Effect of age and disease on two drug binding proteins: albumin and alpha-1- acid glycoprotein.

abstract：OBJECTIVES:To verify the role of IL-12 in the pathogenesis of psoriasis and determine its relation to IFNgamma. DESIGN AND METHODS:Skin biopsies from lesional and non-lesional skin of 30 patients and 10 healthy controls were obtained for quantitative PCR examination of IL-12 (P40) and IFNgamma mRNA as well as in situ ...

journal_title：Clinical biochemistry

authors： Shaker OG,Moustafa W,Essmat S,Abdel-Halim M,El-Komy M

更新日期：2006-02-01 00:00:00

abstract：:57Co-cyanocobalamin is substituted for iodine labeled antigen in three commercially available solid phase radioimmunoassay kits to evaluate the extent of non-specific binding by determination of the supernatant and residual fraction of unbound ligand and thus obtain a value for corrected binding throughout the range o...

journal_title：Clinical biochemistry

authors： Carter P

更新日期：1978-06-01 00:00:00

abstract：OBJECTIVES:To investigate whether the STK15 mRNA expression correlates with clinicopathologic features and the prognosis of HCC patients. DESIGN AND METHODS:Three hepatoma cell lines, two normal liver epithelial cell lines, hepatoma tissues, adjacent tumor tissues and normal liver tissues were obtained from 46 HCC pat...

journal_title：Clinical biochemistry

authors： Wang R,Wang JH,Chu XY,Geng HC,Chen LB

更新日期：2009-05-01 00:00:00

abstract：:To elucidate the diagnostic relevance of biliary conjugated bilirubin, biliary bilirubin from normal volunteers (NV), patients with Gilbert's syndrome (GS) and Crigler-Najjar syndrome type II (C-N II), and from various rat strains was fractionated. Biliary bilirubin diglucuronide (BDG) was present at lower levels, and...

journal_title：Clinical biochemistry

authors： Adachi Y,Yamashita M,Nanno T,Yamamoto T

更新日期：1990-04-01 00:00:00

abstract：:1. An automated method is described for the assay of pregnancy serum cystine aminopeptidase (Oxytocinase, E.C. 3.4.1.10) using continuous flow analysis. S-Genzyl-L-cysteine-p-nitroanilide, the most highly specific substrate so far available for oxytocinase is employed in this assay. Comparison with the manual method f...

journal_title：Clinical biochemistry

authors： Small CW,Watkins WB

更新日期：1975-04-01 00:00:00

abstract：OBJECTIVES:The aim of the study was to evaluate the concentration of interleukin 12 (IL-12), the activity of phospholipase A(2) (PLA(2)), and platelet-activating factor acetylhydrolase (PAF-AH) in type 1 diabetes (DM1) patients treated with intensive insulin therapy. DESIGN AND METHODS:Studied parameters were measured...

journal_title：Clinical biochemistry

authors： Wegner M,Araszkiewicz A,Pioruńska-Mikołajczak A,Zozulińska-Ziółkiewicz D,Wierusz-Wysocka B,Pioruńska-Stolzmann M

更新日期：2009-11-01 00:00:00

abstract：:Congestive heart failure usually features a mild to moderate increase in aspartate aminotransferase (AST) activity. A marked increase in AST may occur rarely and is usually preceded by prolonged shock or hypotensive episodes and is accompanied by hyperbilirubinemia. I report two patients with congestive heart failure ...

journal_title：Clinical biochemistry

authors： Nanji AA

更新日期：1983-04-01 00:00:00

abstract：:The use of point-of-care (POC) immunoassays has increased significantly and the menu of analytes continues to expand. Most of the rapid immunoassays are currently based on simple manual assay devices such as the immunochromatographic, agglutination, and immunofiltration assays. Although automated readers have recently...

journal_title：Clinical biochemistry

authors： von Lode P

更新日期：2005-07-01 00:00:00

abstract：OBJECTIVES:To examine the diagnostic validity of bone alkaline phosphatase (BAP) in serum (a marker of bone formation) and of urinary excretion of pyridinium cross-links (PYR) (a marker of bone resorption) for detection of bone metastases. DESIGN AND METHODS:Two heterogeneous immunoassays were employed for determinati...

journal_title：Clinical biochemistry

authors： Withold W,Georgescu G,Khakzad H,Vosberg H,Mueller-Gaertner HW,Reinauer H

更新日期：1995-10-01 00:00:00

abstract：OBJECTIVE:To confirm that the naproxen metabolite O-desmethylnaproxen interferes with the Jendrassik and Grof method for total bilirubin and was a possible cause of spurious hyperbilirubinemia in a case of naproxen overdose. DESIGN AND METHODS:Plasma was spiked with naproxen or O-desmethylnaproxen before total and dir...

journal_title：Clinical biochemistry

authors： Al Riyami N,Zimmerman AC,Rosenberg FM,Holmes DT

更新日期：2009-01-01 00:00:00

abstract：OBJECTIVES:The change in DNA methylation patterns can be used to distinguish between normal and cancer cells. The aim of the present study was to examine the 5' CpG island methylation patterns of the cancer-testis antigen (CT antigen) gene family, MAGE-As, in hepatocellular carcinoma (HCC), and to develop the DNA demet...

journal_title：Clinical biochemistry

authors： Qiu G,Fang J,He Y

更新日期：2006-03-01 00:00:00

abstract：OBJECTIVES:An increased level of serum vascular adhesion protein-1 (VAP-1) has been found in patients with diabetes mellitus and vascular disorders. This study examined whether serum VAP-1 levels are associated with chronic kidney disease (CKD). DESIGN AND METHODS:We included 262 subjects aged 30 and above with fastin...

journal_title：Clinical biochemistry

authors： Lin MS,Li HY,Wei JN,Lin CH,Smith DJ,Vainio J,Shih SR,Chen YH,Lin LC,Kao HL,Chuang LM,Chen MF

更新日期：2008-11-01 00:00:00

abstract：OBJECTIVES:Studies from both experimental animals and humans suggest that administration of exogenous DHEA may have beneficial endocrine-metabolic, immunologic and neurologic effects. Several groups have administered DHEA to humans, but to the best of our knowledge, no one at this point has published a summary of the r...

journal_title：Clinical biochemistry

authors： Tummala S,Svec F

更新日期：1999-07-01 00:00:00

abstract：:1. A CK(BB) fraction was observed in sera of a patient with prostatic carcinoma. The fraction accounted for 59% of the total enzyme activity. A metastatic tumor of a prostatic carcinoma was observed to contain solely the CK(BB) isozyme. An embryonal testicular carcinoma was observed to have a higher percentage of the ...

journal_title：Clinical biochemistry

authors： Hoag G,Amies DR,Colquhoun BD

更新日期：1978-04-01 00:00:00

abstract：OBJECTIVES:The aim of the study was to examine the long-term effect of commonly used whole soy foods (soy flour) and purified daidzein (one major isoflavone and the precursor of equol) on renal function among prehypertensive postmenopausal women who are also equol producers, a population most likely to benefit from soy...

journal_title：Clinical biochemistry

authors： Liu ZM,Ho SC,Chen YM,Tang N,Woo J

更新日期：2014-09-01 00:00:00

abstract：OBJECTIVES:Recurrent miscarriage (RM) is a challenging medical problem because of its unknown pathogenesis and etiology in most of the cases. Recent studies suggest the role of persistent environmental pollutants such as organochlorine pesticides (OCPs) in the etiology of RM. The present study was conducted to investig...

journal_title：Clinical biochemistry

authors： Pathak R,Mustafa M,Ahmed RS,Tripathi AK,Guleria K,Banerjee BD

更新日期：2010-01-01 00:00:00

abstract：OBJECTIVE:To assess the generation of reactive oxygen species (ROS) and the involvement of the main antioxidant pathways in low/intermediate-1-risk myelodysplastic syndromes (MDS) with iron overload (IOL). METHODS:We examined the levels of superoxide anion (O2-), hydrogen peroxide (H2O2), antioxidants (glutathione, GS...

journal_title：Clinical biochemistry

authors： Ivars D,Orero MT,Javier K,Díaz-Vico L,García-Giménez JL,Mena S,Tormos C,Egea M,Pérez PL,Arrizabalaga B,Ruiz MÁ,Yagüe N,Tormo M,Sancho-Tello R,Gomes A,Algueró C,O'Connor JE,Sáez GT,Carbonell F,Collado R

更新日期：2017-11-01 00:00:00

abstract：:We describe a simple direct extraction method for the gas-liquid chromatography determination of serum valproic acid. The working range for the assay is 2-180 mg/L and our within-run precision was 5.8 and 4.3% at the 40 and 90 mg/L concentrations respectively. Hemolyzed and lipemic sera as well as samples from patient...

journal_title：Clinical biochemistry

authors： Leroux M,Budnik D,Hall K,Irvine-Meek J,Otten N,Seshia S

更新日期：1981-04-01 00:00:00

abstract：:The purpose of this report is to evaluate whether a new, simple, non-invasive method for chymotrypsin measurement in stools is useful for the diagnosis of exocrine pancreatic insufficiency in cystic fibrosis (CF). A hundred children aged from 2 months to 12 years were tested: 50 children had been admitted for chronic ...

journal_title：Clinical biochemistry

authors： Scotta MS,Marzani MD,Maggiore G,De Giacomo C,Melzi D'Eril GV,Moratti R

更新日期：1985-08-01 00:00:00

abstract：:Matrix metalloproteinases (MMPs) may play a pathophysiological role in the development of diabetic nephropathy (DN). We hypothesized that urinary MMP activity in patients with type 2 diabetes mellitus (T2DM) is related to a decline in renal function. We determined MMP-2, -8 and -9 activity in 24-h urine collections in...

journal_title：Clinical biochemistry

authors： van der Zijl NJ,Hanemaaijer R,Tushuizen ME,Schindhelm RK,Boerop J,Rustemeijer C,Bilo HJ,Verheijen JH,Diamant M

更新日期：2010-05-01 00:00:00

abstract：OBJECTIVES:Coenzyme Q(10) (CoQ(10)) is an essential electron carrier in the mitochondrial respiratory chain and a strong antioxidant. Signs associated with skin alteration and mitochondrial dysfunction have been observed in patients with fibromyalgia (FM). The aim of this study was to analyze CoQ(10) levels, mitochondr...

journal_title：Clinical biochemistry

authors： Cordero MD,Moreno-Fernández AM,Carmona-López MI,Sánchez-Alcázar JA,Rodríguez AF,Navas P,de Miguel M

更新日期：2010-09-01 00:00:00

abstract：:We assessed the diagnostic value of four commercially available methods for determining pancreatic lipase (LPS) in serum (the turbidimetric procedure from Boehringer, two enzymatic approaches from Kodak and Poli, and an immunochemical assay) in a population of 46 hospitalized patients with acute abdominal pain. In 31 ...

journal_title：Clinical biochemistry

authors： Panteghini M,Pagani F,Bonora R,Alebardi O,Ceriotti F

更新日期：1991-12-01 00:00:00

abstract：OBJECTIVES:Despite the evidence that both homocysteine and cysteine are important risk factors for vascular disease and atherosclerosis no information are reported about their effective amount in plaque and on the relationship with the other low molecular weight thiols. DESIGN AND METHODS:We used capillary electrophor...

journal_title：Clinical biochemistry

authors： Zinellu A,Lepedda A Jr,Sotgia S,Zinellu E,Scanu B,Turrini F,Spirito R,Deiana L,Formato M,Carru C

更新日期：2009-06-01 00:00:00

abstract：OBJECTIVES:The relationship between glycated hemoglobin A1c (HbA1c) and average glucose has been described by the empirically derived estimated average glucose (eAG) equation in the A1c-Derived Average Glucose (ADAG) study, with extensive calibration efforts in four secondary reference HbA1c methods. It is not known if...

journal_title：Clinical biochemistry

authors： Loh TP,Sethi SK,Wong MS,Tai ES,Kao SL

更新日期：2015-05-01 00:00:00

abstract：:The measurement of serum 25-hydroxycalciferol (25-OH D) has proved to be a reliable index of the vitamin D nutritional status in man providing the renal 25-hydroxycalciferol-1 alpha-hydroxylase is functional. Since it is known that 25 OHD binds to alpha-globulins for its transport to the kidney, we have developed a co...

journal_title：Clinical biochemistry

authors： Delvin EE,Dussault M,Glorieux FH

更新日期：1980-06-01 00:00:00

abstract：UNLABELLED:Antioxidant capacity testing is commonly used in clinical investigations to provide an estimate of in vitro antioxidant capacity of biosamples. Although beneficial to measure the synergistic contribution of all compounds with antioxidant functionality, assessing the capacity of non-protein fractions or small...

journal_title：Clinical biochemistry

authors： Crowe KM

更新日期：2014-10-01 00:00:00

abstract：:Plasma membrane-bound 5'-nucleotidase (5'-NT), gamma-glutamyltransferase (gamma-GT) and soluble deoxynucleotidyltransferase (TdT) were studied in peripheral blood cells (PBMN) of 35 individuals, 26 male and 9 female, with circulating anti-HIV antibodies. Twenty-six were drug abusers, 2 were drug abusers and homosexual...

journal_title：Clinical biochemistry

authors： Losa GA

更新日期：1989-08-01 00:00:00

abstract：:To ensure effective antimicrobial or tumouricidal function, phagocytic cells must maintain their cytoplasmic pH (pHi) at a level conductive to optimal intracellular enzyme activity. The mechanisms by which neutrophils and macrophages regulate their cytoplasmic pH include bicarbonate-independent ion transport systems, ...

journal_title：Clinical biochemistry

authors： Grinstein S,Swallow CJ,Rotstein OD

更新日期：1991-06-01 00:00:00

abstract：OBJECTIVE:The aim of this study was to find a pre-interventional marker with the capacity to predict in-stent restenosis (ISR). Considering the anti-atherosclerotic role of adiponectin (APO), an adipocytokine with anti-inflammatory, anti-proliferative, anti-oxidative and anti-thrombotic properties, low plasma levels of...

journal_title：Clinical biochemistry

authors： Moldoveanu E,Mut-Vitcu B,Tanaseanu GR,Marta DS,Manea G,Kosaka T,Vidulescu C,Tanaseanu C

更新日期：2008-12-01 00:00:00

abstract：OBJECTIVES:Recently it has been suggested that serum beta-N-acetylhexosaminidase (Hex) could be a joint destruction marker in rheumatoid arthritis (RA) patients. However, a large amount of serum Hex activity has its source from platelets, and the blood platelet-count is often increased in RA, which may have masked the ...

journal_title：Clinical biochemistry

authors： Casal JA,Mera A,Pérez LF,Tutor JC

更新日期：2002-09-01 00:00:00