Abstract:
:There is considerable interest in immunohistochemical markers of proliferation which are suitable for use on routinely fixed clinical material. The novel proliferation-associated antibody Ki-S1 shows promise in this respect. In this study we have: (i) defined the pattern of Ki-S1 labelling relative to the cell cycle phase; (ii) investigated the labelling pattern with Ki-S1 on a human breast cell line (ZR75) under varying proliferative conditions induced by serum deprivation and refeeding; (iii) examined in a flow cytometric study Ki-S1 staining in archival, clinical breast carcinoma samples. In exponentially growing cells Ki-S1 showed a marked cell cycle phase-specific variation in staining intensity which increased linearly through the S-phase, was high in G2 and reached its peak in mitosis. Ki-S1 staining intensity mirrored the changes in proliferative activity of ZR75 cells during serum deprivation and refeeding. In a small series of human breast carcinoma, Ki-S1 staining intensity correlated with S-phase fraction (SPF) derived from DNA profiles. The antigen labelled by Ki-S1 is extremely robust, resisting degradation by fixation and by an aggressive enzymic tissue disaggregation method. Ki-S1 warrants further investigation as a proliferation-related marker, particularly for routine clinical application.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Camplejohn RS,Brock A,Barnes DM,Gillett C,Raikundalia B,Kreipe H,Parwaresch MRdoi
10.1038/bjc.1993.122subject
Has Abstractpub_date
1993-04-01 00:00:00pages
657-62issue
4eissn
0007-0920issn
1532-1827journal_volume
67pub_type
杂志文章abstract::Novel synthetic antagonists of retinoic acid receptors (RARs) have been developed. To avoid interference by serum retinoids when testing these compounds, we established serum-free grown sub-lines (>3 years) of the prostate carcinoma lines LNCaP, PC3 and DU145. A high affinity pan-RAR antagonist (AGN194310, K(d) for bi...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.2001.1939
更新日期:2001-08-03 00:00:00
abstract::The micronucleus (MN) test has been carefully characterized in four human tumour cell lines of widely differing radiosensitivity. Two radioresistant bladder carcinoma cell lines (MGH-U1 and RT112), one sensitive medulloblastoma cell line (D283MED) and a sensitive neuroblastoma cell line (HX142) were used. The number o...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1993.17
更新日期:1993-01-01 00:00:00
abstract::The murine dose of i.v. C. parvum (466 microgram) was compared with a single, low, human-equivalent dose of 70 microgram and with repeated weekly low doses. All treatments increased the antibody titre against C. parvum (CP). However, repeated doses stimulated a much higher titre than single doses. In all treated anima...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1980.64
更新日期:1980-03-01 00:00:00
abstract::The matrix metalloprotease MMP-9 localizes to tumour-associated macrophages in human ovarian cancer but little is known of its regulation. Co-culture of human ovarian cancer cells (PEO-1) and a monocytic cell line (THP-1) led to production of 92-kDa proMMP-9. PEO-1-conditioned medium (CM) also stimulated THP-1 cells o...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.568
更新日期:1998-09-01 00:00:00
abstract::Identification of a biomarker of prognosis and response to therapy that can be assessed preoperatively would significantly improve overall outcomes for patients with pancreatic cancer. In this study, patients whose tumours exhibited high LMO4 expression had a significant survival advantage following operative resectio...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604177
更新日期:2008-02-12 00:00:00
abstract::To invade and metastasize, carcinomas must penetrate or lose their epithelial basement membrane (EBM), and then penetrate basement membranes (BMs) surrounding blood vessels, lymphatics, nerves and muscle cells. Knowledge of the composition of different BMs is necessary, so that appropriate antibodies and DNA probes ar...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.37
更新日期:1997-01-01 00:00:00
abstract::The aim of this work was to test in vitro and in vivo the efficacy of the derivatives of 5-aminolevulinic acid (ALA): hexyl-ALA (He-ALA), undecanoyl-ALA and R,S-2-(hydroximethyl)tetrahydropyranyl-ALA (THP-ALA) as pro-photosensitising agents. The compounds were assayed in a cell line derived from a murine mammary tumou...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601722
更新日期:2004-04-19 00:00:00
abstract:BACKGROUND:Cancer prognostic biomarkers have shown disappointing clinical applicability. The objective of this study was to classify and estimate how study results are overinterpreted and misreported in prognostic factor studies in oncology. METHODS:This systematic review focused on 17 oncology journals with an impact...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-018-0305-5
更新日期:2018-11-01 00:00:00
abstract::Increasingly, reverse transcriptase polymerase chain reaction (RT-PCR) is used to detect clinically significant tumour cells in blood or bone marrow. This may result in a redefinition of disease-free and clinical relapse. However, its clinical utility may be limited by lack of automation or reproducibility. Recent stu...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600014
更新日期:2002-01-07 00:00:00
abstract::Diagnosis of hereditary non-polyposis colorectal cancer (HNPCC) is currently based on phenotypical analysis of an expanded pedigree. Diagnostic guidelines ('Amsterdam criteria') proposed by the International Collaborative Group on HNPCC are often too stringent for use with small families. There is also the possibility...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1995.508
更新日期:1995-11-01 00:00:00
abstract:BACKGROUND:Activins and inhibins belong to the TGFβ-superfamily, which controls cell proliferation and differentiation in many organs. Activin A, the dimer of inhibin βA subunit, acts strongly anti-proliferative in hepatocytes. Little is known on the other activin/inhibin subunits in human liver and hepatocellular carc...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2011.53
更新日期:2011-04-12 00:00:00
abstract::To estimate the prevalence of TP53 mutations in familial breast cancer, constant denaturant gel electrophoresis (CDGE) was used to screen exons 5-8 of the TP53 gene for germline mutations. Genomic DNA from 128 breast cancer patients belonging to 109 families with familial cancer were screened. No germline mutations we...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.205
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Mechanisms that increase resistance to apoptosis help promote cellular transformation. Cancer cells have deregulated apoptotic pathways, where increased expression and stability of anti-apoptotic proteins Mcl-1 and Bcl-2 increases resistance to apoptosis. Pathways that increase the stability of proteins in c...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2011.242
更新日期:2011-07-26 00:00:00
abstract::Metabolic reprogramming towards aerobic glycolysis is a common feature of transformed cells and can be driven by a network of transcription factors. It is well established that c-Myc and hypoxia-inducible factor-1α (HIF-1α) contribute to metabolic reprogramming by driving the expression of glycolytic target genes. Mor...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/bjc.2015.360
更新日期:2015-12-01 00:00:00
abstract::Increased expression of tumour-associated trypsin inhibitor (TATI) in tumour tissue and/or serum has been associated with poor survival in various cancer forms. Moreover, a proinvasive function of TATI has been shown in colon cancer cell lines. In this study, we have examined the prognostic significance of tumour-spec...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605047
更新日期:2009-05-19 00:00:00
abstract:BACKGROUND:Alternative treatments are needed for elderly patients with acute myeloid leukaemia, as the disease prognosis is poor and the current treatment is unsuitable for many patients. METHODS:In this study, we investigated whether combining the nucleoside analogue sapacitabine with histone deacetylase (HDAC) inhib...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605922
更新日期:2010-10-26 00:00:00
abstract:BACKGROUND:Tumour heterogeneity is documented for many characters, including the production of growth factors, one of the hallmarks of cancer. What maintains heterogeneity remains an open question that has implications for diagnosis and treatment, as drugs that target growth factors are susceptible to the evolution of ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.336
更新日期:2013-08-20 00:00:00
abstract::The aim of this study was to examine the effects of oral and transdermal oestrogen replacement therapy on the risk of colorectal cancer. Data from a nested case-control study, designed to investigate the effect of hormone replacement therapy (HRT) on colorectal cancer were analysed. New cases of colorectal cancer, dia...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601438
更新日期:2004-01-12 00:00:00
abstract::To investigate the association between medullary carcinoma of the thyroid (MCT) and carcinoembryonic antigen (CEA), we assayed 78 sera from patients with thyroid diseases for CEA, employing the radioimmunoassay of double antibody technique. All 13 sera from patients with MCT had high levels of CEA, ranging from 14 to ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1976.133
更新日期:1976-08-01 00:00:00
abstract::The effects of suramin, an inhibitor of growth factor mitogenic activity, were evaluated on basic fibroblast growth factor (bFGF)-induced proliferation of bovine aortic endothelial cells and on angiogenesis in the chorioallantoic membrane (CAM) of chick embryos. The role of bFGF gene expression in endothelial cell gro...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1993.457
更新日期:1993-11-01 00:00:00
abstract:BACKGROUND:Lymphovascular invasion (LVI) is associated with the development of metastasis in invasive breast cancer (BC). However, the complex molecular mechanisms of LVI, which overlap with other oncogenic pathways, remain unclear. This study, using available large transcriptomic datasets, aims to identify genes assoc...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-019-0486-6
更新日期:2019-06-01 00:00:00
abstract::The prognostic value of several clinical and histopathological characteristics has been evaluated in patients with Dukes' B and C carcinoma of the rectum and the rectosigmoid. Data on 260 Dukes' B and 208 Dukes' C tumours entered into a prospective, randomized clinical trial of post-operative radiotherapy (50 Gy given...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1988.192
更新日期:1988-08-01 00:00:00
abstract:BACKGROUND:It has been hypothesised that intrauterine exposures are important for subsequent prostate cancer risk. Prior epidemiological studies have used birthweight as a proxy of cumulative intrauterine exposures to test this hypothesis, but results have been inconsistent partly because of limited statistical power. ...
journal_title:British journal of cancer
pub_type: 杂志文章,meta分析,评审
doi:10.1038/bjc.2016.38
更新日期:2016-03-29 00:00:00
abstract:BACKGROUND:Based on the microRNA (miRNA) signature of bladder cancer (BC) by deep sequencing, we recently found that several double-stranded mature miRNAs derived from the same pre-miRNAs were sufficiently expressed and acted as tumour suppressors by regulating common target genes in BC. Our deep-sequencing signature o...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2017.43
更新日期:2017-04-11 00:00:00
abstract::We have studied whether N-methylformamide can affect the expression pattern of adhesion molecules and the attachment behaviour of M14 human melanoma cells. The role of N-methylformamide on experimental and spontaneous pulmonary metastases from M14 cells in nude mice was also investigated. We demonstrate that N-methylf...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.35
更新日期:1998-01-01 00:00:00
abstract::We have studied the ability of cyclosporin A (CsA) and a non-immunosuppressive analogue, O-acetyl cyclosporin A (OACsA, B3-243) to inhibit the growth of human lung cancer cells in vitro. Using continuous drug exposure and the MTT colorimetric assay to determine cell growth we found that CsA produced partial growth inh...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1992.68
更新日期:1992-03-01 00:00:00
abstract:BACKGROUND:Enzastaurin, an oral serine-threonine kinase inhibitor, was initially developed as an ATP-competitive selective inhibitor against protein kinase Cβ. However, the mechanism by which enzastaurin contributes to tumourigenesis remains unclear. METHODS:We analysed the anti-tumour effects of enzastaurin in 22 lun...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.7
更新日期:2012-02-28 00:00:00
abstract::To understand genetic events which play a role in the development and/or progression of human endometrial cancer, we studied allelotypes on all autosomal chromosomes, as well as chromosome X, with 42 microsatellite markers and 56 endometrial cancers. The most frequent loss of heterozygosity (LOH) was observed on the l...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.663
更新日期:1996-12-01 00:00:00
abstract::MicroRNAs (miRNAs) are small non-protein-coding RNAs that function as endogenous negative gene regulators. Dysfunctions of miRNAs are frequently found in malignancies, including lung cancer. In this review, we summarise the current understanding of miRNAs in lung cancer tumourigenesis, and highlight their potential in...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/sj.bjc.6605901
更新日期:2010-10-12 00:00:00
abstract:BACKGROUND:To compare the imaging and clinical features of temporal lobe necrosis (TLN) in nasopharyngeal carcinoma (NPC) patients treated with two-dimensional radiotherapy (2D-RT) or those with intensity-modulated radiotherapy (IMRT). METHODS:We retrospectively analysed NPC patients who underwent 2D-RT (72 patients, ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2014.243
更新日期:2014-05-27 00:00:00