Abstract:
OBJECTIVES:To insure commutability (equivalence) between whole blood and plasma glucose values and to assess potential interferences in glucose oxidase methods. DESIGN AND METHODS:We compared plasma glucose results by the Ektachem 700 analyzer to glucose results on whole blood by the Nova Stat Profile 6, and by the YSI 2300, both without hematocrit correction (YSIunc) and with two different hematocrit (Hct) corrections. The two correction methods were: (a) whole blood results multiplied by 1.11 (YSI1.11), and (b) whole blood results corrected for Hct with the programmed YSI algorithm (YSIcor). Several compounds were tested for interference. RESULTS:88% of YSIcor results agreed with plasma results within plus/minus 5%, and 78% of Nova and 74% of YSI1.11 results were within this limit. YSIcor results were unaffected by Hct and were valid even when erythrocyte size was abnormal. At low Hct, Nova results were falsely decreased, while YSI1.11 results were falsely elevated. The Ektachem was most affected by ascorbic acid, and the Nova was especially affected by acetaminophen. CONCLUSIONS:The YSI algorithm-corrected whole blood glucose results were commutable with plasma results and the YSI was unaffected by the compounds tested, including acetaminophen and acetylsalicylic acid.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Astles JR,Sedor FA,Toffaletti JGdoi
10.1016/0009-9120(95)02010-1subject
Has Abstractpub_date
1996-02-01 00:00:00pages
27-31issue
1eissn
0009-9120issn
1873-2933pii
0009-9120(95)02010-1journal_volume
29pub_type
杂志文章abstract:OBJECTIVES:Non-invasive prenatal aneuploidy testing (NIPT) by next-generation sequencing of circulating cell-free DNA in maternal plasma relies on chromosomal ratio (chrratio) measurements to detect aneuploid values that depart from euploid ratios. Diagnostic performances are known to depend on the fraction of fetal DN...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2018.06.015
更新日期:2018-09-01 00:00:00
abstract:OBJECTIVE:Immunosuppressive therapy after liver transplantation may be a risk for kidney dysfunction. This work was designed to determine whether Tamm-Horsfall Protein (THP) could be considered as a marker for nephrotoxicity. DESIGN AND METHODS:THP was determined by an ELISA method in serial 24-h urine from liver tran...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(02)00274-6
更新日期:2002-02-01 00:00:00
abstract::This study was performed to investigate the antioxidant mechanisms of polymorphonuclear leucocytes (PMN) in active stage of Behçet's Disease. PMN activities of myeloperoxidase (p < 0.02), superoxide dismutase (p < 0.001), catalase (p < 0.005), and glutathione peroxidase (p < 0.005) were significantly lower in the pati...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(94)90046-9
更新日期:1994-10-01 00:00:00
abstract::The effect of altered thyroid function on serum fructosamine concentrations was investigated in 31 untreated hyperthyroid patients, 18 short-term hypothyroid patients (i.e., 20 days after withdrawal of thyroid hormone suppressive therapy for thyroid cancer), 7 untreated long-term hypothyroid patients, and 25 age-match...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(88)90007-0
更新日期:1988-06-01 00:00:00
abstract:OBJECTIVES:The aim of the study was to examine the long-term effect of commonly used whole soy foods (soy flour) and purified daidzein (one major isoflavone and the precursor of equol) on renal function among prehypertensive postmenopausal women who are also equol producers, a population most likely to benefit from soy...
journal_title:Clinical biochemistry
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.clinbiochem.2014.05.054
更新日期:2014-09-01 00:00:00
abstract:OBJECTIVES:We investigated the relationships of biomarkers of various pathophysiologic pathways including high-sensitivity C-reactive protein (hs-CRP), lipocalin-2 (LCN2), myeloperoxidase (MPO) and matrix metalloproteinases 9 (MMP9) with mortality in stroke patients. DESIGN AND METHODS:hs-CRP, LCN2 and MPO concentrati...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2012.06.014
更新日期:2012-11-01 00:00:00
abstract:OBJECTIVES:The glutathione S-transferases (GSTs) play a critical role in protecting the colorectal mucosa. We investigated the efficacy of using the GSTs activity of plasma as a biomarker of risk for colorectal cancer. METHODS:GSTs activity was measured in the plasma of control individuals (n=39) and in the plasma, tu...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2005.04.004
更新日期:2005-07-01 00:00:00
abstract:OBJECTIVE:Determine the relation of xanthine oxidase (XO) activity and the outcome of septic patients and its relation to oxidative damage and clinical parameters of sepsis severity. DESIGN AND METHODS:Patients admitted over a 6-month period were enrolled. Patients were assigned to groups according to the diagnosis of...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.07.015
更新日期:2008-10-01 00:00:00
abstract:OBJECTIVE:Elevated concentrations of hCG beta core fragment (hCGβcf) are known to cause false-negative results in qualitative urine pregnancy test devices, but the pattern of urinary hCGβcf during normal pregnancy has not been well characterized. Here, we evaluate the relationship between urine hCG, hCGβcf, and hCG fre...
journal_title:Clinical biochemistry
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.clinbiochem.2016.04.006
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVES:The use of iodinated contrast media has grown in popularity in the past two decades, but relatively little attention has been paid to the possible interferential effects of contrast media on laboratory test results. Herein, we investigate medical contrast media interference with routine chemistry results obt...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2017.02.023
更新日期:2017-08-01 00:00:00
abstract::Urine contains an enormous amount of information. Well-standardized procedures for collection, transport, sample preparation and analysis should become the basis of an effective diagnostic strategy for urinalysis. As reproducibility of urinalysis has been greatly improved due to recent technological progress, preanaly...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2016.10.016
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to determine the reference values for serum cystatin C (CysC) with a particular focus on the effect of aging. DESIGN AND METHODS:The study was performed on a consecutive series of subjects (258 men and 396 women). Laboratory parameters and a detailed personal and family medical his...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2006.03.017
更新日期:2006-06-01 00:00:00
abstract:OBJECTIVES:Investigate the interference of Virox® disinfectant wipes containing hydrogen peroxide with Lifescan SureStep Flexx® glucose meter results. DESIGN AND METHODS:Glucose measurements of control and whole blood samples were performed before and after cleaning with Virox® wipes to reveal the kinetics of this int...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.09.012
更新日期:2010-12-01 00:00:00
abstract::Generally acid phosphatase (ACP) assay is used for testing cases of alleged rape. Comparison of three different chemical methods for Acid Phosphatase (Andersch's method with p-nitrophenyl-phosphate substrate and tartrate inhibitor (A-Tart), Roy's method with thymolphthalein phosphate substrate (R-TMP), and Babson's me...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(77)92784-9
更新日期:1977-10-01 00:00:00
abstract:OBJECTIVE:To externally validate a clinical decision rule incorporating heart fatty acid binding protein (h-FABP), high-sensitivity troponin (hs-cTn) and electrocardiogram (ECG) for the detection of acute myocardial infarction (AMI) on presentation to the Emergency Department. We also investigated whether this clinical...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2017.10.001
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVES:In widely used protocols for the collection and isolation of cord blood mononuclear cells, investigators are left with substantial volumes of diluted plasma which could be used for other measurements. The aim of this study was to ascertain the validity of umbilical cord blood (UCB) diluted plasma samples for...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2015.04.014
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVES:This study was undertaken to investigate the impact of the Pro12Ala (rs1801282) polymorphism of the peroxisome proliferator-activated receptor gamma-2 (PPARgamma-2) gene on obesity or body mass index (BMI) and plasma leptin, insulin, adiponectin and lipid levels in a sample of the Tunisian population. DESIG...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2009.08.019
更新日期:2009-11-01 00:00:00
abstract::We report a sensitive and specific method for the determination of AY-25,712 concentrations in rat, dog and human serum by liquid chromatography with UV detection. The detection limit is 25 ng/mL, based on 2 mL of serum or plasma. The method has been validated in human volunteers receiving single oral doses of AY-25,7...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(83)90112-1
更新日期:1983-08-01 00:00:00
abstract:OBJECTIVES:Association between vitamin D deficiency and excess of vitamin A as a potential risk factor of osteoporosis and fracture has been evaluated. DESIGN AND METHODS:232 healthy postmenopausal women were studied. Serum parameters were analyzed by standard methods and fat-soluble vitamins by an own HPLC method. QU...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.06.001
更新日期:2010-09-01 00:00:00
abstract::To date, pancreatic cancer (PDAC) can't be diagnosed early. Consequently, a majority of patient (80%) display an advanced disease that results in a low resection rate leading to an overall median survival of 4 to 6months. Accordingly, robust markers for the early diagnosis and prognosis of pancreatic cancer, or marker...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2013.03.019
更新日期:2013-07-01 00:00:00
abstract::A photon excitation, secondary target x-ray fluorescence system was used to measure concentrations of Cu, Zn, Br and Rb in whole blood and plasma of healthy adults. The samples preparation method and calculated limits of detection of the technique are reported. Correlation statistics for elemental concentrations have ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(77)91740-4
更新日期:1977-06-01 00:00:00
abstract:OBJECTIVES:Does abnormal betaine excretion persist? DESIGN AND METHODS:Patients (10) with abnormal excretion in 1998 were recalled in 2005. Subsequent urine samples were collected on 4 days from persistently abnormal subjects. RESULTS:Half the 1998 abnormal patients were abnormal in 2005. Only 1/20 controls was abnor...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2007.03.009
更新日期:2007-07-01 00:00:00
abstract::A simple method for the determination of triodothyronine (T3) uptake using I125-T3 and dextran coated charcoal is described. This reaction is time dependent after the addition of dextran coated charcoal. Effect of temperature, concentrations of I125-T3 and charcoal are reported. Within-run precision has been estimated...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(76)80009-4
更新日期:1976-02-01 00:00:00
abstract:INTRODUCTION:It has been suggested that ghrelin may play a role in growth hormone (GH) secretion to exercise. OBJECTIVE:The present study was designed to examine the effects of a single bout of circuit resistance exercise on plasma glucose, ghrelin, GH, and c-peptide, and cortisol. DESIGN AND METHODS:Fourteen volunte...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2006.05.009
更新日期:2006-10-01 00:00:00
abstract::We describe a patient who received intravenous dimethyl sulphoxide (DMSO) (as part of an experimental protocol) to control increased intracranial pressure. The patient died of a brain abscess. She developed extreme serum hyperosmolality from the DMSO with the osmolality peaking at 717 mosmol/kg. DMSO may induce extrem...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(83)90168-6
更新日期:1983-08-01 00:00:00
abstract:OBJECTIVE:To develop a tool to assess the clinical accuracy of glucose meter performance using an insulin dosing protocol to assess the frequency and extent of error in insulin dose categories. METHODS:Retrospective comparison of 1815 glucose meter and central laboratory glucose results obtained from 1698 critically i...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2019.06.001
更新日期:2019-08-01 00:00:00
abstract::Retrospective analysis of DNA from paraffin-embedded fixed bone marrow biopsy specimens is possible if preceded by amplification of the DNA sequences of interest by the polymerase chain reaction (PCR). These fixed specimens yield degraded DNA that may not be suitable for direct analysis by conventional digestion and h...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(92)80051-h
更新日期:1992-04-01 00:00:00
abstract:OBJECTIVES:To develop a tandem mass spectrometry (MS/MS) method for plasma free and total carnitine, incorporating acid hydrolysis for total carnitine measurement, for improved accuracy and sample throughput. DESIGN AND METHODS:Limit of detection (LOD), limit of quantitation (LOQ), linearity, accuracy, precision coeff...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.08.015
更新日期:2010-11-01 00:00:00
abstract:UNLABELLED:Frozen biospecimens are crucial for translational research and contain well-preserved nucleic acids and protein. However, the risks of freezer failure as well as space, cost, and environmental concerns of frozen biospecimens are substantial. OBJECTIVE:The purpose of the study was to review the current statu...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2013.12.011
更新日期:2014-03-01 00:00:00
abstract:OBJECTIVES:To characterize a novel deletion of exon 3 of CFTR gene and to evaluate the implications in Cystic Fibrosis (CF) care and genetic counseling. DESIGN AND METHODS:We performed a wide mutational analysis of CFTR gene, using reverse dot blot, Multiplex Ligation-dependent Probe Amplification (MLPA) assay and Rea...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2011.03.140
更新日期:2011-07-01 00:00:00