Abstract:
OBJECTIVES:We evaluated the analytical performance of the Elecsys 2010 immunoanalyzer (Boehringer Mannheim Canada), which is based on a new detection technology, electrochemical luminescence. DESIGN AND METHODS:We used six representative assays from the initial launch menu of the instrument: thyroid stimulating hormone (TSH), free thyroxine (FT4), troponin T, human chorionic gonadotropin (hCG), carcinoembryonic antigen (CEA), and prostate specific antigen (PSA). Within-run and between-day imprecision were evaluated using pools of human specimens at low, mid and high concentrations. Linearity was evaluated by diluting specimens with high analyte concentrations with specimens that had a low level of this analyte. Carry over and hook effect were investigated using specimens with high concentrations of hCG. Functional sensitivity was studied by running low TSH specimens over 21 daily runs, and by comparing the scatterplot of FT4 as a function of TSH. Over 100 specimens distributed across the analytical range were analyzed with two comparison methods: ES 300 (Boehringer Mannheim Canada) and AxSYM (Abbott Laboratories). RESULTS:Within-run and between-day imprecisions were less than 4% and 10%, respectively, for most assays. All assays were linear over the whole analytical range. Carry over was minimal (< 0.0002%). A hook effect was present for hCG levels greater than 560,000 U/L. The functional sensitivity of the TSH assay was lower than 0.02 mlU/L. Correlation coefficients were all > 0.94. Small proportional errors were observed in comparison studies for the CEA and PSA assays. CONCLUSIONS:The Elecsys 2010 system was shown to have an acceptable analytical performance for the rapid analysis of a wide variety of analytes. The hook effect observed with hCG assay would imply that the laboratory informs the clinicians of the possibility of falsely low values in trophoblastic diseases or that all specimens with values greater than 3000 U/L are reassayed after dilution.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Forest JC,Massé J,Lane Adoi
10.1016/s0009-9120(98)00002-2subject
Has Abstractpub_date
1998-03-01 00:00:00pages
81-8issue
2eissn
0009-9120issn
1873-2933pii
S0009912098000022journal_volume
31pub_type
杂志文章abstract:BACKGROUND:L-Dopa decarboxylase (DDC) is a pyridoxal 5'-phosphate-dependent enzyme that was found to be involved in many malignancies. The aim of this study was to investigate the mRNA expression levels of DDC in prostate tissues and to evaluate its clinical utility in prostate cancer (CaP). METHODS:Total RNA was isol...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.04.026
更新日期:2008-10-01 00:00:00
abstract:OBJECTIVE:To investigate unusual free thyroxine (FT4) responses to T4 replacement doses in a hypothyroid patient with familial dysalbuminemic hyperthyroxinemia (FDH). METHODS:In this FDH hypothyroid patient, serum FT4 concentration by equilibrium dialysis and T4, triiodothyronine (T3), and thyroid stimulating hormone ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(95)02016-0
更新日期:1996-02-01 00:00:00
abstract:BACKGROUND:Increased levels of circulating endostatin predicts cardiovascular morbidity and impaired kidney function in the general population. The utility of endostatin as a risk marker for mortality in the emergency department (ED) has not been reported. AIM:Our main aim was to study the association between plasma e...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2019.10.004
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:To determine the relationship between BiliChek TcB (Respironics, Marietta GA) and Doumas reference serum or plasma total bilirubin (TSB). DESIGN AND METHODS:Pooled samples with values assigned by the Doumas reference method were used to establish the relationship between a local laboratory and reference Dou...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.04.065
更新日期:2010-08-01 00:00:00
abstract::Retrospective analysis of DNA from paraffin-embedded fixed bone marrow biopsy specimens is possible if preceded by amplification of the DNA sequences of interest by the polymerase chain reaction (PCR). These fixed specimens yield degraded DNA that may not be suitable for direct analysis by conventional digestion and h...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(92)80051-h
更新日期:1992-04-01 00:00:00
abstract:BACKGROUND:Gliomas are tumors of the central nervous system derived from glial cells. They show cellular heterogeneity and lack specific diagnostic markers. Although a possible role for the eicosanoid cascade has been suggested in glioma tumorigenesis, the relationship between enzymes and receptors implicated in arachi...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.03.017
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:Given that both insufficient and excess maternal iodine have adverse consequences such as poor cognitive performance, delayed physical development and increased fetal and infant mortality, the determination of maternal iodine status is very important. In this study, we established and verified a method invol...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2020.03.009
更新日期:2020-08-01 00:00:00
abstract::Senescence is a process which, until quite recently, has been the subject of little scientific investigation. Even the word "senescence" is difficult to define, and complex methodological pitfalls have impeded progress. In the past few years, there have been exciting advances in understanding the physiological, cell b...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/0009-9120(92)80047-k
更新日期:1992-04-01 00:00:00
abstract:OBJECTIVES:To investigate the predictive value of cystatin C among patients diagnosed with non-ST-elevation acute coronary syndrome (nSTE-ACS). DESIGN AND METHODS:Admission serum samples from 245 nSTE-ACS patients were measured with a novel cystatin C immunoassay based on a dry-reagent, double monoclonal design. Creat...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2012.02.012
更新日期:2012-05-01 00:00:00
abstract:OBJECTIVE:We used single-strand conformational polymorphism (SSCP) to screen for mutations at nucleotides 833 and 919 of the cystathionine beta-synthase (CBS) gene in 13 patients with homocystinuria and 11 of their relatives. METHODS:Exon 8 of genomic DNA was selectively amplified by PCR using primers derived from int...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(96)00045-8
更新日期:1996-10-01 00:00:00
abstract:OBJECTIVE:Recently cortisol level in hair is supposed to be a good biomarker of chronic stress. We had developed a new method based on HPLC-FLU for identification of cortisol in human hair and measured cortisol distribution of each 1-cm segment along hair shaft. DESIGN AND METHODS:Hair sample was pulverized and incuba...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.01.014
更新日期:2010-05-01 00:00:00
abstract:OBJECTIVES:Recurrent miscarriage (RM) is a challenging medical problem because of its unknown pathogenesis and etiology in most of the cases. Recent studies suggest the role of persistent environmental pollutants such as organochlorine pesticides (OCPs) in the etiology of RM. The present study was conducted to investig...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2009.09.019
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVES:The plasma apolipoprotein B (apo B) concentrations have been considered to be a more accurate representation of atherogenic particles and it has been proposed that the formula LDL-C (mmol/L) = 0.41TC - 0.32TG + 1.70apo B - 0.27 is reliable for the estimation of LDL-C (Clin Chem 1997; 43: 808-15). We undertoo...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(00)00162-4
更新日期:2000-10-01 00:00:00
abstract:OBJECTIVES:To assess the medical applicability of CpG methylation as molecular markers for cancer diagnosis, we established a new system to determine DNA methylation based on TaqMan PCR combined with a methyl-binding-domain polypeptide 2. DESIGN AND METHODS:We evaluated the diagnostic applicability of this approach by...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2009.03.017
更新日期:2009-07-01 00:00:00
abstract:OBJECTIVES:Stable isotope dilution coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the sensitive method for screening for various inherited metabolic disorders using dried blood spots (DBSs). We present a method for LC-MS/MS determination of succinyladenosine (SAdo) and succinylaminoimidazole ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2014.10.004
更新日期:2015-01-01 00:00:00
abstract::Plasma levels of dehydroepiandrosterone sulfate, dehydroepiandrosterone and delta 5-androstenediol were measured in several women throughout pregnancy. The levels of both dehydroepiandrosterone and its sulfate decreased while those of delta 5-androstenediol did not. The levels of binding of the latter steroid to plasm...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(80)90795-x
更新日期:1980-06-01 00:00:00
abstract:OBJECTIVES:The circulating lipoproteins may cause some abnormalities in platelet composition and function in hypercholesterolemia. The aim of this study was to investigate whether platelet apoptosis, platelet activation, platelet aggregation, platelet-leukocyte aggregate (PLA) formation and lipid peroxidation occur sim...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2005.09.005
更新日期:2005-12-01 00:00:00
abstract:OBJECTIVES:Pseudoxanthoma elasticum (PXE) is a hereditary disorder predominantly affecting the skin, retina and vascular system. The aim of this study was to measure cell adhesion molecules in PXE patients. DESIGN AND METHODS:Soluble P-, E- and L-selectins were measured in 61 non-consanguineous PXE patients. The distr...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2007.12.009
更新日期:2008-04-01 00:00:00
abstract::Children whose parents had early coronary heart disease were investigated. In order to assess high-risk parameters serum total cholesterol (TC), total triglyceride (TT), high-density lipoprotein cholesterol (HDLC), low-density lipoprotein cholesterol (LDLC), lipid peroxide (LP), prostacyclin (PGI2), thromboxane (TX) l...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(91)90541-l
更新日期:1991-04-01 00:00:00
abstract:OBJECTIVES:An overview of biochemical markers of bone metabolism is presented along with indications for their clinical utilization. DESIGN AND METHODS:The structure, cyclical metabolism, and hormone regulation of bone is reflected by markers of resorption, formation and/or turnover. Markers of resorption representing...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/s0009-9120(97)00113-6
更新日期:1997-12-01 00:00:00
abstract::Immunoturbidimetric assays for specific proteins are available on "open system" clinical chemistry analyzers. The analytical performance of nine immunoturbidimetric specific protein assays (C3, C4, CRP, Haptoglobin, IgA, IgG, IgM, RF, and Transferrin) was compared to immunonephelometry. Testing was performed on the Ab...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2009.06.016
更新日期:2009-10-01 00:00:00
abstract::Suspected myocardial infarction (MI) is a common reason for emergency hospital attendance and admission. Cardiac biomarker measurement is an essential element of diagnostic assessment of suspected MI. Although the cost of a routinely available biomarker may be small, the large patient population and consequences in te...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2014.08.012
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVE:To evaluate relationships between apolipoprotein B (Apo B), LDL cholesterol (LDL-C), and non-HDL-C in high-risk patients treated with lipid-lowering therapy. DESIGN AND METHODS:This post-hoc analysis calculated LDL-C and non-HDL-C levels corresponding to an Apo B of 0.9 g/L following treatment with 1) statin...
journal_title:Clinical biochemistry
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.clinbiochem.2011.02.008
更新日期:2011-06-01 00:00:00
abstract:OBJECTIVE:We examined the association of plasma apolipoprotein (apoB) B-48, remnant-like particle (RLP)-cholesterol and non-HDL cholesterol concentrations with apoB-100 kinetics in overweight-obese men. METHODS AND RESULTS:Very-low density lipoprotein (VLDL), intermediate-density lipoprotein (IDL) and low-density lipo...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2005.05.009
更新日期:2005-09-01 00:00:00
abstract:OBJECTIVES:Delayed graft function (DGF) is still a major issue in kidney transplantation. Plasma and urine neutrophil gelatinase-associated lipocalin (NGAL) were evaluated in a population of kidney donors and recipients to investigate their performance to predict early renal function. DESIGN AND METHODS:Plasma (pNGAL)...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2014.06.079
更新日期:2014-10-01 00:00:00
abstract::The effect of altered thyroid function on serum fructosamine concentrations was investigated in 31 untreated hyperthyroid patients, 18 short-term hypothyroid patients (i.e., 20 days after withdrawal of thyroid hormone suppressive therapy for thyroid cancer), 7 untreated long-term hypothyroid patients, and 25 age-match...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(88)90007-0
更新日期:1988-06-01 00:00:00
abstract:OBJECTIVES:In the process of obtaining a driver's license, a healthy 28year old man presented increased levels of disialo-transferrin (TF) (approx. 20%, ref. value<2) by HPLC analysis of TF isoforms (%CDT), while other markers of excessive alcohol consumption (PEth, MCV and γ-GT) were in the normal range. The objective...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2015.12.001
更新日期:2016-04-01 00:00:00
abstract:INTRODUCTION:Bisalbuminemia is a genetic condition in which an albumin variant is found in serum in addition to normal albumin. METHODS AND MATERIALS:Serum protein electrophoresis using the Sebia HYDRASYS electrophoresis system was performed on an 84 year old male. RESULTS:Serum protein electrophoresis showed a singl...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2011.10.005
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVES:Lipoprotein(a) [Lp(a)] can be oxidized by copper in vitro in a way comparable to low-density lipoprotein (LDL). We sought to determine whether the susceptibility of Lp(a) to oxidation is correlated with the susceptibility of autologous heterogeneous LDL, with apolipoprotein(a) [apo(a)] molecular size, or wit...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(02)00438-1
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND:The aim of this study was to investigate whether BGD activity is of additional value in the assessment of pulmonary inflammation caused by coal dust exposure. DESIGN AND METHODS:Ex-coalminers were included in this study. Forty-eight healthy male subjects, without a relevant medical history, were used as con...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(99)00070-3
更新日期:1999-11-01 00:00:00