Abstract:
:At menopause, the dramatic loss of ovarian estradiol (E2) necessitates the adaptation of estrogen-sensitive neurons in the hypothalamus to an estrogen-depleted environment. We developed a rat model to test the "critical window" hypothesis of the effects of timing and duration of E2 treatment after deprivation on the hypothalamic neuronal gene network in the arcuate nucleus and the medial preoptic area. Rats at 2 ages (reproductively mature or aging) were ovariectomized and given E2 or vehicle replacement regimes of differing timing and duration. Using a 48-gene quantitative low-density PCR array and weighted gene coexpression network analysis, we identified gene modules differentially regulated by age, timing, and duration of E2 treatment. Of particular interest, E2 status differentially affected suites of genes in the hypothalamus involved in energy balance, circadian rhythms, and reproduction. In fact, E2 status was the dominant factor in determining gene modules and hormone levels; age, timing, and duration had more subtle effects. Our results highlight the plasticity of hypothalamic neuroendocrine systems during reproductive aging and its surprising ability to adapt to diverse E2 replacement regimes.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Yin W,Maguire SM,Pham B,Garcia AN,Dang NV,Liang J,Wolfe A,Hofmann HA,Gore ACdoi
10.1210/en.2015-1032subject
Has Abstractpub_date
2015-08-01 00:00:00pages
2918-33issue
8eissn
0013-7227issn
1945-7170pii
10.1210/en.2015-1032journal_volume
156pub_type
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