Abstract：
:The use of point-of-care (POC) immunoassays has increased significantly and the menu of analytes continues to expand. Most of the rapid immunoassays are currently based on simple manual assay devices such as the immunochromatographic, agglutination, and immunofiltration assays. Although automated readers have recently been introduced at an increasing pace, the major benefit of these genuinely hand-portable assay devices is that they do not usually necessitate instrumentation but can be performed anywhere. Significant advances in assay and detection technologies have, however, recently facilitated the introduction of truly quantitative, sophisticated immunoassay methods to POC settings as well, with the analytical performance characteristics approaching those of conventional laboratory assays. Furthermore, innovative assay technologies such as those based on immunosensors have been introduced to POC testing (POCT) without ever being employed in clinical laboratories. However, further simplification of the assay procedures and analyzers is still feasible, and strong efforts are directed towards the development of miniaturized and simple, yet sensitive and quantitative, novel assay technologies to keep up with the increasing expectations set on future POC immunotesting.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
von Lode Pdoi
10.1016/j.clinbiochem.2005.03.008subject
Has Abstractpub_date
2005-07-01 00:00:00pages
591-606issue
7eissn
0009-9120issn
1873-2933pii
S0009-9120(05)00089-5journal_volume
38pub_type
杂志文章,评审abstract：:Urinary amylase was estimated by chromogenic (amylochrome Roche) as well as enzymatic methods (SKI and Beckman: substrate starch and substrate maltotetraose respectively). Random and timed urines (24 hour collections) were analysed. Clearances of amylase gave different results dependent upon the amylase-test used and ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(79)80125-3
更新日期：1979-12-01 00:00:00
abstract：OBJECTIVE:We performed a side-by-side comparison between three stain-then-lyse commercially available methods (Ortho-mune Lysing solution, FACS lysing solution and ImmunoPrep reagent system) and a lyse-then-stain method using hypotonic NH4Cl. The major difference between these methods is that only in the latter the ali...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(98)00011-3
更新日期：1998-04-01 00:00:00
abstract：:Cytosolic proteins that specifically bind cyclosporine A and FK-506 were isolated and purified from the JURKAT human T-helper cell line. These binding proteins were purified by affinity, molecular weight exclusion and weak cation exchange column chromatography. Radiolabeled cyclosporine A specifically bound to a appro...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(91)90252-a
更新日期：1991-02-01 00:00:00
abstract：OBJECTIVES:To insure commutability (equivalence) between whole blood and plasma glucose values and to assess potential interferences in glucose oxidase methods. DESIGN AND METHODS:We compared plasma glucose results by the Ektachem 700 analyzer to glucose results on whole blood by the Nova Stat Profile 6, and by the YS...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(95)02010-1
更新日期：1996-02-01 00:00:00
abstract：OBJECTIVES:Researchers have proposed that cancer stem cells (CSCs) are the driving force of tumorigenesis, recurrence, and metastases, contributing to the failure of some cancer treatments. Water bath hyperthermia, a novel treatment for cancer, can be highly effective when combined with chemotherapy, radiotherapy, or i...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2013.08.023
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVE:Heat-mediated ELISA (HELISA) technique has been reported to shorten ELISA timings and has major clinical implications in diagnostic field [Bora U, Kannan K, Nahar P. Heat mediated enzyme linked immunosorbent assay. J Immunol Methods 2004; 293: 43-50]. Objective of this study is to find out whether anti-MBL au...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2007.09.016
更新日期：2008-01-01 00:00:00
abstract：OBJECTIVES:To ascertain whether or not the change in blood collection tubes for plasma glucose from fluoride/oxalate to citrate/fluoride/EDTA has had an effect upon the glucose results. METHODS:Plasma glucose results from fasting patients from 2007 to 2012 were extracted from the laboratory information system. The dat...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2014.01.011
更新日期：2014-05-01 00:00:00
abstract：:We assessed the diagnostic value of four commercially available methods for determining pancreatic lipase (LPS) in serum (the turbidimetric procedure from Boehringer, two enzymatic approaches from Kodak and Poli, and an immunochemical assay) in a population of 46 hospitalized patients with acute abdominal pain. In 31 ...
journal_title：Clinical biochemistry
pub_type： 临床试验,杂志文章
doi：10.1016/s0009-9120(05)80009-8
更新日期：1991-12-01 00:00:00
abstract：OBJECTIVES:Studies from both experimental animals and humans suggest that administration of exogenous DHEA may have beneficial endocrine-metabolic, immunologic and neurologic effects. Several groups have administered DHEA to humans, but to the best of our knowledge, no one at this point has published a summary of the r...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(99)00021-1
更新日期：1999-07-01 00:00:00
abstract：:Steroids play an important role in sperm production and quality. These hormones have been extensively studied in blood, but poorly investigated in semen. The purpose of our study was to evaluate the relationship between sperm quality and steroid profiles in blood and semen in a small cohort of young Swiss men. Another...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2018.03.008
更新日期：2018-12-01 00:00:00
abstract：:1. The following proteolytic enzymes were measured in muscles of control subjects and patients with muscular dystrophies and related neuromuscular diseases: an elastase-like enzyme, carboxypeptidase A, carboxypeptidase B and pyroglutamyl peptidase. 2. Elastase-like enzyme and carboxypeptidase B did not show significan...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(79)80001-6
更新日期：1979-04-01 00:00:00
abstract：OBJECTIVES:Hepcidin is the main regulator of systemic iron homeostasis and its expression is modulated by iron status, hypoxia, erythroid factors and inflammation. The aim of our study was to examine a relationship between level of hepcidin and iron status, erythropoietic activity, hypoxia and inflammation in exacerbat...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2015.07.010
更新日期：2015-12-01 00:00:00
abstract：:Increased hemoglobin A1 (HbA1) occurs in diabetes mellitus as a direct consequence of hyperglycemia, which causes postsynthetic modification of hemoglobin proportional to blood glucose concentration. Glycosylation of hemoglobin is demonstrable also in vitro, not only by glucose, but with other reducing sugars such as ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(82)90556-2
更新日期：1982-06-01 00:00:00
abstract：OBJECTIVE:This study was conducted to investigate the potential role of intravascular oxidative stress in inexplicable episodes of coronary artery spasm (CAS) during coronary angiography (CAG). DESIGN AND METHODS:Serial patients with stable angina scheduled for routine CAG were prospectively evaluated. Patients with c...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.01.021
更新日期：2009-05-01 00:00:00
abstract：:The ES 300 system, a fully automated multichannel immunoassay analyzer, was evaluated simultaneously for 9 weeks in four major centers. Precision, accuracy, carryover, comparison to in-house methods, and interferences were assessed for the following 17 tests: T4, T3, FT4, TSH, TBK, TBG, LH, FSH, prolactin, HCG, digoxi...
journal_title：Clinical biochemistry
pub_type： 杂志文章,多中心研究
doi：10.1016/0009-9120(93)90017-z
更新日期：1993-02-01 00:00:00
abstract：OBJECTIVE:To develop sensitive nonisotopic assays for protein-tyrosine phosphatase (PTP) activity. METHODS:The fluorometric assay is based on the fact that phosphotyrosine but not tyrosine forms highly fluorescent complexes with Tb3+. Thus, PTP activity can be followed by measuring the decrease of fluorescence due to ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(95)02026-8
更新日期：1996-04-01 00:00:00
abstract：:To ensure effective antimicrobial or tumouricidal function, phagocytic cells must maintain their cytoplasmic pH (pHi) at a level conductive to optimal intracellular enzyme activity. The mechanisms by which neutrophils and macrophages regulate their cytoplasmic pH include bicarbonate-independent ion transport systems, ...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/0009-9120(91)80014-t
更新日期：1991-06-01 00:00:00
abstract：:Ischemic heart disease is the primary cause of death worldwide. The pathophysiological process of cardiovascular diseases is linked to atheromatous plaque formation, while plaque rupture releases thrombogenic elements, which lead to activation of platelets, blood clotting and formation of thrombi. Platelet inhibitors ...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2014.03.005
更新日期：2014-05-01 00:00:00
abstract：AIM:Medical laboratories are the key partners in patient safety. Laboratory results influence 70% of medical diagnoses. Quality of laboratory service is the major factor which directly affects the quality of health care. The clinical laboratory as a whole has to provide the best patient care promoting excellence. METH...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2008.09.011
更新日期：2009-03-01 00:00:00
abstract：:The investigation of oxidative stress (OS), its mechanisms and connections with human diseases, is a topic of interest with more than 36,000 PubMed citations to date. The OS can be approached either from the perspective of pro-oxidation, either of anti-oxidation, and both can be investigated considering individual che...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2016.11.037
更新日期：2017-04-01 00:00:00
abstract：:Generally acid phosphatase (ACP) assay is used for testing cases of alleged rape. Comparison of three different chemical methods for Acid Phosphatase (Andersch's method with p-nitrophenyl-phosphate substrate and tartrate inhibitor (A-Tart), Roy's method with thymolphthalein phosphate substrate (R-TMP), and Babson's me...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(77)92784-9
更新日期：1977-10-01 00:00:00
abstract：OBJECTIVES:In 2004, a novel grading system for papillary non-invasive bladder cancer was introduced; low grade (LG) and high grade (HG) in lieu of the former G1, G2, G3. This change allowed for increased reproducibility as well as diminished interobserver variability in histopathological grading among individual pathol...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2011.09.013
更新日期：2012-01-01 00:00:00
abstract：:The purpose of this report is to evaluate whether a new, simple, non-invasive method for chymotrypsin measurement in stools is useful for the diagnosis of exocrine pancreatic insufficiency in cystic fibrosis (CF). A hundred children aged from 2 months to 12 years were tested: 50 children had been admitted for chronic ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(85)80046-1
更新日期：1985-08-01 00:00:00
abstract：OBJECTIVE:To assess the generation of reactive oxygen species (ROS) and the involvement of the main antioxidant pathways in low/intermediate-1-risk myelodysplastic syndromes (MDS) with iron overload (IOL). METHODS:We examined the levels of superoxide anion (O2-), hydrogen peroxide (H2O2), antioxidants (glutathione, GS...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2017.05.018
更新日期：2017-11-01 00:00:00
abstract：:In order to evaluate cardiovascular risk, we analyzed the lipid composition of HDL and the presence of lipoprotein(a) [Lp(a)] by both agarose gel electrophoresis and enzyme-linked immunoassay (ELISA). In 681 plasmas we found a close correspondence between the existence of a visible sinking pre-beta lipoprotein band an...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(93)90117-o
更新日期：1993-10-01 00:00:00
abstract：OBJECTIVE:To characterize the lipid-related atherogenic risk factors in iron deficiency anaemia (IDA) patients. DESIGN AND METHODS:Twenty IDA women were compared to healthy age-matched controls. Lipoprotein profile, cholesteryl ester transfer protein (CETP), paraoxonase (PON) 1 and lipoprotein-associated phospholipase...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.10.003
更新日期：2010-03-01 00:00:00
abstract：:To elucidate the diagnostic relevance of biliary conjugated bilirubin, biliary bilirubin from normal volunteers (NV), patients with Gilbert's syndrome (GS) and Crigler-Najjar syndrome type II (C-N II), and from various rat strains was fractionated. Biliary bilirubin diglucuronide (BDG) was present at lower levels, and...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(90)80024-d
更新日期：1990-04-01 00:00:00
abstract：OBJECTIVES:To develop a method for the determination of succinylacetone (SA) in dried blood spots (DBS) using tandem mass spectrometry (MS/MS). METHODS:SA was extracted from DBS with an acetonitrile and water solution (80:20 by volume) containing formic acid and hydrazine hydrate (both at 0.1% by volume), and analyzed...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2004.07.006
更新日期：2004-11-01 00:00:00
abstract：:Fluoride is a potent therapeutic agent that increases spinal bone density in osteoporotic subjects. Based on work with animal cells previously, we proposed fluoride acts by inhibiting phosphotyrosyl protein phosphatase (EC 3.1.3.48) activity in bone cells. The presence of fluoride sensitive acid phosphatase (EC 3.1.3....
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(92)80045-i
更新日期：1992-02-01 00:00:00
abstract：OBJECTIVES:To report the clinical and laboratory characteristics of urea cycle disorder (UCD) patients at a tertiary care center in a developing country. DESIGN AND METHODS:Retrospective study of clinical and laboratory data of UCD patients. RESULTS:Thirty-seven UCD patients were studied, 31 symptomatic (high risk) p...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.12.004
更新日期：2010-03-01 00:00:00
