Abstract:
:Metalloproteinase inhibitors, such as tissue inhibitors of metalloproteinases 1 and 2 (TIMP-1 and -2), play a key role in the regulation of metalloproteinases that modify extracellular matrix composition. Although expression of TIMP-1 within ovarian tissues has been well characterized, little information is available regarding expression of TIMP-2. The objective of the present studies was to characterize the ontogeny and localization of TIMP-2 messenger RNA (mRNA) within ovine preovulatory follicles and luteal tissue. Total cellular RNA was isolated from preovulatory follicles collected before (presurge; n = 3), or 12-14 h after (post surge; n = 4) an LHRH-induced gonadotropin surge, and from luteal tissue collected on days 3, 7, 10, 13, and 16 post estrus (n = 5, 5, 4, 5, and 5, respectively). TIMP-2 mRNA was expressed by both presurge and postsurge follicles, and expression did not increase after the gonadotropin surge (P = 0.44). In situ hybridization localized TIMP-2 mRNA primarily to the thecal layer of post-surge follicles (n = 3). TIMP-2 mRNA was also localized in a heterogeneous distribution within corpora lutea collected on days 3 and 10 post estrus (n = 3 each). Concentrations of TIMP-2 mRNA (picograms per microgram tissue DNA) were greater in corpora lutea collected during the early luteal phase (days 3 and 7) than the late luteal phase (day 16; P < 0.05). TIMP-2 mRNA was detected in purified populations of both small (n = 4) and large (n = 3) luteal cells, and mRNA concentrations (picograms per microgram DNA) were greater in the large luteal cells (P < or = 0.0002). In addition, immunoreactive TIMP-2 (approximately 21,000 M(r)) was detected by Western blot analysis of ovine luteal cell secreted proteins. We conclude that 1) TIMP-2 mRNA is expressed by the thecal layer of ovine preovulatory follicles and expression is not increased by the preovulatory gonadotropin surge; 2) expression of TIMP-2 mRNA is maximal during the early luteal phase; and 3) expression of TIMP-2 mRNA is greatest in large luteal cells.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Smith GW,McCrone S,Petersen SL,Smith MFdoi
10.1210/endo.136.2.7835290subject
Has Abstractpub_date
1995-02-01 00:00:00pages
570-6issue
2eissn
0013-7227issn
1945-7170journal_volume
136pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Epidermal growth factor (EGF) and TGFalpha are well known for their activities in the ovary. Both factors initiate signal transduction by binding to the common EGF receptor that has been demonstrated in the ovary across vertebrates from fish to humans. Using zebrafish as the model, we demonstrated in the present study...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.1.8579
更新日期:2002-01-01 00:00:00
abstract::Androgens are required for the development and function of the prostate. In a normal human prostate, androgens control the synthesis of proteins such as prostate-specific antigen and human glandular kallikrein. The prostate secretes these proteins as well as a number of other compounds to form the prostatic fluid. Usi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.4.5926
更新日期:1998-04-01 00:00:00
abstract::The secretion of LH, PRL, and cortisol was investigated in 4 sexually mature female rhesus macaques with cardiac catheters protected by tethers. Based on endocrine parameters, all 4 of the animals ovulated within 2 months from the time they were tethered, and regular menstrual cycles of 24-34 days were observed. The c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-1-261
更新日期:1984-07-01 00:00:00
abstract::Previous studies showed that noncalcemic 20(OH)D3, a product of CYP11A1 action on vitamin D3, has antifibrotic activity in human dermal fibroblasts and in a bleomycin mouse model of scleroderma. In this study, we tested the role of retinoic acid-related orphan receptor γ (RORγ), which is expressed in skin, in the acti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa198
更新日期:2021-01-01 00:00:00
abstract::The lipolysis, ketosis and hypertriglyceridemia of fasted rats in late pregnancy may be related to decreased insulin secretion geared to decreased glucose availability. In odd-carbon fatty acid enriched (OCE) rats, the odd-carbon fatty acids (OCFA) mobilized during fasting provide terminal three-carbon residues which ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-2-373
更新日期:1977-02-01 00:00:00
abstract::We have measured the concentrations of vasopressin in sequential samples of cerebrospinal fluid (CSF) obtained from fetal lambs between 108 and 130 days gestation. There was a clear and significant (F = 4.46; P less than 0.005) rhythm in vasopressin concentrations in the CSF of nine fetuses, characterized by a mean tr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-6-3095
更新日期:1989-06-01 00:00:00
abstract::Homologous anterior pituitaries grafted under the kidney capsule in hypophysectomized rats were studied 30 days after transplantation. Some cells maintained the ultrastructural features peculiar to the various cell types of normotopic glands, while the others were characterized by few, small, dense granules, spherical...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-1-226
更新日期:1979-01-01 00:00:00
abstract::Changes in serum and pituitary LH and FSH concentrations have been measured in the newborn male rat before, during, and up to 24 h after birth. A sudden and transient increase of serum and pituitary gonadotropins is observed at birth, which is followed by a rapid increase of absolute and relative testicular weights be...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-6-1985
更新日期:1978-12-01 00:00:00
abstract::The importance of locally produced insulin-like growth factor-I (IGF-I) in connective tissues has recently been recognized. It has been postulated that the action of anabolic hormones on bone may be mediated through local IGF-I release. However, whether IGF-I can also be modulated by other locally acting cytokines has...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-4-1635
更新日期:1990-10-01 00:00:00
abstract::Thyroid papillary carcinoma is the most common type of endocrine cancer. It is frequently associated with genetic alterations leading to activation of the MAPK signaling pathway. The two most frequently affected genes, BRAF and RET, are activated by either point mutation or as a result of chromosomal rearrangement. Th...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2006-0921
更新日期:2007-03-01 00:00:00
abstract::Somatostatin has been demonstrated to negatively regulate pancreatic growth in vivo. In this study we used the AR4-2J rat pancreatic acinar tumor cell line to investigate the effect of a stable somatostatin analog, SMS 201-995 (SMS) on cell proliferation. SMS induced an antiproliferative effect on both serum or epider...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-2-1017
更新日期:1989-02-01 00:00:00
abstract::Hashimoto thyroiditis can be partially reproduced in mice by immunization with thyroglobulin or, more recently, thyroperoxidase. This experimental autoimmune thyroiditis (EAT) model has been extensively characterized during early disease phases (up to d 35 after immunization). By extending the analysis of EAT to 100 d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0639
更新日期:2009-01-01 00:00:00
abstract::Neurotensin (NT) is a peptide expressed in the brain and in the gastrointestinal tract. Brain NT inhibits food intake, but the effects of peripheral NT are less investigated. In this study, peripheral NT decreased food intake in both mice and rats, which was abolished by a NT antagonist. Using c-Fos immunohistochemist...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1329
更新日期:2016-09-01 00:00:00
abstract::Estrogen is generally considered to maintain bone mass through suppression of bone resorption. We have previously demonstrated that administration of pharmacological doses of estrogen increases bone formation in rats. Because such high doses of estrogen might induce bone formation through some mechanism other than the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.5.1572310
更新日期:1992-05-01 00:00:00
abstract::PTH increases calcium reabsorption exclusively in the distal nephron. Two mechanisms of Ca++ transport through the basolateral membrane (BLM) have been described: the ATP-dependent and the sodium gradient-dependent transport. In the present study, we investigated the effect of PTH and (Bu)2cAMP on these two mechanisms...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-1-251
更新日期:1991-01-01 00:00:00
abstract::The master circadian clock located within the hypothalamic suprachiasmatic nucleus (SCN) is necessary for the circadian rhythm of glucocorticoid (GC) release. The pathways by which the SCN sustains rhythmic GC release remain unclear. We studied the circadian regulation of cortisol release in the behaviorally split gol...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1624
更新日期:2012-02-01 00:00:00
abstract::At menopause, the dramatic loss of ovarian estradiol (E2) necessitates the adaptation of estrogen-sensitive neurons in the hypothalamus to an estrogen-depleted environment. We developed a rat model to test the "critical window" hypothesis of the effects of timing and duration of E2 treatment after deprivation on the h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1032
更新日期:2015-08-01 00:00:00
abstract::PRL gene expression in the anterior pituitary has been the focus of intensive investigation for many years, but very little information is available on the actual dynamics by which this process occurs in individual mammotrope cells. Here, we used single cell bioluminescent imaging microscopy and a recently refined rep...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.3.8682
更新日期:2002-03-01 00:00:00
abstract::Recombinant interleukin-1 alpha and -beta (Il-1 alpha and -beta) have been evaluated for their abilities to stimulate ACTH and catecholamine secretion in the intact adult male rat. Additionally, the role of adrenergic-dependent pathways in mediating Il-1-induced ACTH release has been assessed. The iv or intracerebrove...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-6-3096
更新日期:1989-12-01 00:00:00
abstract::In Teleost fish, development, growth, and reproduction are influenced by the daily and seasonal variations of photoperiod and temperature. Early in vivo studies indicated the pineal gland mediates the effects of these external factors, most probably through the rhythmic production of melatonin. The present investigati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0707
更新日期:2003-10-01 00:00:00
abstract::The inhibitory effect of stress on reproductive function is potentially mediated by high concentrations of circulating glucocorticoids (GCs) acting via the GC receptor (GR). Gonadotropin-inhibitory hormone (GnIH) is a hypothalamic neuropeptide that inhibits gonadotropin secretion. GnIH may mediate stress-induced repro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-2076
更新日期:2014-05-01 00:00:00
abstract::In mammals, lactation is associated with a period of infertility characterized by the loss of pulsatile secretion of GnRH and cessation of ovulatory cycles. Despite the importance of lactational infertility in determining overall fecundity of a species, the mechanisms by which the suckling stimulus suppresses GnRH sec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1621
更新日期:2014-01-01 00:00:00
abstract::Oxytocin (OT) is released by the posterior pituitary during male orgasm and is supposed to participate in the ejaculatory process. We now report evidence demonstrating the presence of an OT receptor gene (real-time RT-PCR and Northern blot) and protein (immunohistochemistry, Western blot, and binding studies) expressi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0962
更新日期:2004-04-01 00:00:00
abstract::Hypothalamic proopiomelanocortin (POMC)-derived MSH peptides and the melanocortin receptor antagonist, agouti-related protein (AgRP), interact to regulate energy balance. Both POMC and AgRP neurons express estrogen receptors, but little is known about estrogen regulation of the melanocortin system in the primate. We h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0853
更新日期:2010-03-01 00:00:00
abstract::The possible role of atrial natriuretic factor (ANF) in the regulation of adrenal sensitivity to angiotensin II (AII) was investigated in vivo and in vitro by analyzing the characteristics of the inhibitory effect of ANF on aldosterone production stimulated by AII and other stimuli. In isolated adrenal glomerulosa cel...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-1-299
更新日期:1987-01-01 00:00:00
abstract::Cultured astroglial cells were found to contain a type II 5'-deiodinase (5'D) activity which was increased by 10(-3) M (Bu)2cAMP but not by 2 X 10(-3) M n-butyrate. 8-Bromo-cAMP (8-Br-cAMP) (10(-3) M) also increased this enzyme activity. Cycloheximide (2 micrograms/ml) inhibited the 8-Br-cAMP effect on 5'D activity. F...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-3-1577
更新日期:1988-09-01 00:00:00
abstract::The influence of periparturient secretion of PRL on cytological differentiation of mammary epithelial cells was studied in 17 multiparous, monotocous Holstein cows. Mammary tissue was obtained 10 days prepartum and 10 days postpartum from untreated cows and from cows treated with CB154 (2-Br-alpha-ergokryptin) or CB15...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-1-31
更新日期:1981-07-01 00:00:00
abstract::Stimulation of rat granulosa cell aromatase activity by FSH has recently been used as a sensitive biological end point to develop an in vitro FSH bioassay. The present report provides a detailed validation and application of this assay. In the presence of androstenedione and diethylstilbestrol, FSH stimulated estrogen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-4-1570
更新日期:1986-10-01 00:00:00
abstract::Immunohistochemical localization of two estrogen receptor (ER) subtypes, ER beta and ER alpha, was performed in neonatal, early postnatal, immature, and adult rats to determine whether ER alpha and ER beta are differentially expressed in the ovary. ER beta and ER alpha were visualized using a polyclonal anti-ER beta a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6533
更新日期:1999-02-01 00:00:00
abstract::We have analyzed the factors responsible for the circadian variation in rat hepatic mRNA-S14. Regulation of this sequence, which is found in lipogenic tissues and encodes a protein (S14) believed to be associated with fatty acid synthesis, is an excellent model of the interaction of thyroid hormone and dietary factors...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-4-1563
更新日期:1987-04-01 00:00:00