Analysis of the interaction between hPFTAIRE1 and PLZF in a yeast two-hybrid system.

abstract：:Sequence analysis of oxazolomycin (OZM) biosynthetic gene cluster from Streptomyces albus JA3453 revealed a gene, ozmH, encoding a hybrid polyketide and non-ribosomal peptide enzyme. Tandem ketosynthase (KS) domains (KS 10-1 and KS 10-2) were characterized and they show significant homology with known KSs. Using an al...

journal_title：Acta biochimica et biophysica Sinica

authors： Song D,Coughlin J,Ju J,Zhou X,Shen B,Zhao C,Deng Z

更新日期：2008-04-01 00:00:00

abstract：:To investigate whether apelin-13 induced THP-1 monocytes (MCs) adhesion to ECV304 human umbilical vein endothelial cells (HUVECs) via 14-3-3 signaling transduction pathway and the potential novel physiological function and signaling transduction pathway of apelin-APJ, HUVECs ECV304 were cultured in DMEM and MCs THP-1 ...

journal_title：Acta biochimica et biophysica Sinica

authors： Li X,Zhang X,Li F,Chen L,Li L,Qin X,Gao J,Su T,Zeng Y,Liao D

更新日期：2010-06-15 00:00:00

abstract：:Mediator complex subunit 1 (Med1)/Thyroid hormone receptor-associated protein 220 (TRAP220), an essential component of thyroid hormone receptor-associated proteins (TRAP)/mediator, plays important roles in hormone responses and tumorigenesis. However, the role of Med1 in the DNA damage response has not been studied. I...

journal_title：Acta biochimica et biophysica Sinica

authors： Kim HJ,Yun J

更新日期：2017-06-01 00:00:00

abstract：:Hyperglycemia-mediated reactive oxygen species (ROS) accumulation plays an important role in hyperglycemia-induced endothelial injury. Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway inhibition participates in hyperglycemia-induc...

journal_title：Acta biochimica et biophysica Sinica

authors： Chen X,Qi J,Wu Q,Jiang H,Wang J,Chen W,Mao A,Zhu M

更新日期：2020-05-26 00:00:00

abstract：:The recombinant peptide, DBAYL, a promising therapeutic peptide for type 2 diabetes, is a new, potent, and highly selective agonist for VPAC2 generated through site-directed mutagenesis based on sequence alignments of pituitary adenylate cyclase-activating peptide (PACAP), vasoactive intestinal peptide (VIP), and rela...

journal_title：Acta biochimica et biophysica Sinica

authors： Ma Y,Luo T,Xu W,Ye Z,Hong A

更新日期：2012-11-01 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is a fascinating target for cancer therapy due to its significant role in the crossroad of multiple signaling pathways associated with cell proliferation and regulation. Hsp90 inhibitors have the potential to be developed into anti-cancer drugs. Here, we identified nicotinic-mycoepoxydien...

journal_title：Acta biochimica et biophysica Sinica

authors： Sun Y,Xiao S,Chen J,Wang M,Zheng Z,Song S,Zhang L

更新日期：2015-06-01 00:00:00

abstract：:The glutathione S-transferase mu 2 gene (GSTM2) encodes a GST functioning in the elimination of electrophilic compounds and the regulation of cell growth. In this study, the sequence of porcine GSTM2 gene that contains the complete sequence encoding a protein of 218 amino acids was cloned. The deduced amino acid seque...

journal_title：Acta biochimica et biophysica Sinica

authors： Huang J,Xiong Y,Deng C,Zuo B,Xu D,Lei M,Jiang S

更新日期：2007-08-01 00:00:00

abstract：:Reactive oxygen species (ROS), including H(2)O(2), play a critical role in the pathophysiology of osteoporosis. Therefore, agents or antioxidants that can inhibit ROS production have a high clinical value in the treatment of osteoporosis. Curculigoside (CUR), one of the main bioactive phenolic compounds isolated from ...

journal_title：Acta biochimica et biophysica Sinica

authors： Wang Y,Zhao L,Wang Y,Xu J,Nie Y,Guo Y,Tong Y,Qin L,Zhang Q

更新日期：2012-05-01 00:00:00

abstract：:The natural alkaloid berberine has been recently described as a promising anticancer drug. In order to improve its efficacy and bioavailability, several derivatives have been designed and synthesized and found to be even more potent than the lead compound. Among the series of berberine derivatives we have produced, fi...

journal_title：Acta biochimica et biophysica Sinica

authors： Guamán Ortiz LM,Croce AL,Aredia F,Sapienza S,Fiorillo G,Syeda TM,Buzzetti F,Lombardi P,Scovassi AI

更新日期：2015-10-01 00:00:00

abstract：:Several discriminate functions for predicting core promoters that based on the potential cooperation between transcription factor binding sites (TFBSs) are discussed. It is demonstrated that the promoter predicting accuracy is improved when the cooperation among TFBSs is taken into consideration. The core promoter reg...

journal_title：Acta biochimica et biophysica Sinica

authors： Ma XT,Qian MP,Tang HX

更新日期：2004-04-01 00:00:00

abstract：:Receptor of human interleukin 4 (hIL4R) has been found to be present on many types of cancer, so it may be a good target for cancer therapy. Here, fusion toxin gene DT4H has been constructed by fusing DNA sequence encoding the first 389 amino acids of diphtherial toxin (DT), which can not bind its own receptor, to hum...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhang YJ,Hu XB,Li SX,Tian LP,Yang SL,Gong Y

更新日期：2004-06-01 00:00:00

abstract：:The plasminogen activator inhibitor type-2 (PAI-2) dependent apoptosis protection is due to the 33 amino acids fragment located between helix C and D of PAI-2, this fragment may interact with some unknown intracellular proteins. In this study we used the fragment between helix C and D of PAI-2 as a bait to perform a y...

journal_title：Acta biochimica et biophysica Sinica

authors： Fan J,Zhang YQ,Li P,Tong C,Tan L,Zhu YS

更新日期：2004-09-01 00:00:00

abstract：:Castration-resistant prostate cancer (CRPC) is fatal and there is currently no effective clinical treatment. The antibiotic doxycycline has shown anti-cancer effect in several kinds of solid tumors including prostate cancer. In this study, a combination of doxycycline and doxorubicin was used to investigate the synerg...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhu C,Yan X,Yu A,Wang Y

更新日期：2017-11-01 00:00:00

abstract：:RNA interference (RNAi), a posttranscriptional gene silencing process mediated by small double-stranded RNA specifically complementary to the targeted transcript, has been used extensively in the development of novel therapeutic approaches against various human diseases including chronic myelogenous leukemia (CML). He...

journal_title：Acta biochimica et biophysica Sinica

authors： Yang F,Zhang Y,Cao YL,Wang SH,Liu L

更新日期：2005-12-01 00:00:00

abstract：:Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endangered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hi...

journal_title：Acta biochimica et biophysica Sinica

authors： Jiang Y,Liu SZ,Zhang YL,Jiang MX,Sun QY,Chen DY

更新日期：2004-05-01 00:00:00

abstract：:Several essential biological progresses in mammals are regulated by circadian rhythms. Though the molecular mechanisms of oscillating these circadian rhythms have been uncovered, the specific functions of the circadian genes are not very clear. It has been reported that knocking down circadian genes by microRNA is a u...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhao X,Zhu X,Cheng S,Xie Y,Wang Z,Liu Y,Jiang Z,Xiao J,Guo H,Wang Y

更新日期：2014-04-01 00:00:00

abstract：:Pancreatic ductal adenocarcinoma (PDAC) is one of the most deadly human malignant diseases and the sixth leading cause of cancer-related deaths in China. Gemcitabine is the only first-line chemotherapeutic agent used for the palliative treatment of patients with PDAC, but chemo-resistance limits their efficacy. Here, ...

journal_title：Acta biochimica et biophysica Sinica

authors： Yao J,Li Z,Wang X,Xu P,Zhao L,Qian J

更新日期：2016-02-01 00:00:00

abstract：:Ischemia/reperfusion injury (IRI) is a major cause of acute kidney damage, which often occurs in deceased donor kidney transplants. Cathelicidin PR-39 peptide possesses anti-inflammatory and wound repair effects through tissue angiogenesis and anti-apoptosis. This study assessed the role of PR-39 in anti-apoptosis in ...

journal_title：Acta biochimica et biophysica Sinica

authors： Liu J,Xue W,Xiang H,Zheng J,Zhao Y,Jiao L,Jiao Z

更新日期：2016-08-01 00:00:00

abstract：:In this study, a mistletoe lectin (ML) was purified from Chinese mistletoe and the effect of this 60 kDa Chinese ML on human gammadelta T cell cytotoxicity, apoptosis and modulation of the cytokine network was studied. The cytotoxic properties of delta T cells was evaluated by using a (51)Cr release test and employed ...

journal_title：Acta biochimica et biophysica Sinica

authors： Gong F,Ma Y,Ma A,Yu Q,Zhang J,Nie H,Chen X,Shen B,Li N,Zhang D

更新日期：2007-06-01 00:00:00

abstract：:It has been reported that over-expression of GRP75 can protect cells under different types of stress. In this study, we investigated the protective effect of GRP75 on the liver both in vivo and in vitro. To evaluate the effect of GRP75 over-expression on oxidative damage in the liver in vitro, cell viability and the m...

journal_title：Acta biochimica et biophysica Sinica

authors： E Q,Liu X,Liu Y,Liu W,Zuo J

更新日期：2013-02-01 00:00:00

abstract：:The aim of this study is to construct a lentiviral expression vector containing a scavenger receptor (SR-PSOX) that binds with uniquely phosphatidylserine and oxidized lipoprotein with six histidine tags and to investigate the function of SR-PSOX in atherosclerosis. We utilize the ViraPower lentiviral expression syste...

journal_title：Acta biochimica et biophysica Sinica

authors： Quan Z,Yang H,Yang Y,Yan B,Cao R,Wen G,Liu C,Xu Y

更新日期：2007-03-01 00:00:00

abstract：:Ubiquitin E3 ligase membrane-associated RING-CH-type finger 7 (MARCH7), also known as axotrophin, was originally identified in mouse embryonic stem cells. MARCH7 is involved in T-cell proliferation, neuronal development, and the immune system. However, its role in endometrial cancer (EC) remains unclear. This study ai...

journal_title：Acta biochimica et biophysica Sinica

authors： Liu L,Hu J,Yu T,You S,Zhang Y,Hu L

更新日期：2019-05-23 00:00:00

abstract：:Two full-length cDNAs, named CaM1a and CaM1b, encoding the highly conserved calmodulin1 (CaM1) proteins, were isolated from the cDNA library of amphioxus Branchiostoma belcheri tsingtauense. There are only two nucleotide differences between them, producing one amino acid difference between CaM1a and CaM1b. Comparison ...

journal_title：Acta biochimica et biophysica Sinica

authors： Luan J,Liu Z,Zhang S,Li H,Fan C,Li L

更新日期：2007-04-01 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12), a key enzyme of carbon metabolism, was purified and characterized to homogeneity from skeletal muscle of Camelus dromedarius. The protein was purified approximately 26.8 folds by conventional ammonium sulphate fractionation followed by Blue Sepharose CL-6...

journal_title：Acta biochimica et biophysica Sinica

authors： Fourrat L,Iddar A,Soukri A

更新日期：2007-02-01 00:00:00

abstract：:In the present study, we tested the effect of cold water stress (CWS) on dopaminergic neurons in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease (PD) mouse model, and found that CWS pretreatment elicited less MPTP neurotoxicity. To understand the molecular mechanism underlying this phen...

journal_title：Acta biochimica et biophysica Sinica

authors： Xia M,Bian M,Yu Q,Liu J,Huang Y,Jin X,Lu S,Yu M,Huang F

更新日期：2011-06-01 00:00:00

abstract：:Tracking the distribution and differentiation of stem cells by high-resolution imaging techniques would have significant clinical and research implications. In this study, a model cell-penetrating peptide was used to carry gadolinium particles for magnetic resonance imaging (MRI) of mesenchymal stem cells (MSCs). MSCs...

journal_title：Acta biochimica et biophysica Sinica

authors： Liu M,Guo YM,Yang JL,Wang P,Zhao LY,Shen N,Wang SC,Guo XJ,Wu QF

更新日期：2006-12-01 00:00:00

abstract：:The effect of the guanosine triphosphatase activating proteins (GAPs) on spermatogenesis has been studied for years, though no GAPs have been explored in epididymis, an essential organ for sperm maturation. In this study, a new GAP member, designated as MacGAP, was cloned in human epididymis. The MacGAP gene encodes a...

journal_title：Acta biochimica et biophysica Sinica

authors： Li X,Liu Q,Liu S,Zhang J,Zhang Y

更新日期：2008-10-01 00:00:00

abstract：:Mutant N-terminal huntingtin (Htt) protein resulting from Huntington's disease (HD) with expanded polyglutamine accumulates and forms aggregates in vulnerable neurons. Both ubiquitin proteasomal and autophagic pathways contribute to the degradation of mutant Htt. Here, we focus on the involvement of chaperone-mediated...

journal_title：Acta biochimica et biophysica Sinica

authors： Qi L,Zhang XD

更新日期：2014-02-01 00:00:00

abstract：:The subfamily of voltage-dependent potassium (Kv) channel interacting protein 4 (KChIP4) is made up of the auxiliary interacting protein of voltage-dependent potassium channels. In this study, the structure of four splicing variants of the human KChIP4 gene was analyzed. Three of the four isoforms of the KChIP4 gene, ...

journal_title：Acta biochimica et biophysica Sinica

authors： Deng XY,Cai F,Xia K,Pan Q,Long ZG,Wu LQ,Liang DS,Dai HP,Zhang ZH,Xia JH

更新日期：2005-04-01 00:00:00

abstract：:PKZ, protein kinase containing Z-DNA domains, is a novel member of the vertebrate eIF2α kinase family. Containing a catalytic domain in C-terminus and two Z-DNA binding domains (Zα1 and Zα2) in N-terminus, PKZ can be activated through the binding of Zα to Z-DNA. However, the regulatory function of PKZ Zα remains to be...

journal_title：Acta biochimica et biophysica Sinica

authors： Lu P,Deng S,Wu C,Zhu Y,Liu Y,Lin G,Yan Y,Hu C

更新日期：2013-12-01 00:00:00