Abstract：
:The present study concerns an ELISA for glial fibrillary acidic protein (GFAP) in cerebrospinal fluid (CSF). The application of the method in CSF of children has previously been described in this journal. We have now adapted the technique to allow determination of the much higher GFAP concentrations found in normal and pathological CSF of adults. The assay range was extended to 16,000 pg/ml. CSF levels as high as 170,000 pg/ml could be assayed since dilution experiments indicated immunological identity between purified GFAP and GFAP in CSF. In normal controls the concentrations correlated significantly with age (P < 0.001, Spearman rank correlation test). The concentrations were less than 200 pg/ml before 20 years of age and increased to between 500 and 1300 pg/ml at approximately 75 years of age. This increase of CSF GFAP probably reflects formation of astroglial filaments in the CNS and it must be taken into consideration when determining the pathological CSF GFAP level. The method was validated using CSF samples from patients with either astrogliosis or acute tissue destruction in the CNS. Although augmented levels were observed in both groups it is quite clear that patients with acute tissue destruction may display very high CSF GFAP concentrations, whereas levels in patients with astrogliosis were only modestly increased if at all.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Rosengren LE,Wikkelsø C,Hagberg Ldoi
10.1016/0165-0270(94)90011-6subject
Has Abstractpub_date
1994-03-01 00:00:00pages
197-204issue
2eissn
0165-0270issn
1872-678Xpii
0165-0270(94)90011-6journal_volume
51pub_type
杂志文章abstract：:The image-combining computer microscope is a new configuration of light microscope and computer graphics instrumentation which provides remarkable morphometric capabilities over a wide range of applications. The key component is the well known camera lucida, an image-combining device that is used to superimpose the co...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(83)90048-1
更新日期：1983-05-01 00:00:00
abstract：:Functional magnetic resonance imaging allows precise localization of brain regions specialized for different perceptual and higher cognitive functions. However, targeting these deep brain structures for electrophysiology still remains a challenging task. Here, we propose a novel framework for MRI-stereotactic registra...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.11.031
更新日期：2012-03-15 00:00:00
abstract：BACKGROUND:Assessing markers of neural activity in rodent behavioural tasks benefits from meaningful comparison with matched control conditions. The Morris water maze is a widely used learning and memory task, but currently implemented control conditions are not optimal. NEW METHOD:We created a novel control condition...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.02.015
更新日期：2019-04-15 00:00:00
abstract：:The goal of the present study was an improvement of the widely used photoconversion technique, which still represents the major approach to the ultrastructural analysis of tissue labelled with fluorescent dyes. Since free access of oxygen to the tissue is essential for the dye-dependent photooxidation of diaminobenzid...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(96)00150-1
更新日期：1997-02-01 00:00:00
abstract：:We have attempted to demonstrate the technical requirements and performance of a microscopical assay using a densitometric application of image analysis to measure immunohistochemical stain intensity. Not surprisingly, the techniques required were more demanding than those used for the quantification of field and obje...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90048-i
更新日期：1992-10-01 00:00:00
abstract：:Although scalp potential distributions do not uniquely determine the location and configuration of neural generators, they are important because they provide the necessary conditions that any hypothesized sources must satisfy and suggest a basis for testing alternate source hypotheses. One problem that could confound ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(96)00060-x
更新日期：1996-10-01 00:00:00
abstract：:A method for automatically analyzing cortical signals recorded with voltage-sensitive dyes and a photodiode array is described. First, a forward-backward non-linear filtering technique is used to eliminate the background noise and preserve the fast transients of the signals. Then the filtered signals are deconvoluted ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(95)00105-0
更新日期：1995-12-01 00:00:00
abstract：:A solution is described for the acquisition on a personal computer of standard pulses derived from neuronal discharge, measurement of neuronal discharge times, real-time control of stimulus delivery based on specified inter-pulse interval conditions in the neuronal spike train, and on-line display and analysis of the ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(95)00061-5
更新日期：1995-11-01 00:00:00
abstract：:A method for selecting a usable carbon-fiber electrode using the equivalent resistance and capacitance is presented. This method uses an instrument with a PC-based look-up table for measuring the electrical characteristics of a carbon-fiber electrode in pulse voltammetry. Using this instrument, the equivalent resistan...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90036-q
更新日期：1993-12-01 00:00:00
abstract：BACKGROUND:Stroke is the third leading cause of death and the leading cause of long-term disability in North America. On average, someone in the US has a stroke every 45 s, and worldwide, stroke claims 15 million lives each year. Therefore, reliable stroke models are vital to the production of effective new therapies f...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.04.008
更新日期：2015-07-15 00:00:00
abstract：BACKGROUND:Application of functional Near InfraRed Spectroscopy (fNIRS) in neurology is still limited as a good optical coupling and optimized optode coverage of specific brain regions remains challenging, notably for prolonged monitoring. METHODS:We propose to evaluate a new procedure allowing accurate investigation ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.08.006
更新日期：2018-11-01 00:00:00
abstract：:A method is described for the isolation of neurons from defined regions of the mammalian central nervous system, by a combination of mechanical and enzymatic means. The procedure liberates neurons free of cellular debris and glial investments, allowing the formation of giga-ohm seals with patch clamp electrodes. The c...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90040-3
更新日期：1986-05-01 00:00:00
abstract：:A semi-automated analysis system based on video image analysis was developed to count labelled and unlabelled nuclei of Schwann cells which had been exposed to tritiated thymidine followed by processing for autoradiography. A Model 3000 Image Analysis system (Image Technology Corporation, Deer Park, NY) was used to ac...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90062-8
更新日期：1987-08-01 00:00:00
abstract：:A method is described enabling the recording of synaptic currents from an isolated interganglionic interneurone in the central nervous system of the cockroach, Periplaneta americana, using an oil-gap recording system. The circuitry contains a Wheatstone bridge in which the preparation is the unknown resistance. Using ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90146-x
更新日期：1988-09-01 00:00:00
abstract：:Effective therapeutic interventions for injuries of the central nervous system such as spinal cord injury are still unavailable, having a great impact on the quality of life of victims and their families, as well as high costs in medical care. Animal models of spinal cord injury are costly, time-consuming and labor-in...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.08.024
更新日期：2011-10-15 00:00:00
abstract：:A method for the reconstruction of the individual spike trains from extracellular multineuron recordings is described. A neural network emulation program is trained to recognize a sample set of digitized spikes. The digitized spikes are fed into the neural network, and the network output is used to classify spikes in ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(90)90125-y
更新日期：1990-12-01 00:00:00
abstract：:Recombinant proteins are routinely expressed in heterologous expression systems such as human embryonic kidney 293 (HEK 293) cells. The efficiency of the expression is critical when the expressed protein must be characterized at the single-cell level. Here we describe a simple method by which the protein expression ef...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.09.009
更新日期：2005-03-15 00:00:00
abstract：:Mitochondrial defects have been implicated in the degeneration of axons in a number of CNS disorders, including multiple sclerosis. Uniquely, mitochondria harbor the only non-nuclear DNA (mitochondrial DNA or mtDNA), which encodes functionally important subunits of the respiratory chain. The pattern of mitochondrial r...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.07.022
更新日期：2010-09-30 00:00:00
abstract：:The Barnes maze is a visuo-spatial learning and memory test originally designed for use with rats, and later adapted for use with mice. The Barnes maze design and test procedure vary across studies using mice, but the effects of variation in Barnes maze design and test procedure on learning and memory in mice have not...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.09.027
更新日期：2012-01-30 00:00:00
abstract：BACKGROUND:It has previously been reported that EEG sigma (10-15 Hz) activity during sleep exhibits infraslow oscillations (ISO) with a period of 50 s. However, a detailed analysis of the ISO of individually identified sleep spindles is not available. NEW METHOD:We investigated basic properties of ISO during baseline ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.12.002
更新日期：2019-03-15 00:00:00
abstract：:The immunotoxin 192 IgG-saporin has a high degree of selectivity for cholinergic neurons within the basal forebrain (CBF). Intracerebroventricular delivery of 192 IgG-saporin results in a diffuse and massive depletion of choline acetyltransferase (ChAT) activity in projections of the CBF, and non-selective loss of Pur...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00057-6
更新日期：1999-09-15 00:00:00
abstract：:Hippocampal slices were obtained from hippocampi of patients with temporal lobe epilepsy or from patients with mass lesions located in the temporal lobe. Hippocampal slices were kept alive in a slice chamber and the anterograde tracer neurobiotin was iontophoretically injected into the granule cell layer of the dentat...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00187-5
更新日期：2002-10-15 00:00:00
abstract：:Single, i.e. unconnected spinal or cerebral neurons from chick embryos rapidly regenerate morphologically identifiable axons and dendrites when cultured in a three-dimensional (3-D) extracellular matrix (ECM) consisting of a hydrated native collagen lattice. We now show that it is possible to study the intrinsic elect...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90052-5
更新日期：1987-07-01 00:00:00
abstract：:The EPC-9 patch-clamp amplifier is a digitally controlled analog device for recording currents in membrane patches and in small cells. It has neither front-panel controls nor internal trim adjustments; instead all gain, range-changing, transient cancellation, calibration and other functions are computer controlled. No...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
abstract：BACKGROUND:Fear conditioning (FC) in rodents is the most used animal model to investigate the neurobiology of posttraumatic stress disorder (PTSD). Although research using FC has generated a better understanding of fear memories, studies often rely on mild or moderate FC training and behavioral analysis generally focus...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.02.004
更新日期：2018-03-15 00:00:00
abstract：:A variety of materials has been applied to neuronal cell culture substrates to improve the efficiency of the culture and to provide pertinent cell growth environment. Here we report the application of Parafilm(®) M ('Parafilm') as a novel substrate for neuronal culture and patterning. Cell culture results show that el...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.10.023
更新日期：2012-02-15 00:00:00
abstract：BACKGROUND:Cytokines are key signaling molecules within the immune system that regulate a host's response to pathogens and neuronal damage. Aberrant cytokine signaling has been implicated in many neurological diseases. Therefore, accurately measuring cytokine concentrations within the brain is crucial. NEW METHOD:This...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108601
更新日期：2020-01-22 00:00:00
abstract：:A new data model allowing the coding, graphic representation and metric analysis of dendritic and axonal processes including spines and varicosities, is here described. The model is implemented in an interactive light microscope-computer system and stores the three-dimensional coordinates of the selected neuronal poin...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90022-r
更新日期：1991-03-01 00:00:00
abstract：:We investigated whether conscious rats exhibit hyperalgesia to noxious mechanical stimuli during tail reperfusion following transient tail ischaemia. Mechanical stimuli were delivered by three mechanical algometers, differing in contact area, geometry and rate of application. Tail ischaemia was induced by applying a p...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00313-7
更新日期：2000-11-30 00:00:00
abstract：BACKGROUND:Saccades are rapid eye movements used to gather information about a scene which requires both action and perception. These are usually studied separately, so that how perception influences action is not well understood. In a dual task, where the subject looks at a target and reports a decision, subtle change...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.05.010
更新日期：2014-07-30 00:00:00
