当前位置： SCI文献检索 > GENES & DEVELOPMENT期刊下所有文献 > Mitochondrial Mg(2+) homeostasis is critical for group II intron splicing in vivo.

Mitochondrial Mg(2+) homeostasis is critical for group II intron splicing in vivo.

Abstract：

:The product of the nuclear MRS2 gene, Mrs2p, is the only candidate splicing factor essential for all group II introns in mitochondria of the yeast Saccharomyces cerevisiae. It has been shown to be an integral protein of the inner mitochondrial membrane, structurally and functionally related to the bacterial CorA Mg(2+) transporter. Here we show that mutant alleles of the MRS2 gene as well as overexpression of this gene both increase intramitochondrial Mg(2+) concentrations and compensate for splicing defects of group II introns in mit(-) mutants M1301 and B-loop. Yet, covariation of Mg(2+) concentrations and splicing is similarly seen when some other genes affecting mitochondrial Mg(2+) concentrations are overexpressed in an mrs2Delta mutant, indicating that not the Mrs2 protein per se but certain Mg(2+) concentrations are essential for group II intron splicing. This critical role of Mg(2+) concentrations for splicing is further documented by our observation that pre-mRNAs, accumulated in mitochondria isolated from mutants, efficiently undergo splicing in organello when these mitochondria are incubated in the presence of 10 mM external Mg(2+) (mit(-) M1301) and an ionophore (mrs2Delta). This finding of an exceptional sensitivity of group II intron splicing toward Mg(2+) concentrations in vivo is unprecedented and raises the question of the role of Mg(2+) in other RNA-catalyzed reactions in vivo. It explains finally why protein factors modulating Mg(2+) homeostasis had been identified in genetic screens for bona fide RNA splicing factors.

journal_name

Genes Dev

journal_title

Genes & development

authors

Gregan J,Kolisek M,Schweyen RJ

doi

10.1101/gad.201301

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

2229-37

issue

17

eissn

0890-9369

issn

1549-5477

journal_volume

15

pub_type

杂志文章

相关文献

GENES & DEVELOPMENT文献大全
  • Constitutive mTORC1 activation by a herpesvirus Akt surrogate stimulates mRNA translation and viral replication.

    abstract：:All viruses require cellular ribosomes to translate their mRNAs. Viruses producing methyl-7 (m⁷) GTP-capped mRNAs, like Herpes Simplex Virus-1 (HSV-1), stimulate cap-dependent translation by activating mTORC1 to inhibit the translational repressor 4E-binding protein 1 (4E-BP1). Here, we establish that the HSV-1 kinase...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1978310

    authors： Chuluunbaatar U,Roller R,Feldman ME,Brown S,Shokat KM,Mohr I

    更新日期：2010-12-01 00:00:00

  • A novel class of small RNAs in mouse spermatogenic cells.

    abstract：:Small noncoding RNAs, including small interfering RNAs (siRNAs) and micro RNAs (miRNAs) of approximately 21 nucleotides (nt) in length, have emerged as potent regulators of gene expression at both transcriptional and post-transcriptional levels in diverse organisms. Here we report the identification of a novel class o...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1434406

    authors： Grivna ST,Beyret E,Wang Z,Lin H

    更新日期：2006-07-01 00:00:00

  • Mouse model for the lysosomal disorder galactosialidosis and correction of the phenotype with overexpressing erythroid precursor cells.

    abstract：:The lysosomal storage disorder galactosialidosis results from a primary deficiency of the protective protein/cathepsin A (PPCA), which in turn affects the activities of beta-galactosidase and neuraminidase. Mice homozygous for a null mutation at the PPCA locus present with signs of the disease shortly after birth and ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.9.21.2623

    authors： Zhou XY,Morreau H,Rottier R,Davis D,Bonten E,Gillemans N,Wenger D,Grosveld FG,Doherty P,Suzuki K,Grosveld GC,d'Azzo A

    更新日期：1995-11-01 00:00:00

  • Factors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain.

    abstract：:TFIID is a multisubunit protein containing the TATA box-binding polypeptide (TBP) and associated factors (TFIID-TAFs) required for activated transcription by RNA polymerase II. TBPs from different eukaryotes contain a highly conserved carboxy-terminal domain and very divergent amino-terminal domains. Earlier studies p...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.7.2.180

    authors： Zhou Q,Boyer TG,Berk AJ

    更新日期：1993-02-01 00:00:00

  • A nucleoprotein complex mediates the integration of retroviral DNA.

    abstract：:The integration of viral DNA into the host genome is an essential step in the retrovirus life cycle. To understand this process better, we have examined the native state of viral DNA in cells acutely infected by murine leukemia virus (MLV), using both a physical assay for viral DNA and a functional assay for integrati...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.3.4.469

    authors： Bowerman B,Brown PO,Bishop JM,Varmus HE

    更新日期：1989-04-01 00:00:00

  • Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay.

    abstract：:Glucocorticoid (GC) receptor (GR) has been shown recently to bind a subset of mRNAs and elicit rapid mRNA degradation. However, the molecular details of GR-mediated mRNA decay (GMD) remain unclear. Here, we demonstrate that GMD triggers rapid degradation of target mRNAs in a translation-independent and exon junction c...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.286484.116

    authors： Park OH,Park J,Yu M,An HT,Ko J,Kim YK

    更新日期：2016-09-15 00:00:00

  • CSA-dependent degradation of CSB by the ubiquitin-proteasome pathway establishes a link between complementation factors of the Cockayne syndrome.

    abstract：:Mutations in the CSA or CSB complementation genes cause the Cockayne syndrome, a severe genetic disorder that results in patients' death in early adulthood. CSA and CSB act in a transcription-coupled repair (TCR) pathway, but their functional relationship is not understood. We have previously shown that CSA is a subun...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.378206

    authors： Groisman R,Kuraoka I,Chevallier O,Gaye N,Magnaldo T,Tanaka K,Kisselev AF,Harel-Bellan A,Nakatani Y

    更新日期：2006-06-01 00:00:00

  • A mechanism for the exclusion of low-fidelity human Y-family DNA polymerases from base excision repair.

    abstract：:The human Y-family DNA polymerases, Poliota, Poleta, and Polkappa, function in promoting replication through DNA lesions. However, because of their low fidelity, any involvement of these polymerases in DNA synthesis during base excision repair (BER) would be highly mutagenic. Mechanisms, therefore, must exist to exclu...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1146103

    authors： Haracska L,Prakash L,Prakash S

    更新日期：2003-11-15 00:00:00

  • Suppression of cap-dependent translation in mitosis.

    abstract：:Cap-dependent translation is mediated by eIF4F, a protein complex composed of three subunits as follows: eIF4E, which recognizes the mRNA 5' cap structure; eIF4A, an RNA-helicase; and eIF4G, a scaffolding protein that binds eIF4E, eIF4A, and the eIF4E-kinase Mnk1 simultaneously. eIF4E is hypophosphorylated and cap-dep...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.889201

    authors： Pyronnet S,Dostie J,Sonenberg N

    更新日期：2001-08-15 00:00:00

  • Epigenetic effects on yeast transcription caused by mutations in an actin-related protein present in the nucleus.

    abstract：:Gene inactivation can result when a delta element of the Ty1 transposon inserted into the yeast HIS4 promoter (his4-912delta) alters the transcription initiation site. Previous work has identified mutations that suppress this transcriptional defect by restoring the transcription start site to the native position, and ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.10.5.604

    authors： Jiang YW,Stillman DJ

    更新日期：1996-03-01 00:00:00

  • Loss of C. elegans BBS-7 and BBS-8 protein function results in cilia defects and compromised intraflagellar transport.

    abstract：:Bardet-Biedl syndrome (BBS) is a genetically heterogeneous developmental disorder whose molecular basis is largely unknown. Here, we show that mutations in the Caenorhabditis elegans bbs-7 and bbs-8 genes cause structural and functional defects in cilia. C. elegans BBS proteins localize predominantly at the base of ci...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1194004

    authors： Blacque OE,Reardon MJ,Li C,McCarthy J,Mahjoub MR,Ansley SJ,Badano JL,Mah AK,Beales PL,Davidson WS,Johnsen RC,Audeh M,Plasterk RH,Baillie DL,Katsanis N,Quarmby LM,Wicks SR,Leroux MR

    更新日期：2004-07-01 00:00:00

  • cAMP stimulates the C/EBP-related transcription factor rNFIL-6 to trans-locate to the nucleus and induce c-fos transcription.

    abstract：:The c-fos serum response element (SRE) is a multifunctional regulatory region of the c-fos promoter that responds to a variety of inducers. Recently, we have demonstrated that the SRE binds the C/EBP-related transcription factor rat NFIL-6 (rNFIL-6). In this study we show that rNFIL-6 is regulated by the cAMP second m...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.5.10.1754

    authors： Metz R,Ziff E

    更新日期：1991-10-01 00:00:00

  • A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group alpha(1,3/1,4)fucosyltransferase.

    abstract：:The stage-specific embryonic antigen SSEA-1 is a cell-surface oligosaccharide molecule expressed with temporal precision during the murine preimplantation period and implicated in adhesive events involving the process of compaction. We used a mammalian transient expression system to isolate a cloned human cDNA that de...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.4.8.1288

    authors： Kukowska-Latallo JF,Larsen RD,Nair RP,Lowe JB

    更新日期：1990-08-01 00:00:00

  • Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation.

    abstract：:A novel histone methyltransferase, termed Set9, was isolated from human cells. Set9 contains a SET domain, but lacks the pre- and post-SET domains. Set9 methylates specifically lysine 4 (K4) of histone H3 (H3-K4) and potentiates transcription activation. The histone H3 tail interacts specifically with the histone deac...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.967202

    authors： Nishioka K,Chuikov S,Sarma K,Erdjument-Bromage H,Allis CD,Tempst P,Reinberg D

    更新日期：2002-02-15 00:00:00

  • Expression of an activated ras gene causes developmental abnormalities in transgenic Drosophila melanogaster.

    abstract：:We describe the developmental effects of the expression of a normal and mutated Drosophila ras gene ras2 in transgenic Drosophila. A Gly14----Val14 mutant (Gly14 in ras2 is equivalent to Gly12 in mammalian ras proteins) was constructed in ras2 by site-directed mutagenesis. Inducible constructs of ras2 and ras2Val14 we...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.2.5.567

    authors： Bishop JG 3rd,Corces VG

    更新日期：1988-05-01 00:00:00

  • flbD encodes a Myb-like DNA-binding protein that coordinates initiation of Aspergillus nidulans conidiophore development.

    abstract：:The timing of asexual fruiting body formation during Aspergillus nidulans colony development is precisely regulated so that conidiophores are typically produced 1-2 mm behind the growing edge of the colony. Mutations in any of four A. nidulans genes, flbB, flbC, flbD, or flbE, result in colonies that are delayed at le...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.9.4.491

    authors： Wieser J,Adams TH

    更新日期：1995-02-15 00:00:00

  • A nonproteolytic function of the proteasome is required for the dissociation of Cdc2 and cyclin B at the end of M phase.

    abstract：:Inactivation of cyclin B-Cdc2 kinase at the exit from M phase depends on the specific proteolysis of the cyclin B subunit, whereas the Cdc2 subunit remains present at nearly constant levels throughout the cell cycle. It is unknown how Cdc2 escapes degradation when cyclin B is destroyed. In Xenopus egg extracts that re...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.823200

    authors： Nishiyama A,Tachibana K,Igarashi Y,Yasuda H,Tanahashi N,Tanaka K,Ohsumi K,Kishimoto T

    更新日期：2000-09-15 00:00:00

  • Suprabasal expression of a dominant-negative RXR alpha mutant in transgenic mouse epidermis impairs regulation of gene transcription and basal keratinocyte proliferation by RAR-selective retinoids.

    abstract：:To determine whether 9-cis retinoic acid receptors (RXRs) regulate the biological activity of all-trans retinoic acid (tRA) and its receptors (RARs) in skin, we have targeted a dominant-negative RXR alpha (dnRXR alpha) lacking transactivation function AF-2 to differentiated suprabasal keratinocytes in the epidermis of...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.11.1.59

    authors： Feng X,Peng ZH,Di W,Li XY,Rochette-Egly C,Chambon P,Voorhees JJ,Xiao JH

    更新日期：1997-01-01 00:00:00

  • Protein kinase C-zeta reverts v-raf transformation of NIH-3T3 cells.

    abstract：:We have identified protein kinase C-zeta (PKC-zeta) as a novel suppressor of neoplastic transformation caused by the v-raf oncogene. PKC-zeta overexpression drastically retards proliferation, abolishes anchorage-independent growth, and reverts the morphological transformation of v-raf-transformed NIH-3T3 cells. The mo...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.10.12.1455

    authors： Kieser A,Seitz T,Adler HS,Coffer P,Kremmer E,Crespo P,Gutkind JS,Henderson DW,Mushinski JF,Kolch W,Mischak H

    更新日期：1996-06-15 00:00:00

  • An ERK2 docking site in the Pointed domain distinguishes a subset of ETS transcription factors.

    abstract：:The ETS transcription factors perform distinct biological functions despite conserving a highly similar DNA-binding domain. One distinguishing property of a subset of ETS proteins is a conserved region of 80 amino acids termed the Pointed (PNT) domain. Using enzyme kinetics we determined that the Ets-1 PNT domain cont...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.950902

    authors： Seidel JJ,Graves BJ

    更新日期：2002-01-01 00:00:00

  • SWI/SNF regulates a transcriptional program that induces senescence to prevent liver cancer.

    abstract：:Oncogene-induced senescence (OIS) is a potent tumor suppressor mechanism. To identify senescence regulators relevant to cancer, we screened an shRNA library targeting genes deleted in hepatocellular carcinoma (HCC). Here, we describe how knockdown of the SWI/SNF component ARID1B prevents OIS and cooperates with RAS to...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.286112.116

    authors： Tordella L,Khan S,Hohmeyer A,Banito A,Klotz S,Raguz S,Martin N,Dhamarlingam G,Carroll T,González Meljem JM,Deswal S,Martínez-Barbera JP,García-Escudero R,Zuber J,Zender L,Gil J

    更新日期：2016-10-01 00:00:00

  • p53: master of life, death, and the epigenome.

    abstract：:Long understood as a bona fide tumor suppressor that safeguards the integrity of the genome via regulating numerous cellular outcomes, p53 may also exert its decisive and versatile functions by controlling DNA methylation. In this issue of Genes & Development, Tovy and colleagues (pp. 959-972) report that, in naïve mo...

    journal_title：Genes & development

    pub_type： 评论,杂志文章,评审

    doi：10.1101/gad.302364.117

    authors： Laptenko O,Prives C

    更新日期：2017-05-15 00:00:00

  • The excess microsporocytes1 gene encodes a putative leucine-rich repeat receptor protein kinase that controls somatic and reproductive cell fates in the Arabidopsis anther.

    abstract：:Cell differentiation is essential for the development of multicellular organisms. In flowering plants, the haploid male gametophytes (pollen grains) are generated in the anther from reproductive cells called microsporocytes. Several types of somatic cells ensure successful pollen development, and thus reproduction. Ho...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.997902

    authors： Zhao DZ,Wang GF,Speal B,Ma H

    更新日期：2002-08-01 00:00:00

  • Restriction of the activity of the recombination site dif to a small zone of the Escherichia coli chromosome.

    abstract：:The recombination site dif is the target on the Escherichia coli chromosome of the site-specific recombinases XerC and XerD. The dif/XerC-D system plays a role during the cell cycle, probably by favoring sister chromosome monomerization or separation. A phenomenon of regional control over dif activity, also analyzed i...

    journal_title：Genes & development

    pub_type： 评论,杂志文章

    doi：10.1101/gad.10.9.1152

    authors： Cornet F,Louarn J,Patte J,Louarn JM

    更新日期：1996-05-01 00:00:00

  • IKK1-deficient mice exhibit abnormal development of skin and skeleton.

    abstract：:IkappaB kinases (IKKs) IKK1 and IKK2 are two putative IkappaBalpha kinases involved in NF-kappaB activation. To examine the in vivo functions of IKK1, we generated IKK1-deficient mice. The mutant mice are perinatally lethal and exhibit a wide range of developmental defects. Newborn mutant mice have shiny, taut, and st...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.13.10.1322

    authors： Li Q,Lu Q,Hwang JY,Büscher D,Lee KF,Izpisua-Belmonte JC,Verma IM

    更新日期：1999-05-15 00:00:00

  • Cdk1-Clb4 controls the interaction of astral microtubule plus ends with subdomains of the daughter cell cortex.

    abstract：:As in many polarized cells, spindle alignment in yeast is essential and cell cycle regulated. A key step that governs spindle alignment is the selective binding of the Kar9 protein to only one of the two spindle pole bodies (SPBs). It has been suggested that cyclin-dependent kinase Cdc28, in complex with cyclin Clb4, ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.298704

    authors： Maekawa H,Schiebel E

    更新日期：2004-07-15 00:00:00

  • Disruption of the HNF-4 gene, expressed in visceral endoderm, leads to cell death in embryonic ectoderm and impaired gastrulation of mouse embryos.

    abstract：:Expression of HNF-4, a transcription factor in the steroid hormone receptor superfamily, is detected only in the visceral endoderm of mouse embryos during gastrulation and is expressed in certain embryonic tissues from 8.5 days of gestation. To examine the role of HNF-4 during embryonic development, we disrupted the g...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.8.20.2466

    authors： Chen WS,Manova K,Weinstein DC,Duncan SA,Plump AS,Prezioso VR,Bachvarova RF,Darnell JE Jr

    更新日期：1994-10-15 00:00:00

  • PR72, a novel regulator of Wnt signaling required for Naked cuticle function.

    abstract：:The Wnt signaling cascade is a central regulator of cell fate determination during embryonic development, whose deregulation contributes to oncogenesis. Naked cuticle is the first Wnt-induced antagonist found in this pathway, establishing a negative-feedback loop that limits the Wnt signal required for early segmentat...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.328905

    authors： Creyghton MP,Roël G,Eichhorn PJ,Hijmans EM,Maurer I,Destrée O,Bernards R

    更新日期：2005-02-01 00:00:00

  • Cyclic GMP-dependent protein kinase II is a molecular switch from proliferation to hypertrophic differentiation of chondrocytes.

    abstract：:The Komeda miniature rat Ishikawa (KMI) is a naturally occurring mutant caused by an autosomal recessive mutation mri, which exhibits longitudinal growth retardation. Here we identified the mri mutation as a deletion in the rat gene encoding cGMP-dependent protein kinase type II (cGKII). KMIs showed an expanded growth...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.1224204

    authors： Chikuda H,Kugimiya F,Hoshi K,Ikeda T,Ogasawara T,Shimoaka T,Kawano H,Kamekura S,Tsuchida A,Yokoi N,Nakamura K,Komeda K,Chung UI,Kawaguchi H

    更新日期：2004-10-01 00:00:00

  • Rev-erbα and Rev-erbβ coordinately protect the circadian clock and normal metabolic function.

    abstract：:The nuclear receptor Rev-erbα regulates circadian rhythm and metabolism, but its effects are modest and it has been considered to be a secondary regulator of the cell-autonomous clock. Here we report that depletion of Rev-erbα together with closely related Rev-erbβ has dramatic effects on the cell-autonomous clock as ...

    journal_title：Genes & development

    pub_type： 杂志文章

    doi：10.1101/gad.186858.112

    authors： Bugge A,Feng D,Everett LJ,Briggs ER,Mullican SE,Wang F,Jager J,Lazar MA

    更新日期：2012-04-01 00:00:00