Abstract:
:In this study, we identified five miRNAs highly expressed in the small-cell lung cancer (SCLC) cell line NCI-H209. Among them, the expression levels of miR-375 were dramatically elevated in all SCLC cell lines examined, coincident with the expression of the transcription factor achaete-scute complex homolog 1 (ASCL1). Moreover, miR-375 was upregulated and correlated with ASCL1 in the cell lines generated from mouse SCLC-like tumors as well. Dual-luciferase assays further showed that ASCL1 activated the expression of miR-375 by binding to the three E-box elements in the miR-375 promoter. These results imply a role of ASCL1 in SCLC via the upregulation of miR-375.
journal_name
Acta Biochim Biophys Sin (Shanghai)journal_title
Acta biochimica et biophysica Sinicaauthors
Zhao H,Zhu L,Jin Y,Ji H,Yan X,Zhu Xdoi
10.1093/abbs/gmr110subject
Has Abstractpub_date
2012-02-01 00:00:00pages
177-82issue
2eissn
1672-9145issn
1745-7270pii
gmr110journal_volume
44pub_type
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