differed systematically from an exponential raised...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.79.5.2345
更新日期:1998-05-01 00:00:00
abstract::The octopus cells of the posteroventral cochlear nucleus receive inputs from auditory-nerve fibers and form one of the major ascending auditory pathways. They respond to acoustic and electrical stimulation transiently and are believed to carry temporal information in the precise timing of their action potentials. The ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.78.2.872
更新日期:1997-08-01 00:00:00
abstract::Morphine remains widely used in clinical settings due to its potent analgesic properties. However, one of the gravest risks of all opioids is their ability to induce respiratory depression and subsequent brain hypoxia that can lead to coma and death. Due to these life-threatening effects, our goal was to examine the e...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00445.2018
更新日期:2018-11-01 00:00:00
abstract::The ventroposterior region of the thalamus of mongrel cats was searched to locate zones activated by somatic stimuli. By using stimuli that selectively excited Pacinian corpuscles, areas activated by this class of afferent fibers were differentiated from regions activated by other classes of cutaneous mechanoreceptors...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1986.56.6.1475
更新日期:1986-12-01 00:00:00
abstract::A stratified organization of visual, somatic and acoustic representations was observed in the cat SC. Cells of the superficial laminae were exclusively visual. Visual, somatic, and acoustic cells were observed in the intermediate laminae while the deeper laminae were predominantly nonvisual. A detailed examination of ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1976.39.2.401
更新日期:1976-03-01 00:00:00
abstract::We charted postnatal changes in the intrinsic electrophysiological properties and synaptic responses of rat intrinsic cardiac ganglion (ICG) neurons. We developed a whole-mount ganglion preparation of the excised right atrial ganglion plexus. Using intracellular recordings and nerve stimulation we tested the hypothesi...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01220.2005
更新日期:2006-06-01 00:00:00
abstract::A tool or probe often functions as an extension of the hand, transmitting vibrations to the hand to produce a percept of the object contacting the tool or probe. This paper reports the psychophysical results of a combined psychophysical and neurophysiological study of the perception of vibration transmitted through a ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1999.81.4.1548
更新日期:1999-04-01 00:00:00
abstract::The excitatory and inhibitory conductances driving the light-evoked currents (LECs) of cat and ferret ON- and OFF-center X ganglion cells were examined in sliced and isolated retina preparations using center spot stimulation in tetrodotoxin (TTX)-containing Ringer. ON-center X ganglion cells showed an increase in an e...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.80.6.2975
更新日期:1998-12-01 00:00:00
abstract::It has been shown that tetanic stimulation of area 2 of the somatosensory cortex produces long-term potentiation in neurons in area 4gamma, and this has been suggested as the basis of learning new motor skills. The purpose of this study was to further elucidate the characteristics of this projection by the use of evok...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.77.6.3107
更新日期:1997-06-01 00:00:00
abstract::1. It was the aim of this study to show that nucleus Z of the cat medulla acts as a relay between the spinal cord and the ventral lateral (VL) nucleus of the motor thalamus. For this purpose, extracellular recordings were made from neurons that were antidromically identified by stimulation in the rostral thalamus, par...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1993.69.5.1607
更新日期:1993-05-01 00:00:00
abstract::1. The pteropod mollusk Clione limacina swims by rhythmical oscillations of two wings, and its spatial orientation during locomotion is determined by tail movements. The majority of neurons responsible for generation of the wing and tail movements are located in the pedal ganglia. On the other hand, the majority of se...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1995.73.5.1912
更新日期:1995-05-01 00:00:00
abstract::Task-dependent selectivity of movement-related neuronal activity in the primate prefrontal cortex. J. Neurophysiol. 80: 3392-3397, 1998. We studied movement-related neuronal activity in the dorsolateral prefrontal cortex from the perspective of a general role for the prefrontal cortex in controlling motor behavior to ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.80.6.3392
更新日期:1998-12-01 00:00:00
abstract::To explore neural mechanisms how olfactory information is processed in the brain and finally converted into behavior, it would be useful to have isolated whole brains that include both olfactory organs and motor output. In the present study, we identified an in vitro index of odor-evoked behavior in the terrestrial mo...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00247.2003
更新日期:2004-01-01 00:00:00
abstract::1. Less than two segments of the cervical spinal cord of the mudpuppy (Necturus maculatus) is sufficient to generate a locomotor rhythm with application of N-methyl-D-aspartic acid (NMDA). We have recorded intracellularly from rhythmically active interneurons in these segments and classified them according to their ph...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.71.6.2025
更新日期:1994-06-01 00:00:00
abstract::1. The discharge patterns and axonal projections of saccadic long-lead burst neurons (LLBNs) with somata in the pontine reticular formation were studied in alert squirrel monkeys with the use of the method of intraaxonal recording and horseradish peroxidase injection. 2. The largest population of stained neurons were ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.76.1.353
更新日期:1996-07-01 00:00:00
abstract::We constantly make eye movements to bring objects of interest onto the fovea for more detailed processing. Activity in area V4, a prestriate visual area, is enhanced at the location corresponding to the target of an eye movement. However, the precise role of activity in V4 in relation to these saccades and the modulat...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00898.2009
更新日期:2010-10-01 00:00:00
abstract::1. In order to investigate the effects of an imbalance in stimulation to the eyes without the confounding influence of continuous deprivation of one eye, we reared cats with unequal alternating monocular exposure (AME) and, for comparison, cats with equal AME. We recorded extracellularly from single cells in area 17 o...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1983.49.3.804
更新日期:1983-03-01 00:00:00
abstract::The suppressive action of a moving textured background on responses to moving bars was investigated in 118 striate neurons, 19 dorsal lateral geniculate neurons, and 5 perigeniculate neurons in paralyzed and anesthetized cats. In standard conditions the background was a two-dimensional (2D) noise pattern, the bar move...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1987.57.6.1767
更新日期:1987-06-01 00:00:00