当前位置： SCI文献检索 > ACTA BIOCHIMICA ET BIOPHYSICA SINICA期刊下所有文献 > Ricin-B-lectin enhances microsporidia Nosema bombycis infection in BmN cells from silkworm Bombyx mori.

Ricin-B-lectin enhances microsporidia Nosema bombycis infection in BmN cells from silkworm Bombyx mori.

Abstract：

:Nosema bombycis is an obligate intracellular parasitic fungus that utilizes a distinctive mechanism to infect Bombyx mori Spore germination can be used for host cell invasion; however, the detailed mechanism remains to be elucidated. The ricin-B-lectin (RBL) gene is significantly differentially regulated after N. bombycis spore germination, and NbRBL might play roles in spore germination and infection. In this study, the biological function of NbRBL was examined. Protein sequence analysis showed that NbRBL is a secreted protein that attaches to carbohydrates. The relative expression level of the NbRBL gene was low during the first 30 h post-infection (hpi) in BmN cells, and high expression was detected from 42 hpi. Gene cloning, prokaryotic expression, and antibody preparation for NbRBL were performed. NbRBL was detected in total and secreted proteins using western blot analysis. Subcellular localization analysis showed that NbRBL is an intracellular protein. Spore adherence and infection assays showed that NbRBL could enhance spore adhesion to BmN cells; the proliferative activities of BmN cells incubated with anti-NbRBL were higher than those in negative control groups after N. bombycis infection; and the treatment groups showed less damage from spore invasion. We therefore, propose that NbRBL is released during spore germination, enhances spore adhesion to BmN cells, and contributes to spore invasion.

journal_name

Acta Biochim Biophys Sin (Shanghai)

journal_title

Acta biochimica et biophysica Sinica

authors

Liu H,Li M,Cai S,He X,Shao Y,Lu X

doi

10.1093/abbs/gmw093

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

1050-1057

issue

11

eissn

1672-9145

issn

1745-7270

pii

gmw093

journal_volume

48

pub_type

杂志文章

相关文献

ACTA BIOCHIMICA ET BIOPHYSICA SINICA文献大全
  • Adenovirus-mediated co-expression of ING4 and PTEN cooperatively enhances their antitumor activity in human hepatocellular carcinoma cells.

    abstract：:Both inhibitor of growth 4 (ING4) and phosphatase and tensin homolog deleted on chromosome 10 (PTEN) are well known as tumor suppressors that are closely related to tumor occurrence and progression. It was reported that ING4 and PTEN showed synergistic antitumor activities in nasopharyngeal carcinoma cells. The two tu...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmw062

    authors： Rakshit N,Yang S,Zhou W,Xu Y,Deng C,Yang J,Yu H,Wei W

    更新日期：2016-08-01 00:00:00

  • Histone deacetylase SIRT6 inhibits glioma cell growth through down-regulating NOTCH3 expression.

    abstract：:Gliomas are the most common brain tumors of the central nervous system. In this study, we investigated the molecular mechanisms and biological function of SIRT6 in human gliomas. The expression levels of SIRT6 in glioma tissues and cells were analyzed by qRT-PCR and western blot analysis. CCK8 and clonogenicity assays...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmy019

    authors： Chen X,Li D,Gao Y,Cao Y,Hao B

    更新日期：2018-04-01 00:00:00

  • New microbiota found in sputum from patients with community-acquired pneumonia.

    abstract：:Community-acquired pneumonia (CAP) is a major concern in hospitals and the bacterial community of which has not been systemically discussed yet. Sputum from patients in the acute stages is a kind of accessible sample reflecting its features. In our study, we analyzed 45 sputum samples from 45 patients with CAP. Eighte...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmt116

    authors： Chen C,Shen T,Tian F,Lin P,Li Q,Cui Z,Zhang Y,Xue M,Ye J,Guo X,Zhou Y

    更新日期：2013-12-01 00:00:00

  • Inverted meiosis: an alternative way of chromosome segregation for reproduction.

    abstract：:Canonical meiosis is characterized by two sequential rounds of nuclear divisions following one round of DNA replication-reductional segregation of homologous chromosomes during the first division and equational segregation of sister chromatids during the second division. Meiosis in an inverted order of two nuclear div...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章,评审

    doi：10.1093/abbs/gmaa054

    authors： Li W,He X

    更新日期：2020-07-10 00:00:00

  • The effects and regulatory mechanism of RIP3 on RGC-5 necroptosis following elevated hydrostatic pressure.

    abstract：:Necroptosis is a type of regulated cell death that has been implicated in various diseases. Receptor-interacting protein 3 (RIP3), a member of the RIP family, is an important mediator of the necroptotic pathway. Cleavage of RIP3 at Asp328 by caspase-8 abolishes the kinase activity of RIP3, which is critical for necrop...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmw130

    authors： Shang L,Ding W,Li N,Liao L,Chen D,Huang J,Xiong K

    更新日期：2017-02-06 00:00:00

  • Over-expression of GRP75 inhibits liver injury induced by oxidative damage.

    abstract：:It has been reported that over-expression of GRP75 can protect cells under different types of stress. In this study, we investigated the protective effect of GRP75 on the liver both in vivo and in vitro. To evaluate the effect of GRP75 over-expression on oxidative damage in the liver in vitro, cell viability and the m...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gms098

    authors： E Q,Liu X,Liu Y,Liu W,Zuo J

    更新日期：2013-02-01 00:00:00

  • Prognostic significance of VEGF-C expression in correlation with COX-2, lymphatic microvessel density, and clinicopathologic characteristics in human non-small cell lung cancer.

    abstract：:Lung cancer is one of the most lethal cancers in China because of high incidence and high mortality. Cyclooxygenase-2 (COX-2) and vessel endothelial growth factor C (VEGF-C) were found to play an important role in lymphangiogenesis of malignant tumors. In this study, we investigated whether lymphatic microvessel densi...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmp004

    authors： Guo X,Chen Y,Xu Z,Xu Z,Qian Y,Yu X

    更新日期：2009-03-01 00:00:00

  • Expression, purification and characterization of recombinant protein tyrosine phosphatase from Thermus thermophilus HB27.

    abstract：:The low-molecular-weight protein tyrosine phosphatases (PTPase) exist ubiquitously in prokaryotes and eukaryotes and play important roles in the regulation of physiological activities. We report here the expression, purification and characterization of an active and soluble PTPase from Thermus thermophilus HB27 in Esc...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmp057

    authors： Wang Y,Meng F,Zhang Y

    更新日期：2009-08-01 00:00:00

  • Endothelial cells modified by adenovirus vector containing nine copies hypoxia response elements and human vascular endothelial growth factor as the novel seed cells for bone tissue engineering.

    abstract：:Vascularization is one of the hotspots during the development of new therapeutic strategies for bone tissue engineering, which can alleviate hypoxic circumstance and prevent transplant failure. Vascular endothelial growth factor (VEGF) gene transfection using recombinant adenovirus (Ad) vector can effectively promote ...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmx101

    authors： Song X,Shi L,Chen L,Liu X,Qu X,Wang K,Wei F

    更新日期：2017-11-01 00:00:00

  • Kaempferol inhibits the growth and metastasis of cholangiocarcinoma in vitro and in vivo.

    abstract：:Kaempferol is a flavonoid that has been reported to exhibit antitumor activity in various malignant tumors. However, the role of kaempferol on cholangiocarcinoma (CCA) is largely unknown. In this article, we found that kaempferol inhibited proliferation, reduced colony formation ability, and induced apoptosis in HCCC9...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmv133

    authors： Qin Y,Cui W,Yang X,Tong B

    更新日期：2016-03-01 00:00:00

  • L-amino acid oxidase from Naja atra venom activates and binds to human platelets.

    abstract：:An L-amino acid oxidase (LAAO), NA-LAAO, was purified from the venom of Naja atra. Its N-terminal sequence shows great similarity with LAAOs from other snake venoms. NA-LAAO dose-dependently induced aggregation of washed human platelets. However, it had no activity on platelets in platelet-rich plasma. A low concentra...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2008.00372.x

    authors： Li R,Zhu S,Wu J,Wang W,Lu Q,Clemetson KJ

    更新日期：2008-01-01 00:00:00

  • LT(K63/R72), a new mutant of Escherichia coli heat-labile enterotoxin, exhibits characteristics more similar to LT(K63) than LT(R72).

    abstract：:LT(K63), a non-toxic mutant and LT(R72), a low toxic mutant of E. coli heat-labile enterotoxin are frequently used mucosal adjuvants. In many cases, the adjuvanticity of LT(K63) is lower than that of LT(R72), but LT(K63), which induces a mixed Th1/Th2 response, exhibits a higher level of protection than LT(R72) which ...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：

    authors： Feng Q,Yang J,Luo P,Zhang WJ,Zou QM

    更新日期：2005-02-01 00:00:00

  • Cloning and isolation of a Conus cysteine-rich protein homologous to Tex31 but without proteolytic activity.

    abstract：:We cloned and isolated a cysteine-rich protein, designated Mr30, from Conus marmoreus. Mr30 belongs to the cysteine-rich secretory protein family that is highly homologous to Tex31 previously obtained from Conus textile and reported as a protease responsible for processing of pro-conotoxins. Mr30, purified by a proced...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2008.00385.x

    authors： Qian J,Guo ZY,Chi CW

    更新日期：2008-02-01 00:00:00

  • Caspase family proteases and apoptosis.

    abstract：:Apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development and tissue homeostasis. The progress of apoptosis is regulated in an orderly way by a series of signal cascades under certain circumstances. The caspase-cascade system plays vital roles in the induction...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章,评审

    doi：10.1111/j.1745-7270.2005.00108.x

    authors： Fan TJ,Han LH,Cong RS,Liang J

    更新日期：2005-11-01 00:00:00

  • Identification and subcellular localization of molecular complexes of Gq/11α protein in HEK293 cells.

    abstract：:Heterotrimeric G-proteins localized in the plasma membrane convey the signals from G-protein-coupled receptors (GPCRs) to different effectors. At least some types of G-protein α subunits have been shown to be partly released from plasma membranes and to move into the cytosol after receptor activation by the agonists. ...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gms050

    authors： Drastichova Z,Novotny J

    更新日期：2012-08-01 00:00:00

  • Quality control system of the endoplasmic reticulum and related diseases.

    abstract：:The quality control (QC) system of the endoplasmic reticulum (ER) is an important monitoring mechanism in the protein maturation process, which ensures export of properly folded proteins from the ER. Incorrectly or incompletely folded proteins are retained in the ER for refolding or degradation by the ER-residing prot...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章,评审

    doi：10.1111/j.1745-7270.2006.00156.x

    authors： Wu JC,Liang ZQ,Qin ZH

    更新日期：2006-04-01 00:00:00

  • Purification and characterization of a mannose-binding lectin from the rhizomes of Aspidistra elatior Blume with antiproliferative activity.

    abstract：:A lectin with a novel N-terminal amino acid sequence was purified from the rhizomes of Aspidistra elatior Blume by ammonium sulphate precipitation, ion exchange chromatography on diethylaminoethyl-Sepharose and carboxymethyl-Sepharose and gel filtration chromatography on Sephacryl S-100. The A. elatior Blume lectin (A...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2007.00305.x

    authors： Xu X,Wu C,Liu C,Luo Y,Li J,Zhao X,Damme EV,Bao J

    更新日期：2007-07-01 00:00:00

  • Activation of liver X receptors promotes inflammatory cytokine mRNA degradation by upregulation of tristetraprolin.

    abstract：:Liver X receptors (LXRs) have anti-inflammatory properties. Whether LXRs play a role in post-transcriptional control of inflammatory cytokine expression is not clear. Here, we firstly identified that the synthetic LXR agonist T0901317 promoted IL-1β, IL-6 and TNFα mRNA degradation. Moreover, T0901317 destabilized TNFα...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmw136

    authors： Xiao J,Chen Q,Tang D,Ou W,Wang J,Mo Z,Tang C,Peng L,Wang D

    更新日期：2017-03-01 00:00:00

  • Effect of new berberine derivatives on colon cancer cells.

    abstract：:The natural alkaloid berberine has been recently described as a promising anticancer drug. In order to improve its efficacy and bioavailability, several derivatives have been designed and synthesized and found to be even more potent than the lead compound. Among the series of berberine derivatives we have produced, fi...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmv077

    authors： Guamán Ortiz LM,Croce AL,Aredia F,Sapienza S,Fiorillo G,Syeda TM,Buzzetti F,Lombardi P,Scovassi AI

    更新日期：2015-10-01 00:00:00

  • Hepatitis C virus core upregulates the methylation status of the RASSF1A promoter through regulation of SMYD3 in hilar cholangiocarcinoma cells.

    abstract：:Increasing evidence has been accumulated indicating the important role of epigenetic regulation in tumor genesis. Previously, we observed that the transfection of hepatitis C virus core (HCVc) protein led to malignant transformation in normal biliary cells, and that tumor suppressor gene RASSF1A was downregulated in m...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmr021

    authors： Guo N,Chen R,Li Z,Liu Y,Cheng D,Zhou Q,Zhou J,Lin Q

    更新日期：2011-05-01 00:00:00

  • Oleanolic acid induces apoptosis in human leukemia cells through caspase activation and poly(ADP-ribose) polymerase cleavage.

    abstract：:It has been shown that Fructus Ligustri Lucidi (FLL), a promising traditional Chinese medicine, can inhibit the growth of tumors. However, the effective component and molecular mechanism of FLL act to inhibit tumor proliferation are unclear. In this study, we demonstrated that oleanolic acid (OA), a principal chemical...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2007.00335.x

    authors： Zhang P,Li H,Chen D,Ni J,Kang Y,Wang S

    更新日期：2007-10-01 00:00:00

  • MicroRNA expression profile of gastric cancer stem cells in the MKN-45 cancer cell line.

    abstract：:MicroRNAs (miRNAs) play important roles in post-transcriptional gene silencing of target messenger RNAs, which are involved in virtually all biological processes. Previously, we have demonstrated that spheroid body-forming cells from the MKN-45 cancer cell line possessed gastric cancer stem cell (CSC) properties. In t...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmt135

    authors： Liu J,Ma L,Wang Z,Wang L,Liu C,Chen R,Zhang J

    更新日期：2014-02-01 00:00:00

  • Role of peroxisome proliferator-activated receptor gamma in glucose-induced insulin secretion.

    abstract：:Peroxisome proliferator-activated receptor (PPAR) isoforms (alpha and gamma) are known to be expressed in pancreatic islets as well as in insulin-producing cell lines. Ligands of PPAR have been shown to enhance glucose-induced insulin secretion in rat pancreatic islets. However, their effect on insulin secretion is st...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2006.00128.x

    authors： Xu ZK,Chen NG,Ma CY,Meng ZX,Sun YJ,Han X

    更新日期：2006-01-01 00:00:00

  • Kaempferol protects ethanol-induced gastric ulcers in mice via pro-inflammatory cytokines and NO.

    abstract：:Gastric ulcers (GUs) are common pathologies that affect many people around the world. Excessive alcohol consumption is one of the main causes of GUs; however, there are still lack of effective drugs for the prevention or therapy of GUs. In this study, we evaluated the protective effects and possible mechanisms of kaem...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmy002

    authors： Li Q,Hu X,Xuan Y,Ying J,Fei Y,Rong J,Zhang Y,Zhang J,Liu C,Liu Z

    更新日期：2018-03-01 00:00:00

  • Purification and characterization of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the dromedary camel.

    abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12), a key enzyme of carbon metabolism, was purified and characterized to homogeneity from skeletal muscle of Camelus dromedarius. The protein was purified approximately 26.8 folds by conventional ammonium sulphate fractionation followed by Blue Sepharose CL-6...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2007.00256.x

    authors： Fourrat L,Iddar A,Soukri A

    更新日期：2007-02-01 00:00:00

  • Metformin as an anti-cancer agent: actions and mechanisms targeting cancer stem cells.

    abstract：:Metformin, a first line medication for type II diabetes, initially entered the spotlight as a promising anti-cancer agent due to epidemiologic reports that found reduced cancer risk and improved clinical outcomes in diabetic patients taking metformin. To uncover the anti-cancer mechanisms of metformin, preclinical stu...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章,评审

    doi：10.1093/abbs/gmx106

    authors： Saini N,Yang X

    更新日期：2018-02-01 00:00:00

  • Effect of lentiviral vector-mediated KSR1 gene silencing on the proliferation of renal tubular epithelial cells and expression of inflammatory factors in a rat model of ischemia/reperfusion injury.

    abstract：:Renal ischemia/reperfusion (I/R) is a common cause of acute renal failure in many clinical settings. Our study aimed to elucidate the role of lentiviral vector-mediated KSR1 gene silencing in inflammatory factor expression and proliferation of renal tubular epithelial cells (RTECs) in a rat model of I/R injury. Male S...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmy071

    authors： Bai Y,Han G,Guo K,Yu L,Du X,Xu Y

    更新日期：2018-08-01 00:00:00

  • Alternative method for site-directed mutagenesis of complex polyketide synthase in Streptomyces albus JA3453.

    abstract：:Sequence analysis of oxazolomycin (OZM) biosynthetic gene cluster from Streptomyces albus JA3453 revealed a gene, ozmH, encoding a hybrid polyketide and non-ribosomal peptide enzyme. Tandem ketosynthase (KS) domains (KS 10-1 and KS 10-2) were characterized and they show significant homology with known KSs. Using an al...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2008.00408.x

    authors： Song D,Coughlin J,Ju J,Zhou X,Shen B,Zhao C,Deng Z

    更新日期：2008-04-01 00:00:00

  • Doxycycline synergizes with doxorubicin to inhibit the proliferation of castration-resistant prostate cancer cells.

    abstract：:Castration-resistant prostate cancer (CRPC) is fatal and there is currently no effective clinical treatment. The antibiotic doxycycline has shown anti-cancer effect in several kinds of solid tumors including prostate cancer. In this study, a combination of doxycycline and doxorubicin was used to investigate the synerg...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmx097

    authors： Zhu C,Yan X,Yu A,Wang Y

    更新日期：2017-11-01 00:00:00

  • Proteomic profiling of cotton fiber developmental transition from cell elongation to secondary wall deposition.

    abstract：:Cotton fiber developmental transition from elongation to secondary cell wall biosynthesis is a critical growth shifting phase that affects fiber final length, strength, and other properties. Morphological dynamic analysis indicated an asynchronous fiber developmental pattern between two most important commercial cotto...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmz111

    authors： Zhou X,Hu W,Li B,Yang Y,Zhang Y,Thow K,Fan L,Qu Y

    更新日期：2019-11-18 00:00:00