CSA-dependent degradation of CSB by the ubiquitin-proteasome pathway establishes a link between complementation factors of the Cockayne syndrome.

abstract：:To define the functional pathways regulating epithelial cell migration, we performed a genome-wide RNAi screen using 55,000 pooled lentiviral shRNAs targeting ∼11,000 genes, selecting for transduced cells with increased motility. A stringent validation protocol generated a set of 31 genes representing diverse pathways...

journal_title：Genes & development

authors： Smolen GA,Zhang J,Zubrowski MJ,Edelman EJ,Luo B,Yu M,Ng LW,Scherber CM,Schott BJ,Ramaswamy S,Irimia D,Root DE,Haber DA

更新日期：2010-12-01 00:00:00

abstract：:In the metazoan germline, piwi proteins and associated piwi-interacting RNAs (piRNAs) provide a defense system against the expression of transposable elements. In the cytoplasm, piRNA sequences guide piwi complexes to destroy complementary transposon transcripts by endonucleolytic cleavage. However, some piwi family m...

journal_title：Genes & development

authors： Le Thomas A,Rogers AK,Webster A,Marinov GK,Liao SE,Perkins EM,Hur JK,Aravin AA,Tóth KF

更新日期：2013-02-15 00:00:00

abstract：:Histone post-translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. While significant progress has been made characterizing individual effector domains, the role of paired domains and how they functi...

journal_title：Genes & development

authors： Rothbart SB,Dickson BM,Ong MS,Krajewski K,Houliston S,Kireev DB,Arrowsmith CH,Strahl BD

更新日期：2013-06-01 00:00:00

abstract：:Metastasis accounts for the vast majority of cancer-related deaths, yet the molecular mechanisms that drive metastatic spread remain poorly understood. Here we report that Tks5, which has been linked to the formation of proteolytic cellular protrusions known as invadopodia, undergoes an isoform switch during metastati...

journal_title：Genes & development

authors： Li CM,Chen G,Dayton TL,Kim-Kiselak C,Hoersch S,Whittaker CA,Bronson RT,Beer DG,Winslow MM,Jacks T

更新日期：2013-07-15 00:00:00

abstract：:Cell cycle transitions are often accompanied by the degradation of regulatory molecules. Targeting proteins to the proteasome for degradation is accomplished by the covalent addition of ubiquitin chains. The specificity of this pathway is largely dictated by a set of enzymes called ubiquitin ligases (or E3s). The anap...

journal_title：Genes & development

authors： Thornton BR,Toczyski DP

更新日期：2006-11-15 00:00:00

abstract：:Inactivation of cyclin B-Cdc2 kinase at the exit from M phase depends on the specific proteolysis of the cyclin B subunit, whereas the Cdc2 subunit remains present at nearly constant levels throughout the cell cycle. It is unknown how Cdc2 escapes degradation when cyclin B is destroyed. In Xenopus egg extracts that re...

journal_title：Genes & development

authors： Nishiyama A,Tachibana K,Igarashi Y,Yasuda H,Tanahashi N,Tanaka K,Ohsumi K,Kishimoto T

更新日期：2000-09-15 00:00:00

abstract：:A conditional block to transcriptional elongation is an important mechanism for regulating c-myc gene expression. This elongation block within the first c-myc exon was defined originally in mammalian cells by nuclear run-on transcription analyses. Subsequent oocyte injection and in vitro transcription analyses suggest...

journal_title：Genes & development

authors： Krumm A,Meulia T,Brunvand M,Groudine M

更新日期：1992-11-01 00:00:00

abstract：:All multisubunit DNA-dependent RNA polymerases (RNAP) are zinc metalloenzymes, and at least two zinc atoms are present per enzyme molecule. RNAP residues involved in zinc binding and the functional role of zinc ions in the transcription mechanism or RNAP structure are unknown. Here, we locate four cysteine residues in...

journal_title：Genes & development

authors： Markov D,Naryshkina T,Mustaev A,Severinov K

更新日期：1999-09-15 00:00:00

abstract：:Transcriptional profiling is a powerful approach for understanding development and disease. Current cell type-specific RNA purification methods have limitations, including cell dissociation trauma or inability to identify all RNA species. Here, we describe "mouse thiouracil (TU) tagging," a genetic and chemical inters...

journal_title：Genes & development

authors： Gay L,Miller MR,Ventura PB,Devasthali V,Vue Z,Thompson HL,Temple S,Zong H,Cleary MD,Stankunas K,Doe CQ

更新日期：2013-01-01 00:00:00

abstract：:The Drosophila somatic sex-determination regulatory pathway has been well studied, but little is known about the target genes that it ultimately controls. In a differential screen for sex-specific transcripts expressed in fly heads, we identified a highly male-enriched transcript encoding Takeout, a protein related to...

journal_title：Genes & development

authors： Dauwalder B,Tsujimoto S,Moss J,Mattox W

更新日期：2002-11-15 00:00:00

abstract：:Cellular senescence limits proliferation of potentially detrimental cells, preventing tumorigenesis and restricting tissue damage. However, the function of senescence in nonpathological conditions is unknown. We found that the human placental syncytiotrophoblast exhibited the phenotype and expressed molecular markers ...

journal_title：Genes & development

authors： Chuprin A,Gal H,Biron-Shental T,Biran A,Amiel A,Rozenblatt S,Krizhanovsky V

更新日期：2013-11-01 00:00:00

abstract：:The Arabidopsis APETALA3 (AP3) and PISTILLATA (PI) proteins are thought to act as transcription factors and are required for specifying floral organ identities. To define the nuclear localization signals within these proteins, we generated translational fusions of the coding regions of AP3 and PI to the bacterial uidA...

journal_title：Genes & development

authors： McGonigle B,Bouhidel K,Irish VF

更新日期：1996-07-15 00:00:00

abstract：:Genomic imprinting, an inherently epigenetic phenomenon defined by parent of origin-dependent gene expression, is observed in mammals and flowering plants. Genome-scale surveys of imprinted expression and the underlying differential epigenetic marks have led to the discovery of hundreds of imprinted plant genes and co...

journal_title：Genes & development

authors： Rodrigues JA,Zilberman D

更新日期：2015-12-15 00:00:00

abstract：:Mice mutant for the bHLH gene Hes1, which is known to keep cells in a proliferative state, mostly lack thymus. Transfer of Hes1-null fetal liver cells into RAG2-null host mice normally reconstitutes B cells but fails to generate mature T cells in the thymus. In the reconstituted thymus, T cell differentiation is arres...

journal_title：Genes & development

authors： Tomita K,Hattori M,Nakamura E,Nakanishi S,Minato N,Kageyama R

更新日期：1999-05-01 00:00:00

abstract：:We hypothesized that basic helix-loop-helix (bHLH) MIST1 (BHLHA15) is a "scaling factor" that universally establishes secretory morphology in cells that perform regulated secretion. Here, we show that targeted deletion of MIST1 caused dismantling of the secretory apparatus of diverse exocrine cells. Parietal cells (PC...

journal_title：Genes & development

authors： Lo HG,Jin RU,Sibbel G,Liu D,Karki A,Joens MS,Madison BB,Zhang B,Blanc V,Fitzpatrick JA,Davidson NO,Konieczny SF,Mills JC

更新日期：2017-01-15 00:00:00

abstract：:Integrin receptors for extracellular matrix are critical for cell motility, but the signals that determine when to stop are not known. Analysis of distal tip cell (DTC) migration during gonadogenesis in Caenorhabditis elegans has revealed the importance of transcription factor vab-3/Pax6 in regulating the alpha integr...

journal_title：Genes & development

authors： Meighan CM,Schwarzbauer JE

更新日期：2007-07-01 00:00:00

abstract：:In petunia flowers, the loci an1, an2, and an11 control the pigmentation of the flower by stimulating the transcription of anthocyanin biosynthetic genes. The an1 and an2 locus were recently cloned and encode a basic helix-loop-helix (bHLH) and MYB-domain transcriptional activator, respectively. Here, we report the is...

journal_title：Genes & development

authors： de Vetten N,Quattrocchio F,Mol J,Koes R

更新日期：1997-06-01 00:00:00

abstract：:Mitotic duration is determined by activation of the anaphase-promoting complex/cyclosome (APC/C) bound to its coactivator, Cdc20. Kinetochores, the microtubule-interacting machines on chromosomes, restrain mitotic exit when not attached to spindle microtubules by generating a Cdc20-containing complex that inhibits the...

journal_title：Genes & development

authors： Kim T,Lara-Gonzalez P,Prevo B,Meitinger F,Cheerambathur DK,Oegema K,Desai A

更新日期：2017-06-01 00:00:00

abstract：:Two retrovirus promoter trap vectors (U3His and U3Neo) have been used to disrupt genes expressed in totipotent murine embryonal stem (ES) cells. Selection in L-histidinol or G418 produced clones in which the coding sequences for histidinol-dehydrogenase or neomycin-phosphotransferase were fused to sequences in or near...

journal_title：Genes & development

authors： von Melchner H,DeGregori JV,Rayburn H,Reddy S,Friedel C,Ruley HE

更新日期：1992-06-01 00:00:00

abstract：:We report a systematic mutational analysis of the helix-turn-helix motif (HTH) of the fushi tarazu (ftz) homeo domain (HD) of Drosophila. We started out by testing the function of chimeric ftz proteins containing either a part of the Sex combs reduced (Scr) or the muscle segment homeobox (msh) HDs. By complementation ...

journal_title：Genes & development

authors： Furukubo-Tokunaga K,Müller M,Affolter M,Pick L,Kloter U,Gehring WJ

更新日期：1992-06-01 00:00:00

abstract：:De novo chromatin assembly into regularly spaced nucleosomal arrays is essential for eukaryotic genome maintenance and inheritance. The Anti-Silencing Function 1 protein (ASF1) has been shown to be a histone chaperone, participating in DNA-replication-coupled nucleosome assembly. We show that mutations in the Drosophi...

journal_title：Genes & development

authors： Moshkin YM,Armstrong JA,Maeda RK,Tamkun JW,Verrijzer P,Kennison JA,Karch F

更新日期：2002-10-15 00:00:00

abstract：:The RNA polymerase II (RNAPII) C-terminal domain kinase, CDK12, regulates genome stability, expression of DNA repair genes, and cancer cell resistance to chemotherapy and immunotherapy. In addition to its role in mRNA biosynthesis of DNA repair genes, we show here that CDK12 phosphorylates the mRNA 5' cap-binding repr...

journal_title：Genes & development

authors： Choi SH,Martinez TF,Kim S,Donaldson C,Shokhirev MN,Saghatelian A,Jones KA

更新日期：2019-04-01 00:00:00

abstract：:High-fidelity chromosome transmission is fundamental in controlling the quality of the cell division cycle. The spindle pole-to-pole distance remains constant from metaphase to anaphase A. We show that fission yeast sister centromere-connecting proteins, Mis6 and Mis12, are required for correct spindle morphogenesis, ...

journal_title：Genes & development

authors： Goshima G,Saitoh S,Yanagida M

更新日期：1999-07-01 00:00:00

abstract：:Symbiotic nitrogen fixation involves the development of specialized organs called nodules within which plant photosynthates are exchanged for combined nitrogen of bacterial origin. To determine the importance of bacterial nitrogen metabolism in symbiosis, we have characterized a key regulator of this metabolism in Rhi...

journal_title：Genes & development

authors： Arcondéguy T,Huez I,Tillard P,Gangneux C,de Billy F,Gojon A,Truchet G,Kahn D

更新日期：1997-05-01 00:00:00

abstract：:Far1p is a bifunctional protein that is required to arrest the cell cycle and to establish cell polarity during yeast mating. Far1p is localized predominantly in the nucleus but accumulates in the cytoplasm in cells exposed to pheromones. Here we show that Far1p functions in both subcellular compartments: nuclear Far1...

journal_title：Genes & development

authors： Blondel M,Alepuz PM,Huang LS,Shaham S,Ammerer G,Peter M

更新日期：1999-09-01 00:00:00

abstract：:Sex determination is regulated by diverse pathways. Although upstream signals vary, a cysteine-rich DNA-binding domain (the DM motif) is conserved within downstream transcription factors of Drosophila melanogaster (Doublesex) and Caenorhabditis elegans (MAB-3). Vertebrate DM genes have likewise been identified and, re...

journal_title：Genes & development

authors： Zhu L,Wilken J,Phillips NB,Narendra U,Chan G,Stratton SM,Kent SB,Weiss MA

更新日期：2000-07-15 00:00:00

abstract：:The Hedgehog (Hh) family of secreted proteins controls many aspects of animal development. In Drosophila, Hh transduces its signal via Cubitus interruptus (Ci), a transcription factor present in two forms: a full-length activator and a carboxy-terminally truncated repressor that is derived from the full-length form by...

journal_title：Genes & development

authors： Wang G,Wang B,Jiang J

更新日期：1999-11-01 00:00:00

abstract：:Bloom's syndrome (BS) is an autosomal recessive disorder characterized by a strong cancer predisposition. The defining feature of BS is extreme genome instability. The gene mutated in Bloom's syndrome, BLM, encodes a DNA helicase (BLM) of the RecQ family. BLM plays a role in homologous recombination; however, its exac...

journal_title：Genes & development

authors： Bugreev DV,Yu X,Egelman EH,Mazin AV

更新日期：2007-12-01 00:00:00

abstract：:Endospores of Bacillus subtilis are encased in a two-layer protein shell known as the coat, which consists of a lammellar-like inner layer and an electron-dense outer layer. We report the cloning of the structural gene (designated cotE) for an alkali-soluble coat protein of 24 kD and show that the cotE gene product is...

journal_title：Genes & development

authors： Zheng LB,Donovan WP,Fitz-James PC,Losick R

更新日期：1988-08-01 00:00:00

abstract：:The empty spiracles (ems) homeo box gene is required for the development of the Drosophila larval filzkörper, which are structural specializations of the eighth abdominal segment. Filzkörper development is also dependent on the function of the homeotic selector gene Abdominal-B (Abd-B). Here, we show that ems is a dow...

journal_title：Genes & development

authors： Jones B,McGinnis W

更新日期：1993-02-01 00:00:00