Ethanol induces volume changes and gap junction closure via intracellular Ca2+ signalling pathway in cultured rabbit gastric epithelial cells.

Abstract:

BACKGROUND:Ethanol is a well-established 'barrier breaker' in gastric mucosa, but its effects at cellular level remain to be detailed. METHODS:Gastric epithelial cells were isolated from rabbits and cultured to monolayers. Intracellular calcium was measured spectrofluorometrically with fura-2. The patency of gap junctions was assessed by photobleaching a small area of 5-carboxyfluorescein loaded monolayer and measuring recovery of fluorescence. For cell volume measurements the change in fluorescence intensity was followed in calcein-loaded monolayers with a confocal microscope. RESULTS:Intracellular calcium concentration was increased from 65 +/- 9 to 140 +/- 17 nM; recovery of fluorescence signal after photobleaching was diminished from 53% +/- 11% to 9% +/- 3%; and cell volume was decreased significantly after 10 min exposure to 5% (vol/vol) ethanol. This volume decrease was prevented with serosal application of the potassium channel blocker, quinine, or by blocking the intracellular calcium signalling pathway with the intracellular calcium-chelating agent BAPTA. This suggests that luminal ethanol opens the basolateral calcium-dependent potassium selective channels via calcium signalling pathway, with resultant shrinkage of the cell. CONCLUSION:Intracellular calcium concentration is increased, gap junctions are closed and cell volume is decreased after exposure to 5% ethanol. Since gap junctions are known to be calcium gated, it is likely that their closure is secondary to the elevated cytosolic calcium in ethanol injured cells. This may have a protective function by limiting intercellular spread of impending cell injury. The opening of the basolateral potassium channel probably underlies the ethanol-induced cell shrinkage and might contribute to the ethanol-provoked epithelial damage.

journal_name

Scand J Gastroenterol

authors

Mustonen H,Kiviluoto T,Puolakkainen P,Kivilaakso E

doi

10.1080/00365520310007855

subject

Has Abstract

pub_date

2004-02-01 00:00:00

pages

104-10

issue

2

eissn

0036-5521

issn

1502-7708

journal_volume

39

pub_type

杂志文章
  • Bile bacterial flora and its in vitro resistance pattern in patients with acute cholangitis resulting from choledocholithiasis.

    abstract:INTRODUCTION:Cholangitis and biliary sepsis are severe infectious diseases, which are often observed in patients with choledocholithiasis. The antimicrobial therapy is effective if started as soon as the diagnosis is made. Therefore, the profile of bile pathogens and its susceptibility to a number of antibiotics were e...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365521.2011.560676

    authors: Karpel E,Madej A,Bułdak Ł,Duława-Bułdak A,Nowakowska-Duława E,Łabuzek K,Haberka M,Stojko R,Okopień B

    更新日期:2011-07-01 00:00:00

  • Pulmonary hydrogen and methane and plasma ammonia after the administration of lactulose or sorbitol.

    abstract::The gut microfloral metabolism of sorbitol and lactulose was investigated in 10 healthy control subjects and 10 patients with cirrhosis of the liver, by measuring the pulmonary excretion of the fermentational gases hydrogen and methane during 3 h. A possible acute effect on venous plasma ammonia after a single dose of...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528309181604

    authors: Beaven J,Bjørneklett A,Jenssen E,Blomhoff JP,Skrede S

    更新日期:1983-05-01 00:00:00

  • Short stricture of the rectum regressing on discontinuation of hormone replacement therapy: endoscopic appearance, multiple biopsies and clinical improvement insufficient for diagnosis.

    abstract::A short, benign-looking stricture of the rectum presented itself clinically as subileus in a middle-aged woman after 5 years on hormone replacement therapy (HRT) and 8 years after curative surgery for cancer of the ovaries. Radiological work-up and multiple, repeated biopsies supported the endoscopically appearing ben...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520510011614

    authors: Hoff G,Wiig J,Reed W,Hole KH,Scheistrøen M,Henriksen H

    更新日期:2005-03-01 00:00:00

  • Anti-CagA and anti-VacA antibodies in Helicobacter pylori-infected patients with and without peptic ulcer disease in Serbia and Montenegro.

    abstract:BACKGROUND:The expression of two Helicobacter pylori proteins, CagA and VacA, is associated with more severe pathogenesis and clinical outcomes of the infection. However, this association varies among geographical regions and ethnic groups. We therefore evaluated CagA and VacA seroprevalence in H. pylori-positive dyspe...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520310008403

    authors: Sokic-Milutinovic A,Wex T,Todorovic V,Milosavljevic T,Malfertheiner P

    更新日期:2004-03-01 00:00:00

  • Adhesion of bacteria to the human small-intestinal mucosa. A scanning electron microscopic study.

    abstract::During the period 1 January 1980 to 30 June 1986, a total of 543 small-intestinal biopsy specimens from adults with gastrointestinal symptoms were available for routine analysis with correlated scanning electron microscopy and light microscopy. Adhesion of microorganisms was found in 77 biopsy specimens. Microorganism...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528909089229

    authors: Hörstedt P,Danielsson A,Nyhlin H,Stenling R,Suhr O

    更新日期:1989-09-01 00:00:00

  • Colonic expression of heme oxygenase-1 is associated with a better long-term survival in patients with colorectal cancer.

    abstract:OBJECTIVE:Heme oxygenase-1 (HO-1) has emerged as a crucial mediator of mucosal defense in the gastrointestinal tract. Its metabolic pathway products, biliverdin/bilirubin and carbon monoxide, can reduce oxidative stress and inflammation, and promote resistance to apoptosis. The role of HO-1 in gastrointestinal malignan...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520701192383

    authors: Becker JC,Fukui H,Imai Y,Sekikawa A,Kimura T,Yamagishi H,Yoshitake N,Pohle T,Domschke W,Fujimori T

    更新日期:2007-07-01 00:00:00

  • Type I and III collagens in human colon cancer and diverticulosis.

    abstract:BACKGROUND:Collagens are major proteins in the extracellular matrix, providing tissues with tensile strength. They are also important for cell adhesion and the invasion of malignant tumours. METHODS:Thirty-nine samples of human colon (24 diverticulosis, 6 malignant tumours, 9 controls) were collected during elective s...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/003655200750023435

    authors: Bode MK,Karttunen TJ,Mäkelä J,Risteli L,Risteli J

    更新日期:2000-07-01 00:00:00

  • Sexuality in patients with ulcerative colitis before and after restorative proctocolectomy: a prospective study.

    abstract:BACKGROUND:The purpose of this study was to assess sexuality in patients with ulcerative colitis (UC), before and one year after an ileal-pouch anal anastomosis (IPAA). METHODS:Group I comprised 14 medically treated patients (7 M) and Group II comprised 29 patients (18 M), operated with colectomy and ileostomy, with p...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520310008449

    authors: Berndtsson I,Oresland T,Hultén L

    更新日期:2004-04-01 00:00:00

  • Cimetidine on-demand in dyspepsia. Experience with randomized controlled single-subject trials.

    abstract::Double-blind randomized controlled trials in single subjects (N of 1 RCTs) have demonstrated a beneficial symptomatic effect of cimetidine in reflux- or ulcer-like non-ulcer dyspepsia (NUD). However, spontaneous fluctuations in symptoms reduce the validity of such trials when performed as continuous trials with fixed ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.3109/00365529208999947

    authors: Johannessen T,Petersen H,Kristensen P,Fosstvedt D,Kleveland PM,Dybdahl J,Løge I

    更新日期:1992-01-01 00:00:00

  • Health-related quality of life after ileal J-pouch-anal anastomosis for ulcerative colitis: long-term results.

    abstract:BACKGROUND:This questionnaire study examined associations between health-related quality of life and long-term follow-up results with J-pouch anal anastomosis for ulcerative colitis. METHODS:A two-part questionnaire was sent to patients treated for ulcerative colitis by handsewn J-pouch-anal anastomosis at our institu...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/003655299750026065

    authors: Tiainen J,Matikainen M

    更新日期:1999-06-01 00:00:00

  • Association of polymorphism of the p22PHOX component of NADPH oxidase in gastroduodenal diseases in Japan.

    abstract:OBJECTIVE:Superoxide has been implicated in the pathogenesis of Helicobacter pylori-related diseases through inflammation. NADPH oxidase, a major source of superoxide generation, plays a critical role in H. pylori-related gastric inflammation. The aim of this study was to clarify the effect of the p22PHOX C242T polymor...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520701702348

    authors: Tahara T,Shibata T,Wang F,Nakamura M,Okubo M,Yoshioka D,Sakata M,Nakano H,Hirata I,Arisawa T

    更新日期:2009-01-01 00:00:00

  • Effects of posture on the physiology of gastric emptying: a magnetic resonance imaging study.

    abstract:OBJECTIVE:Gastric contents empty from the stomach despite frequent changes in body position. The mechanism that maintains gastric emptying independent of position is poorly understood. The aim of this study was to determine the effects of body position on gastric emptying and motor function. MATERIAL AND METHODS:Twelv...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520600610451

    authors: Steingoetter A,Fox M,Treier R,Weishaupt D,Marincek B,Boesiger P,Fried M,Schwizer W

    更新日期:2006-10-01 00:00:00

  • Helicobacter pylori and bleeding duodenal ulcer: prevalence of the infection and role of non-steroidal anti-inflammatory drugs.

    abstract:BACKGROUND:Several authors have reported low prevalence of Helicobacter pylori infection in patients with upper gastrointestinal bleeding (UGIB). Our aim was to study the prevalence of H. pylori in bleeding duodenal ulcer (DU), with both invasive and non-invasive methods, and to assess the role of non-steroidal anti-in...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/003655201300191978

    authors: Gisbert JP,Gonzalez L,de Pedro A,Valbuena M,Prieto B,Llorca I,Briz R,Khorrami S,Garcia-Gravalos R,Pajares JM

    更新日期:2001-07-01 00:00:00

  • Differences in the endoscopic closure of colonic perforation due to diagnostic or therapeutic colonoscopy.

    abstract:OBJECTIVE:To describe the experience of a single center regarding the feasibility of endoscopic closure of iatrogenic colonic perforations and to elucidate differences between the efficacy of endoscopic clip closure due to diagnostic or therapeutic colonoscopy. MATERIAL AND METHODS:A retrospective institutional comput...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365521.2013.793737

    authors: Magdeburg R,Sold M,Post S,Kaehler G

    更新日期:2013-07-01 00:00:00

  • Metronidazole in Crohn's disease. A double blind cross-over clinical trial.

    abstract::Twenty-two patients with active Crohn's disease treated with salazosulfapyridin or prednisone, were in addition given metronidazole 1.000 mg daily or placebo for two months each in a double-blind cross-over study. No statistically significant effect was observed on the overall clinical condition in the 20 patients who...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.3109/00365527809179816

    authors: Blichfeldt P,Blomhoff JP,Myhre E,Gjone E

    更新日期:1978-01-01 00:00:00

  • Effects of somatostatin-14 and somatostatin-28 on plasma hormonal and gastric secretory responses to cephalic and gastrointestinal stimulation in man.

    abstract::This study was designed to determine the influence of cephalic and gastrointestinal meal stimulation on plasma levels of somatostatin-like immunoreactivity (SLI) and to compare plasma hormonal and gastric secretory effects of somatostatin-14 (SS-14) and its putative prohormone, somatostatin-28 (SS-28), in humans. Ceph...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章

    doi:10.3109/00365528509089629

    authors: Konturek SJ,Kwiecień N,Obtułowicz W,Bielański W,Oleksy J,Schally AV

    更新日期:1985-01-01 00:00:00

  • Regional distribution and localization of zinc and metallothionein in the intestine of rats fed diets differing in zinc content.

    abstract:BACKGROUND:Zinc (Zn) is protective and enhances epithelial repair in gut diseases. In this study we investigate the localization and distribution of Zn and its binding protein, metallothionein (MT), in the gut of rats fed diets varying in Zn content. METHODS:Male-Sprague Dawley rats were fed low, normal, high, or exce...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/003655299750025895

    authors: Tran CD,Butler RN,Howarth GS,Philcox JC,Rofe AM,Coyle P

    更新日期:1999-07-01 00:00:00

  • Faecal calprotectin shedding after short-term treatment with non-steroidal anti-inflammatory drugs.

    abstract:BACKGROUND:Increased faecal calprotectin shedding indicates gastrointestinal mucosal inflammation. METHODS:We studied the effect of short-term treatment with non-steroidal anti-inflammatory drugs (NSAIDs) on faecal calprotectin shedding in two randomized crossover studies, with treatment regimens of indomethacin or na...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.3109/00365529609006407

    authors: Meling TR,Aabakken L,Røseth A,Osnes M

    更新日期:1996-04-01 00:00:00

  • A case of adenocarcinoma of the small intestine in a Japanese patient with Crohn disease: a report with immunohistochemical and oncogenic analyses.

    abstract::We report a rare case of Crohn disease accompanied by a small-bowel carcinoma that developed in a 54-year-old Japanese man. The ulcerating tumor, which histologically proved to be a poorly differentiated adenocarcinoma and dysplasia surrounding the carcinoma, was located in the diseased ileum. The Ki-67 immunoreactive...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/003655299750024995

    authors: Uesugi H,Mitomi H,Sada M,Takahashi H,Kobayashi K,Igarashi M,Katsumata T,Ihara A,Ohtani Y,Ikeda S,Okayasu I

    更新日期:1999-11-01 00:00:00

  • Surgical outcome evaluation of perforated gastric cancer: from the aspects of both acute care surgery and surgical oncology.

    abstract:BACKGROUND:Perforated gastric cancer (PGC) is a rare condition of gastric cancer (GC). In this study, we sought to assess the outcome of PGC from the aspects of both acute care surgery and surgical oncology at a single institute, Chang Gung Memorial Hospital (CGMH). METHODS:From 1997 to 2013, 6864 patients were diagno...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365521.2017.1369562

    authors: Wang SY,Hsu CH,Liao CH,Fu CY,Ouyang CH,Cheng CT,Hsu JT,Yeh TS,Yeh CN

    更新日期:2017-12-01 00:00:00

  • A characterization in childhood inflammatory bowel disease, a new population-based inception cohort from South-Eastern Norway, 2005-07, showing increased incidence in Crohn's disease.

    abstract:OBJECTIVE:Owing to rising incidence rates in inflammatory bowel disease (IBD), there has been increased interest in causal relationships in pediatric disease. The present population-based inception cohort was recruited in the Oslo area from 2005 to 2007, with the aim of conducting a detailed characterization of treatme...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/00365520802647434

    authors: Perminow G,Brackmann S,Lyckander LG,Franke A,Borthne A,Rydning A,Aamodt G,Schreiber S,Vatn MH,IBSEN-II Group.

    更新日期:2009-01-01 00:00:00

  • Modulation of the adrenergic system in the treatment of postoperative bowel atonia.

    abstract::Sympathetic hyperactivity is considered to be the most important factor in the development of postoperative bowel atonia. In a double-blind study we evaluated the gut motor effects of dihydroergotamine, which predominantly acts as a sympatholytic agent in the gastrointestinal tract. Forty-six patients undergoing chole...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Altaparmakov I,Erckenbrecht JF,Wienbeck M

    更新日期:1984-11-01 00:00:00

  • Ultrasound measurements of calcaneus for estimation of skeletal status in patients with inflammatory bowel disease.

    abstract:BACKGROUND:Patients with inflammatory bowel disease (IBD) are at risk of developing metabolic bone disease. In diagnosing osteoporosis, bone mineral density (BMD) measurements play a key role. Our aims in this study were to assess the skeletal status with quantitative ultrasound (QUS) and to evaluate the ability of thi...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/003655299750025723

    authors: Jahnsen J,Falch JA,Mowinckel P,Aadland E

    更新日期:1999-08-01 00:00:00

  • The impact of atopic disease on the risk of post-infectious fatigue and irritable bowel syndrome 3 years after Giardia infection. A historic cohort study.

    abstract:OBJECTIVE:To investigate whether atopic disease influences the prevalence of irritable bowel syndrome (IBS) and chronic fatigue (CF) after giardiasis. METHODS:A questionnaire was sent to all confirmed cases of giardiasis after a Norwegian outbreak, with response rate of 65.3% (817/1252). Controls were randomly selecte...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365521.2012.696681

    authors: Hunskar GS,Langeland N,Wensaas KA,Hanevik K,Eide GE,Mørch K,Rortveit G

    更新日期:2012-09-01 00:00:00

  • Effect of glucagon-like peptide-1 on gastric somatostatin and gastrin secretion in the rat.

    abstract::The effect of glucagon-like peptide-1 (GLP-1) amide on gastric somatostatin and gastrin secretion was investigated in the isolated, vascularly perfused rat stomach preparation. GLP-1 (7-36) amide, 10(-12) to 10(-7)M, dose-dependently increased gastric somatostatin release, achieving maximal stimulation (314 +/- 15% ab...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529009095514

    authors: Eissele R,Koop H,Arnold R

    更新日期:1990-05-01 00:00:00

  • Effect of pentagastrin on regional gastric blood flow in conscious cats.

    abstract::Total gastric flow studies indicated that a transient, considerable flow increase was followed by a more moderate increase of total gastric flow during pentagastrin stimulation in anesthetized cats. In conscious cats regional gastric flow studies, using hydrogen clearance techniques, suggested that raised flow to the ...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365528209182080

    authors: Semb BK

    更新日期:1982-04-01 00:00:00

  • Prolonged remission of oesophagitis does not alter the magnitude of oesophageal acid exposure.

    abstract::In a previous study we reported lack of improvement in oesophageal motor function after complete healing of oesophagitis achieved by treatment with omeprazole for a median duration of 12 weeks. This study investigates the effect on oesophageal acid exposure of a longer period of complete remission. It was decided to a...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.3109/00365529409090430

    authors: Singh P,Taylor RH,Colin-Jones DG

    更新日期:1994-01-01 00:00:00

  • Effect of duodenal feeding on serum gastrin and acid response to pentagastrin in cats.

    abstract::Five cats were provided with a gastric fistula (GF) and a Heidenhain pouch (HP), and the proximal small intestine was excluded and provided with a duodenal fistula. A protein-rich meal, introduced into the duodenum (duodenal feeding) caused no serum gastrin increase, which supports our previous conclusion that duodena...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:

    authors: Fernström M,Nilsson G,Emås S

    更新日期:1984-01-01 00:00:00

  • Quality of life study in a regional group of patients with Crohn disease. A structured interview study.

    abstract:BACKGROUND:The course and prognosis of Crohn disease has previously been described in a regional group of patients in Copenhagen County. The aim of the present study was to reveal the quality of life. as judged by the patients, and compared to age- and sex-matched healthy controls. METHODS:Out of 100 consecutive out-p...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 杂志文章

    doi:10.1080/003655200451199

    authors: Guassora AD,Kruuse C,Thomsen OO,Binder V

    更新日期:2000-10-01 00:00:00

  • Fish oil reduces gastric acid secretion.

    abstract:BACKGROUND:The aim of the present investigation was to study gastric acid secretion and release of gastrin, cholecystokinin (CCK), and secretin during intraduodenal perfusion of either fish oil or trioleate. METHODS:Seven healthy volunteers were stimulated on two separate days in random order with intraduodenal perfus...

    journal_title:Scandinavian journal of gastroenterology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1080/003655299750025282

    authors: Riber C,Wøjdemann M,Bisgaard T,Ingels H,Rehfeld JF,Olsen O

    更新日期:1999-09-01 00:00:00