当前位置： SCI文献检索 > ACTA BIOCHIMICA ET BIOPHYSICA SINICA期刊下所有文献 > Effect of Mst1 overexpression on the growth of human hepatocellular carcinoma HepG2 cells and the sensitivity to cisplatin in vitro.

Effect of Mst1 overexpression on the growth of human hepatocellular carcinoma HepG2 cells and the sensitivity to cisplatin in vitro.

Abstract：

:Mammalian STE20-like kinase 1 (Mst1) is the mammalian homologue of Drosophila Hippo, a major inhibitor of cell proliferation in Drosophila. It ubiquitously encodes serine threonine kinase that belongs to the family of protein kinases related to yeast STE20, and is involved in cell proliferation, apoptosis, oncogenesis, and organ growth. Recent studies have shown that Mst1 has tumor-suppressor function, and the deletion or mutation of Mst1 is reported to be associated with tumorigenesis. To investigate the effect of overexpression of Mst1 on the growth of human liver cancer cell line HepG2 cells and the sensitivity to cisplatin in vitro, here we constructed recombinant eukaryotic expression vector pEGFP-N1-Mst1 containing Mst1 gene, and transiently transfected into HepG2 cells. The effects of Mst1 overexpression on the cell proliferation and apoptosis, the phosphorylation status of Yes-associated protein, and the mRNA transcript levels of connective tissue growth factor (CTGF), amphiregulin (AREG), and birc5 (Survivin) were determined. Results showed that overexpression of Mst1 inhibited cell proliferation, induced apoptosis of HepG2 cells, promoted YAP (Ser127) phosphorylation, and downregulated the mRNA expression of CTGF, AREG, and Survivin. We also investigated the relationship between the expression and cleavage of Mst1 and cisplatin-induced cell death. We found that Mst1 overexpression could induce cisplatin chemosensitivity, and cisplatin could promote the cleavage of Mst1 without affecting the expression of Mst1. Overall, our results indicated that Mst1 might be a promising anticancer target.

journal_name

Acta Biochim Biophys Sin (Shanghai)

journal_title

Acta biochimica et biophysica Sinica

authors

Xu C,Liu C,Huang W,Tu S,Wan F

doi

10.1093/abbs/gmt006

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

268-79

issue

4

eissn

1672-9145

issn

1745-7270

pii

gmt006

journal_volume

45

pub_type

杂志文章

相关文献

ACTA BIOCHIMICA ET BIOPHYSICA SINICA文献大全
  • Histone deacetylase SIRT6 inhibits glioma cell growth through down-regulating NOTCH3 expression.

    abstract：:Gliomas are the most common brain tumors of the central nervous system. In this study, we investigated the molecular mechanisms and biological function of SIRT6 in human gliomas. The expression levels of SIRT6 in glioma tissues and cells were analyzed by qRT-PCR and western blot analysis. CCK8 and clonogenicity assays...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmy019

    authors： Chen X,Li D,Gao Y,Cao Y,Hao B

    更新日期：2018-04-01 00:00:00

  • AMPKα1 regulates Idh2 transcription through H2B O-GlcNAcylation during brown adipogenesis.

    abstract：:AMP-activated protein kinase (AMPK) is indispensable for the development and maintenance of brown adipose tissue (BAT), and its activity is inhibited due to obesity. The isocitrate dehydrogenase 2 (IDH2) is a mitochondrial enzyme responsible for the production of α-ketoglutarate, a key intermediate metabolite integrat...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmaa136

    authors： Cao Y,Liu X,Zhao J,Du M

    更新日期：2021-01-12 00:00:00

  • Interrelationship between Alzheimer's disease and cardiac dysfunction: the brain-heart continuum?

    abstract：:Dementia, a devastating neurological disorder commonly found in the elderly, is characterized by severe cognitive and memory impairment. Ample clinical and epidemiological evidence has depicted a close association between dementia and heart failure. While cerebral blood under perfusion and neurohormonal activation due...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章,评审

    doi：10.1093/abbs/gmz115

    authors： Yang M,Li C,Zhang Y,Ren J

    更新日期：2020-01-02 00:00:00

  • Response of human non-small-cell lung cancer cells to the influence of Wogonin with SGK1 dynamics.

    abstract：:A number of significant studies in the field of cell biology have revealed another pattern of intracellular signal transduction in which cells transmit information through the dynamics of key signaling molecules. Dynamical properties of p53 have been demonstrated to be the key factor in dictating cell fate, including ...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmx006

    authors： Shi G,Wang Q,Zhou X,Li J,Liu H,Gu J,Wang H,Wu Y,Ding L,Ni S,Tang Z

    更新日期：2017-04-01 00:00:00

  • Effect of exogenous ghrelin on cell differentiation antigen 40 expression in endothelial cells.

    abstract：:Ghrelin is a brain-gut peptide that serves as a natural ligand for growth hormone secretagogue receptor (GHSR). It also exists abundantly in the cardiovascular system. In order to evaluate the possible role of ghrelin in the development of atherosclerosis, the effect of ghrelin on the expression of cell differentiatio...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2007.00365.x

    authors： Zhang M,Yuan F,Chen H,Qiu X,Fang W

    更新日期：2007-12-01 00:00:00

  • Purification and characterization of a mannose-binding lectin from the rhizomes of Aspidistra elatior Blume with antiproliferative activity.

    abstract：:A lectin with a novel N-terminal amino acid sequence was purified from the rhizomes of Aspidistra elatior Blume by ammonium sulphate precipitation, ion exchange chromatography on diethylaminoethyl-Sepharose and carboxymethyl-Sepharose and gel filtration chromatography on Sephacryl S-100. The A. elatior Blume lectin (A...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2007.00305.x

    authors： Xu X,Wu C,Liu C,Luo Y,Li J,Zhao X,Damme EV,Bao J

    更新日期：2007-07-01 00:00:00

  • Biological characteristics of dengue virus and potential targets for drug design.

    abstract：:Dengue infection is a major cause of morbidity in tropical and subtropical regions, bringing nearly 40% of the world population at risk and causing more than 20,000 deaths per year. But there is neither a vaccine for dengue disease nor antiviral drugs to treat the infection. In recent years, dengue infection has been ...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章,评审

    doi：10.1111/j.1745-7270.2008.00382.x

    authors： Qi RF,Zhang L,Chi CW

    更新日期：2008-02-01 00:00:00

  • Proteomic profiling of cotton fiber developmental transition from cell elongation to secondary wall deposition.

    abstract：:Cotton fiber developmental transition from elongation to secondary cell wall biosynthesis is a critical growth shifting phase that affects fiber final length, strength, and other properties. Morphological dynamic analysis indicated an asynchronous fiber developmental pattern between two most important commercial cotto...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmz111

    authors： Zhou X,Hu W,Li B,Yang Y,Zhang Y,Thow K,Fan L,Qu Y

    更新日期：2019-11-18 00:00:00

  • MiR-125a regulates chemo-sensitivity to gemcitabine in human pancreatic cancer cells through targeting A20.

    abstract：:Pancreatic ductal adenocarcinoma (PDAC) is one of the most deadly human malignant diseases and the sixth leading cause of cancer-related deaths in China. Gemcitabine is the only first-line chemotherapeutic agent used for the palliative treatment of patients with PDAC, but chemo-resistance limits their efficacy. Here, ...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmv129

    authors： Yao J,Li Z,Wang X,Xu P,Zhao L,Qian J

    更新日期：2016-02-01 00:00:00

  • A subunit vaccine based on rH-NS induces protection against Mycobacterium tuberculosis infection by inducing the Th1 immune response and activating macrophages.

    abstract：:Mycobacterium tuberculosis (Mtb) is a Gram-positive pathogen which causes tuberculosis in both animals and humans. All tested rH-NS formulations induced a specific Th1 response, as indicated by increased production of interferon γ (IFN-γ) and interleukin 2 (IL-2) by lymphocytes in the spleen of mice which were immuniz...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmw078

    authors： Liu Y,Chen S,Pan B,Guan Z,Yang Z,Duan L,Cai H

    更新日期：2016-10-01 00:00:00

  • Regulation of TGF-beta signaling by Smad7.

    abstract：:Transforming growth factor (TGF)-beta is a pleiotropic cytokine regulating a variety of cellular processes such as cell growth, differentiation, apoptosis, migration, cell adhesion, and immune response. In the well-understood classical TGF-beta signaling pathway, TGF-beta activates Smad signalling via its two cell sur...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章,评审

    doi：10.1093/abbs/gmp018

    authors： Yan X,Liu Z,Chen Y

    更新日期：2009-04-01 00:00:00

  • Silencing RhoA inhibits migration and invasion through Wnt/β-catenin pathway and growth through cell cycle regulation in human tongue cancer.

    abstract：:Ras homolog gene family member A (RhoA) has been identified as a critical regulator of tumor aggressive behavior. In this study, we assessed the role of RhoA in the mechanisms underlying growth, migration, and invasion of squamous cell carcinoma of tongue (TSCC). Stable RhoA knockdown of TSCC cell lines SCC-4 and CAL2...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmu051

    authors： Yan G,Zou R,Chen Z,Fan B,Wang Z,Wang Y,Yin X,Zhang D,Tong L,Yang F,Jiang W,Fu W,Zheng J,Bergo MO,Dalin M,Zheng J,Chen S,Zhou J

    更新日期：2014-08-01 00:00:00

  • Establishment and utilization of a tetracycline-controlled inducible RNA interfering system to repress gene expression in chronic myelogenous leukemia cells.

    abstract：:RNA interference (RNAi), a posttranscriptional gene silencing process mediated by small double-stranded RNA specifically complementary to the targeted transcript, has been used extensively in the development of novel therapeutic approaches against various human diseases including chronic myelogenous leukemia (CML). He...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2005.00112.x

    authors： Yang F,Zhang Y,Cao YL,Wang SH,Liu L

    更新日期：2005-12-01 00:00:00

  • Endothelial cells modified by adenovirus vector containing nine copies hypoxia response elements and human vascular endothelial growth factor as the novel seed cells for bone tissue engineering.

    abstract：:Vascularization is one of the hotspots during the development of new therapeutic strategies for bone tissue engineering, which can alleviate hypoxic circumstance and prevent transplant failure. Vascular endothelial growth factor (VEGF) gene transfection using recombinant adenovirus (Ad) vector can effectively promote ...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmx101

    authors： Song X,Shi L,Chen L,Liu X,Qu X,Wang K,Wei F

    更新日期：2017-11-01 00:00:00

  • Production of the polyclonal anti-human metallothionein 2A antibody with recombinant protein technology.

    abstract：:Metallothionein 2A (MT2A) is a small stress response protein that can be induced by exposure to toxic metals. It is highly expressed in breast cancer cells. In this study, the cDNA encoding the human MT2A protein was expressed as glutathione S-transferase (GST) fusion protein in Escherichia coli. Recombinant MT2A prot...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2006.00167.x

    authors： Marikar FM,Sun QM,Hua ZC

    更新日期：2006-05-01 00:00:00

  • Epitope mapping and identification on a 3D model built for the tartary buckwheat allergic protein TBb.

    abstract：:Allergic protein TBb, a major allergen in tartary buckwheat, was divided into four epitope-containing fragments and was named F1, F2, F3, and F4, respectively. Results of immunological assays revealed that F2 had the strongest IgE-binding activity to patient's sera, which indicated that it might contain the linear IgE...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmr036

    authors： Li P,Cui X,Li Y,Wang Z

    更新日期：2011-06-01 00:00:00

  • Analysis of the interaction between hPFTAIRE1 and PLZF in a yeast two-hybrid system.

    abstract：:hPFTAIRE1 is a Cdc2-related kinase family member. To search its substrates and regulatory proteins, hPFTAIRE1 was fused to LexA and used as a bait to screen a human brain LexA two-hybrid library. In this screening, seven hPFTAIRE1 interacting proteins, including promyelocytic leukemia zinc finger (PLZF), were obtained...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2006.00145.x

    authors： Gao YK,Jiang M,Yang T,Chen JY

    更新日期：2006-03-01 00:00:00

  • Atorvastatin protects cardiac progenitor cells from hypoxia-induced cell growth inhibition via MEG3/miR-22/HMGB1 pathway.

    abstract：:Heart failure (HF) induced by ischemia myocardial infarction (MI) is one of the major causes of morbidity and mortality all around the world. Atorvastatin, a hydroxymethylglutaryl coenzyme A reductase inhibitor, has been demonstrated to benefit patients with ischemic or non-ischemic-induced HF, but the mechanism is st...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmy133

    authors： Su J,Fang M,Tian B,Luo J,Jin C,Wang X,Ning Z,Li X

    更新日期：2018-12-01 00:00:00

  • Cloning, sequence analysis and identification of a nonsense mutation-mediated mRNA decay of porcine GSTM2 gene.

    abstract：:The glutathione S-transferase mu 2 gene (GSTM2) encodes a GST functioning in the elimination of electrophilic compounds and the regulation of cell growth. In this study, the sequence of porcine GSTM2 gene that contains the complete sequence encoding a protein of 218 amino acids was cloned. The deduced amino acid seque...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2007.00319.x

    authors： Huang J,Xiong Y,Deng C,Zuo B,Xu D,Lei M,Jiang S

    更新日期：2007-08-01 00:00:00

  • Snail1 is involved in de novo cardiac fibrosis after myocardial infarction in mice.

    abstract：:Epithelial-mesenchymal transition (EMT) is an important mechanism of cardiac fibrosis after myocardial infarction (MI). However, it remains unclear whether Snail1, an important regulator of EMT, is involved in cardiac fibrosis. In this study, we explored the expression patterns of Snail1 and a cardiac fibrosis marker-...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gms085

    authors： Liu Y,Du J,Zhang J,Weng M,Li X,Pu D,Gao L,Deng S,Xia S,She Q

    更新日期：2012-11-01 00:00:00

  • Ricin-B-lectin enhances microsporidia Nosema bombycis infection in BmN cells from silkworm Bombyx mori.

    abstract：:Nosema bombycis is an obligate intracellular parasitic fungus that utilizes a distinctive mechanism to infect Bombyx mori Spore germination can be used for host cell invasion; however, the detailed mechanism remains to be elucidated. The ricin-B-lectin (RBL) gene is significantly differentially regulated after N. bomb...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmw093

    authors： Liu H,Li M,Cai S,He X,Shao Y,Lu X

    更新日期：2016-11-01 00:00:00

  • Apoptotic mechanism of MCF-7 breast cells in vivo and in vitro induced by photodynamic therapy with C-phycocyanin.

    abstract：:The aim of this study was to investigate the pro-apoptotic mechanism of C-phycocyanin (C-PC)-mediated photodynamic therapy (PDT) in a murine tumor model and cultured MCF-7 cells. The mice were divided into four groups: control, He-Ne laser radiation, C-PC treatment, and C-PC treatment + He-Ne laser radiation. The effe...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmp104

    authors： Li B,Chu X,Gao M,Li W

    更新日期：2010-01-01 00:00:00

  • Role of chaperone-mediated autophagy in degrading Huntington's disease-associated huntingtin protein.

    abstract：:Mutant N-terminal huntingtin (Htt) protein resulting from Huntington's disease (HD) with expanded polyglutamine accumulates and forms aggregates in vulnerable neurons. Both ubiquitin proteasomal and autophagic pathways contribute to the degradation of mutant Htt. Here, we focus on the involvement of chaperone-mediated...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章,评审

    doi：10.1093/abbs/gmt133

    authors： Qi L,Zhang XD

    更新日期：2014-02-01 00:00:00

  • Identification of a differentially expressed gene PPP1CB between porcine Longissimus dorsi of Meishan and Large WhitexMeishan hybrids.

    abstract：:To study the molecular basis of heterosis, suppression subtractive hybridization was used to investigate the differences in gene expression between porcine Longissimus dorsi of F1 hybrids Large WhitexMeishan and their female parents Meishan. From two specific subtractive cDNA libraries, the clones selected by reverse ...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2006.00187.x

    authors： Huang T,Xiong YZ,Lei MG,Xu DQ,Deng CY

    更新日期：2006-07-01 00:00:00

  • Regulation and functions of the Hippo pathway in stemness and differentiation.

    abstract：:The Hippo pathway plays important roles in organ development, tissue regeneration, and human diseases, such as cancer. In the canonical Hippo pathway, the MST1/2-LATS1/2 kinase cascade phosphorylates and inhibits transcription coactivators Yes-associated protein and transcription coactivator with PDZ-binding motif and...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章,评审

    doi：10.1093/abbs/gmaa048

    authors： Cao X,Wang C,Liu J,Zhao B

    更新日期：2020-07-10 00:00:00

  • A new recombinant pituitary adenylate cyclase-activating peptide-derived peptide efficiently promotes glucose uptake and glucose-dependent insulin secretion.

    abstract：:The recombinant peptide, DBAYL, a promising therapeutic peptide for type 2 diabetes, is a new, potent, and highly selective agonist for VPAC2 generated through site-directed mutagenesis based on sequence alignments of pituitary adenylate cyclase-activating peptide (PACAP), vasoactive intestinal peptide (VIP), and rela...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gms078

    authors： Ma Y,Luo T,Xu W,Ye Z,Hong A

    更新日期：2012-11-01 00:00:00

  • Identification of a G-quadruplex forming sequence in the promoter of UCP1.

    abstract：:G-quadruplexes are higher-order nucleic acid structures formed in G-rich sequences in DNA or RNA. G-quadruplexes are distributed in many locations in the human genome, including promoter regions, and are viewed as promising therapeutic targets. Uncoupling protein-1 (UCP1) is a mitochondrial thermogenic gene critical f...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmy059

    authors： Zhao Y,Uhler JP

    更新日期：2018-07-01 00:00:00

  • Over-expression of GRP75 inhibits liver injury induced by oxidative damage.

    abstract：:It has been reported that over-expression of GRP75 can protect cells under different types of stress. In this study, we investigated the protective effect of GRP75 on the liver both in vivo and in vitro. To evaluate the effect of GRP75 over-expression on oxidative damage in the liver in vitro, cell viability and the m...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gms098

    authors： E Q,Liu X,Liu Y,Liu W,Zuo J

    更新日期：2013-02-01 00:00:00

  • Targeting AMPK signaling in combating ovarian cancers: opportunities and challenges.

    abstract：:The development and strategic application of effective anticancer therapies have turned out to be one of the most critical approaches of managing human cancers. Nevertheless, drug resistance is the major obstacle for clinical management of these diseases especially ovarian cancer. In the past years, substantial studie...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章,评审

    doi：10.1093/abbs/gmv128

    authors： Yung MM,Ngan HY,Chan DW

    更新日期：2016-04-01 00:00:00

  • Roles of cortactin, an actin polymerization mediator, in cell endocytosis.

    abstract：:Cortactin, an actin-binding protein and a substrate of Src, is encoded by the EMS1 oncogene. Cortactin is known to activate Arp2/3 complex-mediated actin polymerization and interact with dynamin, a large GTPase and proline rich domain-containing protein. Transferrin endocytosis was significantly reduced in cells by kn...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2006.00141.x

    authors： Chen L,Wang ZW,Zhu JW,Zhan X

    更新日期：2006-02-01 00:00:00