Abstract:
:Tracking the distribution and differentiation of stem cells by high-resolution imaging techniques would have significant clinical and research implications. In this study, a model cell-penetrating peptide was used to carry gadolinium particles for magnetic resonance imaging (MRI) of mesenchymal stem cells (MSCs). MSCs were isolated from rat bone marrow and identified by osteogenic differentiation in vitro. The cell-penetrating peptide labeled with fluorescein-5-isothiocyanate (FITC) and gadolinium was synthesized by a solid-phase peptide synthesis method. Fluorescein imaging analysis confirmed that this new peptide could internalize into the cytoplasm and nucleus at room temperature, 4 degrees and 37 degrees . Gadolinium were efficiently internalized into mesenchymal stem cells by the peptide in a time or concentration-dependent manner, resulting in intercellular shortening of longitudinal relaxation enhancements, which were obviously detected by 1.5 Tesla Magnetic Resonance Imaging. Cytotoxicity assay and flow cytometric analysis showed that the intercellular contrast medium incorporation did not affect cell viability at the tested concentrations. The in vitro experiment results suggested that the new constructed peptides could be a vector for tracking MSCs.
journal_name
Acta Biochim Biophys Sin (Shanghai)journal_title
Acta biochimica et biophysica Sinicaauthors
Liu M,Guo YM,Yang JL,Wang P,Zhao LY,Shen N,Wang SC,Guo XJ,Wu QFdoi
10.1111/j.1745-7270.2006.00239.xsubject
Has Abstractpub_date
2006-12-01 00:00:00pages
865-73issue
12eissn
1672-9145issn
1745-7270journal_volume
38pub_type
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