Abstract：
:Cell growth and differentiation are usually antagonistic. Proteins of the basic helix-loop-helix (bHLH) family bind DNA and play important roles in the differentiation of specific cell types. Id proteins heterodimerize with bHLH transcription factors, blocking their activation of lineage-specific gene expression and thereby inhibiting cellular differentiation. To examine the effect of Id-2 on cell proliferation, we overexpressed Id-2 in the human osteosarcoma cell line U2OS. Id-2 expression in U2OS reduced the serum requirement for growth and stimulated cellular proliferation by shortening the doubling time and increasing the percentage of cells in S phase. We demonstrated that Id-2 expression was able to reverse the inhibition of cellular proliferation and the block in cell cycle progression mediated by the product of the retinoblastoma tumor suppressor gene pRB. This effect was not associated with changes in the state of pRb phosphorylation in transfected cells. In vitro, unphosphorylated pRb from cell lysates specifically bound Id-2 but was not able to bind a mutated form of Id-2 lacking the HLH domain that also did not antagonize the growth arrest by pRb. In vitro-synthesized pRb containing mutations within the E1A/large T-binding pocket did not bind Id-2. However, wild-type pRb was able to bind to a region of Id-2 corresponding to only the HLH domain. In vivo, a physical association between Id-2 and pRb was seen in cross-linked extracts from SAOS-2 cells transfected with Id-2 and pRb. Our data identify a role for Id-2 in the regulation of cellular proliferation and suggest that the interaction between Id-2 and pRB is a molecular pathway over which synchronous changes in growth and differentiation are mediated in vivo.
journal_name
Genes Devjournal_title
Genes & developmentauthors
Iavarone A,Garg P,Lasorella A,Hsu J,Israel MAdoi
10.1101/gad.8.11.1270subject
Has Abstractpub_date
1994-06-01 00:00:00pages
1270-84issue
11eissn
0890-9369issn
1549-5477journal_volume
8pub_type
杂志文章abstract：:The unique property of stem cells to self-renew suggests specific mechanisms that regulate their cell-cycle progression. In the present study, we identify a novel protein, nucleostemin, found in the nucleoli of CNS stem cells, embryonic stem cells, and several cancer cell lines and preferentially expressed by other st...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.55671
更新日期：2002-12-01 00:00:00
abstract：:Hypocretin (orexin; Hcrt)-containing neurons of the hypothalamus are essential for the normal regulation of sleep and wake behaviors and have been implicated in feeding, anxiety, depression, and reward. The absence of these neurons causes narcolepsy in humans and model organisms. However, little is known about the mol...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.207654.112
更新日期：2013-03-01 00:00:00
abstract：:The structurally related POU homeo domain proteins Pit-1 and Oct-2 activate pituitary- and lymphoid-specific transcription, respectively, by binding to similar AT-rich motifs in their target genes. In this study we identify bases critical for recognition and activation by Pit-1 and examine how small differences in Pit...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.4.1.43
更新日期：1990-01-01 00:00:00
abstract：:In Drosophila, the development of the compound eye depends on the movement of a morphogenetic furrow (MF) from the posterior (P) to the anterior (A) of the eye imaginal disc. We define several subdomains along the A-P axis of the eye disc that express distinct combinations of transcription factors. One subdomain, ante...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1009002
更新日期：2002-09-15 00:00:00
abstract：:The nonsense-mediated mRNA decay (NMD) pathway selectively degrades mRNAs harboring premature termination codons (PTCs). Seven genes (smg-1-7, for suppressor with morphological effect on genitalia) that are essential for NMD were originally identified in the nematode Caenorhabditis elegans, and orthologs of these gene...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.417707
更新日期：2007-05-01 00:00:00
abstract：:Alu repetitive sequences are the most abundant short interspersed DNA elements in the human genome. Full-length Alu elements are composed of two tandem sequence monomers, the left and right Alu arms, both derived from the 7SL signal recognition particle RNA. Since Alu elements are common in protein-coding genes, they ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.197467.112
更新日期：2012-09-01 00:00:00
abstract：:The recombination site dif is the target on the Escherichia coli chromosome of the site-specific recombinases XerC and XerD. The dif/XerC-D system plays a role during the cell cycle, probably by favoring sister chromosome monomerization or separation. A phenomenon of regional control over dif activity, also analyzed i...
journal_title：Genes & development
pub_type： 评论,杂志文章
doi：10.1101/gad.10.9.1152
更新日期：1996-05-01 00:00:00
abstract：:To find new genes that influence liver lipid mass, we performed a genetic screen for zebrafish mutants with hepatic steatosis, a pathological accumulation of fat. The red moon (rmn) mutant develops hepatic steatosis as maternally deposited yolk is depleted. Conversely, hepatic steatosis is suppressed in rmn mutants by...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.180968.111
更新日期：2012-02-01 00:00:00
abstract：:We have investigated the role of erythroid Kruppel-like factor (EKLF) in expression of the human beta-globin genes in compound EKLF knockout/human beta-locus transgenic mice. EKLF affects only the adult mouse beta-globin genes in homozygous knockout mice; heterozygous mice are unaffected. Here we show that EKLF knocko...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.22.2894
更新日期：1996-11-15 00:00:00
abstract：:Actin is associated with all three nuclear RNA polymerases and acts in concert with nuclear myosin I (NM1) to drive transcription. Practically nothing is known regarding the state of actin and the functional interplay of actin and NM1 in transcription. Here we show that actin and NM1 act in concert to promote RNA poly...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.455908
更新日期：2008-02-01 00:00:00
abstract：:The ECS (Elongin B/C-Cul2/Cul5-SOCS-box protein) complex is a member of a family of ubiquitin ligases that share a Cullin-Rbx module. SOCS-box proteins recruit substrates to the ECS complex and are linked to Cullin-Rbx via Elongin B/C. VHL has been implicated as a SOCS-box protein, but lacks a C-terminal sequence (dow...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1252404
更新日期：2004-12-15 00:00:00
abstract：:The product of the nuclear MRS2 gene, Mrs2p, is the only candidate splicing factor essential for all group II introns in mitochondria of the yeast Saccharomyces cerevisiae. It has been shown to be an integral protein of the inner mitochondrial membrane, structurally and functionally related to the bacterial CorA Mg(2+...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.201301
更新日期：2001-09-01 00:00:00
abstract：:Transcription of bacteriophage N4 middle genes is carried out by a phage-coded, heterodimeric RNA polymerase (N4 RNAPII), which belongs to the family of T7-like RNA polymerases. In contrast to phage T7-RNAP, N4 RNAPII displays no activity on double-stranded templates and low activity on single-stranded templates. In v...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1121403
更新日期：2003-09-15 00:00:00
abstract：:Small noncoding RNAs, including small interfering RNAs (siRNAs) and micro RNAs (miRNAs) of approximately 21 nucleotides (nt) in length, have emerged as potent regulators of gene expression at both transcriptional and post-transcriptional levels in diverse organisms. Here we report the identification of a novel class o...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1434406
更新日期：2006-07-01 00:00:00
abstract：:Repression of a-cell specific gene expression in yeast alpha cells requires MAT alpha 2 and MCM1, as well as two global repressors, SSN6 and TUP1. Previous studies demonstrated that nucleosomes positioned adjacent to the alpha 2/MCM1 operator in alpha cells directly contribute to repression. To investigate the possibi...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.8.12.1400
更新日期：1994-06-15 00:00:00
abstract：:The tobacco mitogen-activated protein kinase (MAPK) cascade, which includes MAPK NRK1/NTF6, positively regulates expansion of the cytokinetic machinery known as the phragmoplast, which is followed by the synthesis of cell plates for completion of cell division. However, molecular events lying between the MAPK and phra...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1408106
更新日期：2006-04-15 00:00:00
abstract：:The yeast SAGA (Spt-Ada-Gcn5-acetyltransferase) coactivator complex exerts functions in gene expression, including activator interaction, histone acetylation, histone deubiquitination, mRNA export, chromatin recognition, and regulation of the basal transcription machinery. These diverse functions involve distinct modu...
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.184705.111
更新日期：2012-03-15 00:00:00
abstract：:Bone morphogenic proteins 2 and 4 (BMP2 and BMP4) inhibit proliferation and induce differentiation of cerebellar granule neuron progenitors (GNPs) and primary GNP-like medulloblastoma (MB) cells. This occurs through rapid proteasome-mediated degradation of Math1 (Atoh1), a transcription factor expressed in proliferati...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1636408
更新日期：2008-03-15 00:00:00
abstract：:An RNA-binding protein gene (rbp1) from Drosophila melanogaster, encoding an RNA recognition motif and an Arg-Ser rich (RS) domain, has been characterized. The predicted amino acid sequence of rbp1 is similar to those of the human splicing factor ASF/SF2, the Drosophila nuclear phosphoprotein SRp55, and the Drosophila...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.6.12b.2569
更新日期：1992-12-01 00:00:00
abstract：:AMP-activated protein kinase (AMPK) is a sensor of energy status that maintains cellular energy homeostasis. It arose very early during eukaryotic evolution, and its ancestral role may have been in the response to starvation. Recent work shows that the kinase is activated by increases not only in AMP, but also in ADP....
journal_title：Genes & development
pub_type： 杂志文章,评审
doi：10.1101/gad.17420111
更新日期：2011-09-15 00:00:00
abstract：:U6 small nuclear RNA is one of the spliceosomal RNAs involved in pre-mRNA splicing. In the fission yeast Schizosaccharomyces pombe, the U6 RNA gene was found to have an intron similar to a nuclear pre-mRNA intron, and it was proposed that the U6 intron might be inserted erroneously during pre-mRNA splicing. Using the ...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.5.6.1022
更新日期：1991-06-01 00:00:00
abstract：:The ascidian tadpole represents the most simplified chordate body plan. It contains a notochord composed of just 40 cells, but as in vertebrates Brachyury is essential for notochord differentiation. Here, we show that the misexpression of the Brachyury gene (Ci-Bra) of Ciona intestinalis is sufficient to transform end...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.13.12.1519
更新日期：1999-06-15 00:00:00
abstract：:We have shown that Wnt signals are necessary and sufficient for neural crest cells to adopt pigment cell fates. nacre, a zebrafish homolog of MITF, is required for pigment cell differentiation. We isolated a promoter region of nacre that contains Tcf/Lef binding sites, which can mediate Wnt responsiveness. This promot...
journal_title：Genes & development
pub_type： 杂志文章
doi：
更新日期：2000-01-15 00:00:00
abstract：:The Drosophila brain tumor (brat) gene encodes a member of the conserved NHL family of proteins, which appear to regulate differentiation and growth in a variety of organisms. One of the founding family members, Caenorhabditis elegans LIN-41, is thought to control posttranscriptional gene expression. However, the mech...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.870801
更新日期：2001-03-15 00:00:00
abstract：:A prominent feature of cell differentiation is the initiation and maintenance of an irreversible cell cycle arrest with the complex involvement of the retinoblastoma (RB) family (RB, p130, p107). We have isolated the HBP1 transcriptional repressor as a potential target of the RB family in differentiated cells. By homo...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.11.3.383
更新日期：1997-02-01 00:00:00
abstract：:Although Apc is well characterized as a tumor-suppressor gene in the intestine, the precise mechanism of this suppression remains to be defined. Using a novel inducible Ahcre transgenic line in conjunction with a loxP-flanked Apc allele we, show that loss of Apc acutely activates Wnt signaling through the nuclear accu...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.287404
更新日期：2004-06-15 00:00:00
abstract：:Wingless (Wg) is an important signaling molecule in the development of Drosophila, but little is known about its signal transduction pathway. Genetic evidence indicates that another segment polarity gene, dishevelled (dsh) is required for Wg signaling. We have recently developed a cell culture system for Wg protein ac...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.9.9.1087
更新日期：1995-05-01 00:00:00
abstract：:The Arabidopsis APETALA3 (AP3) and PISTILLATA (PI) proteins are thought to act as transcription factors and are required for specifying floral organ identities. To define the nuclear localization signals within these proteins, we generated translational fusions of the coding regions of AP3 and PI to the bacterial uidA...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.10.14.1812
更新日期：1996-07-15 00:00:00
abstract：:Macrophage activation by the proinflammatory cytokine interferon-gamma (IFN-gamma) is a critical component of the host innate response to bacterial pathogenesis. However, the precise nature of the IFN-gamma-induced activation pathway is not known. Here we show using genome-wide expression and chromatin-binding profili...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1553007
更新日期：2007-08-01 00:00:00
abstract：:To define the functional pathways regulating epithelial cell migration, we performed a genome-wide RNAi screen using 55,000 pooled lentiviral shRNAs targeting ∼11,000 genes, selecting for transduced cells with increased motility. A stringent validation protocol generated a set of 31 genes representing diverse pathways...
journal_title：Genes & development
pub_type： 杂志文章
doi：10.1101/gad.1989110
更新日期：2010-12-01 00:00:00
