当前位置： SCI文献检索 > ACTA BIOCHIMICA ET BIOPHYSICA SINICA期刊下所有文献 > Identification and subcellular localization of molecular complexes of Gq/11α protein in HEK293 cells.

Identification and subcellular localization of molecular complexes of Gq/11α protein in HEK293 cells.

Abstract：

:Heterotrimeric G-proteins localized in the plasma membrane convey the signals from G-protein-coupled receptors (GPCRs) to different effectors. At least some types of G-protein α subunits have been shown to be partly released from plasma membranes and to move into the cytosol after receptor activation by the agonists. However, the mechanism underlying subcellular redistribution of trimeric G-proteins is not well understood and no definitive conclusions have been reached regarding the translocation of Gα subunits between membranes and cytosol. Here we used subcellular fractionation and clear-native polyacrylamide gel electrophoresis to identify molecular complexes of G(q/11)α protein and to determine their localization in isolated fractions and stability in naïve and thyrotropin-releasing hormone (TRH)-treated HEK293 cells expressing high levels of TRH receptor and G(11)α protein. We identified two high-molecular-weight complexes of 300 and 140 kDa in size comprising the G(q/11) protein, which were found to be membrane-bound. Both of these complexes dissociated after prolonged treatment with TRH. Still other G(q/11)α protein complexes of lower molecular weight were determined in the cytosol. These 70 kDa protein complexes were barely detectable under control conditions but their levels markedly increased after prolonged (4-16 h) hormone treatment. These results support the notion that a portion of G(q/11)α can undergo translocation from the membrane fraction into soluble fraction after a long-term activation of TRH receptor. At the same time, these findings indicate that the redistribution of G(q/11)α is brought about by the dissociation of high-molecular-weight complexes and concomitant formation of low-molecular-weight complexes containing the G(q/11)α protein.

journal_name

Acta Biochim Biophys Sin (Shanghai)

journal_title

Acta biochimica et biophysica Sinica

authors

Drastichova Z,Novotny J

doi

10.1093/abbs/gms050

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

641-9

issue

8

eissn

1672-9145

issn

1745-7270

pii

gms050

journal_volume

44

pub_type

杂志文章

相关文献

ACTA BIOCHIMICA ET BIOPHYSICA SINICA文献大全
  • Leptin promotes fatty acid oxidation and OXPHOS via the c-Myc/PGC-1 pathway in cancer cells.

    abstract：:Alteration in cellular energy metabolism plays a critical role in the development and progression of cancer. Leptin is a hormone secreted by adipose tissue. Recent reports have shown that leptin can induce cancer cell proliferation and regulate cell energy metabolism, but the regulatory mechanism is still unclear. Her...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmz058

    authors： Liu Q,Sun Y,Fei Z,Yang Z,Duan K,Zi J,Cui Q,Yu M,Xiong W

    更新日期：2019-07-10 00:00:00

  • Optimized adaptor polymerase chain reaction method for efficient genomic walking.

    abstract：:Genomic walking is one of the most useful approaches in genome-related research. Three kinds of PCR-based methods are available for this purpose. However, none of them has been generally applied because they are either insensitive or inefficient. Here we present an efficient PCR protocol, an optimized adaptor PCR meth...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2006.00194.x

    authors： Xu P,Hu RY,Ding XY

    更新日期：2006-08-01 00:00:00

  • Atorvastatin protects cardiac progenitor cells from hypoxia-induced cell growth inhibition via MEG3/miR-22/HMGB1 pathway.

    abstract：:Heart failure (HF) induced by ischemia myocardial infarction (MI) is one of the major causes of morbidity and mortality all around the world. Atorvastatin, a hydroxymethylglutaryl coenzyme A reductase inhibitor, has been demonstrated to benefit patients with ischemic or non-ischemic-induced HF, but the mechanism is st...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmy133

    authors： Su J,Fang M,Tian B,Luo J,Jin C,Wang X,Ning Z,Li X

    更新日期：2018-12-01 00:00:00

  • Checkpoint-dependent phosphorylation of Med1/TRAP220 in response to DNA damage.

    abstract：:Mediator complex subunit 1 (Med1)/Thyroid hormone receptor-associated protein 220 (TRAP220), an essential component of thyroid hormone receptor-associated proteins (TRAP)/mediator, plays important roles in hormone responses and tumorigenesis. However, the role of Med1 in the DNA damage response has not been studied. I...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmx036

    authors： Kim HJ,Yun J

    更新日期：2017-06-01 00:00:00

  • Regulation of TGF-beta signaling by Smad7.

    abstract：:Transforming growth factor (TGF)-beta is a pleiotropic cytokine regulating a variety of cellular processes such as cell growth, differentiation, apoptosis, migration, cell adhesion, and immune response. In the well-understood classical TGF-beta signaling pathway, TGF-beta activates Smad signalling via its two cell sur...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章,评审

    doi：10.1093/abbs/gmp018

    authors： Yan X,Liu Z,Chen Y

    更新日期：2009-04-01 00:00:00

  • Beneficial effects of C-peptide on renal morphology in diabetic rats.

    abstract：:The study was undertaken to examine the effects of C-peptide on glomerular volume (V(GLOM)), mesangial matrix synthesis, and degradation in streptozotocin (STZ)-diabetic rats with poor or moderate glycemic control. Series 1 (poor glycemic control) included groups of healthy rats, hyperglycemic rats, diabetic insulin-t...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmq100

    authors： Sun W,Gao X,Zhao X,Cui D,Xia Q

    更新日期：2010-12-01 00:00:00

  • Adiponectin attenuates lipopolysaccharide-induced cell injury of H9c2 cells by regulating AMPK pathway.

    abstract：:Adiponectin, an adipokine synthesized and secreted majorly by adipose tissue, is reported to exert cardioprotective properties via anti-inflammation and antiapoptosis. Lipopolysaccharide (LPS) is a common inflammation and apoptosis inducer of cardiomyocytes. However, few studies have reported the roles of adiponectin ...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmy162

    authors： Hao R,Su G,Sun X,Kong X,Zhu C,Su G

    更新日期：2019-02-01 00:00:00

  • Response of human non-small-cell lung cancer cells to the influence of Wogonin with SGK1 dynamics.

    abstract：:A number of significant studies in the field of cell biology have revealed another pattern of intracellular signal transduction in which cells transmit information through the dynamics of key signaling molecules. Dynamical properties of p53 have been demonstrated to be the key factor in dictating cell fate, including ...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmx006

    authors： Shi G,Wang Q,Zhou X,Li J,Liu H,Gu J,Wang H,Wu Y,Ding L,Ni S,Tang Z

    更新日期：2017-04-01 00:00:00

  • Delivery of Tapasin-modified CTL epitope peptide via cytoplasmic transduction peptide induces CTLs by JAK/STAT signaling pathway in vivo.

    abstract：:Hepatitis B virus (HBV)-specific cytotoxic T lymphocytes (CTLs) play a vital role in viral control and clearance. Recent studies have elucidated that Tapasin, an endoplasmic reticulum chaperone, is a well-known molecule that appears to be essential in peptide-loading process. The Janus kinase/signal transducers and ac...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmx133

    authors： Wu S,Chen X,Tang Y,Zhang Y,Li D,Chen J,Wang J,Tang Z,Zang G,Yu Y

    更新日期：2018-02-01 00:00:00

  • The water network in galectin-3 ligand binding site guides inhibitor design.

    abstract：:Galectin-3 (Gal-3) which shows affinity of β-galactosides is a cancer-related protein. Thus, it is important to understand its ligand binding mechanism and then design its specific inhibitor. It was suggested that the positions of water molecules in Gal-3 ligand-binding site could be replaced by appropriate chemical g...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmu132

    authors： Su J,Zhang T,Wang P,Liu F,Tai G,Zhou Y

    更新日期：2015-03-01 00:00:00

  • New microbiota found in sputum from patients with community-acquired pneumonia.

    abstract：:Community-acquired pneumonia (CAP) is a major concern in hospitals and the bacterial community of which has not been systemically discussed yet. Sputum from patients in the acute stages is a kind of accessible sample reflecting its features. In our study, we analyzed 45 sputum samples from 45 patients with CAP. Eighte...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmt116

    authors： Chen C,Shen T,Tian F,Lin P,Li Q,Cui Z,Zhang Y,Xue M,Ye J,Guo X,Zhou Y

    更新日期：2013-12-01 00:00:00

  • Edaravone injection reverses learning and memory deficits in a rat model of vascular dementia.

    abstract：:Edaravone is a novel free radical scavenger that exerts neuroprotective effects by inhibiting endothelial injury and by ameliorating neuronal damage in brain ischemia. Recently, it was reported that edaravone could alleviate the pathology and cognitive deficits of Alzheimer's disease patients. However, its relevance t...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmw116

    authors： Li X,Lu F,Li W,Qin L,Yao Y,Ge X,Yu Q,Liang X,Zhao D,Li X,Zhang J

    更新日期：2017-01-01 00:00:00

  • Ricin-B-lectin enhances microsporidia Nosema bombycis infection in BmN cells from silkworm Bombyx mori.

    abstract：:Nosema bombycis is an obligate intracellular parasitic fungus that utilizes a distinctive mechanism to infect Bombyx mori Spore germination can be used for host cell invasion; however, the detailed mechanism remains to be elucidated. The ricin-B-lectin (RBL) gene is significantly differentially regulated after N. bomb...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmw093

    authors： Liu H,Li M,Cai S,He X,Shao Y,Lu X

    更新日期：2016-11-01 00:00:00

  • Identification and functional characterization of a novel missense mutation in FRMD7 responsible for idiopathic congenital nystagmus.

    abstract：:Idiopathic congenital nystagmus (ICN) is a genetically heterogeneous eye movement disorder which seriously reduces childhood visual acuity. X-linked inheritance is the most common pattern, and mutations in FERM domain-containing protein 7 (FRMD7) are the major cause. Here, we recruited a four-generation Chinese family...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmy161

    authors： Wang Z,Wang M,Wang C,Lu B

    更新日期：2019-02-01 00:00:00

  • In vitro observation of the molecular interaction between NodD and its inducer naringenin as monitored by fluorescence resonance energy transfer.

    abstract：:At initial stages in the Rhizobium legume symbiosis, most nodulation genes are controlled by NodD protein and plant inducers. Some genetic studies and other reports have suggested that NodD may be activated by its direct interaction with plant inducers. However, there has been no molecular evidence of such an inducing...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：

    authors： Li F,Hou B,Chen L,Yao Z,Hong G

    更新日期：2008-09-01 00:00:00

  • Identification of differentially expressed genes in rat silicosis model by suppression subtractive hybridization analysis.

    abstract：:The critical molecular mechanism in the development of the pulmonary fibrosis remains unknown, leaving diagnosed patients with a poor prognosis. To isolate the genes specifically up-regulated in pulmonary fibrosis, we established a rat silicosis model 360 d after treatment with crystalline silica suspension. Radiograp...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：

    authors： Jin Z,Liu B,Feng D,Chen C,Li X,Hu Y,Peng J,Liu Y,Du J,Fu C,Wen J

    更新日期：2008-08-01 00:00:00

  • Differential expression of neutrophilic granule proteins between Th1 and Th2 cells.

    abstract：:T helper cell type 1 (Th1) and 2 (Th2) play central roles in immune regulation. To identify the novel genes differentially expressed between Th1 and Th2 cells, CD4+ T cells were isolated from DO11.10 transgenic mice and induced under Th1 or Th2 conditions. Microarray showed differential expression of neutrophilic gran...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2007.00242.x

    authors： Xie D,Liu Z,Li Z,Ji Y,Chen J,Sun B

    更新日期：2007-01-01 00:00:00

  • Intein-mediated rapid purification of recombinant human pituitary adenylate cyclase activating polypeptide.

    abstract：:In order to obtain the recombinant human PACAP efficiently by intein-mediated single column purification, a gene encoding human PACAP was synthesized and cloned into Escherichia coli expression vector pKYB. The recombinant vector pKY-PAC was transferred into E. coli ER2566 cells and the target protein was over-express...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/36.11.759

    authors： Yu RJ,Hong A,Dai Y,Gao Y

    更新日期：2004-11-01 00:00:00

  • Regulation of membrane band 3 Tyr-phosphorylation by proteolysis of p72(Syk) and possible involvement in senescence process.

    abstract：:Erythrocyte senescence is characterized by exposure of cell surface epitopes on cell membrane proteins leading to immune mediated removal of red blood cells. One mechanism for antigen formation is tyrosine phosphorylation (Tyr-P) of the transmembrane protein band 3 by Syk kinase. Our aim was to test the hypothesis tha...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmp071

    authors： Bordin L,Fiore C,Bragadin M,Brunati AM,Clari G

    更新日期：2009-10-01 00:00:00

  • Production of the polyclonal anti-human metallothionein 2A antibody with recombinant protein technology.

    abstract：:Metallothionein 2A (MT2A) is a small stress response protein that can be induced by exposure to toxic metals. It is highly expressed in breast cancer cells. In this study, the cDNA encoding the human MT2A protein was expressed as glutathione S-transferase (GST) fusion protein in Escherichia coli. Recombinant MT2A prot...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2006.00167.x

    authors： Marikar FM,Sun QM,Hua ZC

    更新日期：2006-05-01 00:00:00

  • SC1 inhibits the differentiation of F9 embryonic carcinoma cells induced by retinoic acid.

    abstract：:The ability to self-renew is one of the most important properties of embryonic stem (ES) cells. Pluripotin (SC1), a small molecule with high activity and low toxicity, promotes self-renewal in mouse ES cells. SC1 can noticeably change the morphology of retinoic acid (RA)-induced F9 embryonic carcinoma cells (F9 cells)...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmy069

    authors： Liu Y,Ren X,Ke J,Zhang Y,Wei Q,Shi Z,Ai Z,Guo Z

    更新日期：2018-08-01 00:00:00

  • New member of the guanosine triphosphatase activating protein family in the human epididymis.

    abstract：:The effect of the guanosine triphosphatase activating proteins (GAPs) on spermatogenesis has been studied for years, though no GAPs have been explored in epididymis, an essential organ for sperm maturation. In this study, a new GAP member, designated as MacGAP, was cloned in human epididymis. The MacGAP gene encodes a...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：

    authors： Li X,Liu Q,Liu S,Zhang J,Zhang Y

    更新日期：2008-10-01 00:00:00

  • Post-transcriptional regulation of NifA expression by Hfq and RNase E complex in Rhizobium leguminosarum bv. viciae.

    abstract：:NifA is the general transcriptional activator of nitrogen fixation genes in diazotrophic bacteria. In Rhizobium leguminosarum bv. viciae strain 8401/pRL1JI, the NifA gene is part of a gene cluster (fixABCXNifAB). In this study, results showed that in R. leguminosarum bv.viciae 8401/pRL1JI, host factor required (Hfq), ...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmp060

    authors： Zhang Y,Hong G

    更新日期：2009-09-01 00:00:00

  • miR-375 is highly expressed and possibly transactivated by achaete-scute complex homolog 1 in small-cell lung cancer cells.

    abstract：:In this study, we identified five miRNAs highly expressed in the small-cell lung cancer (SCLC) cell line NCI-H209. Among them, the expression levels of miR-375 were dramatically elevated in all SCLC cell lines examined, coincident with the expression of the transcription factor achaete-scute complex homolog 1 (ASCL1)....

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmr110

    authors： Zhao H,Zhu L,Jin Y,Ji H,Yan X,Zhu X

    更新日期：2012-02-01 00:00:00

  • The structure-function relationship of MSI7, a matrix protein from pearl oyster Pinctada fucata.

    abstract：:We previously identified a matrix protein, MSI7, from pearl oyster Pinctada fucata. According to the structural analysis, the DGD site in the N-terminal of MSI7 is crucial for its role in the shell formation. In this study, we expressed a series of recombinant MSI7 proteins, including the wild-type and several mutants...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmp086

    authors： Feng Q,Fang Z,Yan Z,Xing R,Xie L,Zhang R

    更新日期：2009-11-01 00:00:00

  • The regulation and function of YAP transcription co-activator.

    abstract：:The Hippo pathway was initially identified in Drosophila by genetic mosaic screens for tumor suppressor genes. Researches indicated that the Hippo pathway is a key regulator of organ size and is conserved during evolution. Furthermore, studies of mouse models and clinical samples demonstrated the importance of Hippo p...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章,评审

    doi：10.1093/abbs/gmu110

    authors： Zhu C,Li L,Zhao B

    更新日期：2015-01-01 00:00:00

  • The influence of hyaluronic acid on vascular endothelial cell proliferation and the relationship with ezrin/merlin expression.

    abstract：:It has been established that hyaluronic acid (HA) glycans (nHA) and oligosaccharide (oHA) exert different effects on the biological function of the vascular endothelial cell (EC), resulting in altered regulation of angiogenesis. However, the specific mechanism is still unclear. Our study focused on the effects of nHA ...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmr094

    authors： Mo W,Yang C,Liu Y,He Y,Wang Y,Gao F

    更新日期：2011-12-01 00:00:00

  • MicroRNA-193b modulates proliferation, migration, and invasion of non-small cell lung cancer cells.

    abstract：:MicroRNAs have been reported to be closely related to the development of human lung cancers. However, the functions of microRNAs in non-small cell lung cancer (NSCLC) remain largely undefined. Here, we investigated the role of microRNA-193b (miR-193b) in NSCLC. Our data showed that miR-193b was markedly down-regulated...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gms018

    authors： Hu H,Li S,Liu J,Ni B

    更新日期：2012-05-01 00:00:00

  • The Mycobacterium bovis BCG prime-Rv0577 DNA boost vaccination induces a durable Th1 immune response in mice.

    abstract：:Tuberculosis remains a major global health problem and effective vaccines are urgently needed. In this study, we used the combined DNA- and protein-based vaccines of immunodominant antigen Rv0577 to boost BCG and evaluated their immunogenicity in BALB/c mice. Our data suggest that the booster vaccine may substantially...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1093/abbs/gmw010

    authors： Gu D,Chen W,Mi Y,Gong X,Luo T,Bao L

    更新日期：2016-04-01 00:00:00

  • Purification and partial characterization of beta-glucosidase from fresh leaves of tea plants (Camellia sinensis (L.) O. Kuntze).

    abstract：:beta-Glucosidases are important in the formation of floral tea aroma and the development of resistance to pathogens and herbivores in tea plants. A novel beta-glucosidase was purified 117-fold to homogeneity, with a yield of 1.26%, from tea leaves by chilled acetone and ammonium sulfate precipitation, ion exchange chr...

    journal_title：Acta biochimica et biophysica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7270.2005.00053.x

    authors： Li YY,Jiang CJ,Wan XC,Zhang ZZ,Li DX

    更新日期：2005-06-01 00:00:00