当前位置： SCI文献检索 > CLINICAL BIOCHEMISTRY期刊下所有文献 > A rapid and specific method for the determination of oxytocinase (cystine aminopeptidase) activity using continuous flow analysis and S-benzyl-L-cysteine-p-nitroanilide as substrate.

A rapid and specific method for the determination of oxytocinase (cystine aminopeptidase) activity using continuous flow analysis and S-benzyl-L-cysteine-p-nitroanilide as substrate.

Abstract：

:1. An automated method is described for the assay of pregnancy serum cystine aminopeptidase (Oxytocinase, E.C. 3.4.1.10) using continuous flow analysis. S-Genzyl-L-cysteine-p-nitroanilide, the most highly specific substrate so far available for oxytocinase is employed in this assay. Comparison with the manual method from which it has been developed, gave a correlation coefficient of 0.98. Reaction of oxytocinase on the substrate liberated p-nitroaniline, the absorption of which is measured at 410nm. 2. Blank values are eliminated by the incorporation of a dialyzer in the manifold design and thereby effectively increasing the rate of assay sample to 40 per hour. The lag phase (a) of the continuous flow analysis is 0.17 min while the exponential phase (b) is 0.31 min. Using a 2:1 sample to wash ration the carry-over is 3% at a 95% of steady states.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Small CW,Watkins WB

doi

10.1016/s0009-9120(75)91273-4

subject

Has Abstract

pub_date

1975-04-01 00:00:00

pages

124-32

issue

2

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(75)91273-4

journal_volume

8

pub_type

杂志文章

相关文献

CLINICAL BIOCHEMISTRY文献大全
  • Systemic redox biomarkers and their relationship to prognostic risk markers in autosomal dominant polycystic kidney disease and IgA nephropathy.

    abstract：BACKGROUND:Oxidative stress is evident from an early stage in chronic kidney disease (CKD). Therefore, we investigated redox biomarkers in polycystic kidney disease (ADPKD) and IgA nephropathy (IGAN). METHODS:This is a case-control study with three groups: ADPKD (n = 54), IGAN (n = 58) and healthy controls (n = 86). T...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2018.04.010

    authors： Tariq A,Mansoor MA,Marti HP,Jonsson G,Slettan A,Weeraman P,Apeland T

    更新日期：2018-06-01 00:00:00

  • Random uncertainty of photometric determination of hemolysis index on the Abbott Architect c16000 platform.

    abstract：BACKGROUND:Automatic photometric determination of the hemolysis index (HI) on serum and plasma samples is central to detect potential interferences of in vitro hemolysis on laboratory tests. When HI is above an established cut-off for interference, results may suffer from a significant bias and undermine clinical relia...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2018.01.009

    authors： Aloisio E,Carnevale A,Pasqualetti S,Birindelli S,Dolci A,Panteghini M

    更新日期：2018-07-01 00:00:00

  • Cytokine promoter polymorphisms in Taiwanese patients with Graves' disease.

    abstract：OBJECTIVES:This study aimed to examine the association between promoter polymorphisms of Th1 and Th2 cytokine genes [interleukin-4 (IL-4 T-34C, A-81G, C-285T and T-589C), IL-6 (G-174C), IL-10 (A-592C and T-819C) and tumour necrosis factor-alpha (TNF-alpha G-238A and G-308A)] and Graves' disease (GD) in Taiwanese popula...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2006.11.009

    authors： Shiau MY,Huang CN,Yang TP,Hwang YC,Tsai KJ,Chi CJ,Chang YH

    更新日期：2007-02-01 00:00:00

  • Relation of genetic polymorphisms of apolipoprotein E, angiotensin converting enzyme, apolipoprotein B-100, and glycoprotein IIIa and early-onset coronary heart disease.

    abstract：OBJECTIVE:Apolipoprotein E (APOE) E4, apolipoprotein B-100 (APOB) Q3611 allele, the angiotensin converting enzyme (ACE) deletion (D) allele and glycoprotein IIIa (GP3A) P33 mutant allele are reported to predispose to early-onset coronary heart disease (CHD). These associations were not all confirmed in more recent stud...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(99)00017-x

    authors： Nassar BA,Dunn J,Title LM,O'Neill BJ,Kirkland SA,Zayed E,Bata IR,Cantrill RC,Johnstone J,Dempsey GI,Tan MH,Breckenridge WC,Johnstone DE

    更新日期：1999-06-01 00:00:00

  • Problems with the estimation of urine protein by automated assays.

    abstract：OBJECTIVES:Most clinical laboratories replaced their manual precipitation techniques for the determination of urinary protein with automated dye binding assays or benzethonium chloride-turbidimetric assays. Few studies have validated these assays for the measurement of urinary proteins in the normal range. DESIGN AND ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2004.12.010

    authors： Dube J,Girouard J,Leclerc P,Douville P

    更新日期：2005-05-01 00:00:00

  • Molecular and hematological characteristics of a novel form of α-globin gene triplication: the hemoglobin St.Luke's-Thailand [α95(G2)Pro→Arg] or Hb St. Luke's [A2] HBA2.

    abstract：OBJECTIVES:This study aims to report a hitherto un-described α-globin gene triplicated allele with a novel hemoglobin (Hb) variant on α2-globin gene, Hb St.Luke's-Thailand [α95(G2)Pro-Arg]. DESIGNS AND METHODS:A study was done on a 1.5-year-old Thai boy who showed pallor and jaundice after birth. Hb analysis was perfo...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2013.01.022

    authors： Singha K,Fucharoen G,Jetsrisuparb A,Fucharoen S

    更新日期：2013-05-01 00:00:00

  • Histamine quantification in human plasma using high resolution accurate mass LC-MS technology.

    abstract：BACKGROUND:Histamine (HA) is a small amine playing an important role in anaphylactic reactions. In order to identify and quantify HA in plasma matrix, different methods have been developed but present several disadvantages. Here, we developed an alternative method using liquid chromatography coupled with an ultra-high ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.08.012

    authors： Laurichesse M,Gicquel T,Moreau C,Tribut O,Tarte K,Morel I,Bendavid C,Amé-Thomas P

    更新日期：2016-01-01 00:00:00

  • High-throughput genotyping with infrared fluorescence allele specific hybridization (iFLASH): a simple, reliable and low-cost alternative.

    abstract：OBJECTIVES:To develop and validate a novel genotyping approach, named infrared Fluorescence Allele Specific Hybridization (iFLASH), which combines the principles of allele specific oligonucleotide (ASO) hybridization with the advanced possibilities of infrared imaging. DESIGN AND METHODS:As an example, we genotyped th...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2006.02.012

    authors： van Himbergen TM,Voorbij HA,Barendrecht AD,van Rijn BB,Brambilla R,van Tits LJ,Roest M

    更新日期：2006-07-01 00:00:00

  • Utility of CA 15-3 and CEA in monitoring breast cancer patients with bone metastases: special emphasis on "spiking" phenomena.

    abstract：OBJECTIVES:CA 15-3 and CEA are considered useful tumor markers in monitoring breast cancer patients. This study was undertaken to specifically evaluate the transient elevations in these markers that are observed during systemic treatment for metastatic disease. This phenomenon has been termed "spiking." DESIGN AND MAT...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(96)00133-6

    authors： Yasasever V,Dinçer M,Camlica H,Karaloğlu D,Dalay N

    更新日期：1997-02-01 00:00:00

  • Evaluation of the analytical performance of the Boehringer Mannheim Elecsys 2010 immunoanalyzer.

    abstract：OBJECTIVES:We evaluated the analytical performance of the Elecsys 2010 immunoanalyzer (Boehringer Mannheim Canada), which is based on a new detection technology, electrochemical luminescence. DESIGN AND METHODS:We used six representative assays from the initial launch menu of the instrument: thyroid stimulating hormon...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(98)00002-2

    authors： Forest JC,Massé J,Lane A

    更新日期：1998-03-01 00:00:00

  • Apolipoprotein B-100 kinetics and static plasma indices of triglyceride-rich lipoprotein metabolism in overweight men.

    abstract：OBJECTIVE:We examined the association of plasma apolipoprotein (apoB) B-48, remnant-like particle (RLP)-cholesterol and non-HDL cholesterol concentrations with apoB-100 kinetics in overweight-obese men. METHODS AND RESULTS:Very-low density lipoprotein (VLDL), intermediate-density lipoprotein (IDL) and low-density lipo...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2005.05.009

    authors： Chan DC,Watts GF,Ng TW,Uchida Y,Sakai N,Yamashita S,Barrett PH

    更新日期：2005-09-01 00:00:00

  • Serum retinol binding protein 4 in patients with familial partial lipodystrophy.

    abstract：OBJECTIVE:To determine Retinol Binding Protein 4 (RBP4) levels in patients with Familial Partial Lipodystrophy (FPLD). METHODS:Ten patients with FPLD and a control group (9 patients) were selected to participate in the study. RESULTS:RBP4-log levels were lower in patients with FPLD in comparison to control group (1.5...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2009.03.006

    authors： Godoy-Matos AF,Moreira RO,MacDowell R,Bendet I,Mory PB,Moises RS

    更新日期：2009-07-01 00:00:00

  • Effect of whole soy and purified isoflavone daidzein on renal function--a 6-month randomized controlled trial in equol-producing postmenopausal women with prehypertension.

    abstract：OBJECTIVES:The aim of the study was to examine the long-term effect of commonly used whole soy foods (soy flour) and purified daidzein (one major isoflavone and the precursor of equol) on renal function among prehypertensive postmenopausal women who are also equol producers, a population most likely to benefit from soy...

    journal_title：Clinical biochemistry

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/j.clinbiochem.2014.05.054

    authors： Liu ZM,Ho SC,Chen YM,Tang N,Woo J

    更新日期：2014-09-01 00:00:00

  • Resolution of age-dependent reference intervals: polynomial regression methodology with applicability to plasma zinc levels in a childhood population.

    abstract：:We present a systematic parametric method for resolving age-related reference intervals based on differential-polynomial regression analysis. Application of this method to plasma zinc concentrations in a childhood population results in a third degree polynomial expression from which two statistically independent age-r...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(79)90056-0

    authors： Butrimovitz GP,Purdy WC

    更新日期：1979-02-01 00:00:00

  • Comparative study of respiratory burst induced by phorbol ester and zymosan in human granulocytes.

    abstract：OBJECTIVES:We have comparatively evaluated respiratory burst and some steps of its signaling in human granulocytes towards different stimuli. MATERIALS AND METHODS:An alternative method to remove erythrocytes by dextran differential sedimentation was employed. Respiratory burst (RB) was assessed in a flow cytometer by...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2005.10.005

    authors： Lunardi F,Lima JH,Assreuy J

    更新日期：2006-01-01 00:00:00

  • Correlation of increased oxidative stress to body weight in disease-free post menopausal women.

    abstract：OBJECTIVES:Oxidative stress increases postmenopausally, an effect attributed to aging. Increase in body weight generally accompanies menopause. Obesity per se also seems to increase oxidative stress. The question is whether postmenopausal increase in oxidative stress is related to body weight, or is only a function of ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2009.03.019

    authors： Mittal PC,Kant R

    更新日期：2009-07-01 00:00:00

  • Biochemical and biological aspects of the plasminogen activation system.

    abstract：:Plasminogen activators (PAs) are specific proteolytic enzymes which convert the inactive proenzyme plasminogen to plasmin. The plasmin formed is a potent and nonspecific protease which cleaves blood fibrin clots and several other extracellular proteins. In addition to their primary role in the initiation of fibrinolys...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/0009-9120(90)90601-p

    authors： Mayer M

    更新日期：1990-06-01 00:00:00

  • A comparative study of three approaches to the routine quantitation of human serum proteins.

    abstract：:The Laser Nephelometer PDQTM (Hyland Division, Travenol Laboratories Inc.) and the Abbott Bichromatic Analyser 100 (Abbott Laboratories) were compared with a radial immunodiffusion method. Seventy-eight serum specimens collected during routine blood testing were aliquoted and quantitated by the three procedures descri...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(78)90126-1

    authors： Bruver RM,Salkie ML

    更新日期：1978-06-01 00:00:00

  • Fluorometric and time-resolved immunofluorometric assays for protein-tyrosine phosphatase activity.

    abstract：OBJECTIVE:To develop sensitive nonisotopic assays for protein-tyrosine phosphatase (PTP) activity. METHODS:The fluorometric assay is based on the fact that phosphotyrosine but not tyrosine forms highly fluorescent complexes with Tb3+. Thus, PTP activity can be followed by measuring the decrease of fluorescence due to ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/0009-9120(95)02026-8

    authors： Galvan B,Christopoulos TK

    更新日期：1996-04-01 00:00:00

  • Comparison by LC-MS and MALDI-MS of prostate-specific antigen from five commercial sources with certified reference material 613.

    abstract：OBJECTIVE:Prostate-specific antigen (PSA) samples from five commercial sources were compared with PSA from the European Commission Community Bureau of Reference, known as CRM 613, using liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). DESIGN ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2004.10.016

    authors： Satterfield MB,Welch MJ

    更新日期：2005-02-01 00:00:00

  • Solid phase measurements of antibody and lectin binding to xenogenic carbohydrate antigens.

    abstract：OBJECTIVES:In future pig-to-man xenotransplantation it is important to master tools that identify potentially xenogenic alphagalactose (Galalpha) antigens in the doner tissue. DESIGN AND METHODS:We have measured the binding potentials of Galalpha detecting lectins and antibodies, including a naturally occurring subfra...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(03)00120-6

    authors： Kirkeby S,André S,Gabius HJ

    更新日期：2004-01-01 00:00:00

  • Changes in serum obestatin, preptin and ghrelins in patients with Gestational Diabetes Mellitus.

    abstract：OBJECTIVES:The present study aims to establish the levels of acylated ghrelin, desacylated ghrelin, obestatin and preptin, during pregnancy and the postpartum period in pregnant women with Gestational Diabetes Mellitus (GDM) and healthy pregnancy women. DESIGN AND METHODS:The study registered 20 pregnant women with GD...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2011.08.1131

    authors： Baykus Y,Gurates B,Aydin S,Celik H,Kavak B,Aksoy A,Sahin I,Deniz R,Gungor S,Guzel SP,Minareci Y

    更新日期：2012-02-01 00:00:00

  • Characterization of a novel isolated deletion of the exon 3 within the CFTR gene: Relevance for phenotypic expression and genetic counseling.

    abstract：OBJECTIVES:To characterize a novel deletion of exon 3 of CFTR gene and to evaluate the implications in Cystic Fibrosis (CF) care and genetic counseling. DESIGN AND METHODS:We performed a wide mutational analysis of CFTR gene, using reverse dot blot, Multiplex Ligation-dependent Probe Amplification (MLPA) assay and Rea...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2011.03.140

    authors： Tomaiuolo AC,Sirleto P,Centrone C,Surace C,Alghisi F,Petrocchi S,Lombardo A,Rossi M,Torricelli F,Lucidi V,Angioni A

    更新日期：2011-07-01 00:00:00

  • Beta-hexosaminidase isoenzyme profiles in serum, plasma, platelets and mononuclear, polymorphonuclear and unfractionated total leukocytes.

    abstract：OBJECTIVES:The relative proportion in percentage of the isoenzyme A of beta-hexosaminidase (Hex) is the single discriminatory function most frequently used for the biochemical screening of heterozygote Tay-Sachs disease carriers. It has been suggested that the assay of the Hex isoenzymes in homogeneous cell preparation...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2005.05.016

    authors： Casal JA,Cano E,Tutor JC

    更新日期：2005-10-01 00:00:00

  • Influence of apolipoprotein(a) phenotype on lipoprotein(a) quantification: evaluation of three methods.

    abstract：:Three commercially available assays (an enzyme-linked immunosorbent assay ELISA, an immunoradiometric assay, IRMA, and a nephelometric assay) for the determination of lipoprotein(a) [Lp(a)] were compared with respect to the dependency of these assays on the various apolipoprotein(a) [apo(a)] isoforms. Although there w...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/0009-9120(94)00052-w

    authors： Leus FR,Leerink CB,Prins J,van Rijn HJ

    更新日期：1994-12-01 00:00:00

  • The function, composition, and particle size of high-density lipoprotein were severely impaired in an oliguric phase of hemorrhagic fever with renal syndrome patients.

    abstract：BACKGROUND:Patients suffering from hemorrhagic fever with renal syndrome (HFRS) often showed strikingly reduced high-density lipoprotein (HDL)-cholesterol levels during the oliguric phase, indicating severe alterations in lipoprotein metabolism. OBJECTIVE:To compare changes in the functions and composition of HDL, lip...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2007.10.007

    authors： Cho KH,Park SH,Park JE,Kim YO,Choi I,Kim JJ,Kim JR

    更新日期：2008-01-01 00:00:00

  • Val-9Ala and Ile+58Thr polymorphism of MnSOD in Parkinson's disease.

    abstract：OBJECTIVES:To investigate the polymorphism distribution of Val-9Ala and Ile+58Thr of the Mn-superoxide dismutase (Mn-SOD) gene among subjects with Parkinson's disease (PD) by analyses of genders and clinical severity. DESIGN AND METHODS:We examined the DNA genotypes of Val-9Ala and Ile+58Thr from 295 PD subjects and 1...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2010.05.009

    authors： Wang V,Chen SY,Chuang TC,Shan DE,Soong BW,Kao MC

    更新日期：2010-08-01 00:00:00

  • The effect of nephropathy on plasma sphingosine 1-phosphate concentrations in patients with type 2 diabetes.

    abstract：OBJECTIVES:Sphingosine 1-phosphate (S1P) is carried in plasma by the HDL particles and albumin. It mediates several protective functions of HDL. Because of its barrier-enhancing effect, it has attracted attention in diseases associated with endothelial dysfunction. We examined the impact of circulating levels of S1P in...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.08.001

    authors： Bekpinar S,Yenidunya G,Gurdol F,Unlucerci Y,Aycan-Ustyol E,Dinccag N

    更新日期：2015-12-01 00:00:00

  • Plasma and erythrocyte lipids in two families with heterozygous hypobetalipoproteinemia.

    abstract：:Erythrocyte morphology, kinematic viscosity, and membrane lipid composition have not been well documented in heterozygous hypobetalipoproteinemia (HBL). In our study of nine subjects with HBL from two kindreds, three presented with schistocytes (3-6%) while two of them also showed acanthocytosis (25-35%) on their peri...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(88)80020-1

    authors： Gheeraert P,De Buyzere M,Delanghe J,De Scheerder I,Bury J,Rosseneu M

    更新日期：1988-12-01 00:00:00

  • Potential markers of the atherosclerotic process in high-risk children.

    abstract：:Children whose parents had early coronary heart disease were investigated. In order to assess high-risk parameters serum total cholesterol (TC), total triglyceride (TT), high-density lipoprotein cholesterol (HDLC), low-density lipoprotein cholesterol (LDLC), lipid peroxide (LP), prostacyclin (PGI2), thromboxane (TX) l...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/0009-9120(91)90541-l

    authors： Szamosi T,Mihai K,Peto J,Makary A,Kramer J

    更新日期：1991-04-01 00:00:00