Abstract:
:Estrogens play a central role in regulating female reproduction throughout the reproductive axis, and the pituitary is one of the major targets of estrogen action. We hypothesized that estrogen receptor alpha (ERalpha) mediates estrogen action in the pituitary gonadotroph. To test this hypothesis, we generated a mouse line with a selective ERalpha deletion in the gonadotropin alpha-subunit (alphaGSU)-expressing pituitary cells (pituitary-specific ERalpha knockout; ERalpha(flox/flox) alphaGSU(cre)). Although the ERalpha(flox/flox) alphaGSU(cre) female mice maintain a basal level of serum LH and FSH and their ovulatory capacity is comparable to that in controls, they do not display regular estrous cycles and are infertile, indicating a potential disorder in regulating LH and/or FSH secretion. The ERalpha(flox/flox) alphaGSU(cre) female mice express equivalent levels of LHbeta and alphaGSU mRNA compared with wild-type mice as determined by microarray analysis. Taken together, these findings indicate that pituitary gonadotroph ERalpha carries out the effects of estrogens with regard to estrous cyclicity and ultimately fertility.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Gieske MC,Kim HJ,Legan SJ,Koo Y,Krust A,Chambon P,Ko Cdoi
10.1210/en.2007-1084subject
Has Abstractpub_date
2008-01-01 00:00:00pages
20-7issue
1eissn
0013-7227issn
1945-7170pii
en.2007-1084journal_volume
149pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Continuous labeling of primary cultures of human syncytiotrophoblasts with [125I]iodoepidermal growth factor (iodo-EGF) at 37 C revealed a progressive increase in intracellular EGF with 5% of total label observed in intracellular organelles at 2 min and 88% by 60 min. Detailed analysis by electron microscope radioauto...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-413
更新日期:1986-01-01 00:00:00
abstract::Interleukin-6 (IL-6) stimulates the release of hCG from syncytiotrophoblast cells, but the effects of IL-6 and other cytokines on the release of placental lactogen (hPL) are unknown. To determine the effect of IL-6 on hPL release, we exposed an enriched fraction of trophoblast cells (prepared by the isopycnic centrifu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.2.8033820
更新日期:1994-08-01 00:00:00
abstract::Synthesis of GH, PRL, and proopiomelanocortin (POMC) by the adult ovine pituitary was examined by culturing intact tissue explants in vitro and analyzing newly synthesized proteins by two-dimensional gel electrophoresis. Spots on autoradiographs of two-dimensional gels were identified by comigration with known standar...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-4-1358
更新日期:1982-10-01 00:00:00
abstract::Activin, a member of the transforming growth factor-beta superfamily, binds to two classes of cell surface receptors. These receptors, designated type I and type II, are structurally related members of transmembrane serine kinase superfamily. Antibodies specific for either type I or type II activin receptor can coprec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.12.7588300
更新日期:1995-12-01 00:00:00
abstract::We previously reported that TNF-alpha-induced apoptosis of lactotropes is estrogen dependent and predominant at proestrus. Here we observed that TNF-alpha (50 ng/ml) failed to induce apoptosis of anterior pituitary cells from ovariectomized rats cultured in the presence of progesterone (10(-6) m). However, progesteron...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1276
更新日期:2005-02-01 00:00:00
abstract::To study the transcriptional regulation of the vasopressin gene in vitro, 3 kb of the 5' regulatory region of the rat vasopressin gene was isolated and subcloned, along with a series of various deletion mutants, into vectors containing the luciferase reporter gene. After transfecting these genes transiently into the h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.12.5639
更新日期:1997-12-01 00:00:00
abstract::Medullary thyroid cancer (MTC) is a C cell neoplasm-secreting calcitonin. Surgery remains the only treatment as the primary tumor and metastases resist radio- and chemotherapies. MTC produces high amounts of prostaglandins (PGs). Nonsteroidal antiinflammatory drugs have an antitumoral effect, generally related to the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0915
更新日期:2004-05-01 00:00:00
abstract::Nutritional programming, taking place in utero or early after birth, is closely linked with metabolic and appetite disorders in adulthood. Following the hypothesis that nutritional programming impacts hypothalamic neuronal organization, we report on discrepancies of multiple molecular and cellular early events that ta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0893
更新日期:2010-02-01 00:00:00
abstract::Cholecystokinin (CCK)/gastrin receptors were characterized in calf pancreatic plasma membranes from newborns, 28- and 119-day-old milk-fed preruminants, and 119-day-old weaned ruminants. Scatchard analysis of [125I]Bolton-Hunter reagent-[Thr28,Nle31]CCK-(25-33) binding indicated two classes of binding sites: high affi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.3.8365360
更新日期:1993-09-01 00:00:00
abstract::LH receptor (LHR) expression in the ovary is regulated by the RNA binding protein, (LHR mRNA binding protein [LRBP]), which has been identified as being mevalonate kinase. This study examined the role of microRNA miR-122 in LRBP-mediated LHR mRNA expression. Real-time PCR analysis of ovaries from pregnant mare serum g...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1619
更新日期:2013-12-01 00:00:00
abstract::The PTH and PTH-related protein (PTHrP) system consists of two hormones, at least two G protein-coupled seven-transmembrane domain receptors, and at least two intracellular signal transduction pathways for each receptor. The PTH/PTHrP receptor is present in the conventional target tissues of PTH action, namely kidney ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.10.8828480
更新日期:1996-10-01 00:00:00
abstract::Although the interactions between sex steroids and GnRH have been extensively studied, little is known about the mechanism of estradiol (E2) effects on GnRH secretion. In the present study, we used retrochiasmatic hypothalamic explants of 50-d-old male rats, and we observed that E2 significantly increased the glutamat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1265
更新日期:2005-05-01 00:00:00
abstract::A central feature of glucocorticoid (GC)-induced osteoporosis is decreased bone formation, secondary to decreased numbers of functional osteoblasts. We find that ERK activity is essential for serum-induced osteoblast proliferation in vitro because inhibition of MAPK/ERK kinase activity by U0126 completely abolished bo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220769
更新日期:2003-02-01 00:00:00
abstract::Mammary glands from second generation vitamin D-deficient mice and rats were examined for their ability to make the major milk proteins, casein and alpha-lactalbumin, both in vivo and in vitro. The glands from the rachitic animals were morphologically indistinguishable from those of age-matched controls. When placed i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-121-3-865
更新日期:1987-09-01 00:00:00
abstract::Thyroid toxicity of iodide excess has been demonstrated in animals fed with an iodide-rich diet; in vitro iodide is cytotoxic, inhibits cell growth, and induces morphological changes in thyroid cells of some species. In this study, we investigated the effect of iodide excess in an immortalized thyroid cell line (TAD-2...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.2.7291
更新日期:2000-02-01 00:00:00
abstract::The median eminence (ME), which is the common termination field for adenohypophysiotropic systems, has been shown to produce nitric oxide (NO), a signaling molecule involved in neuroendocrine secretion. Using an ex vivo technique, 17beta-estradiol exposure to ME fragments, including vascular tissues, stimulated NO rel...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6484
更新日期:1999-02-01 00:00:00
abstract::The distribution of peptide YY (PYY)-like immunoreactivity (IR) in rat tissues was determined by specific RIA after extraction with boiled 1 N acetic acid. The high concentration of PYY-IR was observed in the gastrointestinal tract, with concentrations gradually increasing from the duodenum to the end of colon. The co...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-6-2163
更新日期:1986-06-01 00:00:00
abstract::Removal of the 18-hydroxy group of the hemiacetal form of aldosterone transforms its activity from that of pure agonist to predominant antagonist. The 18-deoxy derivative possesses one third of the binding affinity of aldosterone for the cytoplasmic mineralocorticoid receptor of rat kidney and exhibits an approximate ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-104-5-1352
更新日期:1979-05-01 00:00:00
abstract::We have reported that calcitonin (CT) treatment induced downregulation of the CT receptor (CTR) in mouse osteoclast-like cells (OCLs). Here, we studied the features of homologous down-regulation of the CTR in mature mouse OCLs. Treatment with salmon CT (sCT) and human CT (hCT) reduced [125I]sCT specific binding. The d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.1.8536630
更新日期:1996-01-01 00:00:00
abstract::The brain has traditionally been considered to be a target site of peripheral steroid hormones. By contrast, new findings over the past decade have shown that the brain itself also has the capability of forming steroids de novo, the so-called neurosteroids. De novo neurosteroidogenesis in the brain from cholesterol is...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2007-1592
更新日期:2008-06-01 00:00:00
abstract::A1/A2 noradrenergic neurons in the medulla oblongata are well known to mediate stress signals in the central nervous system. Stress activates A1/A2 noradrenergic neurons, and then noradrenaline (NA) stimulates ACTH secretion through hypothalamic CRH. On the other hand, PRL-releasing peptide (PrRP) was recently isolate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.5.8118
更新日期:2001-05-01 00:00:00
abstract::The effects of endothelin-1 (ET) on several tissues are mediated by prostaglandins. In this study, we investigated the actions of ET on bone and determined whether they are mediated through prostaglandin-dependent pathways. Bone resorption, collagen, and non-collagen protein synthesis and inositol phosphate (IP) produ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.2.1639010
更新日期:1992-08-01 00:00:00
abstract::Estrogens have pronounced effects on thermoregulation, as illustrated by the occurrence of hot flushes secondary to estrogen withdrawal in menopausal women. Because neurokinin B (NKB) gene expression is markedly increased in the infundibular (arcuate) nucleus of postmenopausal women, and is modulated by estrogen withd...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1492
更新日期:2011-12-01 00:00:00
abstract::The factors controlling the synthesis and degradation of the insulin-like growth factor-binding proteins (IGFBPs) during pregnancy are poorly understood. To clarify the roles of nutritional factors in the regulation of fetal and maternal IGFBP production, we examined the effects of fasting, refeeding, and glucose admi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.4.1382960
更新日期:1992-10-01 00:00:00
abstract::To determine if oxytocin (OT) is present in cynomolgus monkey corpus luteum, OT was measured by a specific and sensitive RIA in 13 corpora lutea, ovarian venous plasma on the ipsilateral side and peripheral venous plasma at different stages of the luteal phase. Serial dilution of acetic acid extract of the corpus lute...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-2-570
更新日期:1984-08-01 00:00:00
abstract::The metabolism of cholesterol sulfate (5-cholesten-3 beta-ol sodium sulfate) and cholesterol to pregnenolone sulfate and pregnenolone, respectively, in human fetal adrenal mitochondria has been investigated. Cholesterol sulfate (50 microM) was converted efficiently to pregnenolone sulfate (5-pregnen-3 beta-ol-20-one s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-1-208
更新日期:1982-07-01 00:00:00
abstract::LA-PTH is a long-acting parathyroid hormone (PTH) peptide analogue in preclinical development for hypoparathyroidism (HP). Like native PTH, LA-PTH contains a methionine at position 8 (Met8) that is predicted to be critical for function. We assessed the impact of Met oxidation on the functional properties of LA-PTH and...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa216
更新日期:2021-02-01 00:00:00
abstract::Mineralocorticoid and glucocorticoid hormones are metabolized as glucuronide conjugates. Using labeled [(14)C]uridine diphosphate glucuronic acid and microsomal preparations from human embryonic kidney 293 cells stably expressing the different human and monkey uridine diphosphate glucuronosyltransferase (UGT)2B enzyme...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-0052
更新日期:2003-06-01 00:00:00
abstract::The endoplasmic reticulum (ER) mediates the first steps of protein assembly within the secretory pathway and is the site where protein folding and quality control are initiated. The storage and release of Ca2+ are critical physiological functions of the ER. Disrupted ER homeostasis activates the unfolded protein respo...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/endocr/bqz028
更新日期:2020-02-01 00:00:00
abstract::In seasonally breeding species, an animal's photoperiodic history (the daylength or photoperiod previously experienced) influences the reproductive response to new photoperiods. However, this has only been examined over a relatively narrow range of photoperiods. We assessed whether Djungarian hamsters (Phodopus sungor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-6-2634
更新日期:1988-06-01 00:00:00