Abstract:
:The diagnosis of melanoma metastatic to lymph node remains a difficult problem given its histological diversity. We examined the staining patterns of S-100, NK1/C3, HMB-45, and MART-1 (DC10) in melanoma metastases to lymph nodes. Immunohistochemical stains were performed on tissue sections of 126 formalin-fixed lymph nodes from 126 patients with an established diagnosis of metastatic melanoma. A total of 98% of cases (123 of 126) stained positive for S-100, 93% (117 of 125) stained positive for NK1/C3, 82% (103 of 126) stained positive for MART-1, and 76% (95 of 125) stained positive for HMB-45. The distribution and intensity of staining varied among these markers. A diffuse staining pattern, defined as >50% of tumor cells stained, was observed in 83% of MART-1-positive cases but in only 56% of S-100-positive cases, 48% of NK1/C3-positive cases, and 34% of HMB-45-positive cases. A maximally intense signal was almost always observed for MART-1 (83% of positive cases) but was rarely observed for NK1/C3 (20%). S-100 and HMB-45 showed maximally intense staining in 50% and 54% of cases, respectively. S-100 and NK1/C3 stained both histiocytes and melanocytes, whereas MART-1 and HMB-45 stained only melanocytes. Seventy-eight cases (63%) stained positive for all 4 markers, 17 cases (14%) stained for all markers except HMB-45, 13 cases (10%) stained for all markers except MART-1, 6 cases (5%) stained only with S-100 and NK1/C3, 4 cases (3%) stained only with S-100 and HMB-45, and 2 cases stained for all markers except S-100. One case each stained for the following: only S-100, only S-100 and HMB-45, and all markers except NK1/C3. One case exhibited absence of staining for any of these markers. We demonstrate that lymph node metastases of melanoma are heterogeneous with regard to tumor marker expression. S-100 and NK1/C3 were the most sensitive stains for detecting metastatic melanoma; however, they both also stain other nontumor cells in lymph nodes. MART-1 did not stain histiocytes and exhibited a more frequently intense and diffuse staining pattern than NK1/C3. HMB-45 was less sensitive and demonstrated less diffuse staining than MART-1.
journal_name
Hum Patholjournal_title
Human pathologyauthors
Zubovits J,Buzney E,Yu L,Duncan LMdoi
10.1016/j.humpath.2003.09.019subject
Has Abstractpub_date
2004-02-01 00:00:00pages
217-23issue
2eissn
0046-8177issn
1532-8392pii
S0046817703006105journal_volume
35pub_type
杂志文章相关文献
HUMAN PATHOLOGY文献大全abstract::Metallothionein is a group of small molecular weight cysteine-rich proteins with a broad variety of functions. Metallothionein has been shown to regulate apoptosis and proliferation. Overexpression of metallothionein frequently occurs in human tumors and is related to prognosis as well as therapy response. However, me...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2009.01.026
更新日期:2009-12-01 00:00:00
abstract::Human papilloma virus (HPV) has emerged as the key etiologic driver in the majority of cancers at head and neck mucosal sites. First described in the uterine cervix and subsequently in oropharyngeal squamous cell carcinomas, the morphologic and clinical spectrum of HPV-related neoplasia has continued to expand and now...
journal_title:Human pathology
pub_type: 杂志文章,评审
doi:10.1016/j.humpath.2019.09.001
更新日期:2019-12-01 00:00:00
abstract::Ultrastructural studies of the brain in three cases of cerebral amyloid angiopathy demonstrated that the vascular amyloid in this disease is composed of fibrils identical to those in systemic amyloid disease, mixed with a large amount of vesicular debris. Amyloid appears to be derived from circulating protein and is d...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(82)80251-7
更新日期:1982-12-01 00:00:00
abstract::Congenital subclavian steal may result from various anomalies of the aortic arch. The most common predisposing type is the right aortic arch with an isolated left subclavian artery arising from a left ductus arteriosus. A rare variant includes isolation of the left common carotid as well as the left subclavian arterie...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(81)80200-6
更新日期:1981-10-01 00:00:00
abstract::We reviewed open lung biopsies from 35 patients with Wegener's (pathergic) granulomatosis in order to study the histogenesis of the pulmonary lesions and to identify the early lesions. The process of pathergic necrosis is fundamental in the production of extravascular and vascular lesions and was divided into micronec...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(88)80088-1
更新日期:1988-09-01 00:00:00
abstract::Neoglycoproteins are readily available conjugates of a histochemically inert carrier protein and histochemically crucial carbohydrate moieties which are covalently attached to the carrier protein by chemical synthesis. Biotinylation renders these conjugates detectable in formalin-fixed, paraffin-embedded tissue sectio...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(89)90045-2
更新日期:1989-04-01 00:00:00
abstract::We have previously reported that the drs gene has the ability to suppress transformation by v-src and v-K-ras in the rat cell line F2808. We have also shown that the expression of drs mRNA is markedly reduced in a variety of human cancer cell lines, suggesting that down-regulation of drs mRNA is correlated with the de...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(03)00240-5
更新日期:2003-07-01 00:00:00
abstract::A 61-year-old woman was admitted to the hospital because of general fatigue. Laboratory examinations showed hyponatremia, plasma hypo-osmolarity, and inappropriate increased concentration of the plasma antidiuretic hormone (ADH) in the presence of concentrated urine. Magnetic resonance imaging revealed a mass lesion i...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(96)90094-5
更新日期:1996-05-01 00:00:00
abstract::Extratubal secondary trophoblastic implants (ESTI) are a rare complication of conservative laparoscopic procedures for tubal ectopic pregnancies. These implants present with persistent beta-hCG titers postoperatively and are probably the result of disruption of the ectopic pregnancy at salpingostomy or morcellation of...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(98)90231-3
更新日期:1998-02-01 00:00:00
abstract::The National Cancer Institute sponsored a Borderline Ovarian Tumor Workshop held in August 2003 in Bethesda, MD. This report was developed from discussions at the Workshop. The participants acknowledged several areas of disagreement on basic terminology issues and agreed that a glossary with example images would help ...
journal_title:Human pathology
pub_type:
doi:10.1016/j.humpath.2004.03.004
更新日期:2004-08-01 00:00:00
abstract::The canals of Hering (CoH), converging from the hepatic lobule onto the portal tract, connect bile canaliculi to the interlobular bile ducts, and represent the most proximal portion of the bile drainage pathway with a cholangiocyte lining. In this study we sought to ascertain whether this proximal pathway is involved ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1053/hupa.2002.128060
更新日期:2002-10-01 00:00:00
abstract::This article briefly describes the origin of one of the more common (food-related) pathologic terms--"bread and butter pericarditis." The eminent French physician, Laennec, the inventor of the stethoscope amongst several other important medical contributions, can be credited for having coined the term that is still in...
journal_title:Human pathology
pub_type: 传,历史文章,杂志文章
doi:10.1016/j.humpath.2003.08.029
更新日期:2004-02-01 00:00:00
abstract::The authors present 11 cases of plexiform neurofibroma (PN) that featured a very characteristic type of cell appearing as multivacuolated mucin-filled cells (MMFC). The 11 cases were obtained after reviewing 109 cases of PN. Six out of 10 patients showed clinical features of neurofibromatosis type 1. The size of PN ra...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2016.10.010
更新日期:2017-02-01 00:00:00
abstract::We describe the pathology of 5 patients with germline PRKACA copy number gain and Cushing syndrome: 4 males and 1 female, aged 2 to 43 years, including a mother and son. Imaging showed normal or slightly enlarged adrenal glands in 4 patients and a unilateral mass in the fifth. Biochemically, the patients had corticotr...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2014.09.005
更新日期:2015-01-01 00:00:00
abstract::Kaposi sarcoma-associated herpesvirus (KSHV) causes Kaposi sarcoma and is also associated with primary effusion lymphoma, a subset of diffuse large B-cell lymphomas, and multicentric Castleman disease. Because KSHV infection is endemic in sub-Saharan Africa, we sought to identify cases of KSHV-positive non-Hodgkin lym...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2006.08.009
更新日期:2007-02-01 00:00:00
abstract::We introduce a novel hybrid telepathology system providing simultaneous transmission of both real-time microscopy and static imagery at a reasonable cost. It is capable of capturing static, true color images at resolution of 1,520 x 1,144 pixels and providing live transmission of real-time video images of the glass sl...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1053/hupa.2000.9196
更新日期:2000-07-01 00:00:00
abstract::We describe a method for the immunocytochemical assessment of estrogen receptor (ER) status on routinely processed, formalin-fixed tissue using a recently developed commercially available monoclonal antibody (Dako 1D5) with a microwave antigen retrieval technique. A series of 90 cases of human breast carcinoma was ana...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(95)90060-8
更新日期:1995-03-01 00:00:00
abstract::The cell of origin of parafollicular (monocytoid) B cell lymphoma (PBCL), splenic marginal zone lymphoma (SMZL), and hairy cell leukemia (HCL) is controversial. To better understand the relationship between these low-grade B-cell neoplasms, we analyzed the nucleotide sequences of the rearranged immunoglobulin heavy (I...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/s0046-8177(99)90010-2
更新日期:1999-03-01 00:00:00
abstract::Tissue samples from patients with carcinoma of the prostate of various Gleason grades were examined for the frequency of apoptotic bodies. Apoptotic bodies were scored by morphometric methods using hematoxylin-eosin (HE)-stained sections from surgical specimens of prostate cancer. Non-neoplastic prostate tissue adjace...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(94)90249-6
更新日期:1994-08-01 00:00:00
abstract::We report three cases of follicular dendritic cell sarcoma (FDCS) showing a hitherto undescribed histological pattern consisting of nodular tumor growth associated with small B lymphocytes. FDCS tumor cells consistently showed large epithelioid features and were intermingled with small lymphocytes in the nodules in tw...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2011.02.029
更新日期:2012-02-01 00:00:00
abstract::Pancreatic cancer (PaCA) is a deadly disease with few systemic therapeutic options. The head of the pancreas is the most innervated part and most common location of cancer. However, little is known about the contribution of the nerve-cancer interaction to facilitate pancreatic progression. To quantify PaCA axonogenesi...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2016.02.001
更新日期:2016-06-01 00:00:00
abstract::Snail1 and ZEB1 are transcriptional repressors that drive tumor initiation and metastasis in animal models. Snail1 and ZEB1 are frequently coexpressed in tumor cell lines, suggesting that these factors may cooperate to promote tumor progression. However, coexpression of these transcriptional repressors in primary huma...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2010.11.004
更新日期:2011-08-01 00:00:00
abstract::Mantle cell lymphoma (MCL) is typically characterized by t(11;14), which places the IGH@ enhancer elements upstream of CCND1. This fusion results in up-regulation of CCND1 and consequently its protein product cyclin D1. Recent studies have shown that in MCL, mutations or translocations occurring within the 3' untransl...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2017.01.001
更新日期:2017-06-01 00:00:00
abstract::Twenty-five uterine stromal neoplasms (five stromal nodules and 12 low-grade and eight high-grade stromal sarcomas) were studied to determine the correlation between clinicopathologic features, flow cytometric tumor DNA content and proliferative fraction, and patient outcome. Fifteen of the 20 sarcomas (five of them h...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(91)90179-s
更新日期:1991-09-01 00:00:00
abstract::To investigate the expression and clinical significance of long noncoding RNA (lncRNA) in gastric cancer, we applied microarray analysis to obtain expression profiles of protein-coding genes and lncRNAs in tumor and paired adjacent nontumor tissues. We found that 41 lncRNAs were up-regulated and 31 lncRNAs were down-r...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2018.04.008
更新日期:2018-08-01 00:00:00
abstract::Histological criteria for the diagnosis of reflux esophagitis include basal zone hyperplasia, stromal papillae elongation, and inflammatory infiltrate. However, endoscopic esophageal biopsy specimens may include little or no lamina propria. Intraepithelial T lymphocytes, seen in hematoxylin and eosin-stained sections ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2003.07.006
更新日期:2004-01-01 00:00:00
abstract::We studied renal tissues from 203 patients with acquired immunodeficiency syndrome (AIDS). Of the 203 patients, 20 showed light-microscopic changes characteristic of AIDS-associated nephropathy (AAN). Fifteen of the 20 (group A) were examined by immunohistochemistry using Mycoplasma fermentans (incognitus strain)-spec...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/0046-8177(91)90063-u
更新日期:1991-01-01 00:00:00
abstract::PELP1 is a novel coregulator of nuclear hormone receptors and is implicated in playing a role in driving breast cancer and enhancing metastatic potential. The PELP1 protein expression and potential role of PELP1 in triple-negative breast carcinoma (TNBC) have not been well characterized. We investigated PELP1 expressi...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2015.07.023
更新日期:2015-12-01 00:00:00
abstract::Human papillomavirus-related oropharyngeal squamous cell carcinoma has a unique biology and improved prognosis. A new focus is to identify prognostic biomarkers specifically in this human papillomavirus-positive cohort. We analyzed cyclin D1 immunostaining on a tissue microarray of patients with known clinical follow-...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2013.01.021
更新日期:2013-08-01 00:00:00
abstract::Metanephric stromal tumors (MSTs) are rare renal stromal tumors that predominantly affect children. They belong to the metanephric family of tumors, along with metanephric adenofibroma and metanephric adenoma. The previous documentation of BRAF exon 15 mutations in 88% of metanephric adenomas and in isolated cases of ...
journal_title:Human pathology
pub_type: 杂志文章
doi:10.1016/j.humpath.2016.09.025
更新日期:2017-02-01 00:00:00