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Induction of type II 5'-deiodinase activity by cyclic adenosine 3', 5'-monophosphate in cultured rat astroglial cells.

Abstract：

:Cultured astroglial cells were found to contain a type II 5'-deiodinase (5'D) activity which was increased by 10(-3) M (Bu)2cAMP but not by 2 X 10(-3) M n-butyrate. 8-Bromo-cAMP (8-Br-cAMP) (10(-3) M) also increased this enzyme activity. Cycloheximide (2 micrograms/ml) inhibited the 8-Br-cAMP effect on 5'D activity. Forskolin (10(-5) M), cholera toxin (5 micrograms/ml), 10(-5) M isoproterenol, and 3 X 10(-6) M norephinephrine also increased the 5'D activity of astroglial cells. After a 4-h incubation these agents or cAMP analogs had maximal effect, and enzyme activities were 6- to 14-fold above control value. The stimulatory effects of isoproterenol and norepinephrine were almost completely reversed after 8 h incubation. The induction of 5'D activity by isoproterenol or norepinephrine was inhibited by the beta-adrenergic antagonist alprenolol (5 X 10(-6) M). The effect of norepinephrine was not significantly affected by the alpha 1-adrenergic antagonist, prazosin (10(-5) M). Thus, 5'D activity is controlled by agents increasing cAMP in astroglial cells, and in particular by the neurotransmitter, norephinephrine, via a beta-adrenergic mechanism.

journal_name

Endocrinology

journal_title

Endocrinology

authors

Courtin F,Chantoux F,Pierre M,Francon J

doi

10.1210/endo-123-3-1577

subject

Has Abstract

pub_date

1988-09-01 00:00:00

pages

1577-81

issue

3

eissn

0013-7227

issn

1945-7170

journal_volume

123

pub_type

杂志文章

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