Biotinyl C-terminal-extended motilin as a biologically active receptor probe.


:The synthesis, purification, and characterization of biotinylated analogues of motilin are reported. The C-terminal of canine motilin was extended by the addition of a cysteine residue, and then biotinylated. Biotinyl motilin was purified by following HPLC and characterized by amino acid analysis. Biotinylation of the ligand was confirmed by ELISA assay with the avidin-biotin system. Biotinyl motilin showed similar affinity for binding to rabbit gastric membrane fraction compared to unlabeled canine motilin, and also retained functional activity in its ability to cause contraction of rabbit duodenal segments. To determine the binding of biotinyl motilin in isolated rabbit antral smooth muscle, cells were incubated with the biotinyl motilin with and without excess of unlabeled motilin. Subsequent addition of avidin-biotinylated peroxidase complex showed the distribution of reaction products over the cell surface. Bioactive biotinyl motilin provides a useful probe for the demonstration of cell surface motilin receptors and will facilitate receptor purification and characterization.






Sakai T,Satoh M,Hayashi H,Fujikura K,Sano I,Koyama H,Tatemoto K,Itoh Z




Has Abstract


1994-01-01 00:00:00
















  • Des-Arg9-bradykinin metabolism in patients who presented hypersensitivity reactions during hemodialysis: role of serum ACE and aminopeptidase P.

    abstract::Bradykinin (BK) has been proposed as the principal mediator of hypersensitivity reactions (HSR) in patients dialyzed using negatively charged membranes and concomitantly treated with angiotensin-converting enzyme (ACE) inhibitors. We investigated the metabolism of exogenous BK added to the sera of 13 patients dialyzed...


    pub_type: 杂志文章


    authors: Blais C Jr,Marc-Aurèle J,Simmons WH,Loute G,Thibault P,Skidgel RA,Adam A

    更新日期:1999-01-01 00:00:00

  • Effect of DN-1417, a thyrotropin releasing hormone analog, on dopaminergic neurons in rat brain.

    abstract::Acute and chronic effects of gamma-butyrolactone-gamma-carbonyl-histidyl-prolinamide (DN-1417) were investigated on motor activity, dopamine (DA) metabolites and DA receptors in various brain regions of rats. The motor activity, as measured with Automex recorder, was enhanced after a single injection with DN-1417 (20 ...


    pub_type: 杂志文章


    authors: Nakahara T,Matsumoto T,Hirano M,Uchimura H,Yokoo H,Nakamura K,Ishibashi K,Hirano H

    更新日期:1985-11-01 00:00:00

  • Motilin and a synthetic enkephalin induce colonic motor complexes (CMC) in the conscious dog.

    abstract::In the conscious dog migrating and nonmigrating colonic motor complexes (CMC) were recorded by means of chronically implanted strain gauge force transducers. Intravenous injection of a synthetic enkephalin analogue immediately induced a premature CMC at all three recording sites of the colon. Naloxone inhibited the en...


    pub_type: 杂志文章


    authors: Bickel M,Belz U

    更新日期:1988-05-01 00:00:00

  • Synthetic peptides derived from human antimicrobial peptide ubiquicidin accumulate at sites of infections and eradicate (multi-drug resistant) Staphylococcus aureus in mice.

    abstract::The presence and antimicrobial activity of antimicrobial peptides (AMPs) has been widely recognized as an evolutionary preserved part of the innate immune system. Based on evidence in animal models and humans, AMPs are now positioned as novel anti-infective agents. The current study aimed to evaluate the potential ant...


    pub_type: 杂志文章


    authors: Brouwer CP,Bogaards SJ,Wulferink M,Velders MP,Welling MM

    更新日期:2006-11-01 00:00:00

  • Multiple Y receptors mediate pancreatic polypeptide responses in mouse colon mucosa.

    abstract::A functional study has been performed to characterise the Y receptors responsible for NPY, PYY and PP-stimulated responses in mouse colonic mucosal preparations. Electrogenic ion secretion was stimulated with VIP following which NPY, PYY and PP analogues were, to varying degrees, inhibitory. PYY(3-36), hPP, Gln(23)hPP...


    pub_type: 杂志文章


    authors: Cox HM,Pollock EL,Tough IR,Herzog H

    更新日期:2001-03-01 00:00:00

  • Binding of Bacillus thuringiensis subsp. israelensis Cry4Ba to Cyt1Aa has an important role in synergism.

    abstract::Bacillus thuringiensis subsp. israelensis (Bti) produces at least four different crystal proteins that are specifically toxic to different mosquito species and that belong to two non-related family of toxins, Cry and Cyt named Cry4Aa, Cry4Ba, Cry11Aa and Cyt1Aa. Cyt1Aa enhances the activity of Cry4Aa, Cry4Ba or Cry11A...


    pub_type: 杂志文章


    authors: Cantón PE,Zanicthe Reyes EZ,Ruiz de Escudero I,Bravo A,Soberón M

    更新日期:2011-03-01 00:00:00

  • Antimicrobial peptide control of pathogenic microorganisms of the oral cavity: a review of the literature.

    abstract::Antimicrobial peptides, molecules produced in many different organisms, have high biocidal activity against several microorganisms. However, several questions about these molecules remain unclear. Therefore, this report details a systematic survey of the literature on the use of antimicrobial peptides against oral pat...


    pub_type: 杂志文章,评审


    authors: da Silva BR,de Freitas VA,Nascimento-Neto LG,Carneiro VA,Arruda FV,de Aguiar AS,Cavada BS,Teixeira EH

    更新日期:2012-08-01 00:00:00

  • Gonadotropin-releasing hormone neuropeptides and receptor in human breast cancer: correlation to poor prognosis parameters.

    abstract::Expression of the two gonadotropin-releasing hormone homologue peptides GnRHI and GnRHII and their receptor GnRHR has been demonstrated in a number of malignancies. In hormone-dependent breast cancer, GnRH analogs are used for therapy in premenopausal women. Gene expression of GnRHI, II and R was studied in breast bio...


    pub_type: 杂志文章


    authors: Pazaitou-Panayiotou K,Chemonidou C,Poupi A,Koureta M,Kaprara A,Lambropoulou M,Constantinidis TC,Galaktidou G,Koffa M,Kiziridou A,Kakolyris S,Kolios G,Kortsaris A,Chatzaki E

    更新日期:2013-04-01 00:00:00

  • Tumor necrosis factor-alpha downregulates adrenomedullin receptors in human coronary artery smooth muscle cells.

    abstract::We examined the effects of tumor necrosis factor (TNF)-alpha on the expression and functionality of adrenomedullin (AM) receptors in cultured human coronary artery smooth muscle cells. Analysis of real-time quantitative polymerase chain reactions showed that these cells abundantly express two AM receptors comprised of...


    pub_type: 杂志文章


    authors: Nagoshi Y,Kuwasako K,Cao YN,Imamura T,Kitamura K,Eto T

    更新日期:2004-07-01 00:00:00

  • Pc16a, the first characterized peptide from Conus pictus venom, shows a novel disulfide connectivity.

    abstract::A novel conotoxin, pc16a, was isolated from the venom of Conus pictus. This is the first peptide characterized from this South-African cone snail and it has only 11 amino acid residues, SCSCKRNFLCC*, with the rare cysteine framework XVI and a monoisotopic mass of 1257.6Da. Two peptides were synthesized with two possib...


    pub_type: 杂志文章


    authors: Van Der Haegen A,Peigneur S,Dyubankova N,Möller C,Marí F,Diego-García E,Naudé R,Lescrinier E,Herdewijn P,Tytgat J

    更新日期:2012-03-01 00:00:00

  • Possible mechanisms of positive inotropic action of synthetic human calcitonin gene-related peptide in isolated rat atrium.

    abstract::The effect of synthetic human calcitonin gene-related peptide (hCGRP) on the isolated and electrically driven left atria of rats were investigated. The peptide at concentrations of 3 X 10(-9)-3 X 10(-7) M produced positive inotropic effects on the left atria in a dose-dependent manner. Verapamil (10(-5) M) and adenosi...


    pub_type: 杂志文章


    authors: Satoh M,Oku R,Maeda A,Fujii N,Otaka A,Funakoshi S,Yajima H,Takagi H

    更新日期:1986-07-01 00:00:00

  • Adrenomedullin binds with high affinity, elevates cyclic AMP, and stimulates c-fos mRNA in C6 glioma cells.

    abstract::The effects of adrenomedullin (ADM) on C6 glioma cells were investigated. [125I]ADM bound with high affinity (Kd = 24 nM) to a single class of sites (Bmax = 36,000/cell) in C6 cells. Specific [125I]ADM binding was inhibited with high affinity by ADM (IC50 value of 10 nM) but not ADM(22-52) or pro-adrenomedullin N-term...


    pub_type: 杂志文章


    authors: Moody TW,Miller MJ,Mártinez A,Unsworth E,Cuttitta F

    更新日期:1997-01-01 00:00:00

  • Circadian oscillations of RPCH gene expression in the eyestalk of the crayfish Cherax quadricarinatus.

    abstract::The RPCH and beta-actin cDNAs from the crayfish Cherax quadricarinatus were amplified, cloned and sequenced. The primary structure sequences of these cDNAs were compared to other members of the AKH/RPCH family. Fluctuations in the amount of the C. quadricarinatus RPCH and beta-actin mRNAs, as cDNAs, were quantified ev...


    pub_type: 杂志文章


    authors: Martínez-Pérez F,Zinker S,Aguilar G,Valdés J,Aréchiga H

    更新日期:2005-12-01 00:00:00

  • Arginine vasopressin facilitates reversal learning in albino, but not hooded rats.

    abstract::Male Holtzman albino and Long-Evans hooded rats were administered one microgram of arginine vasopressin (AVP) or a placebo each day after the acquisition trials of a visual white-black discrimination. Animals were then trained in the reversal of the discrimination. Performance was assessed by the number of trials to c...


    pub_type: 杂志文章


    authors: Tinius TP,Beckwith BE,Oltmanns D

    更新日期:1989-01-01 00:00:00

  • Tissue distribution and plasma clearance of heparin-binding EGF-like growth factor (HB-EGF) in adult and newborn rats.

    abstract::Heparin-binding EGF-like growth factor (HB-EGF), a member of the epidermal growth factor (EGF) family, can protect intestinal epithelial cells from various forms of injury in vitro and attenuate intestinal ischemia/reperfusion damage in vivo. With the goal of eventual clinical use of HB-EGF to protect the intestines f...


    pub_type: 杂志文章


    authors: Feng J,Mehta VB,El-Assal ON,Wu D,Besner GE

    更新日期:2006-06-01 00:00:00

  • Cholecystokinin octa- and tetrapeptide degradation by synaptic membranes. III. Inactivation of CCK-8 by a phosphoramidon-sensitive endopeptidase.

    abstract::Solubilization of rat synaptic membranes by Triton X-100 followed by DEAE-cellulose chromatography allowed the separation of a phosphoramidon-sensitive endopeptidase that cleaved CCK-8. This enzymatic activity revealed similar if not identical to "enkephalinase A." A major cleavage point, at the Trp30-Met31 bond, and ...


    pub_type: 杂志文章


    authors: Deschodt-Lanckman M,Koulischer D,Przedborski S,Lauwereys M

    更新日期:1984-05-01 00:00:00

  • Metabolic effects of chronic obestatin infusion in rats.

    abstract::Obestatin is purported to be a peptide hormone encoded in preproghrelin. We studied the metabolic effects of continuous infusion of obestatin via subcutaneously implanted osmotic mini-pumps. Administration of up to 500nmol/kg body weight/day obestatin did not change 24h cumulative food intake or body weight in rats. S...


    pub_type: 杂志文章


    authors: Unniappan S,Speck M,Kieffer TJ

    更新日期:2008-08-01 00:00:00

  • Agrocybin, an antifungal peptide from the edible mushroom Agrocybe cylindracea.

    abstract::An antifungal peptide with a molecular mass of 9 kDa was isolated from fresh fruiting bodies of the mushroom Agrocybe cylindracea. The isolation procedure comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography by fast protein liquid chromatogr...


    pub_type: 杂志文章


    authors: Ngai PH,Zhao Z,Ng TB

    更新日期:2005-02-01 00:00:00

  • Interaction of antimicrobial peptides with bacterial polysaccharides from lung pathogens.

    abstract::The interaction of two cathelicidin antimicrobial peptides, LL-37 and SMAP-29, with three bacterial polysaccharides, respectively, produced by Pseudomonas aeruginosa, Burkholderia cepacia and Klebsiella pneumoniae, was investigated to identify possible mechanisms adopted by lung pathogens to escape the action of innat...


    pub_type: 杂志文章


    authors: Herasimenka Y,Benincasa M,Mattiuzzo M,Cescutti P,Gennaro R,Rizzo R

    更新日期:2005-07-01 00:00:00

  • Anti-tumor activities of peptides corresponding to conserved complementary determining regions from different immunoglobulins.

    abstract::Short synthetic peptides corresponding to sequences of complementarity-determining regions (CDRs) from different immunoglobulin families have been shown to induce antimicrobial, antiviral and antitumor activities regardless of the specificity of the original monoclonal antibody (mAb). Presently, we studied the in vitr...


    pub_type: 杂志文章


    authors: Figueiredo CR,Matsuo AL,Massaoka MH,Polonelli L,Travassos LR

    更新日期:2014-09-01 00:00:00

  • Pharmacology of stomoxytachykinin receptor depends on second messenger system.

    abstract::STKR is a neurokinin receptor derived from the stable fly, Stomoxys calcitrans. Insect tachykinin-related peptides, also referred to as "insectatachykinins", produce dose-dependent calcium and cyclic AMP responses in cultured Drosophila melanogaster Schneider 2 (S2) cells that were stably transfected with the cloned S...


    pub_type: 杂志文章


    authors: Poels J,Nachman RJ,Akerman KE,Oonk HB,Guerrero F,De Loof A,Janecka AE,Torfs H,Vanden Broeck J

    更新日期:2005-01-01 00:00:00

  • Four cross-linked HIV Gag peptides prime the immune response to HIV proteins in mice.

    abstract::The functional help provided by four cross-linked synthetic peptides from HIV-1 Gag structural proteins was investigated in the mouse model. These peptides, selected upon non-self-criteria, are not predicted as T epitopes by classical prediction methods such as the Rothbard consensus or the amphipathy rule. Priming mi...


    pub_type: 杂志文章


    authors: Benveniste O,Vaslin B,Dormont D

    更新日期:1994-01-01 00:00:00

  • The antibacterial peptide ceratotoxin A displays alamethicin-like behavior in lipid bilayers.

    abstract::Ceratotoxin A (CtxA), a 36-residue alpha-helical cationic peptide isolated from the medfly Ceratitis capitata, exhibits strong antibacterial activity. To determine its mode of action against bacteria, we investigated the behavior of ceratotoxin A by incorporating it into planar lipid bilayers. Macroscopic and single c...


    pub_type: 杂志文章


    authors: Saint N,Marri L,Marchini D,Molle G

    更新日期:2003-11-01 00:00:00

  • Visualization of neuropeptides in paraffin-embedded tissue sections of the central nervous system in the decapod crustacean, Penaeus monodon, by imaging mass spectrometry.

    abstract::The distributions of neuropeptides in paraffin-embedded tissue sections (PETS) of the eyestalk, brain, and thoracic ganglia of the shrimp Penaeus monodon were visualized by imaging mass spectrometry (IMS). Peptide signals were obtained from PETS without affecting morphological features. Twenty-nine neuropeptides compr...


    pub_type: 杂志文章


    authors: Chansela P,Goto-Inoue N,Zaima N,Sroyraya M,Sobhon P,Setou M

    更新日期:2012-03-01 00:00:00

  • In vivo administration of the frog skin peptide frenatin 2.1S induces immunostimulatory phenotypes of mouse mononuclear cells.

    abstract::Host-defense peptides secreted by epithelial cells exhibit cytotoxic and immunoregulatory effects in order to protect the organism against invading microorganisms. Antimicrobial peptides derived from frog skin display both immunostimulatory and immunosuppressive actions as demonstrated by in vitro cytokine production ...


    pub_type: 杂志文章


    authors: Pantic JM,Radosavljevic GD,Jovanovic IP,Arsenijevic NN,Conlon JM,Lukic ML

    更新日期:2015-09-01 00:00:00

  • Influence of altitude and caffeine during rest and exercise on plasma levels of proenkephalin peptide F.

    abstract::The purpose of this study was to examine the resting and exercise response patterns of plasma Peptide F immunoreactivity (ir) to altitude exposure (4300 m) and caffeine ingestion (4 b.w.-1). Nine healthy male subjects performed exercise tests to exhaustion (80-85% VO2max) at sea level (50 m), during an acute alt...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Kraemer WJ,Rock PB,Fulco CS,Gordon SE,Bonner JP,Cruthirds CD,Marchitelli LJ,Trad L,Cymerman A

    更新日期:1988-09-01 00:00:00

  • MCH and feeding behavior-interaction with peptidic network.

    abstract::Numerous works associate the MCH peptide, and the hypothalamic neurons that produce it, to the feeding behavior and energy homeostasis. It is commonly admitted that MCH is an orexigenic peptide, and MCH neurons could be under the control of arcuate NPY and POMC neurons. However, the literature data is not always conco...


    pub_type: 杂志文章,评审


    authors: Griffond B,Risold PY

    更新日期:2009-11-01 00:00:00

  • Unexpected high density of neuropeptide Y Y1 receptors in the guinea pig spleen.

    abstract::Receptors for neuropeptide Y (NPY) and peptide YY (PYY) have been extensively characterized in the brain. Less is known about NPY receptor subtypes in the spleen, though it is well established that NPY produces vascular contraction in this tissue. In the present study, we found an unusually high density of Y1 receptor...


    pub_type: 杂志文章


    authors: Gehlert DR,Gackenheimer SL

    更新日期:1996-01-01 00:00:00

  • Analogs of RGDVY and GRGD peptides inhibit Mycobacterium kansasii phagocytosis.

    abstract::Continuing our research on Mycobacteria kansasii phagocytosis inhibition, we have examined in that context three series of peptides derived from the RGDVY and GRGD sequences. It was found that the levels of the inhibitory activity depend on the amino acid composition as well as on the particular peptide sequence. Dist...


    pub_type: 杂志文章


    authors: Siemion IZ,Gawlowska M,Krajewski K,Strug I,Wieczorek Z

    更新日期:2003-08-01 00:00:00

  • Change in substance P in a firearm wound and its significance.

    abstract::The present study was designed to detect the change of substance P (SP) in firearm wounds and its relationship with wound healing. Twenty two rabbits were randomly divided into two groups, one with firearm wounds created by steel balls shooting rabbits' thighs and another with stab wounds created by knife. The experim...


    pub_type: 杂志文章


    authors: Xinan L,Suguang L,Liangchao Z,Lei C

    更新日期:1998-01-01 00:00:00