Construction of the seed-coat cDNA microarray and screening of differentially expressed genes in barley.

abstract：:The effect of the guanosine triphosphatase activating proteins (GAPs) on spermatogenesis has been studied for years, though no GAPs have been explored in epididymis, an essential organ for sperm maturation. In this study, a new GAP member, designated as MacGAP, was cloned in human epididymis. The MacGAP gene encodes a...

journal_title：Acta biochimica et biophysica Sinica

authors： Li X,Liu Q,Liu S,Zhang J,Zhang Y

更新日期：2008-10-01 00:00:00

abstract：:Melanoma is the most malignant and aggressive form of skin carcinoma originating in the pigment-producing melanocytes. In this study, to further investigate the molecular mechanisms of the development and progression of melanoma, we explored the impacts of long non-coding RNA (lncRNA) CASC2 on melanoma cell functions....

journal_title：Acta biochimica et biophysica Sinica

authors： Wang Z,Wang X,Zhou H,Dan X,Jiang L,Wu Y

更新日期：2018-03-01 00:00:00

abstract：:Canonical meiosis is characterized by two sequential rounds of nuclear divisions following one round of DNA replication-reductional segregation of homologous chromosomes during the first division and equational segregation of sister chromatids during the second division. Meiosis in an inverted order of two nuclear div...

journal_title：Acta biochimica et biophysica Sinica

authors： Li W,He X

更新日期：2020-07-10 00:00:00

abstract：:Both inhibitor of growth 4 (ING4) and phosphatase and tensin homolog deleted on chromosome 10 (PTEN) are well known as tumor suppressors that are closely related to tumor occurrence and progression. It was reported that ING4 and PTEN showed synergistic antitumor activities in nasopharyngeal carcinoma cells. The two tu...

journal_title：Acta biochimica et biophysica Sinica

authors： Rakshit N,Yang S,Zhou W,Xu Y,Deng C,Yang J,Yu H,Wei W

更新日期：2016-08-01 00:00:00

abstract：:The mechanisms of chronic neuropathic pain are not clear. Serum microRNAs (miRNAs) might show a special feature for chronic nervous lesions. However, little is known about the changes in circulating miRNAs for the neuropathic pain. Therefore, changes in the circulating miRNAs expression profile for the neuropathic pai...

journal_title：Acta biochimica et biophysica Sinica

authors： Xu Y,Zhang X,Pu S,Wu J,Lv Y,Du D

更新日期：2014-11-01 00:00:00

abstract：:To investigate whether apelin-13 induced THP-1 monocytes (MCs) adhesion to ECV304 human umbilical vein endothelial cells (HUVECs) via 14-3-3 signaling transduction pathway and the potential novel physiological function and signaling transduction pathway of apelin-APJ, HUVECs ECV304 were cultured in DMEM and MCs THP-1 ...

journal_title：Acta biochimica et biophysica Sinica

authors： Li X,Zhang X,Li F,Chen L,Li L,Qin X,Gao J,Su T,Zeng Y,Liao D

更新日期：2010-06-15 00:00:00

abstract：:Tracking the distribution and differentiation of stem cells by high-resolution imaging techniques would have significant clinical and research implications. In this study, a model cell-penetrating peptide was used to carry gadolinium particles for magnetic resonance imaging (MRI) of mesenchymal stem cells (MSCs). MSCs...

journal_title：Acta biochimica et biophysica Sinica

authors： Liu M,Guo YM,Yang JL,Wang P,Zhao LY,Shen N,Wang SC,Guo XJ,Wu QF

更新日期：2006-12-01 00:00:00

abstract：:MicroRNAs have been reported to be closely related to the development of human lung cancers. However, the functions of microRNAs in non-small cell lung cancer (NSCLC) remain largely undefined. Here, we investigated the role of microRNA-193b (miR-193b) in NSCLC. Our data showed that miR-193b was markedly down-regulated...

journal_title：Acta biochimica et biophysica Sinica

authors： Hu H,Li S,Liu J,Ni B

更新日期：2012-05-01 00:00:00

abstract：:To study the molecular basis of heterosis, suppression subtractive hybridization was used to investigate the differences in gene expression between porcine Longissimus dorsi of F1 hybrids Large WhitexMeishan and their female parents Meishan. From two specific subtractive cDNA libraries, the clones selected by reverse ...

journal_title：Acta biochimica et biophysica Sinica

authors： Huang T,Xiong YZ,Lei MG,Xu DQ,Deng CY

更新日期：2006-07-01 00:00:00

abstract：:Gallbladder cancer (GBC) is the most common and aggressive malignancy of the biliary tract. Betulinic acid (BetA) has been reported to have anti-inflammatory and antitumor effects; however, the effect of BetA on GBC is still unknown. In this study, we investigated the effect of BetA on five GBC cell lines and found th...

journal_title：Acta biochimica et biophysica Sinica

authors： Wang H,Dong F,Wang Y,Wang X,Hong D,Liu Y,Zhou J

更新日期：2020-02-03 00:00:00

abstract：:PKZ, protein kinase containing Z-DNA domains, is a novel member of the vertebrate eIF2α kinase family. Containing a catalytic domain in C-terminus and two Z-DNA binding domains (Zα1 and Zα2) in N-terminus, PKZ can be activated through the binding of Zα to Z-DNA. However, the regulatory function of PKZ Zα remains to be...

journal_title：Acta biochimica et biophysica Sinica

authors： Lu P,Deng S,Wu C,Zhu Y,Liu Y,Lin G,Yan Y,Hu C

更新日期：2013-12-01 00:00:00

abstract：:To explore new targets for hepatoma research, we used a surface display library to screen novel tumor cell-specific peptides. The bacterial FliTrx system was screened with living normal liver cell line L02 and hepatoma cell line HepG2 successively to search for hepatoma-specific peptides. Three clones (Hep1, Hep2, and...

journal_title：Acta biochimica et biophysica Sinica

authors： Li W,Lei P,Yu B,Wu S,Peng J,Zhao X,Zhu H,Kirschfink M,Shen G

更新日期：2008-05-01 00:00:00

abstract：:Transforming growth factor (TGF)-beta is a pleiotropic cytokine regulating a variety of cellular processes such as cell growth, differentiation, apoptosis, migration, cell adhesion, and immune response. In the well-understood classical TGF-beta signaling pathway, TGF-beta activates Smad signalling via its two cell sur...

journal_title：Acta biochimica et biophysica Sinica

authors： Yan X,Liu Z,Chen Y

更新日期：2009-04-01 00:00:00

abstract：:Mycobacterium tuberculosis (Mtb) is the key devastating bacterial pathogen responsible for tuberculosis. Increasing emergence of multi-drug-resistant, extensively drug-resistant, and rifampicin/isoniazid-resistant strains of Mtb makes the discovery of validated drug targets an urgent priority. As a vital translational...

journal_title：Acta biochimica et biophysica Sinica

authors： Yang J,Hong J,Luo L,Liu K,Meng C,Ji ZL,Lin D

更新日期：2019-02-01 00:00:00

abstract：:Proteomic techniques are promising strategies in the surveillance of hepatocellular carcinoma (HCC). This study aimed to investigate the serum profiling with magnetic bead (MB) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and to further identify the biomarkers for H...

journal_title：Acta biochimica et biophysica Sinica

authors： Liu T,Xue R,Huang X,Zhang D,Dong L,Wu H,Shen X

更新日期：2011-07-01 00:00:00

abstract：:RNA interference (RNAi), a posttranscriptional gene silencing process mediated by small double-stranded RNA specifically complementary to the targeted transcript, has been used extensively in the development of novel therapeutic approaches against various human diseases including chronic myelogenous leukemia (CML). He...

journal_title：Acta biochimica et biophysica Sinica

authors： Yang F,Zhang Y,Cao YL,Wang SH,Liu L

更新日期：2005-12-01 00:00:00

abstract：:hPFTAIRE1 is a Cdc2-related kinase family member. To search its substrates and regulatory proteins, hPFTAIRE1 was fused to LexA and used as a bait to screen a human brain LexA two-hybrid library. In this screening, seven hPFTAIRE1 interacting proteins, including promyelocytic leukemia zinc finger (PLZF), were obtained...

journal_title：Acta biochimica et biophysica Sinica

authors： Gao YK,Jiang M,Yang T,Chen JY

更新日期：2006-03-01 00:00:00

abstract：:At initial stages in the Rhizobium legume symbiosis, most nodulation genes are controlled by NodD protein and plant inducers. Some genetic studies and other reports have suggested that NodD may be activated by its direct interaction with plant inducers. However, there has been no molecular evidence of such an inducing...

journal_title：Acta biochimica et biophysica Sinica

authors： Li F,Hou B,Chen L,Yao Z,Hong G

更新日期：2008-09-01 00:00:00

abstract：:Increasing evidence has been accumulated indicating the important role of epigenetic regulation in tumor genesis. Previously, we observed that the transfection of hepatitis C virus core (HCVc) protein led to malignant transformation in normal biliary cells, and that tumor suppressor gene RASSF1A was downregulated in m...

journal_title：Acta biochimica et biophysica Sinica

authors： Guo N,Chen R,Li Z,Liu Y,Cheng D,Zhou Q,Zhou J,Lin Q

更新日期：2011-05-01 00:00:00

abstract：:A lectin with a novel N-terminal amino acid sequence was purified from the rhizomes of Aspidistra elatior Blume by ammonium sulphate precipitation, ion exchange chromatography on diethylaminoethyl-Sepharose and carboxymethyl-Sepharose and gel filtration chromatography on Sephacryl S-100. The A. elatior Blume lectin (A...

journal_title：Acta biochimica et biophysica Sinica

authors： Xu X,Wu C,Liu C,Luo Y,Li J,Zhao X,Damme EV,Bao J

更新日期：2007-07-01 00:00:00

abstract：:Apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development and tissue homeostasis. The progress of apoptosis is regulated in an orderly way by a series of signal cascades under certain circumstances. The caspase-cascade system plays vital roles in the induction...

journal_title：Acta biochimica et biophysica Sinica

authors： Fan TJ,Han LH,Cong RS,Liang J

更新日期：2005-11-01 00:00:00

abstract：:Reactive oxygen species (ROS), including H(2)O(2), play a critical role in the pathophysiology of osteoporosis. Therefore, agents or antioxidants that can inhibit ROS production have a high clinical value in the treatment of osteoporosis. Curculigoside (CUR), one of the main bioactive phenolic compounds isolated from ...

journal_title：Acta biochimica et biophysica Sinica

authors： Wang Y,Zhao L,Wang Y,Xu J,Nie Y,Guo Y,Tong Y,Qin L,Zhang Q

更新日期：2012-05-01 00:00:00

abstract：:Allelic loss of chromosome 3p, including the 3p21.3 region, is found in 95-100% of primary nasopharyngeal carcinoma (NPC) biopsies, suggesting that this region should harbor some tumor suppressor genes (TSGs) closely related to NPC development. Several TSGs located at 3p21.3, such as RASSF1A, LTF and BLU, have been de...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhou W,Feng X,Li H,Wang L,Zhu B,Liu W,Zhao M,Yao K,Ren C

更新日期：2009-01-01 00:00:00

abstract：:Several essential biological progresses in mammals are regulated by circadian rhythms. Though the molecular mechanisms of oscillating these circadian rhythms have been uncovered, the specific functions of the circadian genes are not very clear. It has been reported that knocking down circadian genes by microRNA is a u...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhao X,Zhu X,Cheng S,Xie Y,Wang Z,Liu Y,Jiang Z,Xiao J,Guo H,Wang Y

更新日期：2014-04-01 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12), a key enzyme of carbon metabolism, was purified and characterized to homogeneity from skeletal muscle of Camelus dromedarius. The protein was purified approximately 26.8 folds by conventional ammonium sulphate fractionation followed by Blue Sepharose CL-6...

journal_title：Acta biochimica et biophysica Sinica

authors： Fourrat L,Iddar A,Soukri A

更新日期：2007-02-01 00:00:00

abstract：:Activation of the P2X7 receptor leads to a rapid, bidirectional flux of cations, causing broad range of biological responses including cytotoxicity. However, the mechanism of P2X7-mediated cytotoxicity remains largely unexplored. In our previous study, the lack of P2X7-mediated calcium response under normal conditions...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhang X,Meng L,He B,Chen J,Liu P,Zhao J,Zhang Y,Li M,An D

更新日期：2009-05-01 00:00:00

abstract：:Osteosarcoma is the most common primary malignant bone tumor, which occurs in adolescents. As reported by our previous studies, HER4 indicates a poor prognosis of primary osteosarcoma. However, its mechanisms in the pathogenesis of osteosarcoma have not yet been studied. The purpose of this study was to investigate th...

journal_title：Acta biochimica et biophysica Sinica

authors： Li X,Huang Q,Wang S,Huang Z,Yu F,Lin J

更新日期：2020-04-20 00:00:00

abstract：:Ribosome-inactivating proteins (RIPs) are a group of toxic proteins that can specifically act on the universally conserved sarcin/ricin domain (S/R domain) of the largest RNA in ribosome and thus irreversibly inactivate ribosome for protein synthesis. Cinnamomin is a multifunctional type II RIP isolated in our laborat...

journal_title：Acta biochimica et biophysica Sinica

authors： Xu H,Liu WY

更新日期：2004-03-01 00:00:00

abstract：:Many N-methyl-d-aspartate (NMDA) receptor antagonists have been used to treat neurodegenerative diseases induced by glutamate excitotoxicity in clinics. However, the universality of the glutamic acid neurotransmitter system makes the glutamic acid receptor blockers inefficient and unsafe. Thus, regulating the downstre...

journal_title：Acta biochimica et biophysica Sinica

authors： Tian Y,He Y,Song W,Zhang E,Xia X

更新日期：2017-09-01 00:00:00

abstract：:Mediator complex subunit 1 (Med1)/Thyroid hormone receptor-associated protein 220 (TRAP220), an essential component of thyroid hormone receptor-associated proteins (TRAP)/mediator, plays important roles in hormone responses and tumorigenesis. However, the role of Med1 in the DNA damage response has not been studied. I...

journal_title：Acta biochimica et biophysica Sinica

authors： Kim HJ,Yun J

更新日期：2017-06-01 00:00:00