Abstract:
:Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endangered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hirus respectively we found that mitochondria of donor cells can co-exist with recipients in 1-cell and 2-cell stages of the reconstructed embryos but not in the following developmental stages.
journal_name
Acta Biochim Biophys Sin (Shanghai)journal_title
Acta biochimica et biophysica Sinicaauthors
Jiang Y,Liu SZ,Zhang YL,Jiang MX,Sun QY,Chen DYdoi
10.1093/abbs/36.5.371subject
Has Abstractpub_date
2004-05-01 00:00:00pages
371-4issue
5eissn
1672-9145issn
1745-7270journal_volume
36pub_type
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journal_title:Acta biochimica et biophysica Sinica
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journal_title:Acta biochimica et biophysica Sinica
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journal_title:Acta biochimica et biophysica Sinica
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journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1093/abbs/gmq072
更新日期:2010-09-01 00:00:00
abstract::OsEBP-89 gene encodes an ethylene responsive element binding protein (EREBP) transcription factor from rice (Oryza sativa). Northern blot analysis revealed that OsEBP-89 was expressed in root, stem, seeds, flowers and leaves of rice. Histochemical assay showed that GUS expressed mainly in phloem of vascular tissues of...
journal_title:Acta biochimica et biophysica Sinica
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journal_title:Acta biochimica et biophysica Sinica
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abstract::The aim of this study was to investigate the pro-apoptotic mechanism of C-phycocyanin (C-PC)-mediated photodynamic therapy (PDT) in a murine tumor model and cultured MCF-7 cells. The mice were divided into four groups: control, He-Ne laser radiation, C-PC treatment, and C-PC treatment + He-Ne laser radiation. The effe...
journal_title:Acta biochimica et biophysica Sinica
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journal_title:Acta biochimica et biophysica Sinica
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abstract::Rabies virus (RV) causes a fatal disease in both human and animals. The disease can be prevented by post-exposure prophylaxis in individuals exposed to RV. However, the neutralization effect is limited after the virus enters into the host cells. So, it is important to identify new targets for rabies therapy. In this s...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
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abstract::In this study, we identified five miRNAs highly expressed in the small-cell lung cancer (SCLC) cell line NCI-H209. Among them, the expression levels of miR-375 were dramatically elevated in all SCLC cell lines examined, coincident with the expression of the transcription factor achaete-scute complex homolog 1 (ASCL1)....
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
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更新日期:2012-02-01 00:00:00
abstract::Circulating endothelial progenitor cells (cEPCs) play an important role in cancer development. Previous studies showed that serum carcinoembryonic antigen (CEA) levels and the number of circulating endothelial progenitor cells (cEPCs) in the peripheral blood are both involved in tumor neoangiogenesis, and can be used ...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
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更新日期:2018-03-01 00:00:00
abstract::Mitochondria play a key role in the regulation of apoptosis induced by numerous antitumor chemotherapeutic and other toxic agents. Photodynamic therapy (PDT) exerts significant cellular killing efficacy through either an apoptotic or necrotic cell death pathway. This study investigated the mechanism underlying the kil...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1111/j.1745-7270.2005.00067.x
更新日期:2005-07-01 00:00:00
abstract::In the present study, paclitaxel (PTX) were encapsulated with polyethylene glycol (PEG)-polylactide (PLA)/D-α tocopheryl polyethylene glycol 1000 succinate (TPGS) (PEG-PLA/TPGS) and the enhanced anti-tumor activity of this PTX mixed micelles (PTX-MM) was evaluated in lung cancer cells. The PTX-MM prepared by a solvent...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1093/abbs/gmv110
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journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1093/abbs/gmz144
更新日期:2020-02-03 00:00:00
abstract::Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been reported to interact with proteins containing the polyglutamine (polyQ) domain. The present study was undertaken to evaluate the potential contributions of the polyQ and polyproline (polyP) domains to the co-localization of mutant huntingtin (htt) and GAPDH. Ov...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1111/j.1745-7270.2007.00352.x
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abstract::Some barley mutants can synthesize neither anthocyanins nor proanthocyanidins in the seed coat, which is related to several genes in locus Ant13, but the exact model of action remains unknown. We used the cDNA microarray technology with barley transcription-deficient mutant (ant13-152) that does not synthesize proanth...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1093/abbs/36.10.695
更新日期:2004-10-01 00:00:00
abstract::Emodin, a natural anthraquinone extracted from the Chinese herbs rhubarb and giant knotweed rhizome, has been reported to enhance osteoblast differentiation. However, the mechanisms underlying its ability to regulate osteogenesis are unclear. The objective of this study was to determine the role of emodin in osteoblas...
journal_title:Acta biochimica et biophysica Sinica
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journal_title:Acta biochimica et biophysica Sinica
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abstract::Mutant N-terminal huntingtin (Htt) protein resulting from Huntington's disease (HD) with expanded polyglutamine accumulates and forms aggregates in vulnerable neurons. Both ubiquitin proteasomal and autophagic pathways contribute to the degradation of mutant Htt. Here, we focus on the involvement of chaperone-mediated...
journal_title:Acta biochimica et biophysica Sinica
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abstract::Heart failure (HF) induced by ischemia myocardial infarction (MI) is one of the major causes of morbidity and mortality all around the world. Atorvastatin, a hydroxymethylglutaryl coenzyme A reductase inhibitor, has been demonstrated to benefit patients with ischemic or non-ischemic-induced HF, but the mechanism is st...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
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更新日期:2018-12-01 00:00:00
abstract::MicroRNAs (miRNAs) function as key regulators of gene expression in various cancers. In this study, the aim is to explore the roles and regulation mechanism of miR-181c in neuroblastoma (NB) cells. We found that miR-181c was downregulated in metastatic NB tissues, compared with primary NB tissues. Then functional stud...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1093/abbs/gmt124
更新日期:2014-01-01 00:00:00
abstract::hPFTAIRE1 is a Cdc2-related kinase family member. To search its substrates and regulatory proteins, hPFTAIRE1 was fused to LexA and used as a bait to screen a human brain LexA two-hybrid library. In this screening, seven hPFTAIRE1 interacting proteins, including promyelocytic leukemia zinc finger (PLZF), were obtained...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1111/j.1745-7270.2006.00145.x
更新日期:2006-03-01 00:00:00
abstract::Posttranslational modifications (PTMs) of histone proteins, such as acetylation, methylation, phosphorylation, and ubiquitylation, play essential roles in regulating chromatin dynamics. Combinations of different modifications on the histone proteins, termed 'histone code' in many cases, extend the information potentia...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章,评审
doi:10.1093/abbs/gmr100
更新日期:2012-01-01 00:00:00
abstract::Although viral vectors are efficient systems to transfer foreign genes into cells or target tissues, safety issues remain in relation to human gene therapy. Microbubbles currently used as ultrasound contrast agents have been applied in transfection of genes. This study was designed to test the transfection efficiency ...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1093/abbs/36.12.824
更新日期:2004-12-01 00:00:00
abstract::Anoikis resistance is a critical process for cancer cell metastasis in non-small cell lung cancer (NSCLC), and microRNA-1827 (miR-1827) is closely correlated with NSCLC metastasis. In this study, we aimed to evaluate the role of miR-1827 in regulating the anoikis resistance of NSCLC. The results showed that miR-1827 l...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1093/abbs/gmaa102
更新日期:2020-10-19 00:00:00
abstract::In the present study, we investigated the inhibition of the Lux quorum-sensing system by N-acyl cyclopentylamine (Cn-CPA). The Lux quorum-sensing system regulates luminescence gene expression in Vibrio fischeri. We have already reported on the synthesis of Cn-CPA and their abilities as inhibitors of the quorum-sensing...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1111/j.1745-7270.2008.00490.x
更新日期:2008-12-01 00:00:00
abstract::We previously identified a matrix protein, MSI7, from pearl oyster Pinctada fucata. According to the structural analysis, the DGD site in the N-terminal of MSI7 is crucial for its role in the shell formation. In this study, we expressed a series of recombinant MSI7 proteins, including the wild-type and several mutants...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1093/abbs/gmp086
更新日期:2009-11-01 00:00:00
abstract::Bcl-2, a prominent member of the Bcl-2 family proteins, is responsible for the dysregulation of apoptosis and resistance to chemotherapy. In this study, we investigated whether small hairpin RNA (shRNA) targeting at Bcl-2 mRNA could enhance cytarabine (Ara-C)-induced apoptosis in Raji cells. Bcl-2 shRNA was transfecte...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1093/abbs/gmn015
更新日期:2009-02-01 00:00:00
abstract::An L-amino acid oxidase (LAAO), NA-LAAO, was purified from the venom of Naja atra. Its N-terminal sequence shows great similarity with LAAOs from other snake venoms. NA-LAAO dose-dependently induced aggregation of washed human platelets. However, it had no activity on platelets in platelet-rich plasma. A low concentra...
journal_title:Acta biochimica et biophysica Sinica
pub_type: 杂志文章
doi:10.1111/j.1745-7270.2008.00372.x
更新日期:2008-01-01 00:00:00