Expression, purification and characterization of recombinant protein tyrosine phosphatase from Thermus thermophilus HB27.

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been reported to interact with proteins containing the polyglutamine (polyQ) domain. The present study was undertaken to evaluate the potential contributions of the polyQ and polyproline (polyP) domains to the co-localization of mutant huntingtin (htt) and GAPDH. Ov...

journal_title：Acta biochimica et biophysica Sinica

authors： Wu J,Lin F,Qin Z

更新日期：2007-11-01 00:00:00

abstract：:Increasing evidence has been accumulated indicating the important role of epigenetic regulation in tumor genesis. Previously, we observed that the transfection of hepatitis C virus core (HCVc) protein led to malignant transformation in normal biliary cells, and that tumor suppressor gene RASSF1A was downregulated in m...

journal_title：Acta biochimica et biophysica Sinica

authors： Guo N,Chen R,Li Z,Liu Y,Cheng D,Zhou Q,Zhou J,Lin Q

更新日期：2011-05-01 00:00:00

abstract：:Canonical meiosis is characterized by two sequential rounds of nuclear divisions following one round of DNA replication-reductional segregation of homologous chromosomes during the first division and equational segregation of sister chromatids during the second division. Meiosis in an inverted order of two nuclear div...

journal_title：Acta biochimica et biophysica Sinica

authors： Li W,He X

更新日期：2020-07-10 00:00:00

abstract：:Inter-species nuclear transfer could be used to preserve North Goat (Capra ibex), an endangered species. We established the culture conditions for ibex-hirus reconstructed embryos and optimized the method for DNA extractions of a single cell and early cloned embryo. By using mitochondria-specific probes of ibex and hi...

journal_title：Acta biochimica et biophysica Sinica

authors： Jiang Y,Liu SZ,Zhang YL,Jiang MX,Sun QY,Chen DY

更新日期：2004-05-01 00:00:00

abstract：:Ubiquitin E3 ligase membrane-associated RING-CH-type finger 7 (MARCH7), also known as axotrophin, was originally identified in mouse embryonic stem cells. MARCH7 is involved in T-cell proliferation, neuronal development, and the immune system. However, its role in endometrial cancer (EC) remains unclear. This study ai...

journal_title：Acta biochimica et biophysica Sinica

authors： Liu L,Hu J,Yu T,You S,Zhang Y,Hu L

更新日期：2019-05-23 00:00:00

abstract：:MicroRNAs (miRNAs) are some short RNAs that regulate multiple biological functions at post-transcriptional levels, such as tumorigenic processes, inflammatory lesions and cell apoptosis. Zinc finger E-box binding homeobox factor 1 (ZEB1) is a crucial mediator of epithelial-mesenchymal transition (EMT). It induces mali...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhao L,Li R,Xu S,Li Y,Zhao P,Dong W,Liu Z,Zhao Q,Tan B

更新日期：2018-02-01 00:00:00

abstract：:In the present study, paclitaxel (PTX) were encapsulated with polyethylene glycol (PEG)-polylactide (PLA)/D-α tocopheryl polyethylene glycol 1000 succinate (TPGS) (PEG-PLA/TPGS) and the enhanced anti-tumor activity of this PTX mixed micelles (PTX-MM) was evaluated in lung cancer cells. The PTX-MM prepared by a solvent...

journal_title：Acta biochimica et biophysica Sinica

authors： Huang G,Zang B,Wang X,Liu G,Zhao J

更新日期：2015-12-01 00:00:00

abstract：:Aberrant microRNA (miRNA) expression plays a critical role in osteosarcoma (OS) pathogenesis. In this study, we elucidated the involvement of miR-487a in OS and the underlying molecular mechanisms. We found that miR-487a was upregulated in OS clinical samples and cell lines. Knockdown of miR-487a suppressed OS cell gr...

journal_title：Acta biochimica et biophysica Sinica

authors： Gu Z,Wu S,Xu G,Wu W,Mao B,Zhao S

更新日期：2020-06-20 00:00:00

abstract：:Human-induced pluripotent stem (iPS) cells share the same key properties as embryonic stem cells, and may be generated from patient- or disease-specific sources, which makes them attractive for personalized medicine, drug screens, or cellular therapy. Long-term cultivation and maintenance of normal iPS cells in an und...

journal_title：Acta biochimica et biophysica Sinica

authors： Liu T,Chen Q,Huang Y,Huang Q,Jiang L,Guo L

更新日期：2012-03-01 00:00:00

abstract：:Castration-resistant prostate cancer (CRPC) is fatal and there is currently no effective clinical treatment. The antibiotic doxycycline has shown anti-cancer effect in several kinds of solid tumors including prostate cancer. In this study, a combination of doxycycline and doxorubicin was used to investigate the synerg...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhu C,Yan X,Yu A,Wang Y

更新日期：2017-11-01 00:00:00

abstract：:We previously identified a matrix protein, MSI7, from pearl oyster Pinctada fucata. According to the structural analysis, the DGD site in the N-terminal of MSI7 is crucial for its role in the shell formation. In this study, we expressed a series of recombinant MSI7 proteins, including the wild-type and several mutants...

journal_title：Acta biochimica et biophysica Sinica

authors： Feng Q,Fang Z,Yan Z,Xing R,Xie L,Zhang R

更新日期：2009-11-01 00:00:00

abstract：:Trehalose-6-phosphate phosphatase (TPP) is an essential enzyme for growth of mycobacteria, which has been identified to be a potential anti-tuberculosis drug target. However, the biochemical and ligand-binding properties and the 3D structure of TPP remain unclear so far. In the present study, we expressed the recombin...

journal_title：Acta biochimica et biophysica Sinica

authors： Shi L,Zhang H,Qiu Y,Wang Q,Wu X,Wang H,Zhang X,Lin D

更新日期：2013-10-01 00:00:00

abstract：:Several essential biological progresses in mammals are regulated by circadian rhythms. Though the molecular mechanisms of oscillating these circadian rhythms have been uncovered, the specific functions of the circadian genes are not very clear. It has been reported that knocking down circadian genes by microRNA is a u...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhao X,Zhu X,Cheng S,Xie Y,Wang Z,Liu Y,Jiang Z,Xiao J,Guo H,Wang Y

更新日期：2014-04-01 00:00:00

abstract：:Gallbladder cancer (GBC) is the most common and aggressive malignancy of the biliary tract. Betulinic acid (BetA) has been reported to have anti-inflammatory and antitumor effects; however, the effect of BetA on GBC is still unknown. In this study, we investigated the effect of BetA on five GBC cell lines and found th...

journal_title：Acta biochimica et biophysica Sinica

authors： Wang H,Dong F,Wang Y,Wang X,Hong D,Liu Y,Zhou J

更新日期：2020-02-03 00:00:00

abstract：:Community-acquired pneumonia (CAP) is a major concern in hospitals and the bacterial community of which has not been systemically discussed yet. Sputum from patients in the acute stages is a kind of accessible sample reflecting its features. In our study, we analyzed 45 sputum samples from 45 patients with CAP. Eighte...

journal_title：Acta biochimica et biophysica Sinica

authors： Chen C,Shen T,Tian F,Lin P,Li Q,Cui Z,Zhang Y,Xue M,Ye J,Guo X,Zhou Y

更新日期：2013-12-01 00:00:00

abstract：:Activation of the P2X7 receptor leads to a rapid, bidirectional flux of cations, causing broad range of biological responses including cytotoxicity. However, the mechanism of P2X7-mediated cytotoxicity remains largely unexplored. In our previous study, the lack of P2X7-mediated calcium response under normal conditions...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhang X,Meng L,He B,Chen J,Liu P,Zhao J,Zhang Y,Li M,An D

更新日期：2009-05-01 00:00:00

abstract：:Galectin-3 (Gal-3) which shows affinity of β-galactosides is a cancer-related protein. Thus, it is important to understand its ligand binding mechanism and then design its specific inhibitor. It was suggested that the positions of water molecules in Gal-3 ligand-binding site could be replaced by appropriate chemical g...

journal_title：Acta biochimica et biophysica Sinica

authors： Su J,Zhang T,Wang P,Liu F,Tai G,Zhou Y

更新日期：2015-03-01 00:00:00

abstract：:Posttranslational modifications (PTMs) of histone proteins, such as acetylation, methylation, phosphorylation, and ubiquitylation, play essential roles in regulating chromatin dynamics. Combinations of different modifications on the histone proteins, termed 'histone code' in many cases, extend the information potentia...

journal_title：Acta biochimica et biophysica Sinica

authors： Zhang X,Wen H,Shi X

更新日期：2012-01-01 00:00:00

abstract：:Two full-length cDNAs, named CaM1a and CaM1b, encoding the highly conserved calmodulin1 (CaM1) proteins, were isolated from the cDNA library of amphioxus Branchiostoma belcheri tsingtauense. There are only two nucleotide differences between them, producing one amino acid difference between CaM1a and CaM1b. Comparison ...

journal_title：Acta biochimica et biophysica Sinica

authors： Luan J,Liu Z,Zhang S,Li H,Fan C,Li L

更新日期：2007-04-01 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is a fascinating target for cancer therapy due to its significant role in the crossroad of multiple signaling pathways associated with cell proliferation and regulation. Hsp90 inhibitors have the potential to be developed into anti-cancer drugs. Here, we identified nicotinic-mycoepoxydien...

journal_title：Acta biochimica et biophysica Sinica

authors： Sun Y,Xiao S,Chen J,Wang M,Zheng Z,Song S,Zhang L

更新日期：2015-06-01 00:00:00

abstract：:Cumulative work on glucocorticoid (GC) regulation of genes in lymphoid cell cultures has revealed that apoptotic sensitivity to GCs depends on sufficient active GC receptors in the cells. The actions of the ligand-driven GC receptor that lead to apoptosis depend on interactions with other major cell-signaling systems,...

journal_title：Acta biochimica et biophysica Sinica

authors： Thompson EB

更新日期：2008-07-01 00:00:00

abstract：:The unique temporal expression pattern of miR-200c in epididymis during post-natal development in juvenile rats was revealed by our home-made miRNA microarray in this paper. It was found that miR-200c expressed in the lowest level at Day 7 and then increased to the highest at Day 36 followed by a dramatic decrease. Th...

journal_title：Acta biochimica et biophysica Sinica

authors： Wang J,Ruan K

更新日期：2010-09-01 00:00:00

abstract：:Osteoarthritis (OA) is an age-related joint disease that is characterized by the degeneration of articular chondrocytes. Nuclear enzyme poly(ADP-ribose) polymerase 1 (PARP-1) is associated with inflammation response. We investigated the role of PARP-1 in interleukin-1β (IL-1β)-stimulated human articular chondrocytes a...

journal_title：Acta biochimica et biophysica Sinica

authors： Sun Y,Zhou L,Lv D,Liu H,He T,Wang X

更新日期：2015-06-01 00:00:00

abstract：:In this study, we confirmed that sesamin, an active lignan isolated from sesame seed and oil, is a novel skin-tanning compound. The melanin content and tyrosinase activity were increased by sesamin in a dose-dependent manner in B16 melanoma cells. The mRNA and protein levels of tyrosinase were also enhanced after the ...

journal_title：Acta biochimica et biophysica Sinica

authors： Jiang Z,Li S,Liu Y,Deng P,Huang J,He G

更新日期：2011-10-01 00:00:00

abstract：:Luffin-a, a single-chain Type I ribosome-inactivating protein, which is known to be the most toxic of the luffin family and apparently possesses antitumor activity, was isolated from Luffa cylindrica seeds. In the present study, mature alpha-luffin was cloned from L. cylindrica and it was found that mature alpha-luffi...

journal_title：Acta biochimica et biophysica Sinica

authors： Liu L,Wang R,He W,He F,Huang G

更新日期：2010-08-01 00:00:00

abstract：:The aim of this study was to investigate the pro-apoptotic mechanism of C-phycocyanin (C-PC)-mediated photodynamic therapy (PDT) in a murine tumor model and cultured MCF-7 cells. The mice were divided into four groups: control, He-Ne laser radiation, C-PC treatment, and C-PC treatment + He-Ne laser radiation. The effe...

journal_title：Acta biochimica et biophysica Sinica

authors： Li B,Chu X,Gao M,Li W

更新日期：2010-01-01 00:00:00

abstract：:Melanoma is the most malignant and aggressive form of skin carcinoma originating in the pigment-producing melanocytes. In this study, to further investigate the molecular mechanisms of the development and progression of melanoma, we explored the impacts of long non-coding RNA (lncRNA) CASC2 on melanoma cell functions....

journal_title：Acta biochimica et biophysica Sinica

authors： Wang Z,Wang X,Zhou H,Dan X,Jiang L,Wu Y

更新日期：2018-03-01 00:00:00

abstract：:Emerging evidence suggests that specific spatio-temporal microRNA (miRNA) expression is required for heart development. In recent years, hundreds of miRNAs have been discovered. In contrast, functional annotations are available only for a very small fraction of these regulatory molecules. In order to provide a global ...

journal_title：Acta biochimica et biophysica Sinica

authors： Liu G,Ding M,Chen J,Huang J,Wang H,Jing Q,Shen B

更新日期：2010-09-01 00:00:00

abstract：:Renal ischemia/reperfusion (I/R) is a common cause of acute renal failure in many clinical settings. Our study aimed to elucidate the role of lentiviral vector-mediated KSR1 gene silencing in inflammatory factor expression and proliferation of renal tubular epithelial cells (RTECs) in a rat model of I/R injury. Male S...

journal_title：Acta biochimica et biophysica Sinica

authors： Bai Y,Han G,Guo K,Yu L,Du X,Xu Y

更新日期：2018-08-01 00:00:00

abstract：:Hyperglycemia, a diagnostic characteristic of diabetes mellitus, is detrimental to pancreatic β cells. Delphinidin, a member of the anthocyanin family, inhibits glucose absorption, increases glucagon-like peptide-1 (GLP-1) secretion, and improves insulin secretion in diabetes. However, whether delphinidin plays a prot...

journal_title：Acta biochimica et biophysica Sinica

authors： Lai D,Huang M,Zhao L,Tian Y,Li Y,Liu D,Wu Y,Deng F

更新日期：2019-12-13 00:00:00